MD15G1126200.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001247
Physical Location & Seq
Forward (+)
9172389 .. 9176715
4327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1126200.v1.1.491

Sequence Viewer

Length: 2178 bp
ATGCATTGCTCTTTATATCGTTTGTTTGGAATATTACTTATAATCGAACGGGAAAAGAAAAAGGCAAAGAAAAAGGAAAAGGATAATGAAAGAGAATTAAGAGGGGGCCCAGCAGCTTCACCGATTCTAGCCCAACTAGGACTTGAACTAGTTCTCATTTCCTCTCTCAGATTCCAGACCTCTCTCTCTCAGACTGAGTGGTGTGTTACGTATGTAAAGTTCACTCTAAATTCGTCGAAATATCAAACTCATCGGAGCTCATCACCAAGTTTCAGTCGAGAAATGGCTAAACAAGTTGTTGCTAACCAACCGTCAGCTTCATTTGAGTACGAGCTTTTAGATCGTGATTCTGATGGCCTGAGAACTATTGGAGCCTTTTCAGAACAGACAAGTCCTTGGATTGAACCTGTAAAATTGAAACTTAGACACAGAATTGGGAGGGGACCCTTTGGTGATGTTTGGTTAGCAACCCATCATCAGTCAACAGAAGATTATGATGAGTATCATGAGGTGGCTATCAAGATGTTCCATTCAATCAAAGAAGACAACATGAGGGCTGTGTTGGATAAACTGGCTGATATTTTTCACAAATGTCAAGGAGTTGGCGGTGTTTGTTGGCTATATGGAACTTCAATCATCAGTGGAAAGATATGCATCATCATGAGATTATATGAGGGATCCGTTGGTGACAAAATGGCACAACTCAGAGGAGGGAAGCTCTCACTTCCTGATGTTTTGAGGTATGGGATTGGTTTGGCTGCGGGGATTCTTGAACTACACTCCAAAGGCATCTTGGTTCTTAATCTTAAACCTTCTAATGTTCTTCTGAATGAAAAGGACCAAGCAATTCTTGGTGATTTTGGTATTCCTTATCTATTGCTTGGCGTACCATTGATAAGCTCAGATATGACTCGGCGGATTGGAACCCCAAACTACATGGCACCTGAACAATGGCAACCAGAAGTAAGAGGTCCAATATCTTTTGAGACCGACTCGTGGGGTTTTGGGTGCAGCATTGTGGAAATGTTGACCGGTGTCCGTCCTTGGGGCGGAAAATCTGTTGATGAAATATCTTATTTAGTTGTTGGAAAGCAAGAAAAACCAGACATACCAAGTGGCCTTCCTCCTGCAATAGAGAATATTCTTCTTGGTTGCTTTGAGTACGATTTGAGGAGTCGCCCTTTAATAACGGACATATTGACTTTATTCAAAAGCTTGCAGAGTACAATCTCCAGTGATGGAGACTGGACGGGTCTTGGGATAAGAACAATCAAGGAAAAATCGAGTAGCATTGGCTACACTGAGTGGTTCCTTTCAAAGGATCAACTGCAAGTGGGTGACACGGTGCGTTCCAGAAAGCCACCAAACTGCTGCAAGCCTGAAAACATGCATATTCCAGAAGGCACAGTAGTGGGTGTCGAACATGATGCAGATCAACATGGGTTTGATTTGGTGAGAGTCCATGGCATCCATGACCCACTGAGGGTTCATGTTTCGACTCTGGAGCGGGTCACTTTTGGCTTGGCAGCTGGTGATTGGGTACGCTTGAAGAAAGAAGACAAGAAACACTCACCAGTAGGTATTCTTCATTCCATCAATCGTGATGGTAATGTAGCTGTTGGATTTATAGGGTTGGAAACTCTCTGGAAGGGAAATTCTTCAGAGTTTCAGATGGCAGAGTCCTACTGTGTGGGTCAGTTTGCCAAGCTGAAAGCTAATGTATTGAGCCCTCGATTTGAATGGCCCCGTAAGAGGAAAGGGACATGGGCCACCGGGAAAATAACGTGGATCCTACCAAATGGGTGCCTTGTTGTCAAGTTCCCGGGAATGCTGACCTTTGGGGAAGAGAACTCAACCTTTGTAGCTGATCCTGCTGAAGTGGAATTGGTTACGTTTGATACATGTCCAGGAATAGTGAAGAAGTACCAACATCTTGAGGATTTTCATTGGGCAGTGAGGCCACTTCTGGTTGCATTGGGCATATTTACAGCCATGAAGCTAGGCATTTTTGTTGGAACAAAGGCGGGGGGGTCCAGGGTGAAGAAGCAACAGAGCGGTGCTACTCCGAATGAGATTCAGCATATCGATGGTCAGAATTCTGGAAACCCGGCGTGGATTCCTCCTAATATGAAAAATATCCTTTTCAGAGAAGGTGTTACCACCGGTCCTGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

726

Amino Acids

80.65

Weight (kDa)

8.52

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 139 - 401 4.6e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 142 - 400 2.3e-25 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
AccB1I GGYRCC 2 cut(s) 940, 1803
AccBSI CCGCTC 2 cut(s) 1507, 2055
AciI CCGC 7 cut(s) 606, 761, 916, 1050, 1507, 2024, 2055
AclWI GGATC 6 cut(s) 672, 685, 1329, 1783, 1796, 1862
AcsI RAATTY 3 cut(s) 229, 1654, 2095
AcuI CTGAAG 2 cut(s) 1644, 1896
AdeI CACNNNGTG 1 cut(s) 1305
AfaI GTAC 6 cut(s) 329, 888, 1163, 1225, 1542, 1925
AfiI CCNNNNNNNGG 6 cut(s) 996, 1045, 1049, 1318, 1483, 1752
AflIII ACRYGT 1 cut(s) 1901
AgeI ACCGGT 2 cut(s) 1031, 2162
AhlI ACTAGT 1 cut(s) 148
AjnI CCWGG 2 cut(s) 1906, 2033
AleI CACNNNNGTG 1 cut(s) 1409
Alw21I GWGCWC 1 cut(s) 260
Alw26I GTCTC 2 cut(s) 980, 1236
AlwI GGATC 6 cut(s) 672, 685, 1329, 1783, 1796, 1862
Ama87I CYCGRG 1 cut(s) 1822
AoxI GGCC 6 cut(s) 106, 355, 1117, 1742, 1767, 1958
ApaI GGGCCC 1 cut(s) 110
ApeKI GCWGC 5 cut(s) 113, 758, 1011, 1371, 1526
ApoI RAATTY 3 cut(s) 229, 1654, 2095
AsiGI ACCGGT 2 cut(s) 1031, 2162
Asp700I GAANNNNTTC 2 cut(s) 150, 1657
AspS9I GGNCC 9 cut(s) 106, 107, 443, 838, 971, 1743, 1767, 2031, 2165
AsuC2I CCSGG 4 cut(s) 1774, 1823, 1824, 2108
AvaI CYCGRG 1 cut(s) 1822
AvaII GGWCC 5 cut(s) 443, 838, 971, 2031, 2165
BaeGI GKGCMC 1 cut(s) 110
BamHI GGATCC 2 cut(s) 677, 1788
BanI GGYRCC 2 cut(s) 940, 1803
BanII GRGCYC 3 cut(s) 110, 260, 1730
BarI GAAGNNNNNNTAC 2 cut(s) 1569, 1601
BauI CACGAG 1 cut(s) 994
BbsI GAAGAC 2 cut(s) 549, 1563
Bbv12I GWGCWC 1 cut(s) 260
BbvI GCAGC 5 cut(s) 125, 745, 1023, 1358, 1538
BccI CCATC 7 cut(s) 347, 480, 1232, 1598, 1601, 1666, 2081
BcgI CGANNNNNNTGC 2 cut(s) 1409, 1443
BciT130I CCWGG 2 cut(s) 1908, 2035
BcnI CCSGG 4 cut(s) 1774, 1823, 1824, 2108
BcoDI GTCTC 2 cut(s) 980, 1236
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 4 cut(s) 128, 137, 149, 2000
BisI GCNGC 5 cut(s) 114, 759, 1012, 1372, 1527
BlsI GCNGC 5 cut(s) 115, 760, 1013, 1373, 1528
Bme1390I CCNGG 6 cut(s) 1774, 1823, 1824, 1908, 2035, 2108
Bme18I GGWCC 5 cut(s) 443, 838, 971, 2031, 2165
BmeT110I CYCGRG 1 cut(s) 1822
BmgT120I GGNCC 9 cut(s) 106, 107, 443, 838, 971, 1743, 1767, 2031, 2165
BmrFI CCNGG 6 cut(s) 1774, 1823, 1824, 1908, 2035, 2108
BmsI GCATC 4 cut(s) 663, 798, 1417, 1476
BoxI GACNNNNGTC 1 cut(s) 1034
BpiI GAAGAC 2 cut(s) 549, 1563
BplI GAGNNNNNCTC 4 cut(s) 702, 734, 977, 1009
BpmI CTGGAG 2 cut(s) 1216, 1523
BpuEI CTTGAG 1 cut(s) 1955
BpuMI CCSGG 4 cut(s) 1774, 1823, 1824, 2108
Bsa29I ATCGAT 1 cut(s) 2085
BsaAI YACGTR 1 cut(s) 210
BsaBI GATNNNNATC 3 cut(s) 501, 653, 1431
BsaI GGTCTC 1 cut(s) 980
BsaJI CCNNGG 5 cut(s) 395, 1043, 1462, 1822, 2034
BsaWI WCCGGW 2 cut(s) 1031, 2162
Bsc4I CCNNNNNNNGG 6 cut(s) 996, 1045, 1049, 1318, 1483, 1752
Bse118I RCCGGY 2 cut(s) 1031, 2162
Bse1I ACTGG 4 cut(s) 576, 1233, 1250, 1574
Bse3DI GCAATG 1 cut(s) 4
Bse8I GATNNNNATC 3 cut(s) 501, 653, 1431
BseBI CCWGG 2 cut(s) 1908, 2035
BseCI ATCGAT 1 cut(s) 2085
BseDI CCNNGG 5 cut(s) 395, 1043, 1462, 1822, 2034
BseGI GGATG 1 cut(s) 1467
BseJI GATNNNNATC 3 cut(s) 501, 653, 1431
BseLI CCNNNNNNNGG 6 cut(s) 996, 1045, 1049, 1318, 1483, 1752
BseMI GCAATG 1 cut(s) 4
BseMII CTCAG 8 cut(s) 181, 186, 203, 350, 718, 915, 1293, 1472
BseNI ACTGG 4 cut(s) 576, 1233, 1250, 1574
BseRI GAGGAG 2 cut(s) 723, 1186
BseSI GKGCMC 1 cut(s) 110
BseXI GCAGC 5 cut(s) 125, 745, 1023, 1358, 1538
BseYI CCCAGC 1 cut(s) 109
BsgI GTGCAG 1 cut(s) 1030
BshFI GGCC 6 cut(s) 108, 357, 1119, 1744, 1769, 1960
BshNI GGYRCC 2 cut(s) 940, 1803
BshTI ACCGGT 2 cut(s) 1031, 2162
BshVI ATCGAT 1 cut(s) 2085
BsiHKAI GWGCWC 1 cut(s) 260
BsiHKCI CYCGRG 1 cut(s) 1822
BsiSI CCGG 5 cut(s) 1032, 1773, 1823, 2108, 2163
BslFI GGGAC 2 cut(s) 456, 1774
BslI CCNNNNNNNGG 6 cut(s) 996, 1045, 1049, 1318, 1483, 1752
BsmAI GTCTC 2 cut(s) 980, 1236
BsmFI GGGAC 2 cut(s) 456, 1774
BsmI GAATGC 1 cut(s) 1833
BsnI GGCC 6 cut(s) 108, 357, 1119, 1744, 1769, 1960
Bso31I GGTCTC 1 cut(s) 980
BsoBI CYCGRG 1 cut(s) 1822
Bsp120I GGGCCC 1 cut(s) 106
Bsp1286I GDGCHC 3 cut(s) 110, 260, 1730
Bsp143I GATC 6 cut(s) 340, 677, 1321, 1432, 1788, 1867
Bsp19I CCATGG 1 cut(s) 1462
BspACI CCGC 7 cut(s) 606, 761, 916, 1050, 1507, 2024, 2055
BspANI GGCC 6 cut(s) 108, 357, 1119, 1744, 1769, 1960
BspCNI CTCAG 8 cut(s) 180, 187, 202, 351, 717, 914, 1294, 1473
BspDI ATCGAT 1 cut(s) 2085
BspHI TCATGA 2 cut(s) 505, 660
BspPI GGATC 6 cut(s) 672, 685, 1329, 1783, 1796, 1862
BspT107I GGYRCC 2 cut(s) 940, 1803
BspTNI GGTCTC 1 cut(s) 980
BsrBI CCGCTC 2 cut(s) 1507, 2055
BsrDI GCAATG 1 cut(s) 4
BsrFI RCCGGY 2 cut(s) 1031, 2162
BsrI ACTGG 4 cut(s) 576, 1233, 1250, 1574
BssAI RCCGGY 2 cut(s) 1031, 2162
BssECI CCNNGG 5 cut(s) 395, 1043, 1462, 1822, 2034
BssMI GATC 6 cut(s) 340, 677, 1321, 1432, 1788, 1867
BssSI CACGAG 1 cut(s) 994
BssT1I CCWWGG 3 cut(s) 395, 1043, 1462
Bst2BI CACGAG 1 cut(s) 994
Bst2UI CCWGG 2 cut(s) 1908, 2035
Bst4CI ACNGT 4 cut(s) 312, 1345, 1408, 1688
Bst6I CTCTTC 1 cut(s) 1839
BstBAI YACGTR 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 1217, 1376
BstDEI CTNAG 9 cut(s) 167, 189, 195, 359, 422, 704, 901, 1302, 1481
BstDSI CCRYGG 1 cut(s) 1462
BstENI CCTNNNNNAGG 1 cut(s) 1316
BstF5I GGATG 1 cut(s) 1467
BstKTI GATC 6 cut(s) 343, 680, 1324, 1435, 1791, 1870
BstMAI GTCTC 2 cut(s) 980, 1236
BstMBI GATC 6 cut(s) 340, 677, 1321, 1432, 1788, 1867
BstMWI GCNNNNNNNGC 1 cut(s) 1871
BstNI CCWGG 2 cut(s) 1908, 2035
BstNSI RCATGY 2 cut(s) 1390, 1905
BstPAI GACNNNNGTC 1 cut(s) 1034
BstSCI CCNGG 6 cut(s) 1772, 1821, 1822, 1906, 2033, 2106
BstSLI GKGCMC 1 cut(s) 110
BstSNI TACGTA 1 cut(s) 210
BstV1I GCAGC 5 cut(s) 125, 745, 1023, 1358, 1538
BstV2I GAAGAC 2 cut(s) 549, 1563
BstX2I RGATCY 2 cut(s) 677, 1788
BstYI RGATCY 2 cut(s) 677, 1788
Bsu15I ATCGAT 1 cut(s) 2085
BsuRI GGCC 6 cut(s) 108, 357, 1119, 1744, 1769, 1960
BsuTUI ATCGAT 1 cut(s) 2085
BtgI CCRYGG 1 cut(s) 1462
BtsCI GGATG 1 cut(s) 1467
BtsI GCAGTG 1 cut(s) 1959
BtsIMutI CAGTG 5 cut(s) 646, 1240, 1299, 1478, 1959
Cac8I GCNNGC 2 cut(s) 1217, 1376
CciI TCATGA 2 cut(s) 505, 660
Cfr10I RCCGGY 2 cut(s) 1031, 2162
Cfr13I GGNCC 9 cut(s) 106, 107, 443, 838, 971, 1743, 1767, 2031, 2165
Cfr9I CCCGGG 1 cut(s) 1822
ClaI ATCGAT 1 cut(s) 2085
Csp6I GTAC 6 cut(s) 328, 887, 1162, 1224, 1541, 1924
CspAI ACCGGT 2 cut(s) 1031, 2162
CviQI GTAC 6 cut(s) 328, 887, 1162, 1224, 1541, 1924
DdeI CTNAG 9 cut(s) 167, 189, 195, 359, 422, 704, 901, 1302, 1481
DpnI GATC 6 cut(s) 342, 679, 1323, 1434, 1790, 1869
DpnII GATC 6 cut(s) 340, 677, 1321, 1432, 1788, 1867
DraIII CACNNNGTG 1 cut(s) 1305
Eam1104I CTCTTC 1 cut(s) 1839
EarI CTCTTC 1 cut(s) 1839
EciI GGCGGA 2 cut(s) 931, 1065
Ecl136II GAGCTC 1 cut(s) 258
Eco105I TACGTA 1 cut(s) 210
Eco130I CCWWGG 3 cut(s) 395, 1043, 1462
Eco24I GRGCYC 3 cut(s) 110, 260, 1730
Eco31I GGTCTC 1 cut(s) 980
Eco47I GGWCC 5 cut(s) 443, 838, 971, 2031, 2165
Eco53kI GAGCTC 1 cut(s) 258
Eco57I CTGAAG 2 cut(s) 1644, 1896
Eco88I CYCGRG 1 cut(s) 1822
EcoICRI GAGCTC 1 cut(s) 258
EcoNI CCTNNNNNAGG 1 cut(s) 1316
EcoO109I RGGNCCY 2 cut(s) 106, 443
EcoRI GAATTC 1 cut(s) 2095
EcoRII CCWGG 2 cut(s) 1906, 2033
EcoT14I CCWWGG 3 cut(s) 395, 1043, 1462
EcoT22I ATGCAT 3 cut(s) 6, 656, 1392
EcoT38I GRGCYC 3 cut(s) 110, 260, 1730
ErhI CCWWGG 3 cut(s) 395, 1043, 1462
FalI AAGNNNNNCTT 2 cut(s) 834, 866
FaqI GGGAC 2 cut(s) 456, 1774
FauI CCCGC 3 cut(s) 754, 1500, 2017
Fnu4HI GCNGC 5 cut(s) 114, 759, 1012, 1372, 1527
FokI GGATG 1 cut(s) 1454
FriOI GRGCYC 3 cut(s) 110, 260, 1730
Fsp4HI GCNGC 5 cut(s) 114, 759, 1012, 1372, 1527
FspBI CTAG 4 cut(s) 128, 137, 149, 2000
GluI GCNGC 5 cut(s) 114, 759, 1012, 1372, 1527
GsaI CCCAGC 1 cut(s) 113
GsuI CTGGAG 2 cut(s) 1216, 1523
HaeIII GGCC 6 cut(s) 108, 357, 1119, 1744, 1769, 1960
HapII CCGG 5 cut(s) 1032, 1773, 1823, 2108, 2163
HincII GTYRAC 2 cut(s) 483, 1029
HindII GTYRAC 2 cut(s) 483, 1029
HindIII AAGCTT 1 cut(s) 1213
HpaII CCGG 5 cut(s) 1032, 1773, 1823, 2108, 2163
Hpy166II GTNNAC 3 cut(s) 222, 483, 1029
Hpy8I GTNNAC 3 cut(s) 222, 483, 1029
Hpy99I CGWCG 1 cut(s) 238
HpyAV CCTTC 5 cut(s) 822, 1130, 1394, 1642, 2144
HpyCH4III ACNGT 4 cut(s) 312, 1345, 1408, 1688
HpyCH4IV ACGT 3 cut(s) 209, 1784, 1892
HpyF10VI GCNNNNNNNGC 1 cut(s) 1871
HpyF3I CTNAG 9 cut(s) 167, 189, 195, 359, 422, 704, 901, 1302, 1481
HpySE526I ACGT 3 cut(s) 209, 1784, 1892
KflI GGGWCCC 1 cut(s) 443
Kzo9I GATC 6 cut(s) 340, 677, 1321, 1432, 1788, 1867
LmnI GCTCC 3 cut(s) 255, 371, 1504
Lsp1109I GCAGC 5 cut(s) 125, 745, 1023, 1358, 1538
LweI GCATC 4 cut(s) 663, 798, 1417, 1476
MaeI CTAG 4 cut(s) 128, 137, 149, 2000
MaeII ACGT 3 cut(s) 209, 1784, 1892
MaeIII GTNAC 6 cut(s) 205, 686, 1337, 1510, 1888, 2155
MalI GATC 6 cut(s) 342, 679, 1323, 1434, 1790, 1869
MbiI CCGCTC 2 cut(s) 1507, 2055
MboI GATC 6 cut(s) 340, 677, 1321, 1432, 1788, 1867
MflI RGATCY 2 cut(s) 677, 1788
MhlI GDGCHC 3 cut(s) 110, 260, 1730
MluCI AATT 8 cut(s) 95, 229, 413, 432, 846, 1654, 1883, 2095
MlyI GAGTC 6 cut(s) 904, 986, 1183, 1467, 1492, 1688
MmeI TCCRAC 5 cut(s) 543, 1066, 1600, 1614, 1993
Mph1103I ATGCAT 3 cut(s) 6, 656, 1392
MroXI GAANNNNTTC 2 cut(s) 150, 1657
MseI TTAA 4 cut(s) 98, 801, 807, 1184
MslI CAYNNNNRTG 3 cut(s) 659, 1409, 2085
MspA1I CMGCKG 1 cut(s) 1529
MspI CCGG 5 cut(s) 1032, 1773, 1823, 2108, 2163
MspR9I CCNGG 6 cut(s) 1774, 1823, 1824, 1908, 2035, 2108
Mva1269I GAATGC 1 cut(s) 1833
MvaI CCWGG 2 cut(s) 1908, 2035
MwoI GCNNNNNNNGC 1 cut(s) 1871
NciI CCSGG 4 cut(s) 1774, 1823, 1824, 2108
NcoI CCATGG 1 cut(s) 1462
NdeII GATC 6 cut(s) 340, 677, 1321, 1432, 1788, 1867
NmeAIII GCCGAG 1 cut(s) 892
NmuCI GTSAC 3 cut(s) 686, 1337, 1510
NsiI ATGCAT 3 cut(s) 6, 656, 1392
NspI RCATGY 2 cut(s) 1390, 1905
OliI CACNNNNGTG 1 cut(s) 1409
PagI TCATGA 2 cut(s) 505, 660
PciI ACATGT 1 cut(s) 1901
PctI GAATGC 1 cut(s) 1833
PdmI GAANNNNTTC 2 cut(s) 150, 1657
PfeI GAWTC 6 cut(s) 124, 171, 347, 766, 2074, 2116
PfoI TCCNGGA 1 cut(s) 1906
PinAI ACCGGT 2 cut(s) 1031, 2162
PkrI GCNGC 5 cut(s) 115, 760, 1013, 1373, 1528
PleI GAGTC 6 cut(s) 904, 986, 1182, 1466, 1492, 1687
PpsI GAGTC 6 cut(s) 904, 986, 1182, 1466, 1492, 1687
Ppu21I YACGTR 1 cut(s) 210
PpuMI RGGWCCY 1 cut(s) 443
PscI ACATGT 1 cut(s) 1901
PshAI GACNNNNGTC 1 cut(s) 1034
PsiI TTATAA 1 cut(s) 41
Psp124BI GAGCTC 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 443
Psp6I CCWGG 2 cut(s) 1906, 2033
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 2 cut(s) 1906, 2033
PspOMI GGGCCC 1 cut(s) 106
PspPI GGNCC 9 cut(s) 106, 107, 443, 838, 971, 1743, 1767, 2031, 2165
PspPPI RGGWCCY 1 cut(s) 443
PsrI GAACNNNNNNTAC 2 cut(s) 203, 235
PsuI RGATCY 2 cut(s) 677, 1788
PvuII CAGCTG 1 cut(s) 1529
RsaI GTAC 6 cut(s) 329, 888, 1163, 1225, 1542, 1925
RsaNI GTAC 6 cut(s) 328, 887, 1162, 1224, 1541, 1924
RseI CAYNNNNRTG 3 cut(s) 659, 1409, 2085
SacI GAGCTC 1 cut(s) 260
SaqAI TTAA 4 cut(s) 98, 801, 807, 1184
SatI GCNGC 5 cut(s) 114, 759, 1012, 1372, 1527
Sau3AI GATC 6 cut(s) 340, 677, 1321, 1432, 1788, 1867
Sau96I GGNCC 9 cut(s) 106, 107, 443, 838, 971, 1743, 1767, 2031, 2165
SchI GAGTC 6 cut(s) 904, 986, 1183, 1467, 1492, 1688
ScrFI CCNGG 6 cut(s) 1774, 1823, 1824, 1908, 2035, 2108
SduI GDGCHC 3 cut(s) 110, 260, 1730
SfaNI GCATC 4 cut(s) 663, 798, 1417, 1476
SinI GGWCC 5 cut(s) 443, 838, 971, 2031, 2165
SmaI CCCGGG 1 cut(s) 1824
SmiMI CAYNNNNRTG 3 cut(s) 659, 1409, 2085
SmlI CTYRAG 1 cut(s) 1934
SmoI CTYRAG 1 cut(s) 1934
SnaBI TACGTA 1 cut(s) 210
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 8 cut(s) 95, 229, 413, 432, 846, 1654, 1883, 2095
SsiI CCGC 7 cut(s) 606, 761, 916, 1050, 1507, 2024, 2055
SspI AATATT 2 cut(s) 33, 1141
SspMI CTAG 4 cut(s) 128, 137, 149, 2000
SstI GAGCTC 1 cut(s) 260
StyD4I CCNGG 6 cut(s) 1772, 1821, 1822, 1906, 2033, 2106
StyI CCWWGG 3 cut(s) 395, 1043, 1462
TaaI ACNGT 4 cut(s) 312, 1345, 1408, 1688
TaiI ACGT 3 cut(s) 212, 1787, 1895
TaqI TCGA 8 cut(s) 45, 236, 277, 1283, 1419, 1496, 1732, 2085
TaqII GACCGA 1 cut(s) 1004
TasI AATT 8 cut(s) 95, 229, 413, 432, 846, 1654, 1883, 2095
TatI WGTACW 1 cut(s) 1223
TfiI GAWTC 6 cut(s) 124, 171, 347, 766, 2074, 2116
Tru1I TTAA 4 cut(s) 98, 801, 807, 1184
Tru9I TTAA 4 cut(s) 98, 801, 807, 1184
TscAI CASTG 5 cut(s) 646, 1240, 1306, 1485, 1959
TseFI GTSAC 3 cut(s) 686, 1337, 1510
TseI GCWGC 5 cut(s) 113, 758, 1011, 1371, 1526
Tsp45I GTSAC 3 cut(s) 686, 1337, 1510
TspDTI ATGAA 9 cut(s) 102, 309, 846, 1080, 1478, 1577, 1934, 2009, 2144
TspGWI ACGGA 3 cut(s) 670, 1028, 1205
TspMI CCCGGG 1 cut(s) 1822
TspRI CASTG 5 cut(s) 646, 1240, 1306, 1485, 1959
VpaK11BI GGWCC 5 cut(s) 443, 838, 971, 2031, 2165
XagI CCTNNNNNAGG 1 cut(s) 1316
XapI RAATTY 3 cut(s) 229, 1654, 2095
XceI RCATGY 2 cut(s) 1390, 1905
XcmI CCANNNNNNNNNTGG 2 cut(s) 790, 848
XmaI CCCGGG 1 cut(s) 1822
XmnI GAANNNNTTC 2 cut(s) 150, 1657
XspI CTAG 4 cut(s) 128, 137, 149, 2000
Zsp2I ATGCAT 3 cut(s) 6, 656, 1392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.