Rw7G006740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005250
Physical Location & Seq
Forward (+)
5756181 .. 5756800
620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G006740.1

Sequence Viewer

Length: 264 bp
ATGGAAGACGGCAACCCCAACCCCAATAACGCCATAATCTCCCGGGAGACGATTGACTCGGTCGCCGGCTGGGTCAGCGCCACCGTCTCCTCCGCCTTCTTCTCCTCCCTCGAGCGCTTCTCTTGCGTCAACGTCGCCACCACGGACGCCGACGAGGACGACGATGACGACGAGGCCGCCCACCGCCCTCTCGCTCTCACCGATCTCACCCACCCCGATCAACAGCACCACCACGCAAACGACGTCGCTCAGCTCCCCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

87

Amino Acids

9.41

Weight (kDa)

4.13

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 246
AciI CCGC 3 cut(s) 93, 177, 184
AcyI GRCGYC 2 cut(s) 147, 243
AfeI AGCGCT 1 cut(s) 116
AluBI AGCT 1 cut(s) 253
AluI AGCT 1 cut(s) 253
Alw26I GTCTC 2 cut(s) 41, 91
Ama87I CYCGRG 2 cut(s) 42, 110
Aor51HI AGCGCT 1 cut(s) 116
AoxI GGCC 1 cut(s) 174
AspLEI GCGC 2 cut(s) 80, 117
AsuC2I CCSGG 2 cut(s) 43, 44
AsuHPI GGTGA 2 cut(s) 190, 199
AvaI CYCGRG 2 cut(s) 42, 110
BbsI GAAGAC 1 cut(s) 12
BceAI ACGGC 1 cut(s) 25
BcnI CCSGG 2 cut(s) 43, 44
BcoDI GTCTC 2 cut(s) 41, 91
BfoI RGCGCY 2 cut(s) 81, 118
BisI GCNGC 1 cut(s) 177
BlpI GCTNAGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 2 cut(s) 43, 44
BmeT110I CYCGRG 2 cut(s) 42, 110
BmrFI CCNGG 2 cut(s) 43, 44
BpiI GAAGAC 1 cut(s) 12
BplI GAGNNNNNCTC 2 cut(s) 104, 136
Bpu1102I GCTNAGC 1 cut(s) 249
BpuMI CCSGG 2 cut(s) 43, 44
BsaHI GRCGYC 2 cut(s) 147, 243
BsaJI CCNNGG 2 cut(s) 42, 141
Bse118I RCCGGY 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 42, 141
BseMII CTCAG 1 cut(s) 263
BseRI GAGGAG 2 cut(s) 79, 94
BseYI CCCAGC 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 63
BsiHKCI CYCGRG 2 cut(s) 42, 110
BsiSI CCGG 2 cut(s) 43, 66
BsmAI GTCTC 2 cut(s) 41, 91
BsmBI CGTCTC 2 cut(s) 41, 91
BsnI GGCC 1 cut(s) 176
BsoBI CYCGRG 2 cut(s) 42, 110
Bsp143I GATC 2 cut(s) 202, 217
Bsp1720I GCTNAGC 1 cut(s) 249
BspACI CCGC 3 cut(s) 93, 177, 184
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 42, 141
BssMI GATC 2 cut(s) 202, 217
BssNI GRCGYC 2 cut(s) 147, 243
Bst4CI ACNGT 1 cut(s) 85
BstACI GRCGYC 2 cut(s) 147, 243
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 1 cut(s) 249
BstDSI CCRYGG 1 cut(s) 141
BstH2I RGCGCY 2 cut(s) 81, 118
BstHHI GCGC 2 cut(s) 80, 117
BstKTI GATC 2 cut(s) 205, 220
BstMAI GTCTC 2 cut(s) 41, 91
BstMBI GATC 2 cut(s) 202, 217
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 2 cut(s) 75, 123
BstSCI CCNGG 2 cut(s) 41, 42
BstV2I GAAGAC 1 cut(s) 12
BsuRI GGCC 1 cut(s) 176
BtgI CCRYGG 1 cut(s) 141
Cac8I GCNNGC 1 cut(s) 67
CfoI GCGC 2 cut(s) 80, 117
Cfr10I RCCGGY 1 cut(s) 65
Cfr9I CCCGGG 1 cut(s) 42
CseI GACGC 2 cut(s) 115, 155
CviJI RGCY 3 cut(s) 69, 176, 253
CviKI_1 RGCY 3 cut(s) 69, 176, 253
DdeI CTNAG 1 cut(s) 249
DpnI GATC 2 cut(s) 204, 219
DpnII GATC 2 cut(s) 202, 217
EciI GGCGGA 1 cut(s) 82
Eco47III AGCGCT 1 cut(s) 116
Eco88I CYCGRG 2 cut(s) 42, 110
Esp3I CGTCTC 2 cut(s) 41, 91
FaiI YATR 1 cut(s) 35
Fnu4HI GCNGC 1 cut(s) 177
Fsp4HI GCNGC 1 cut(s) 177
GlaI GCGC 2 cut(s) 79, 116
GluI GCNGC 1 cut(s) 177
GsaI CCCAGC 1 cut(s) 73
HaeII RGCGCY 2 cut(s) 81, 118
HaeIII GGCC 1 cut(s) 176
HapII CCGG 2 cut(s) 43, 66
HgaI GACGC 2 cut(s) 115, 155
HhaI GCGC 2 cut(s) 80, 117
Hin1I GRCGYC 2 cut(s) 147, 243
Hin6I GCGC 2 cut(s) 78, 115
HinP1I GCGC 2 cut(s) 78, 115
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 1 cut(s) 56
HpaII CCGG 2 cut(s) 43, 66
HphI GGTGA 2 cut(s) 190, 199
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 263
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 6 cut(s) 137, 155, 164, 173, 245, 248
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4IV ACGT 2 cut(s) 132, 243
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 123
HpyF3I CTNAG 1 cut(s) 249
HpySE526I ACGT 2 cut(s) 132, 243
Hsp92I GRCGYC 2 cut(s) 147, 243
HspAI GCGC 2 cut(s) 78, 115
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 2 cut(s) 202, 217
LmnI GCTCC 1 cut(s) 258
LpnPI CCDG 3 cut(s) 55, 56, 79
MaeII ACGT 2 cut(s) 132, 243
MalI GATC 2 cut(s) 204, 219
MboI GATC 2 cut(s) 202, 217
MboII GAAGA 2 cut(s) 17, 91
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 6 cut(s) 100, 115, 119, 148, 166, 198
MroNI GCCGGC 1 cut(s) 65
MspI CCGG 2 cut(s) 43, 66
MspR9I CCNGG 2 cut(s) 43, 44
MwoI GCNNNNNNNGC 2 cut(s) 75, 123
NaeI GCCGGC 1 cut(s) 67
NciI CCSGG 2 cut(s) 43, 44
NdeII GATC 2 cut(s) 202, 217
NgoMIV GCCGGC 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 110
PcsI WCGNNNNNNNCGW 5 cut(s) 159, 165, 168, 198, 240
PdiI GCCGGC 1 cut(s) 67
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 69
PspXI VCTCGAGB 1 cut(s) 110
PsyI GACNNNGTC 1 cut(s) 59
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 2 cut(s) 202, 217
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 2 cut(s) 43, 44
SetI ASST 3 cut(s) 135, 246, 255
Sfr274I CTCGAG 1 cut(s) 110
SlaI CTCGAG 1 cut(s) 110
SmaI CCCGGG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SsiI CCGC 3 cut(s) 93, 177, 184
StyD4I CCNGG 2 cut(s) 41, 42
TaaI ACNGT 1 cut(s) 85
TaiI ACGT 2 cut(s) 135, 246
TaqI TCGA 1 cut(s) 111
TaqII GACCGA 1 cut(s) 49
TauI GCSGC 1 cut(s) 179
TspGWI ACGGA 1 cut(s) 158
TspMI CCCGGG 1 cut(s) 42
Tth111I GACNNNGTC 1 cut(s) 59
XhoI CTCGAG 1 cut(s) 110
XmaI CCCGGG 1 cut(s) 42
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.