MD14G1244700.v1.1

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002133
Physical Location & Seq
Reverse (-)
32300404 .. 32306576
6173 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1244700.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGAATCGGAAACCCACATGCCTCTTATCCGTCCGCGACGATATAGAAGAGAGGATGAAGATGCTGGCATCTCACACAGCTCAACAAGACACTGGAGACACCTTCGCTGAACGAGCCGACGCTTACTACAAACGACGCCCTCAGCTGCTCTCTCTGCTTCAAGACTTATACAAAGCCTACGTCACTTTATCTGACCGTTATATTCATACAATTGCCAAAACCAATCATCGCCGTGGACACTCCTCCCAAACCTCAATCATTGATAATGACTGCTACGACGACTGTCATGACCAACAAGATGCCGATGGTGATACATTAAGCCAGATGGATTCGGACGTGGGCAGTTCCCTCTCCTATCAGCCACAATTATCTCTTGCTGCTGCCCGGGATGATAACATGGTGTTGGATTTGGATGCAATTGTTGCGGAGGTTGTGATGAAGAATGTGGAATGCGATATCTTACTTGATGAGGCCAGCACCATGGGGAGGCGATGCAATGAGTCATCAAGGAAGATGAAGTTGCAGAAGAGCTTGCTGGAGGTGCTGGAGTCCGAAAGGCTTATACTACTGAATGAGAATGCTAAGTTGGGGTACAAAGTGGGTGCATTGGTGGAAGAGAACAAAGCCATCGCGTCCGAGTCCATGTTCATTAGGAGGAAGGCTGGAGAGCTGGCTAGGTGTTTGCTGAAGGTGAGGGAGGATCAGAGGGTGTGCATGCTTAGCCGTAAAATCGAGGACCTGCAGGGCCAGATCTATGGGTTGGAGAAGAGGAACAAGGAGTACTATGAGCAACTTGTGAAGAGAGACCAATGGTCCTCTCAACTTGTACAAGAAGAGAGTAGGAGCATAAACAACAAAAGCAAGTATAACGGGAATGAAGTGAATTTGGAGGTTTGTTTCCAAATAGGGAAGATCAATCGGAGGCTGAAGAGAGGTAATAGCAATGTGAAGGATTTTGGCACTGCGAGTGGGAAGAAGGGTTGGAGTTGGTGGGGAAAGGTCAAGAACATGGACTTGTTTCTATGCGGACATAATTCAAGCTCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIP1

Protein Analysis

350

Amino Acids

39.9

Weight (kDa)

7.53

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 12 - 67 1.7e-09 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 747
AccII CGCG 2 cut(s) 36, 632
AciI CCGC 3 cut(s) 34, 425, 1026
AclWI GGATC 1 cut(s) 708
AcsI RAATTY 1 cut(s) 883
AcuI CTGAAG 2 cut(s) 707, 947
AcyI GRCGYC 1 cut(s) 136
AfaI GTAC 3 cut(s) 593, 782, 828
AfiI CCNNNNNNNGG 1 cut(s) 486
AgsI TTSAA 2 cut(s) 161, 1038
AhdI GACNNNNNGTC 1 cut(s) 811
AjiI CACGTC 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 5 cut(s) 80, 145, 531, 670, 1041
AluI AGCT 5 cut(s) 80, 145, 531, 670, 1041
Alw26I GTCTC 2 cut(s) 90, 798
AlwI GGATC 1 cut(s) 708
Ama87I CYCGRG 1 cut(s) 384
AoxI GGCC 2 cut(s) 471, 745
ApeKI GCWGC 3 cut(s) 145, 377, 380
ApoI RAATTY 1 cut(s) 883
ArsI GACNNNNNNTTYG 2 cut(s) 165, 197
AspS9I GGNCC 3 cut(s) 736, 745, 813
AsuC2I CCSGG 2 cut(s) 385, 386
AsuHPI GGTGA 2 cut(s) 320, 703
AvaI CYCGRG 1 cut(s) 384
AvaII GGWCC 2 cut(s) 736, 813
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 132, 364, 367
BccI CCATC 3 cut(s) 299, 319, 635
BceAI ACGGC 2 cut(s) 216, 708
BcnI CCSGG 2 cut(s) 385, 386
BcoDI GTCTC 2 cut(s) 90, 798
BfaI CTAG 1 cut(s) 675
BfmI CTRYAG 1 cut(s) 740
BfuAI ACCTGC 1 cut(s) 747
BglII AGATCT 1 cut(s) 750
BisI GCNGC 3 cut(s) 146, 378, 381
BlpI GCTNAGC 1 cut(s) 719
BlsI GCNGC 3 cut(s) 147, 379, 382
BmcAI AGTACT 1 cut(s) 782
Bme1390I CCNGG 2 cut(s) 385, 386
Bme18I GGWCC 2 cut(s) 736, 813
BmeRI GACNNNNNGTC 1 cut(s) 811
BmeT110I CYCGRG 1 cut(s) 384
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 3 cut(s) 736, 745, 813
BmrFI CCNGG 2 cut(s) 385, 386
BmsI GCATC 5 cut(s) 51, 77, 289, 403, 482
BoxI GACNNNNGTC 1 cut(s) 282
BpmI CTGGAG 4 cut(s) 114, 557, 566, 684
Bpu10I CCTNAGC 1 cut(s) 141
Bpu1102I GCTNAGC 1 cut(s) 719
BpuMI CCSGG 2 cut(s) 385, 386
BsaHI GRCGYC 1 cut(s) 136
BsaI GGTCTC 1 cut(s) 798
BsaJI CCNNGG 3 cut(s) 232, 384, 480
Bsc4I CCNNNNNNNGG 1 cut(s) 486
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 2 cut(s) 502, 949
BseDI CCNNGG 3 cut(s) 232, 384, 480
BseGI GGATG 3 cut(s) 60, 394, 418
BseLI CCNNNNNNNGG 1 cut(s) 486
BseMI GCAATG 2 cut(s) 502, 949
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 232
BseXI GCAGC 3 cut(s) 132, 364, 367
Bsh1236I CGCG 2 cut(s) 36, 632
BshFI GGCC 2 cut(s) 473, 747
BsiHKCI CYCGRG 1 cut(s) 384
BsiSI CCGG 1 cut(s) 385
BslI CCNNNNNNNGG 1 cut(s) 486
BsmAI GTCTC 2 cut(s) 90, 798
BsmI GAATGC 2 cut(s) 455, 583
BsnI GGCC 2 cut(s) 473, 747
Bso31I GGTCTC 1 cut(s) 798
BsoBI CYCGRG 1 cut(s) 384
Bsp1407I TGTACA 1 cut(s) 826
Bsp143I GATC 3 cut(s) 700, 750, 912
Bsp1720I GCTNAGC 1 cut(s) 719
Bsp19I CCATGG 1 cut(s) 480
BspACI CCGC 3 cut(s) 34, 425, 1026
BspANI GGCC 2 cut(s) 473, 747
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 2 cut(s) 36, 632
BspHI TCATGA 1 cut(s) 286
BspMAI CTGCAG 1 cut(s) 744
BspMI ACCTGC 1 cut(s) 747
BspPI GGATC 1 cut(s) 708
BspQI GCTCTTC 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 798
BsrDI GCAATG 2 cut(s) 502, 949
BsrGI TGTACA 1 cut(s) 826
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 3 cut(s) 232, 384, 480
BssMI GATC 3 cut(s) 700, 750, 912
BssNI GRCGYC 1 cut(s) 136
BssT1I CCWWGG 1 cut(s) 480
Bst4CI ACNGT 2 cut(s) 197, 284
Bst6I CTCTTC 7 cut(s) 42, 521, 609, 761, 794, 828, 923
BstACI GRCGYC 1 cut(s) 136
BstAPI GCANNNNNTGC 1 cut(s) 422
BstAUI TGTACA 1 cut(s) 826
BstC8I GCNNGC 5 cut(s) 66, 475, 533, 672, 716
BstDEI CTNAG 3 cut(s) 141, 582, 719
BstDSI CCRYGG 2 cut(s) 232, 480
BstF5I GGATG 3 cut(s) 60, 394, 418
BstFNI CGCG 2 cut(s) 36, 632
BstKTI GATC 3 cut(s) 703, 753, 915
BstMAI GTCTC 2 cut(s) 90, 798
BstMBI GATC 3 cut(s) 700, 750, 912
BstMWI GCNNNNNNNGC 5 cut(s) 113, 154, 422, 541, 720
BstNSI RCATGY 2 cut(s) 21, 718
BstPAI GACNNNNGTC 1 cut(s) 282
BstSCI CCNGG 2 cut(s) 383, 384
BstSFI CTRYAG 1 cut(s) 740
BstUI CGCG 2 cut(s) 36, 632
BstV1I GCAGC 3 cut(s) 132, 364, 367
BstX2I RGATCY 1 cut(s) 750
BstXI CCANNNNNNTGG 2 cut(s) 481, 755
BstYI RGATCY 1 cut(s) 750
BsuRI GGCC 2 cut(s) 473, 747
BtgI CCRYGG 2 cut(s) 232, 480
BtgZI GCGATG 3 cut(s) 212, 505, 613
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 3 cut(s) 60, 394, 418
BtsI GCAGTG 1 cut(s) 960
BtsIMutI CAGTG 2 cut(s) 90, 960
BveI ACCTGC 1 cut(s) 747
Cac8I GCNNGC 5 cut(s) 66, 475, 533, 672, 716
CciI TCATGA 1 cut(s) 286
Cfr13I GGNCC 3 cut(s) 736, 745, 813
Cfr9I CCCGGG 1 cut(s) 384
CseI GACGC 3 cut(s) 128, 144, 621
Csp6I GTAC 3 cut(s) 592, 781, 827
CviAII CATG 7 cut(s) 18, 287, 397, 481, 643, 715, 1009
CviQI GTAC 3 cut(s) 592, 781, 827
DdeI CTNAG 3 cut(s) 141, 582, 719
DpnI GATC 3 cut(s) 702, 752, 914
DpnII GATC 3 cut(s) 700, 750, 912
DriI GACNNNNNGTC 1 cut(s) 811
Eam1104I CTCTTC 7 cut(s) 42, 521, 609, 761, 794, 828, 923
Eam1105I GACNNNNNGTC 1 cut(s) 811
EarI CTCTTC 7 cut(s) 42, 521, 609, 761, 794, 828, 923
Eco130I CCWWGG 1 cut(s) 480
Eco31I GGTCTC 1 cut(s) 798
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 736, 813
Eco57I CTGAAG 2 cut(s) 707, 947
Eco88I CYCGRG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 736
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 480
ErhI CCWWGG 1 cut(s) 480
FaeI CATG 7 cut(s) 21, 290, 400, 484, 646, 718, 1012
FalI AAGNNNNNCTT 1 cut(s) 1030
FatI CATG 7 cut(s) 17, 286, 396, 480, 642, 714, 1008
Fnu4HI GCNGC 3 cut(s) 146, 378, 381
FokI GGATG 3 cut(s) 67, 401, 425
Fsp4HI GCNGC 3 cut(s) 146, 378, 381
FspBI CTAG 1 cut(s) 675
GluI GCNGC 3 cut(s) 146, 378, 381
GsuI CTGGAG 4 cut(s) 114, 557, 566, 684
HaeIII GGCC 2 cut(s) 473, 747
HapII CCGG 1 cut(s) 385
HgaI GACGC 3 cut(s) 128, 144, 621
Hin1I GRCGYC 1 cut(s) 136
Hin1II CATG 7 cut(s) 21, 290, 400, 484, 646, 718, 1012
HinfI GANTC 5 cut(s) 4, 329, 500, 548, 638
HpaII CCGG 1 cut(s) 385
HphI GGTGA 2 cut(s) 320, 703
Hpy166II GTNNAC 1 cut(s) 236
Hpy188I TCNGA 7 cut(s) 9, 193, 334, 553, 637, 705, 921
Hpy188III TCNNGA 3 cut(s) 161, 287, 1003
Hpy8I GTNNAC 1 cut(s) 236
Hpy99I CGWCG 4 cut(s) 41, 122, 138, 281
HpyAV CCTTC 5 cut(s) 112, 652, 682, 943, 970
HpyCH4III ACNGT 2 cut(s) 197, 284
HpyCH4IV ACGT 2 cut(s) 180, 336
HpyCH4V TGCA 6 cut(s) 416, 495, 523, 605, 714, 742
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 154, 422, 541, 720
HpyF3I CTNAG 3 cut(s) 141, 582, 719
HpySE526I ACGT 2 cut(s) 180, 336
Hsp92I GRCGYC 1 cut(s) 136
Hsp92II CATG 7 cut(s) 21, 290, 400, 484, 646, 718, 1012
Kzo9I GATC 3 cut(s) 700, 750, 912
LguI GCTCTTC 1 cut(s) 521
LmnI GCTCC 1 cut(s) 843
Lsp1109I GCAGC 3 cut(s) 132, 364, 367
LweI GCATC 5 cut(s) 51, 77, 289, 403, 482
MaeI CTAG 1 cut(s) 675
MaeII ACGT 2 cut(s) 180, 336
MaeIII GTNAC 1 cut(s) 181
MalI GATC 3 cut(s) 702, 752, 914
MboI GATC 3 cut(s) 700, 750, 912
MfeI CAATTG 2 cut(s) 210, 417
MflI RGATCY 1 cut(s) 750
MluCI AATT 5 cut(s) 210, 365, 417, 883, 1033
MlyI GAGTC 3 cut(s) 509, 557, 647
MmeI TCCRAC 3 cut(s) 384, 741, 962
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 1 cut(s) 385
MspR9I CCNGG 2 cut(s) 385, 386
MunI CAATTG 2 cut(s) 210, 417
Mva1269I GAATGC 2 cut(s) 455, 583
MvnI CGCG 2 cut(s) 36, 632
MwoI GCNNNNNNNGC 5 cut(s) 113, 154, 422, 541, 720
NciI CCSGG 2 cut(s) 385, 386
NcoI CCATGG 1 cut(s) 480
NdeII GATC 3 cut(s) 700, 750, 912
NlaIII CATG 7 cut(s) 21, 290, 400, 484, 646, 718, 1012
NmuCI GTSAC 1 cut(s) 181
NspI RCATGY 2 cut(s) 21, 718
PaeI GCATGC 1 cut(s) 718
PagI TCATGA 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 521
PctI GAATGC 2 cut(s) 455, 583
PfeI GAWTC 2 cut(s) 4, 329
PkrI GCNGC 3 cut(s) 147, 379, 382
PleI GAGTC 3 cut(s) 508, 556, 646
PpsI GAGTC 3 cut(s) 508, 556, 646
PpuMI RGGWCCY 1 cut(s) 736
PshAI GACNNNNGTC 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 736
PspPI GGNCC 3 cut(s) 736, 745, 813
PspPPI RGGWCCY 1 cut(s) 736
PsrI GAACNNNNNNTAC 2 cut(s) 764, 796
PstI CTGCAG 1 cut(s) 744
PsuI RGATCY 1 cut(s) 750
PvuII CAGCTG 1 cut(s) 145
RsaI GTAC 3 cut(s) 593, 782, 828
RsaNI GTAC 3 cut(s) 592, 781, 827
RseI CAYNNNNRTG 1 cut(s) 231
SapI GCTCTTC 1 cut(s) 521
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 3 cut(s) 146, 378, 381
Sau3AI GATC 3 cut(s) 700, 750, 912
Sau96I GGNCC 3 cut(s) 736, 745, 813
SbfI CCTGCAGG 1 cut(s) 744
ScaI AGTACT 1 cut(s) 782
SchI GAGTC 3 cut(s) 509, 557, 647
ScrFI CCNGG 2 cut(s) 385, 386
SdaI CCTGCAGG 1 cut(s) 744
SfaNI GCATC 5 cut(s) 51, 77, 289, 403, 482
SfcI CTRYAG 1 cut(s) 740
SinI GGWCC 2 cut(s) 736, 813
SmaI CCCGGG 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 231
SphI GCATGC 1 cut(s) 718
Sse8387I CCTGCAGG 1 cut(s) 744
Sse9I AATT 5 cut(s) 210, 365, 417, 883, 1033
SsiI CCGC 3 cut(s) 34, 425, 1026
SspMI CTAG 1 cut(s) 675
StyD4I CCNGG 2 cut(s) 383, 384
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 2 cut(s) 197, 284
TaiI ACGT 2 cut(s) 183, 339
TaqI TCGA 1 cut(s) 732
TasI AATT 5 cut(s) 210, 365, 417, 883, 1033
TatI WGTACW 2 cut(s) 780, 826
TfiI GAWTC 2 cut(s) 4, 329
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 2 cut(s) 97, 967
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 3 cut(s) 145, 377, 380
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 7 cut(s) 17, 71, 194, 452, 530, 637, 891
TspGWI ACGGA 1 cut(s) 19
TspMI CCCGGG 1 cut(s) 384
TspRI CASTG 2 cut(s) 97, 967
VpaK11BI GGWCC 2 cut(s) 736, 813
XapI RAATTY 1 cut(s) 883
XceI RCATGY 2 cut(s) 21, 718
XmaI CCCGGG 1 cut(s) 384
XspI CTAG 1 cut(s) 675
ZrmI AGTACT 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.