Rroxscaffold_3G00236320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005250
Physical Location & Seq
Forward (+)
23513003 .. 23513957
955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00236320.1

Sequence Viewer

Length: 534 bp
ATGTCGTCATTCCCCGTCATCGAGCACACCGTCCTCTTCAAGGTCAAAGACAACACCAATCCCTCCAAGGTCAACGGCCTCACCTCCCTCGGCCTCACCCCACCTCTCCGTTGGTCCCCTCCTCTCGCACCCGATCCACCCTCTTCACCTTCAATCACCGAGTCAACGCCGCAGCTGATCTCCGAGTCACCGCGGATCTCCATGCATGATCTGGTTTTGTTCCTCTACATCGTCGTTGCGGATGTTTGGCCCTCCACCTCGCCTTTCGACCGGATATTTGTCGGCTTTGTCTCCTTTGTCGGCGCCACCGTCTCCTCCGCCTTCTTCTCTTCCCTTGAGCACTTCTCCTACGTCCACGTTGCCACCACGGACGCCGACGAGGCCGCCCACCGCCCTCTCGCTCTCACCAATCTCACCCGCTCCGATCAACAACACCGGCCACCACGCAAACGCAAACGACGTCGCTCGCTCCCCATGATTCTCGGTCATGGATATGCACATTCTCGGTCCATCGAGAAAGATGCCGGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.53

Weight (kDa)

9.45

Isoelectric Point (pI)

70.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 302
AccBSI CCGCTC 1 cut(s) 420
AccII CGCG 1 cut(s) 193
AciI CCGC 8 cut(s) 170, 191, 193, 239, 318, 384, 391, 418
AclWI GGATC 2 cut(s) 128, 203
AcoI YGGCCR 1 cut(s) 437
AcyI GRCGYC 3 cut(s) 303, 372, 460
AfiI CCNNNNNNNGG 1 cut(s) 40
AgsI TTSAA 2 cut(s) 40, 153
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
Alw21I GWGCWC 2 cut(s) 27, 342
Alw26I GTCTC 2 cut(s) 295, 316
AlwI GGATC 2 cut(s) 128, 203
AoxI GGCC 5 cut(s) 76, 91, 248, 381, 437
ApeKI GCWGC 1 cut(s) 172
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 3 cut(s) 114, 249, 507
AsuHPI GGTGA 7 cut(s) 73, 88, 138, 148, 180, 397, 406
AvaII GGWCC 2 cut(s) 114, 507
BanI GGYRCC 1 cut(s) 302
Bbv12I GWGCWC 2 cut(s) 27, 342
BbvI GCAGC 1 cut(s) 184
BccI CCATC 1 cut(s) 518
BceAI ACGGC 1 cut(s) 91
BcgI CGANNNNNNTGC 1 cut(s) 503
BcoDI GTCTC 2 cut(s) 295, 316
BfoI RGCGCY 1 cut(s) 306
BglI GCCNNNNNGGC 1 cut(s) 380
BisI GCNGC 3 cut(s) 170, 173, 384
BlsI GCNGC 3 cut(s) 171, 174, 385
Bme18I GGWCC 2 cut(s) 114, 507
BmgT120I GGNCC 3 cut(s) 114, 249, 507
BmiI GGNNCC 2 cut(s) 116, 304
BmsI GCATC 1 cut(s) 511
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpuEI CTTGAG 1 cut(s) 356
BsaHI GRCGYC 3 cut(s) 303, 372, 460
BsaJI CCNNGG 4 cut(s) 66, 88, 191, 366
BsaWI WCCGGW 1 cut(s) 270
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse118I RCCGGY 1 cut(s) 435
BseDI CCNNGG 4 cut(s) 66, 88, 191, 366
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 40
BseRI GAGGAG 2 cut(s) 111, 304
BseXI GCAGC 1 cut(s) 184
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 5 cut(s) 78, 93, 250, 383, 439
BshNI GGYRCC 1 cut(s) 302
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 2 cut(s) 27, 342
BsiSI CCGG 3 cut(s) 271, 436, 525
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 40
BsmAI GTCTC 2 cut(s) 295, 316
BsmBI CGTCTC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 5 cut(s) 78, 93, 250, 383, 439
Bsp1286I GDGCHC 2 cut(s) 27, 342
Bsp143I GATC 5 cut(s) 133, 177, 195, 208, 424
BspACI CCGC 8 cut(s) 170, 191, 193, 239, 318, 384, 391, 418
BspANI GGCC 5 cut(s) 78, 93, 250, 383, 439
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 2 cut(s) 116, 304
BspPI GGATC 2 cut(s) 128, 203
BspT107I GGYRCC 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 420
BsrFI RCCGGY 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 435
BssECI CCNNGG 4 cut(s) 66, 88, 191, 366
BssMI GATC 5 cut(s) 133, 177, 195, 208, 424
BssNI GRCGYC 3 cut(s) 303, 372, 460
BssT1I CCWWGG 1 cut(s) 66
Bst4CI ACNGT 2 cut(s) 31, 310
Bst6I CTCTTC 3 cut(s) 41, 148, 334
BstACI GRCGYC 3 cut(s) 303, 372, 460
BstC8I GCNNGC 1 cut(s) 467
BstDSI CCRYGG 2 cut(s) 191, 366
BstENI CCTNNNNNAGG 1 cut(s) 38
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 193
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 5 cut(s) 136, 180, 198, 211, 427
BstMAI GTCTC 2 cut(s) 295, 316
BstMBI GATC 5 cut(s) 133, 177, 195, 208, 424
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
BsuRI GGCC 5 cut(s) 78, 93, 250, 383, 439
BtgI CCRYGG 2 cut(s) 191, 366
BtsCI GGATG 1 cut(s) 247
Cac8I GCNNGC 1 cut(s) 467
CfoI GCGC 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 435
Cfr13I GGNCC 3 cut(s) 114, 249, 507
Cfr42I CCGCGG 1 cut(s) 194
CseI GACGC 1 cut(s) 380
CviAII CATG 4 cut(s) 202, 206, 475, 488
CviJI RGCY 7 cut(s) 78, 93, 175, 250, 285, 383, 439
CviKI_1 RGCY 7 cut(s) 78, 93, 175, 250, 285, 383, 439
DinI GGCGCC 1 cut(s) 304
DpnI GATC 5 cut(s) 135, 179, 197, 210, 426
DpnII GATC 5 cut(s) 133, 177, 195, 208, 424
EaeI YGGCCR 1 cut(s) 437
Eam1104I CTCTTC 3 cut(s) 41, 148, 334
EarI CTCTTC 3 cut(s) 41, 148, 334
EciI GGCGGA 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 66
Eco47I GGWCC 2 cut(s) 114, 507
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 207
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 316
FaeI CATG 4 cut(s) 205, 209, 478, 491
FaiI YATR 5 cut(s) 203, 207, 476, 489, 495
FaqI GGGAC 1 cut(s) 100
FatI CATG 4 cut(s) 201, 205, 474, 487
FauI CCCGC 1 cut(s) 425
Fnu4HI GCNGC 3 cut(s) 170, 173, 384
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 3 cut(s) 170, 173, 384
GlaI GCGC 1 cut(s) 304
GluI GCNGC 3 cut(s) 170, 173, 384
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 5 cut(s) 78, 93, 250, 383, 439
HapII CCGG 3 cut(s) 271, 436, 525
HgaI GACGC 1 cut(s) 380
HhaI GCGC 1 cut(s) 305
Hin1I GRCGYC 3 cut(s) 303, 372, 460
Hin1II CATG 4 cut(s) 205, 209, 478, 491
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 2 cut(s) 73, 165
HindII GTYRAC 2 cut(s) 73, 165
HinfI GANTC 3 cut(s) 161, 185, 478
HpaII CCGG 3 cut(s) 271, 436, 525
HphI GGTGA 7 cut(s) 73, 88, 138, 148, 180, 397, 406
Hpy166II GTNNAC 3 cut(s) 73, 165, 355
Hpy188I TCNGA 2 cut(s) 184, 424
Hpy188III TCNNGA 1 cut(s) 514
Hpy8I GTNNAC 3 cut(s) 73, 165, 355
Hpy99I CGWCG 4 cut(s) 236, 380, 462, 465
HpyAV CCTTC 2 cut(s) 159, 331
HpyCH4III ACNGT 2 cut(s) 31, 310
HpyCH4IV ACGT 3 cut(s) 351, 357, 460
HpyCH4V TGCA 2 cut(s) 205, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpySE526I ACGT 3 cut(s) 351, 357, 460
Hsp92I GRCGYC 3 cut(s) 303, 372, 460
Hsp92II CATG 4 cut(s) 205, 209, 478, 491
HspAI GCGC 1 cut(s) 303
KasI GGCGCC 1 cut(s) 302
KspI CCGCGG 1 cut(s) 194
Kzo9I GATC 5 cut(s) 133, 177, 195, 208, 424
LmnI GCTCC 2 cut(s) 425, 474
LpnPI CCDG 3 cut(s) 197, 284, 449
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 1 cut(s) 511
MaeII ACGT 3 cut(s) 351, 357, 460
MaeIII GTNAC 1 cut(s) 186
MalI GATC 5 cut(s) 135, 179, 197, 210, 426
MbiI CCGCTC 1 cut(s) 420
MboI GATC 5 cut(s) 133, 177, 195, 208, 424
MboII GAAGA 4 cut(s) 28, 135, 316, 321
MflI RGATCY 1 cut(s) 195
MhlI GDGCHC 2 cut(s) 27, 342
Mly113I GGCGCC 1 cut(s) 303
MlyI GAGTC 2 cut(s) 170, 194
Mph1103I ATGCAT 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 492
MspA1I CMGCKG 2 cut(s) 175, 193
MspI CCGG 3 cut(s) 271, 436, 525
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 380
NarI GGCGCC 1 cut(s) 303
NdeII GATC 5 cut(s) 133, 177, 195, 208, 424
NlaIII CATG 4 cut(s) 205, 209, 478, 491
NlaIV GGNNCC 2 cut(s) 116, 304
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 186
NsiI ATGCAT 1 cut(s) 207
PcsI WCGNNNNNNNCGW 3 cut(s) 27, 306, 457
PfeI GAWTC 1 cut(s) 478
PkrI GCNGC 3 cut(s) 171, 174, 385
PleI GAGTC 2 cut(s) 169, 193
PluTI GGCGCC 1 cut(s) 306
PpsI GAGTC 2 cut(s) 169, 193
PspN4I GGNNCC 2 cut(s) 116, 304
PspPI GGNCC 3 cut(s) 114, 249, 507
PsuI RGATCY 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 175
RseI CAYNNNNRTG 1 cut(s) 492
SacII CCGCGG 1 cut(s) 194
SatI GCNGC 3 cut(s) 170, 173, 384
Sau3AI GATC 5 cut(s) 133, 177, 195, 208, 424
Sau96I GGNCC 3 cut(s) 114, 249, 507
SchI GAGTC 2 cut(s) 170, 194
SduI GDGCHC 2 cut(s) 27, 342
SfaNI GCATC 1 cut(s) 511
SfoI GGCGCC 1 cut(s) 304
Sfr303I CCGCGG 1 cut(s) 194
SgrBI CCGCGG 1 cut(s) 194
SinI GGWCC 2 cut(s) 114, 507
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
SsiI CCGC 8 cut(s) 170, 191, 193, 239, 318, 384, 391, 418
SspDI GGCGCC 1 cut(s) 302
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 2 cut(s) 31, 310
TaiI ACGT 3 cut(s) 354, 360, 463
TaqI TCGA 3 cut(s) 21, 267, 513
TaqII GACCGA 2 cut(s) 473, 495
TauI GCSGC 2 cut(s) 172, 386
TfiI GAWTC 1 cut(s) 478
TseFI GTSAC 1 cut(s) 186
TseI GCWGC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 186
TspGWI ACGGA 2 cut(s) 98, 383
VpaK11BI GGWCC 2 cut(s) 114, 507
XagI CCTNNNNNAGG 1 cut(s) 38
XcmI CCANNNNNNNNNTGG 2 cut(s) 108, 208
ZraI GACGTC 1 cut(s) 461
Zsp2I ATGCAT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.