pycom10g09340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003210
Physical Location & Seq
Forward (+)
12343413 .. 12344393
981 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g09340.1

Sequence Viewer

Length: 624 bp
ATGAATGTGTTGGCCTCAGAATGCAGTAGTGGTTGTGAGTCTGGTTGGACTGTATACTTGGAGCACTCTAATTATCTTTCTCGTAATCCAAGTGCTTCACGCAGCAGAGATAATAATTGCAAGAGCAAGTCCGATTTTTGTGGTAAACAAGTTATTCATGATAAGGAGGAGGATGATGAAGAAGAAGATCAGTCAATGGTTTCTGATGCATCTTCTGGGCCTCCACATTTCAATGAAGATGAAGTTTACTTGGATGAAAACAACAGCATCAACAATAATTATGGGTCCTTTTATCCTCCTCAAATATCCAAGGATGCTGGAAATTTGAAGTTTGGAAGAAAAAGGCAGCAGAGAAATAATATCAAAGGAAAAGGTAGGCGCTCATGTGGTGATCAACAACAACAACAACAACCATCTTTATTAGATGACACTGCTAGCTCTCCTGTCTTCAACTACTCCAAGAACAACTTCATTGTGTCAAATAATCAAGCTTCTGGAGACAGTGTTCTGGATTATTCACAAGGTTTCTCATCTACACATTTTAAGGGGAGATCTGCATACCAAGACCACTGTGGTTTCTTACAGTCTACTCCATCTGGAAATAACCAGTGGTTTCAAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

23.14

Weight (kDa)

5.2

Isoelectric Point (pI)

61.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 54, 587
AcsI RAATTY 1 cut(s) 322
AgsI TTSAA 4 cut(s) 232, 328, 451, 617
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AloI GAACNNNNNNTCC 2 cut(s) 489, 521
AluBI AGCT 2 cut(s) 438, 491
AluI AGCT 2 cut(s) 438, 491
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 1 cut(s) 492
AoxI GGCC 2 cut(s) 12, 218
ApeKI GCWGC 2 cut(s) 102, 346
ApoI RAATTY 1 cut(s) 322
Asp700I GAANNNNTTC 1 cut(s) 467
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 2 cut(s) 218, 285
AsuHPI GGTGA 1 cut(s) 401
AsuNHI GCTAGC 1 cut(s) 434
AvaII GGWCC 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 439
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 114, 358
BccI CCATC 2 cut(s) 421, 601
BclI TGATCA 1 cut(s) 391
BcoDI GTCTC 1 cut(s) 492
BfaI CTAG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 382
BglII AGATCT 1 cut(s) 551
BisI GCNGC 2 cut(s) 103, 347
BlsI GCNGC 2 cut(s) 104, 348
Bme18I GGWCC 1 cut(s) 285
BmgT120I GGNCC 2 cut(s) 218, 285
BmiI GGNNCC 1 cut(s) 286
BmsI GCATC 4 cut(s) 196, 218, 276, 304
BmtI GCTAGC 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 439
BpmI CTGGAG 1 cut(s) 516
BsaJI CCNNGG 1 cut(s) 309
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bse1I ACTGG 1 cut(s) 607
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 3 cut(s) 178, 259, 319
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 1 cut(s) 607
BseRI GAGGAG 2 cut(s) 182, 288
BseXI GCAGC 2 cut(s) 114, 358
BshFI GGCC 2 cut(s) 14, 220
BsiHKAI GWGCWC 1 cut(s) 66
BsmAI GTCTC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 2 cut(s) 14, 220
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 3 cut(s) 187, 391, 551
BspANI GGCC 2 cut(s) 14, 220
BspCNI CTCAG 1 cut(s) 29
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 286
BspOI GCTAGC 1 cut(s) 438
BsrI ACTGG 1 cut(s) 607
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 3 cut(s) 187, 391, 551
BssNAI GTATAC 1 cut(s) 55
BssT1I CCWWGG 1 cut(s) 309
Bst1107I GTATAC 1 cut(s) 55
Bst4CI ACNGT 4 cut(s) 52, 503, 572, 585
BstC8I GCNNGC 1 cut(s) 436
BstDEI CTNAG 1 cut(s) 16
BstF5I GGATG 3 cut(s) 178, 259, 319
BstH2I RGCGCY 1 cut(s) 382
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 3 cut(s) 190, 394, 554
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 3 cut(s) 187, 391, 551
BstV1I GCAGC 2 cut(s) 114, 358
BstV2I GAAGAC 1 cut(s) 439
BstX2I RGATCY 1 cut(s) 551
BstYI RGATCY 1 cut(s) 551
BstZ17I GTATAC 1 cut(s) 55
BsuRI GGCC 2 cut(s) 14, 220
BtsCI GGATG 3 cut(s) 178, 259, 319
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 4 cut(s) 429, 508, 568, 614
Cac8I GCNNGC 1 cut(s) 436
CciI TCATGA 1 cut(s) 157
CfoI GCGC 1 cut(s) 381
Cfr13I GGNCC 2 cut(s) 218, 285
CviAII CATG 2 cut(s) 158, 384
CviJI RGCY 4 cut(s) 14, 220, 438, 491
CviKI_1 RGCY 4 cut(s) 14, 220, 438, 491
DdeI CTNAG 1 cut(s) 16
DpnI GATC 3 cut(s) 189, 393, 553
DpnII GATC 3 cut(s) 187, 391, 551
Eco130I CCWWGG 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 2 cut(s) 161, 387
FaiI YATR 5 cut(s) 55, 159, 282, 385, 559
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FatI CATG 2 cut(s) 157, 383
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 2 cut(s) 54, 587
Fnu4HI GCNGC 2 cut(s) 103, 347
FokI GGATG 3 cut(s) 185, 266, 326
Fsp4HI GCNGC 2 cut(s) 103, 347
FspBI CTAG 1 cut(s) 435
GlaI GCGC 1 cut(s) 380
GluI GCNGC 2 cut(s) 103, 347
GsuI CTGGAG 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 382
HaeIII GGCC 2 cut(s) 14, 220
HhaI GCGC 1 cut(s) 381
Hin1II CATG 2 cut(s) 161, 387
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 1 cut(s) 38
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 4 cut(s) 55, 146, 247, 588
Hpy188I TCNGA 3 cut(s) 19, 133, 205
Hpy188III TCNNGA 4 cut(s) 158, 495, 509, 597
Hpy8I GTNNAC 4 cut(s) 55, 146, 247, 588
HpyCH4III ACNGT 4 cut(s) 52, 503, 572, 585
HpyCH4V TGCA 4 cut(s) 24, 120, 209, 557
HpyF3I CTNAG 1 cut(s) 16
Hsp92II CATG 2 cut(s) 161, 387
HspAI GCGC 1 cut(s) 379
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 3 cut(s) 187, 391, 551
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 8 cut(s) 27, 201, 303, 456, 480, 494, 582, 620
Lsp1109I GCAGC 2 cut(s) 114, 358
LweI GCATC 4 cut(s) 196, 218, 276, 304
MaeI CTAG 1 cut(s) 435
MalI GATC 3 cut(s) 189, 393, 553
MboI GATC 3 cut(s) 187, 391, 551
MboII GAAGA 7 cut(s) 191, 194, 197, 204, 248, 348, 439
MflI RGATCY 1 cut(s) 551
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 4 cut(s) 70, 115, 277, 322
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 26
MnlI CCTC 6 cut(s) 25, 160, 163, 231, 306, 309
Mph1103I ATGCAT 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 467
MseI TTAA 1 cut(s) 543
MslI CAYNNNNRTG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 26
NdeII GATC 3 cut(s) 187, 391, 551
NheI GCTAGC 1 cut(s) 434
NlaIII CATG 2 cut(s) 161, 387
NlaIV GGNNCC 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 211
PagI TCATGA 1 cut(s) 157
PctI GAATGC 1 cut(s) 26
PdmI GAANNNNTTC 1 cut(s) 467
PkrI GCNGC 2 cut(s) 104, 348
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 285
PspN4I GGNNCC 1 cut(s) 286
PspPI GGNCC 2 cut(s) 218, 285
PspPPI RGGWCCY 1 cut(s) 285
PsuI RGATCY 1 cut(s) 551
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 2 cut(s) 103, 347
Sau3AI GATC 3 cut(s) 187, 391, 551
Sau96I GGNCC 2 cut(s) 218, 285
SchI GAGTC 1 cut(s) 47
SduI GDGCHC 1 cut(s) 66
SetI ASST 4 cut(s) 376, 440, 493, 526
SfaNI GCATC 4 cut(s) 196, 218, 276, 304
SinI GGWCC 1 cut(s) 285
SmiMI CAYNNNNRTG 1 cut(s) 231
Sse9I AATT 4 cut(s) 70, 115, 277, 322
SspMI CTAG 1 cut(s) 435
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 4 cut(s) 52, 503, 572, 585
TasI AATT 4 cut(s) 70, 115, 277, 322
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TscAI CASTG 4 cut(s) 436, 508, 575, 614
TseI GCWGC 2 cut(s) 102, 346
TspDTI ATGAA 7 cut(s) 17, 146, 192, 249, 255, 270, 460
TspRI CASTG 4 cut(s) 436, 508, 575, 614
VpaK11BI GGWCC 1 cut(s) 285
XapI RAATTY 1 cut(s) 322
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XmiI GTMKAC 2 cut(s) 54, 587
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 1 cut(s) 435
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.