MD14G1134500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005250
Physical Location & Seq
Forward (+)
21292546 .. 21292785
240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1134500.v1.1.491

Sequence Viewer

Length: 240 bp
ATGGAAAACGAGAGCAGTATCATATCCCGGGAGAAGCTTGACCAGGTGGCGAGCTGGCTCAGCGCCACCGTGTGCACCGCCTTCTTCTCCTCCCTCGAGCGCTTCTCTTGCGTCAACCTCTCGACTACCGACCTCGATGACGAGGACGAGGACGAGGACGAGGCCAAGGACCGCCCTCTCACCCTCACCAATTACAACAACAACGAACCTTCAAACGACGTCGCTAATCTCCCCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

80

Amino Acids

8.86

Weight (kDa)

4.05

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 222
AciI CCGC 2 cut(s) 78, 172
AcyI GRCGYC 1 cut(s) 219
AdeI CACNNNGTG 1 cut(s) 72
AfeI AGCGCT 1 cut(s) 101
AgsI TTSAA 1 cut(s) 213
AjnI CCWGG 1 cut(s) 42
AluBI AGCT 2 cut(s) 37, 54
AluI AGCT 2 cut(s) 37, 54
Alw21I GWGCWC 1 cut(s) 77
Alw44I GTGCAC 1 cut(s) 73
Ama87I CYCGRG 2 cut(s) 27, 95
Aor51HI AGCGCT 1 cut(s) 101
AoxI GGCC 1 cut(s) 162
ApaLI GTGCAC 1 cut(s) 73
AspLEI GCGC 2 cut(s) 65, 102
AspS9I GGNCC 1 cut(s) 169
AsuC2I CCSGG 2 cut(s) 28, 29
AsuHPI GGTGA 2 cut(s) 172, 178
AvaI CYCGRG 2 cut(s) 27, 95
AvaII GGWCC 1 cut(s) 169
BaeGI GKGCMC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 44
BcnI CCSGG 2 cut(s) 28, 29
BfoI RGCGCY 2 cut(s) 66, 103
BlpI GCTNAGC 1 cut(s) 59
Bme1390I CCNGG 3 cut(s) 28, 29, 44
Bme18I GGWCC 1 cut(s) 169
BmeT110I CYCGRG 2 cut(s) 27, 95
BmgT120I GGNCC 1 cut(s) 169
BmrFI CCNGG 3 cut(s) 28, 29, 44
BplI GAGNNNNNCTC 2 cut(s) 89, 121
Bpu1102I GCTNAGC 1 cut(s) 59
BpuMI CCSGG 2 cut(s) 28, 29
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 2 cut(s) 27, 165
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 2 cut(s) 27, 165
BseMII CTCAG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 79
BseSI GKGCMC 1 cut(s) 77
BshFI GGCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 77
BsiHKCI CYCGRG 2 cut(s) 27, 95
BsiSI CCGG 1 cut(s) 28
BsnI GGCC 1 cut(s) 164
BsoBI CYCGRG 2 cut(s) 27, 95
Bsp1286I GDGCHC 1 cut(s) 77
Bsp1720I GCTNAGC 1 cut(s) 59
BspACI CCGC 2 cut(s) 78, 172
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 1 cut(s) 72
BssECI CCNNGG 2 cut(s) 27, 165
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 1 cut(s) 70
BstACI GRCGYC 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 52, 56
BstDEI CTNAG 1 cut(s) 59
BstH2I RGCGCY 2 cut(s) 66, 103
BstHHI GCGC 2 cut(s) 65, 102
BstMWI GCNNNNNNNGC 2 cut(s) 60, 108
BstNI CCWGG 1 cut(s) 44
BstSCI CCNGG 3 cut(s) 26, 27, 42
BstSLI GKGCMC 1 cut(s) 77
BsuRI GGCC 1 cut(s) 164
Cac8I GCNNGC 2 cut(s) 52, 56
CfoI GCGC 2 cut(s) 65, 102
Cfr13I GGNCC 1 cut(s) 169
Cfr9I CCCGGG 1 cut(s) 27
CseI GACGC 1 cut(s) 100
CsiI ACCWGGT 1 cut(s) 42
CviJI RGCY 4 cut(s) 37, 54, 58, 164
CviKI_1 RGCY 4 cut(s) 37, 54, 58, 164
DdeI CTNAG 1 cut(s) 59
DraIII CACNNNGTG 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 165
Eco47I GGWCC 1 cut(s) 169
Eco47III AGCGCT 1 cut(s) 101
Eco88I CYCGRG 2 cut(s) 27, 95
EcoRII CCWGG 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
FaiI YATR 1 cut(s) 23
GlaI GCGC 2 cut(s) 64, 101
HaeII RGCGCY 2 cut(s) 66, 103
HaeIII GGCC 1 cut(s) 164
HapII CCGG 1 cut(s) 28
HgaI GACGC 1 cut(s) 100
HhaI GCGC 2 cut(s) 65, 102
Hin1I GRCGYC 1 cut(s) 219
Hin6I GCGC 2 cut(s) 63, 100
HinP1I GCGC 2 cut(s) 63, 100
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 35
HpaII CCGG 1 cut(s) 28
HphI GGTGA 2 cut(s) 172, 178
Hpy166II GTNNAC 2 cut(s) 75, 115
Hpy188I TCNGA 1 cut(s) 239
Hpy188III TCNNGA 1 cut(s) 121
Hpy8I GTNNAC 2 cut(s) 75, 115
Hpy99I CGWCG 2 cut(s) 221, 224
HpyAV CCTTC 2 cut(s) 91, 219
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 108
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 219
HspAI GCGC 2 cut(s) 63, 100
LpnPI CCDG 4 cut(s) 29, 40, 41, 56
MabI ACCWGGT 1 cut(s) 42
MaeII ACGT 1 cut(s) 219
MboII GAAGA 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 1 cut(s) 190
MspI CCGG 1 cut(s) 28
MspR9I CCNGG 3 cut(s) 28, 29, 44
MvaI CCWGG 1 cut(s) 44
MwoI GCNNNNNNNGC 2 cut(s) 60, 108
NciI CCSGG 2 cut(s) 28, 29
PaeR7I CTCGAG 1 cut(s) 95
Psp6I CCWGG 1 cut(s) 42
PspGI CCWGG 1 cut(s) 42
PspPI GGNCC 1 cut(s) 169
PspXI VCTCGAGB 1 cut(s) 95
Sau96I GGNCC 1 cut(s) 169
ScrFI CCNGG 3 cut(s) 28, 29, 44
SduI GDGCHC 1 cut(s) 77
SetI ASST 7 cut(s) 39, 48, 56, 120, 135, 211, 222
SexAI ACCWGGT 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 95
SinI GGWCC 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 95
SmaI CCCGGG 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 1 cut(s) 190
SsiI CCGC 2 cut(s) 78, 172
StyD4I CCNGG 3 cut(s) 26, 27, 42
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 1 cut(s) 222
TaqI TCGA 3 cut(s) 96, 122, 135
TasI AATT 1 cut(s) 190
TspMI CCCGGG 1 cut(s) 27
VneI GTGCAC 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 169
XhoI CTCGAG 1 cut(s) 95
XmaI CCCGGG 1 cut(s) 27
ZraI GACGTC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.