MD15G1067500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003210
Physical Location & Seq
Reverse (-)
4691026 .. 4692529
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1067500.v1.1.491

Sequence Viewer

Length: 564 bp
ATGAATATATCACCATCACAATACAATAGTGGATGTGAATCTGGATGGACAAGTTACTTGGATCAGTCCTCTTATCTTTCTCAAAGTCAGTTCCAAAAACCTGGGGGTTTTGCTGATTATGTGGAGACAAAAGAAGAAGAAGAAGAAGAAGAAGAGGAAGAGGATCTTTCAATGGTTTCTGATGCTTCTTCTGGACCTCCACATTACCACGATTTTGACGAAGACTGCGTGTACGAAGACGGATCTTCTTTTTCGGTTTCGTGGGGTTCCAAATTGGGAAAGAAAAGCAAGAAGAAGAGCAAAGGAAATGGCAGCAGAGAGCTCCACCTTGATGACACCGCTAGCTCCCCAGTCCTCAACTATTCCAAGAAGAAGTTGAGTACTAATTTCTCAAAAGATGAATCTTCAATGCAGAATGTATTGGGTTACTCTGAGGGTTTTTCTGCAACACACAAGAAGGGAAAATCTGCATTGCTGCAGCACTTTGGTTTCTTGAAGTCATCTCTACCCAAAAGTCCAGCTTCACAAAAACCAGGTGATTTGCAAGGTGAAAGTTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

20.66

Weight (kDa)

4.94

Isoelectric Point (pI)

59.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 339
AclWI GGATC 3 cut(s) 69, 171, 250
AfaI GTAC 2 cut(s) 233, 382
AgsI TTSAA 3 cut(s) 171, 408, 496
AjnI CCWGG 2 cut(s) 100, 532
AluBI AGCT 3 cut(s) 322, 345, 521
AluI AGCT 3 cut(s) 322, 345, 521
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 3 cut(s) 69, 171, 250
ApeKI GCWGC 3 cut(s) 312, 475, 478
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 3 cut(s) 3, 548, 560
AsuNHI GCTAGC 1 cut(s) 341
AvaII GGWCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 324
BbsI GAAGAC 2 cut(s) 228, 243
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 3 cut(s) 324, 462, 490
BccI CCATC 2 cut(s) 22, 39
BciT130I CCWGG 2 cut(s) 102, 534
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 1 cut(s) 342
BfmI CTRYAG 1 cut(s) 476
BisI GCNGC 3 cut(s) 313, 476, 479
BlsI GCNGC 3 cut(s) 314, 477, 480
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 102, 534
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 2 cut(s) 102, 534
BmrI ACTGGG 1 cut(s) 344
BmsI GCATC 1 cut(s) 172
BmtI GCTAGC 1 cut(s) 345
BmuI ACTGGG 1 cut(s) 344
BpiI GAAGAC 2 cut(s) 228, 243
BsaBI GATNNNNATC 1 cut(s) 37
BsaJI CCNNGG 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 350
Bse3DI GCAATG 1 cut(s) 470
Bse8I GATNNNNATC 1 cut(s) 37
BseBI CCWGG 2 cut(s) 102, 534
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 2 cut(s) 38, 50
BseJI GATNNNNATC 1 cut(s) 37
BseMI GCAATG 1 cut(s) 470
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 350
BseXI GCAGC 3 cut(s) 324, 462, 490
BsiHKAI GWGCWC 1 cut(s) 324
BsmAI GTCTC 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 3 cut(s) 61, 163, 242
BspACI CCGC 1 cut(s) 339
BspCNI CTCAG 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 268
BspMAI CTGCAG 1 cut(s) 480
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 3 cut(s) 69, 171, 250
BspQI GCTCTTC 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 470
BsrI ACTGG 1 cut(s) 350
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 3 cut(s) 61, 163, 242
Bst2UI CCWGG 2 cut(s) 102, 534
Bst6I CTCTTC 3 cut(s) 147, 153, 290
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 1 cut(s) 432
BstF5I GGATG 2 cut(s) 38, 50
BstKTI GATC 3 cut(s) 64, 166, 245
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 3 cut(s) 61, 163, 242
BstNI CCWGG 2 cut(s) 102, 534
BstSCI CCNGG 2 cut(s) 100, 532
BstSFI CTRYAG 1 cut(s) 476
BstV1I GCAGC 3 cut(s) 324, 462, 490
BstV2I GAAGAC 2 cut(s) 228, 243
BstX2I RGATCY 2 cut(s) 163, 242
BstXI CCANNNNNNTGG 1 cut(s) 101
BstYI RGATCY 2 cut(s) 163, 242
BtsCI GGATG 2 cut(s) 38, 50
Cac8I GCNNGC 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 194
CsiI ACCWGGT 1 cut(s) 532
Csp6I GTAC 2 cut(s) 232, 381
CviJI RGCY 3 cut(s) 322, 345, 521
CviKI_1 RGCY 3 cut(s) 322, 345, 521
CviQI GTAC 2 cut(s) 232, 381
DdeI CTNAG 1 cut(s) 432
DpnI GATC 3 cut(s) 63, 165, 244
DpnII GATC 3 cut(s) 61, 163, 242
Eam1104I CTCTTC 3 cut(s) 147, 153, 290
EarI CTCTTC 3 cut(s) 147, 153, 290
Ecl136II GAGCTC 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 322
EcoICRI GAGCTC 1 cut(s) 322
EcoRII CCWGG 2 cut(s) 100, 532
EcoT38I GRGCYC 1 cut(s) 324
FaiI YATR 2 cut(s) 8, 120
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 505, 537
Fnu4HI GCNGC 3 cut(s) 313, 476, 479
FokI GGATG 2 cut(s) 45, 57
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 3 cut(s) 313, 476, 479
FspBI CTAG 1 cut(s) 342
GluI GCNGC 3 cut(s) 313, 476, 479
HinfI GANTC 2 cut(s) 38, 401
HphI GGTGA 3 cut(s) 3, 548, 560
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 2 cut(s) 181, 433
Hpy188III TCNNGA 3 cut(s) 42, 192, 493
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 1 cut(s) 451
HpyCH4V TGCA 5 cut(s) 412, 446, 470, 478, 544
HpyF3I CTNAG 1 cut(s) 432
Kzo9I GATC 3 cut(s) 61, 163, 242
LguI GCTCTTC 1 cut(s) 290
LmnI GCTCC 2 cut(s) 327, 350
LpnPI CCDG 8 cut(s) 27, 87, 114, 177, 363, 519, 531, 546
Lsp1109I GCAGC 3 cut(s) 324, 462, 490
LweI GCATC 1 cut(s) 172
MabI ACCWGGT 1 cut(s) 532
MaeI CTAG 1 cut(s) 342
MaeIII GTNAC 2 cut(s) 53, 425
MalI GATC 3 cut(s) 63, 165, 244
MboI GATC 3 cut(s) 61, 163, 242
MflI RGATCY 2 cut(s) 163, 242
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 2 cut(s) 272, 385
MnlI CCTC 6 cut(s) 79, 148, 154, 207, 365, 427
MslI CAYNNNNRTG 1 cut(s) 330
MspR9I CCNGG 2 cut(s) 102, 534
MvaI CCWGG 2 cut(s) 102, 534
NdeII GATC 3 cut(s) 61, 163, 242
NheI GCTAGC 1 cut(s) 341
NlaIV GGNNCC 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 290
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 225
PfeI GAWTC 2 cut(s) 38, 401
PkrI GCNGC 3 cut(s) 314, 477, 480
Psp124BI GAGCTC 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 100, 532
PspGI CCWGG 2 cut(s) 100, 532
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 1 cut(s) 194
PstI CTGCAG 1 cut(s) 480
PsuI RGATCY 2 cut(s) 163, 242
RsaI GTAC 2 cut(s) 233, 382
RsaNI GTAC 2 cut(s) 232, 381
RseI CAYNNNNRTG 1 cut(s) 330
SacI GAGCTC 1 cut(s) 324
SapI GCTCTTC 1 cut(s) 290
SatI GCNGC 3 cut(s) 313, 476, 479
Sau3AI GATC 3 cut(s) 61, 163, 242
Sau96I GGNCC 1 cut(s) 194
ScaI AGTACT 1 cut(s) 382
ScrFI CCNGG 2 cut(s) 102, 534
SduI GDGCHC 1 cut(s) 324
SetI ASST 8 cut(s) 103, 199, 324, 330, 347, 523, 538, 550
SexAI ACCWGGT 1 cut(s) 532
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 1 cut(s) 476
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 330
Sse9I AATT 2 cut(s) 272, 385
SsiI CCGC 1 cut(s) 339
SspMI CTAG 1 cut(s) 342
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 2 cut(s) 100, 532
TasI AATT 2 cut(s) 272, 385
TatI WGTACW 1 cut(s) 380
TfiI GAWTC 2 cut(s) 38, 401
TseI GCWGC 3 cut(s) 312, 475, 478
TspDTI ATGAA 2 cut(s) 17, 414
TspGWI ACGGA 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 194
XspI CTAG 1 cut(s) 342
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.