pycom06g21390

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002133
Physical Location & Seq
Reverse (-)
24054892 .. 24057354
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g21390.1

Sequence Viewer

Length: 1182 bp
ATGTCGATGGGACCGACAGCGGGGAAGAAGCAAATAACAATGTCATCAGCTTCATCAAATCCTCCTCCTCCTCCTCCTTCAGTTGAGTCGTCGGCTCCTCCAAGTCCAAGAAGCAGTTTAAGCAGCAGCAACAAGTCCACCATGAATCAGAAACCCCCATGGCTCTTGCCCGTCCTTGACGATATGGAAGAGAGGATGAAGATGCTGGCATCTAACACAGCTCAACAAGACACTGGAGACACCTTCGCTGAACGAGCTGAGGCTTACTACAAACGACGCCCTCAGCTTCTCTCTCTGCTTCAAGACTTGTACAATGCCTACCTCACTTTATCCGATCGTTACATTCAAACAATCGCCAAAACCAATCATCGACGCGGAAACTCCTCCCAAACCTCAATCACTGATAATGACTACTATGACGACTATCATGACCAACAAGACCGAGATGGTGATACATTAAGCCAGATGGATTCGTACGTGGACAGTTCCCTCTCCTATCAGCAATTACCTCTGGCTGCTGCCGCTCAGGATGATGGCATGGTTTTGGATTTGGACGCGATTGTTACGGAGATTGTGATGAAGAATGTCGAATGCGATATCTTACTTGATGAGGCCAACACCACAGAGAGGCGATACAATGAGTCATCCAGGAAGATAGAGTTGCAGAAGAGCTTGCTTGAGGTGCTGGAGTCCGAAAGGCTCATACTACTGCATGAGAATGCCAAGTTGGGGTACAAAATGGGTGCACTGATGGAAGAGAACAAAGCCCTCGCATCCGAGTCCATGTTCATCAAGAGGAAAGCTGGAGAGCTGGCTAGATGTCTGCTCAAGGTGAGGGAGGATCAGAGGGTGTGCATGCTTAGCCGTAAAATCGAGGACCTGCAGGGCCAGATTTATGGGTTGGAGAGGAGGAACAAGGAGTACTATGAGCAACTCGTGAAGAGAGACCAATGGTCCTCCCAACTTGTACAAGAAGAGAGTAGGAGCATCAACAACAAGAGCAAGTTTAATGGGAATGAGGTGAACATGGAGGTTTGCTTCCAAATAGGGAAGATCAATCGGAGGCTGAAGAGCGGTCATAGCACAGTGAAGGATTTTGGCGCTGTGAGCGGGAAGAAGGGTTTGAGTTGGTGGGGAAAGGTCAAGAACATGGATTTGTTTCTGTGCGGAGGCTCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIP1

Protein Analysis

394

Amino Acids

44.49

Weight (kDa)

5.98

Isoelectric Point (pI)

60.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 49 - 114 7.4e-13 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 888
AccBSI CCGCTC 3 cut(s) 524, 1074, 1110
AccII CGCG 2 cut(s) 375, 557
AciI CCGC 6 cut(s) 20, 375, 522, 1074, 1110, 1167
AclWI GGATC 1 cut(s) 849
AcuI CTGAAG 2 cut(s) 63, 1088
AcyI GRCGYC 1 cut(s) 277
AfaI GTAC 5 cut(s) 311, 476, 734, 923, 969
AfiI CCNNNNNNNGG 3 cut(s) 20, 627, 729
AgsI TTSAA 2 cut(s) 302, 347
AhdI GACNNNNNGTC 1 cut(s) 952
AjnI CCWGG 1 cut(s) 647
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AluBI AGCT 7 cut(s) 50, 221, 257, 286, 672, 803, 811
AluI AGCT 7 cut(s) 50, 221, 257, 286, 672, 803, 811
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 2 cut(s) 231, 939
Alw44I GTGCAC 1 cut(s) 744
AlwI GGATC 1 cut(s) 849
AoxI GGCC 2 cut(s) 612, 886
ApaLI GTGCAC 1 cut(s) 744
ApeKI GCWGC 4 cut(s) 123, 126, 515, 518
AspLEI GCGC 1 cut(s) 1103
AspS9I GGNCC 4 cut(s) 11, 877, 886, 954
AsuHPI GGTGA 3 cut(s) 461, 844, 1033
AvaII GGWCC 3 cut(s) 11, 877, 954
BaeGI GKGCMC 1 cut(s) 748
BauI CACGAG 1 cut(s) 935
Bbv12I GWGCWC 1 cut(s) 748
BbvCI CCTCAGC 2 cut(s) 258, 282
BbvI GCAGC 4 cut(s) 135, 138, 502, 505
BccI CCATC 4 cut(s) 440, 460, 527, 745
BceAI ACGGC 1 cut(s) 849
BciT130I CCWGG 1 cut(s) 649
BcoDI GTCTC 2 cut(s) 231, 939
BfaI CTAG 1 cut(s) 816
BfmI CTRYAG 1 cut(s) 881
BfoI RGCGCY 1 cut(s) 1104
BfuAI ACCTGC 1 cut(s) 888
BisI GCNGC 5 cut(s) 124, 127, 516, 519, 522
BlpI GCTNAGC 1 cut(s) 860
BlsI GCNGC 5 cut(s) 125, 128, 517, 520, 523
BmcAI AGTACT 1 cut(s) 923
Bme1390I CCNGG 1 cut(s) 649
Bme18I GGWCC 3 cut(s) 11, 877, 954
BmeRI GACNNNNNGTC 1 cut(s) 952
BmgT120I GGNCC 4 cut(s) 11, 877, 886, 954
BmiI GGNNCC 2 cut(s) 12, 96
BmrFI CCNGG 1 cut(s) 649
BmsI GCATC 4 cut(s) 192, 218, 782, 996
BpmI CTGGAG 3 cut(s) 255, 707, 825
Bpu10I CCTNAGC 3 cut(s) 258, 282, 525
Bpu1102I GCTNAGC 1 cut(s) 860
BpuEI CTTGAG 2 cut(s) 698, 812
BsaAI YACGTR 1 cut(s) 478
BsaHI GRCGYC 1 cut(s) 277
BsaI GGTCTC 1 cut(s) 939
BsaJI CCNNGG 1 cut(s) 158
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 627, 729
Bse1I ACTGG 1 cut(s) 238
BseBI CCWGG 1 cut(s) 649
BseDI CCNNGG 1 cut(s) 158
BseGI GGATG 4 cut(s) 201, 535, 644, 773
BseLI CCNNNNNNNGG 3 cut(s) 20, 627, 729
BseMII CTCAG 3 cut(s) 249, 296, 539
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 7 cut(s) 54, 57, 60, 63, 87, 373, 922
BseSI GKGCMC 1 cut(s) 748
BseXI GCAGC 4 cut(s) 135, 138, 502, 505
Bsh1236I CGCG 2 cut(s) 375, 557
Bsh1285I CGRYCG 1 cut(s) 337
BshFI GGCC 2 cut(s) 614, 888
BsiEI CGRYCG 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 748
BsiWI CGTACG 1 cut(s) 474
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 3 cut(s) 20, 627, 729
BsmAI GTCTC 2 cut(s) 231, 939
BsmFI GGGAC 1 cut(s) 24
BsmI GAATGC 2 cut(s) 596, 724
BsnI GGCC 2 cut(s) 614, 888
Bso31I GGTCTC 1 cut(s) 939
Bsp1286I GDGCHC 1 cut(s) 748
Bsp1407I TGTACA 2 cut(s) 309, 967
Bsp143I GATC 3 cut(s) 334, 841, 1053
Bsp1720I GCTNAGC 1 cut(s) 860
Bsp19I CCATGG 1 cut(s) 158
BspACI CCGC 6 cut(s) 20, 375, 522, 1074, 1110, 1167
BspANI GGCC 2 cut(s) 614, 888
BspCNI CTCAG 3 cut(s) 250, 295, 538
BspFNI CGCG 2 cut(s) 375, 557
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 2 cut(s) 12, 96
BspMAI CTGCAG 1 cut(s) 885
BspMI ACCTGC 1 cut(s) 888
BspPI GGATC 1 cut(s) 849
BspQI GCTCTTC 2 cut(s) 662, 1064
BspTNI GGTCTC 1 cut(s) 939
BsrBI CCGCTC 3 cut(s) 524, 1074, 1110
BsrGI TGTACA 2 cut(s) 309, 967
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 158
BssMI GATC 3 cut(s) 334, 841, 1053
BssNI GRCGYC 1 cut(s) 277
BssSI CACGAG 1 cut(s) 935
BssT1I CCWWGG 1 cut(s) 158
Bst2BI CACGAG 1 cut(s) 935
Bst2UI CCWGG 1 cut(s) 649
Bst4CI ACNGT 2 cut(s) 485, 1087
Bst6I CTCTTC 6 cut(s) 183, 662, 750, 935, 969, 1064
BstACI GRCGYC 1 cut(s) 277
BstAUI TGTACA 2 cut(s) 309, 967
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 4 cut(s) 207, 674, 813, 857
BstDEI CTNAG 4 cut(s) 258, 282, 525, 860
BstDSI CCRYGG 1 cut(s) 158
BstF5I GGATG 4 cut(s) 201, 535, 644, 773
BstFNI CGCG 2 cut(s) 375, 557
BstH2I RGCGCY 1 cut(s) 1104
BstHHI GCGC 1 cut(s) 1103
BstKTI GATC 3 cut(s) 337, 844, 1056
BstMAI GTCTC 2 cut(s) 231, 939
BstMBI GATC 3 cut(s) 334, 841, 1053
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 7 cut(s) 120, 254, 521, 682, 861, 1080, 1107
BstNI CCWGG 1 cut(s) 649
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 1 cut(s) 647
BstSFI CTRYAG 1 cut(s) 881
BstSLI GKGCMC 1 cut(s) 748
BstUI CGCG 2 cut(s) 375, 557
BstV1I GCAGC 4 cut(s) 135, 138, 502, 505
BstXI CCANNNNNNTGG 1 cut(s) 896
BsuRI GGCC 2 cut(s) 614, 888
BtgI CCRYGG 1 cut(s) 158
BtsCI GGATG 4 cut(s) 201, 535, 644, 773
BtsIMutI CAGTG 4 cut(s) 231, 399, 746, 1092
BveI ACCTGC 1 cut(s) 888
Cac8I GCNNGC 4 cut(s) 207, 674, 813, 857
CciI TCATGA 1 cut(s) 427
CfoI GCGC 1 cut(s) 1103
Cfr13I GGNCC 4 cut(s) 11, 877, 886, 954
CseI GACGC 3 cut(s) 285, 381, 563
Csp6I GTAC 5 cut(s) 310, 475, 733, 922, 968
CviAII CATG 9 cut(s) 142, 159, 428, 538, 713, 784, 856, 1027, 1150
CviQI GTAC 5 cut(s) 310, 475, 733, 922, 968
DdeI CTNAG 4 cut(s) 258, 282, 525, 860
DpnI GATC 3 cut(s) 336, 843, 1055
DpnII GATC 3 cut(s) 334, 841, 1053
DriI GACNNNNNGTC 1 cut(s) 952
Eam1104I CTCTTC 6 cut(s) 183, 662, 750, 935, 969, 1064
Eam1105I GACNNNNNGTC 1 cut(s) 952
EarI CTCTTC 6 cut(s) 183, 662, 750, 935, 969, 1064
Eco130I CCWWGG 1 cut(s) 158
Eco31I GGTCTC 1 cut(s) 939
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 3 cut(s) 11, 877, 954
Eco57I CTGAAG 2 cut(s) 63, 1088
EcoO109I RGGNCCY 1 cut(s) 877
EcoRII CCWGG 1 cut(s) 647
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 9 cut(s) 145, 162, 431, 541, 716, 787, 859, 1030, 1153
FaqI GGGAC 1 cut(s) 24
FatI CATG 9 cut(s) 141, 158, 427, 537, 712, 783, 855, 1026, 1149
FauI CCCGC 2 cut(s) 13, 1103
Fnu4HI GCNGC 5 cut(s) 124, 127, 516, 519, 522
FokI GGATG 4 cut(s) 208, 542, 631, 760
Fsp4HI GCNGC 5 cut(s) 124, 127, 516, 519, 522
FspBI CTAG 1 cut(s) 816
GlaI GCGC 1 cut(s) 1102
GluI GCNGC 5 cut(s) 124, 127, 516, 519, 522
GsuI CTGGAG 3 cut(s) 255, 707, 825
HaeII RGCGCY 1 cut(s) 1104
HaeIII GGCC 2 cut(s) 614, 888
HgaI GACGC 3 cut(s) 285, 381, 563
HhaI GCGC 1 cut(s) 1103
Hin1I GRCGYC 1 cut(s) 277
Hin1II CATG 9 cut(s) 145, 162, 431, 541, 716, 787, 859, 1030, 1153
Hin6I GCGC 1 cut(s) 1101
HinP1I GCGC 1 cut(s) 1101
HinfI GANTC 6 cut(s) 86, 145, 470, 641, 689, 779
HphI GGTGA 3 cut(s) 461, 844, 1033
Hpy166II GTNNAC 4 cut(s) 138, 481, 746, 1024
Hpy188I TCNGA 6 cut(s) 150, 334, 694, 778, 846, 1062
Hpy188III TCNNGA 6 cut(s) 302, 428, 527, 793, 937, 1144
Hpy8I GTNNAC 4 cut(s) 138, 481, 746, 1024
Hpy99I CGWCG 3 cut(s) 94, 279, 375
HpyAV CCTTC 4 cut(s) 87, 253, 1084, 1111
HpyCH4III ACNGT 2 cut(s) 485, 1087
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 5 cut(s) 664, 712, 746, 855, 883
HpyF10VI GCNNNNNNNGC 7 cut(s) 120, 254, 521, 682, 861, 1080, 1107
HpyF3I CTNAG 4 cut(s) 258, 282, 525, 860
HpySE526I ACGT 1 cut(s) 477
Hsp92I GRCGYC 1 cut(s) 277
Hsp92II CATG 9 cut(s) 145, 162, 431, 541, 716, 787, 859, 1030, 1153
HspAI GCGC 1 cut(s) 1101
Kzo9I GATC 3 cut(s) 334, 841, 1053
LguI GCTCTTC 2 cut(s) 662, 1064
LmnI GCTCC 2 cut(s) 100, 984
Lsp1109I GCAGC 4 cut(s) 135, 138, 502, 505
LweI GCATC 4 cut(s) 192, 218, 782, 996
MaeI CTAG 1 cut(s) 816
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 2 cut(s) 338, 562
MalI GATC 3 cut(s) 336, 843, 1055
MbiI CCGCTC 3 cut(s) 524, 1074, 1110
MboI GATC 3 cut(s) 334, 841, 1053
MhlI GDGCHC 1 cut(s) 748
MluCI AATT 1 cut(s) 503
MlyI GAGTC 4 cut(s) 95, 650, 698, 788
MmeI TCCRAC 1 cut(s) 882
MseI TTAA 4 cut(s) 119, 458, 1008, 1180
MslI CAYNNNNRTG 1 cut(s) 717
MspA1I CMGCKG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 649
Mva1269I GAATGC 2 cut(s) 596, 724
MvaI CCWGG 1 cut(s) 649
MvnI CGCG 2 cut(s) 375, 557
MwoI GCNNNNNNNGC 7 cut(s) 120, 254, 521, 682, 861, 1080, 1107
NcoI CCATGG 1 cut(s) 158
NdeII GATC 3 cut(s) 334, 841, 1053
NlaIII CATG 9 cut(s) 145, 162, 431, 541, 716, 787, 859, 1030, 1153
NlaIV GGNNCC 2 cut(s) 12, 96
NspI RCATGY 1 cut(s) 859
PaeI GCATGC 1 cut(s) 859
PagI TCATGA 1 cut(s) 427
PciSI GCTCTTC 2 cut(s) 662, 1064
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 2 cut(s) 596, 724
PfeI GAWTC 2 cut(s) 145, 470
Pfl23II CGTACG 1 cut(s) 474
PfoI TCCNGGA 1 cut(s) 647
PkrI GCNGC 5 cut(s) 125, 128, 517, 520, 523
Ple19I CGATCG 1 cut(s) 337
PleI GAGTC 4 cut(s) 94, 649, 697, 787
PpsI GAGTC 4 cut(s) 94, 649, 697, 787
Ppu21I YACGTR 1 cut(s) 478
PpuMI RGGWCCY 1 cut(s) 877
Psp5II RGGWCCY 1 cut(s) 877
Psp6I CCWGG 1 cut(s) 647
PspGI CCWGG 1 cut(s) 647
PspLI CGTACG 1 cut(s) 474
PspN4I GGNNCC 2 cut(s) 12, 96
PspPI GGNCC 4 cut(s) 11, 877, 886, 954
PspPPI RGGWCCY 1 cut(s) 877
PsrI GAACNNNNNNTAC 2 cut(s) 905, 937
PstI CTGCAG 1 cut(s) 885
PvuI CGATCG 1 cut(s) 337
RsaI GTAC 5 cut(s) 311, 476, 734, 923, 969
RsaNI GTAC 5 cut(s) 310, 475, 733, 922, 968
RseI CAYNNNNRTG 1 cut(s) 717
SapI GCTCTTC 2 cut(s) 662, 1064
SaqAI TTAA 4 cut(s) 119, 458, 1008, 1180
SatI GCNGC 5 cut(s) 124, 127, 516, 519, 522
Sau3AI GATC 3 cut(s) 334, 841, 1053
Sau96I GGNCC 4 cut(s) 11, 877, 886, 954
SbfI CCTGCAGG 1 cut(s) 885
ScaI AGTACT 1 cut(s) 923
SchI GAGTC 4 cut(s) 95, 650, 698, 788
ScrFI CCNGG 1 cut(s) 649
SdaI CCTGCAGG 1 cut(s) 885
SduI GDGCHC 1 cut(s) 748
SfaNI GCATC 4 cut(s) 192, 218, 782, 996
SfcI CTRYAG 1 cut(s) 881
SinI GGWCC 3 cut(s) 11, 877, 954
SmiMI CAYNNNNRTG 1 cut(s) 717
SmlI CTYRAG 2 cut(s) 677, 827
SmoI CTYRAG 2 cut(s) 677, 827
SphI GCATGC 1 cut(s) 859
Sse8387I CCTGCAGG 1 cut(s) 885
Sse9I AATT 1 cut(s) 503
SsiI CCGC 6 cut(s) 20, 375, 522, 1074, 1110, 1167
SspMI CTAG 1 cut(s) 816
StyD4I CCNGG 1 cut(s) 647
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 2 cut(s) 485, 1087
TaiI ACGT 1 cut(s) 480
TaqI TCGA 4 cut(s) 5, 370, 588, 873
TaqII GACCGA 2 cut(s) 28, 456
TasI AATT 1 cut(s) 503
TatI WGTACW 3 cut(s) 309, 921, 967
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 145, 470
Tru1I TTAA 4 cut(s) 119, 458, 1008, 1180
Tru9I TTAA 4 cut(s) 119, 458, 1008, 1180
TscAI CASTG 4 cut(s) 238, 406, 753, 1092
TseI GCWGC 4 cut(s) 123, 126, 515, 518
TspDTI ATGAA 5 cut(s) 42, 158, 212, 593, 778
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 4 cut(s) 238, 406, 753, 1092
VneI GTGCAC 1 cut(s) 744
VpaK11BI GGWCC 3 cut(s) 11, 877, 954
XceI RCATGY 1 cut(s) 859
XspI CTAG 1 cut(s) 816
ZrmI AGTACT 1 cut(s) 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.