pycom17g21810

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001247
Physical Location & Seq
Forward (+)
20239166 .. 20241882
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g21810.1

Sequence Viewer

Length: 1887 bp
ATGGGAAAAGCAAAGCCGGCAGTCTCTTTTGAGTATGAGCTCTTTGAGGGAGATCCGGACCACCTAAGAACTGTAGTGGCTACACTCACGCCAACGCTTACACCTACTAATCCATGGATCAGCCCTGATTCGTTGAACTTCAAATACCGAATTGGGCGAGGCCCCTTTGGTGATGTTTGGTTAGCAACTCATCATCAGTCTGCAGATGATTATGATGAGTATCATGAGGTAGCTGTTAAGATGCTGCACCCTTTGAAGGAGGAAGACACTGAGAAGTTTGTGCATAAGGTTGAAAGGTTATTTTTCAAGTTCCGCAGAGTCTATGGCCTTTGTTGGCTTCACGGCATCTCAGTTATTGATGGGAAGGTCTGCATTGCTATGAAATTTTATGAGGGATCAATTGGTGATCGAGTTGCTCGGATGAAGGGCGGAAAGCTTCAACTTTCTGATGTTTTAAGGTATGGGATTGAGTTGGCGAAAGGAATTTTAGACTTGCACTCACTTGGGATTCTGGTGCTAAACCTGAAACCTTCCAACTTCCTTTTAGATGAACATGACCAGGCGGTCCTTGGAGATTTTGGGATTCCGTATCTACTTCTTGGGATTTCATTGTCTAATTCAGATATGGCTCTCAGACTTGGAACCCCAAACTACATGGCTCCCGAACAGTGGGAACCAGAAGTGAGGGGCCCTGTATCCTTAGAAACGGATGCCTGGGGTTTTGGATGTTGCTTTGTGGAGATGTTGACTGGCGTTCAACCATGGTTTGGGAGGTCAATTGAAGAGATATGTCATTCAGTTGTGATAAAGCAAGAAAAACCACCTGTTCCAAGCGGCTTGCCTCCTGCAGTTGAGAATGTTATAAGTGGTTGCTTTGAGTATGACTTCCGCAATCGGCCTTCAATGGATGATATAATACATGCTTTTGAAAGCTCACAGAATGATGTTCAAAGTGAAGGCGAGTGGCTTGGTTTAGGGAGCAGTACAGTTGCTGGAAAACATAATAGCGGTGGTTATACTACATGGTATCTCTCAAAAGATCCTCTTCAGGTTGGAGATATAGTTCGCTCAAGAAAGCCCCTAAATGCACGCAAGCCTCAAGCTATGGATATCCCTGAAGGAACTGTCGCTGGTCTGGAGACTGATTCTGATAGAGAAGGTTTTGCCCTGGTGAAGATCCCCGGAGTGCGCAACCCTGTAAGAGTGCTGGTGTCAACTGTGGAGAGAGTCACATCTGGCTTAGCAGTGGGTGATTGGGTGCGCTTGAAGGGTGAAGGCAGGAGGCACTCTGCTGTTGGAGTTCTTCATTCGATACAGCGTGATGGTAGTGTTGCAGTTGGATTTATTGGGTTAGAGACTCTATGGAGAGGGAAGTCATCCGACCTTCAAATGACAGATTCATATTTTGTCGGAGACTTTGTGAGGCTGAAGGCTAGTGTGTTCTCTCCTAGATTTGAGTGGCCTCGTAAGAATGGAGGAGCATGGGCGTCTGGAAGGATCTCACAAGTCCTTTCGAATGGCTGTCTGGTTGTGAGGTTTCCCGGAAGATTTGTACTCGGAGATGAATCTAATAGCTTCTTGGCGGATCCAGCTGAAGTAGAACTAGTGTCCTTTGATACATGTCCTGGAGTGGTCGGAAAATATCAACACATCGAGGATTTTCACTGGGCAGTGAGGCCTCTTGCAATTGCGTTTGGCCTATTTACAGCCATGAAGGTGAGCTTATTTGTTGGACAGAATGTAGGTGCAAAGCTGAAGGGTCGAAGATCAACACGGAAGGATGGGAATAACCAAGATGGTCAGAGTGGTGGCGGTGCAGGTTGGATTCCTCCAACAGTTGCGAATATTCTTTTTAAAGAAAGCGTTCCTACTGCAGGCGCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1 Pkinase Pkinase_Tyr

Protein Analysis

629

Amino Acids

69.19

Weight (kDa)

6.27

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 46 - 308 2.9e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 48 - 307 1.1e-26 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 863
AasI GACNNNNNNGTC 1 cut(s) 563
Acc16I TGCGCA 1 cut(s) 1190
Acc36I ACCTGC 1 cut(s) 1808
AccB7I CCANNNNNTGG 1 cut(s) 767
AccIII TCCGGA 1 cut(s) 55
AciI CCGC 8 cut(s) 313, 429, 563, 834, 889, 1008, 1583, 1812
AclWI GGATC 8 cut(s) 47, 125, 403, 1034, 1171, 1505, 1580, 1593
AcsI RAATTY 2 cut(s) 383, 483
AcuI CTGAAG 5 cut(s) 1031, 1137, 1448, 1614, 1775
AcyI GRCGYC 1 cut(s) 1487
AfaI GTAC 2 cut(s) 985, 1554
AfiI CCNNNNNNNGG 4 cut(s) 256, 669, 767, 1874
AflIII ACRYGT 1 cut(s) 1619
AhlI ACTAGT 1 cut(s) 1603
AjnI CCWGG 4 cut(s) 558, 713, 1167, 1624
AloI GAACNNNNNNTCC 2 cut(s) 1047, 1079
AluBI AGCT 9 cut(s) 40, 233, 436, 933, 1103, 1575, 1592, 1722, 1753
AluI AGCT 9 cut(s) 40, 233, 436, 933, 1103, 1575, 1592, 1722, 1753
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 4 cut(s) 28, 1133, 1349, 1407
AlwI GGATC 8 cut(s) 47, 125, 403, 1034, 1171, 1505, 1580, 1593
AlwNI CAGNNNCTG 1 cut(s) 992
Aor13HI TCCGGA 1 cut(s) 55
AoxI GGCC 7 cut(s) 160, 325, 688, 896, 1460, 1676, 1696
ApaI GGGCCC 1 cut(s) 692
ApeKI GCWGC 1 cut(s) 244
ApoI RAATTY 2 cut(s) 383, 483
Asp700I GAANNNNTTC 1 cut(s) 1863
AspLEI GCGC 3 cut(s) 1191, 1263, 1880
AspS9I GGNCC 5 cut(s) 58, 161, 565, 688, 689
AsuC2I CCSGG 2 cut(s) 1182, 1542
AsuHPI GGTGA 6 cut(s) 182, 416, 1183, 1262, 1283, 1729
AsuII TTCGAA 1 cut(s) 1514
AvaII GGWCC 2 cut(s) 58, 565
BaeGI GKGCMC 1 cut(s) 692
BamHI GGATCC 1 cut(s) 1585
BanII GRGCYC 2 cut(s) 42, 692
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 231
BccI CCATC 4 cut(s) 353, 1316, 1775, 1790
BceAI ACGGC 1 cut(s) 358
BciT130I CCWGG 4 cut(s) 560, 715, 1169, 1626
BciVI GTATCC 1 cut(s) 706
BcnI CCSGG 2 cut(s) 1182, 1542
BcoDI GTCTC 4 cut(s) 28, 1133, 1349, 1407
BcuI ACTAGT 1 cut(s) 1603
BfaI CTAG 4 cut(s) 1434, 1449, 1604, 1881
BfmI CTRYAG 4 cut(s) 72, 201, 846, 1872
BfoI RGCGCY 1 cut(s) 1881
BfuAI ACCTGC 1 cut(s) 1808
BfuI GTATCC 1 cut(s) 706
BisI GCNGC 2 cut(s) 245, 835
BlpI GCTNAGC 1 cut(s) 1240
BlsI GCNGC 2 cut(s) 246, 836
Bme1390I CCNGG 6 cut(s) 560, 715, 1169, 1182, 1542, 1626
Bme18I GGWCC 2 cut(s) 58, 565
BmgT120I GGNCC 5 cut(s) 58, 161, 565, 688, 689
BmiI GGNNCC 7 cut(s) 163, 643, 660, 675, 689, 690, 1587
BmrFI CCNGG 6 cut(s) 560, 715, 1169, 1182, 1542, 1626
BmrI ACTGGG 1 cut(s) 1675
BmsI GCATC 3 cut(s) 231, 354, 700
BmuI ACTGGG 1 cut(s) 1675
BpiI GAAGAC 1 cut(s) 270
BpmI CTGGAG 2 cut(s) 1157, 1647
Bpu1102I GCTNAGC 1 cut(s) 1240
Bpu14I TTCGAA 1 cut(s) 1514
BpuEI CTTGAG 2 cut(s) 1054, 1083
BpuMI CCSGG 2 cut(s) 1182, 1542
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 1487
BsaJI CCNNGG 6 cut(s) 113, 568, 714, 761, 1167, 1180
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 4 cut(s) 1047, 1077, 1404, 1434
Bsc4I CCNNNNNNNGG 4 cut(s) 256, 669, 767, 1874
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 754, 1670
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 219
BseAI TCCGGA 1 cut(s) 55
BseBI CCWGG 4 cut(s) 560, 715, 1169, 1626
BseDI CCNNGG 6 cut(s) 113, 568, 714, 761, 1167, 1180
BseGI GGATG 6 cut(s) 426, 715, 731, 913, 1376, 1786
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 4 cut(s) 256, 669, 767, 1874
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 3 cut(s) 261, 363, 646
BseNI ACTGG 2 cut(s) 754, 1670
BseRI GAGGAG 1 cut(s) 1491
BseSI GKGCMC 1 cut(s) 692
BseXI GCAGC 1 cut(s) 231
BsgI GTGCAG 2 cut(s) 230, 1836
BshFI GGCC 7 cut(s) 162, 327, 690, 898, 1462, 1678, 1698
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 4 cut(s) 17, 56, 1182, 1542
BslI CCNNNNNNNGG 4 cut(s) 256, 669, 767, 1874
BsmAI GTCTC 4 cut(s) 28, 1133, 1349, 1407
BsnI GGCC 7 cut(s) 162, 327, 690, 898, 1462, 1678, 1698
Bsp119I TTCGAA 1 cut(s) 1514
Bsp120I GGGCCC 1 cut(s) 688
Bsp1286I GDGCHC 2 cut(s) 42, 692
Bsp13I TCCGGA 1 cut(s) 55
Bsp143I GATC 9 cut(s) 52, 117, 395, 406, 1039, 1176, 1497, 1585, 1766
Bsp1720I GCTNAGC 1 cut(s) 1240
Bsp19I CCATGG 2 cut(s) 113, 761
BspACI CCGC 8 cut(s) 313, 429, 563, 834, 889, 1008, 1583, 1812
BspANI GGCC 7 cut(s) 162, 327, 690, 898, 1462, 1678, 1698
BspCNI CTCAG 3 cut(s) 262, 362, 645
BspEI TCCGGA 1 cut(s) 55
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 7 cut(s) 163, 643, 660, 675, 689, 690, 1587
BspMAI CTGCAG 3 cut(s) 205, 850, 1876
BspMI ACCTGC 1 cut(s) 1808
BspPI GGATC 8 cut(s) 47, 125, 403, 1034, 1171, 1505, 1580, 1593
BspT104I TTCGAA 1 cut(s) 1514
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 2 cut(s) 754, 1670
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 6 cut(s) 113, 568, 714, 761, 1167, 1180
BssMI GATC 9 cut(s) 52, 117, 395, 406, 1039, 1176, 1497, 1585, 1766
BssNI GRCGYC 1 cut(s) 1487
BssT1I CCWWGG 3 cut(s) 113, 568, 761
Bst2UI CCWGG 4 cut(s) 560, 715, 1169, 1626
Bst4CI ACNGT 6 cut(s) 73, 669, 988, 1126, 1219, 1837
Bst6I CTCTTC 2 cut(s) 777, 1050
BstACI GRCGYC 1 cut(s) 1487
BstBI TTCGAA 1 cut(s) 1514
BstC8I GCNNGC 5 cut(s) 18, 839, 1090, 1094, 1876
BstDEI CTNAG 6 cut(s) 65, 270, 349, 632, 700, 1240
BstDSI CCRYGG 2 cut(s) 113, 761
BstENI CCTNNNNNAGG 1 cut(s) 1872
BstF5I GGATG 6 cut(s) 426, 715, 731, 913, 1376, 1786
BstH2I RGCGCY 1 cut(s) 1881
BstHHI GCGC 3 cut(s) 1191, 1263, 1880
BstKTI GATC 9 cut(s) 55, 120, 398, 409, 1042, 1179, 1500, 1588, 1769
BstMAI GTCTC 4 cut(s) 28, 1133, 1349, 1407
BstMBI GATC 9 cut(s) 52, 117, 395, 406, 1039, 1176, 1497, 1585, 1766
BstMWI GCNNNNNNNGC 2 cut(s) 17, 1589
BstNI CCWGG 4 cut(s) 560, 715, 1169, 1626
BstNSI RCATGY 2 cut(s) 923, 1623
BstSCI CCNGG 6 cut(s) 558, 713, 1167, 1180, 1540, 1624
BstSFI CTRYAG 4 cut(s) 72, 201, 846, 1872
BstSLI GKGCMC 1 cut(s) 692
BstV1I GCAGC 1 cut(s) 231
BstV2I GAAGAC 1 cut(s) 270
BstX2I RGATCY 5 cut(s) 52, 1039, 1176, 1497, 1585
BstYI RGATCY 5 cut(s) 52, 1039, 1176, 1497, 1585
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 7 cut(s) 162, 327, 690, 898, 1462, 1678, 1698
BtgI CCRYGG 2 cut(s) 113, 761
BtsCI GGATG 6 cut(s) 426, 715, 731, 913, 1376, 1786
BtsI GCAGTG 2 cut(s) 1251, 1677
BtsIMutI CAGTG 5 cut(s) 267, 674, 1251, 1663, 1677
BveI ACCTGC 1 cut(s) 1808
Cac8I GCNNGC 5 cut(s) 18, 839, 1090, 1094, 1876
CaiI CAGNNNCTG 1 cut(s) 992
CciI TCATGA 1 cut(s) 223
CfoI GCGC 3 cut(s) 1191, 1263, 1880
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 5 cut(s) 58, 161, 565, 688, 689
CseI GACGC 1 cut(s) 1476
Csp6I GTAC 2 cut(s) 984, 1553
CviQI GTAC 2 cut(s) 984, 1553
DdeI CTNAG 6 cut(s) 65, 270, 349, 632, 700, 1240
DpnI GATC 9 cut(s) 54, 119, 397, 408, 1041, 1178, 1499, 1587, 1768
DpnII GATC 9 cut(s) 52, 117, 395, 406, 1039, 1176, 1497, 1585, 1766
DraI TTTAAA 1 cut(s) 1855
DrdI GACNNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 563
Eam1104I CTCTTC 2 cut(s) 777, 1050
EarI CTCTTC 2 cut(s) 777, 1050
EciI GGCGGA 2 cut(s) 444, 1598
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 3 cut(s) 113, 568, 761
Eco147I AGGCCT 1 cut(s) 1678
Eco24I GRGCYC 2 cut(s) 42, 692
Eco32I GATATC 1 cut(s) 1111
Eco47I GGWCC 2 cut(s) 58, 565
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 5 cut(s) 1031, 1137, 1448, 1614, 1775
EcoICRI GAGCTC 1 cut(s) 40
EcoNI CCTNNNNNAGG 1 cut(s) 1872
EcoO109I RGGNCCY 3 cut(s) 161, 688, 689
EcoRII CCWGG 4 cut(s) 558, 713, 1167, 1624
EcoRV GATATC 1 cut(s) 1111
EcoT14I CCWWGG 3 cut(s) 113, 568, 761
EcoT38I GRGCYC 2 cut(s) 42, 692
ErhI CCWWGG 3 cut(s) 113, 568, 761
FalI AAGNNNNNCTT 4 cut(s) 1029, 1061, 1706, 1738
Fnu4HI GCNGC 2 cut(s) 245, 835
FokI GGATG 6 cut(s) 433, 722, 738, 920, 1363, 1793
FriOI GRGCYC 2 cut(s) 42, 692
Fsp4HI GCNGC 2 cut(s) 245, 835
FspBI CTAG 4 cut(s) 1434, 1449, 1604, 1881
FspI TGCGCA 1 cut(s) 1190
GlaI GCGC 3 cut(s) 1190, 1262, 1879
GluI GCNGC 2 cut(s) 245, 835
GsuI CTGGAG 2 cut(s) 1157, 1647
HaeII RGCGCY 1 cut(s) 1881
HaeIII GGCC 7 cut(s) 162, 327, 690, 898, 1462, 1678, 1698
HapII CCGG 4 cut(s) 17, 56, 1182, 1542
HgaI GACGC 1 cut(s) 1476
HhaI GCGC 3 cut(s) 1191, 1263, 1880
Hin1I GRCGYC 1 cut(s) 1487
Hin6I GCGC 3 cut(s) 1189, 1261, 1878
HinP1I GCGC 3 cut(s) 1189, 1261, 1878
HincII GTYRAC 2 cut(s) 747, 1215
HindII GTYRAC 2 cut(s) 747, 1215
HindIII AAGCTT 1 cut(s) 434
HpaII CCGG 4 cut(s) 17, 56, 1182, 1542
HphI GGTGA 6 cut(s) 182, 416, 1183, 1262, 1283, 1729
Hpy166II GTNNAC 2 cut(s) 747, 1215
Hpy188III TCNNGA 6 cut(s) 56, 224, 662, 1071, 1136, 1491
Hpy8I GTNNAC 2 cut(s) 747, 1215
HpyCH4III ACNGT 6 cut(s) 73, 669, 988, 1126, 1219, 1837
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 1589
HpyF3I CTNAG 6 cut(s) 65, 270, 349, 632, 700, 1240
Hsp92I GRCGYC 1 cut(s) 1487
HspAI GCGC 3 cut(s) 1189, 1261, 1878
Kpn2I TCCGGA 1 cut(s) 55
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
Kzo9I GATC 9 cut(s) 52, 117, 395, 406, 1039, 1176, 1497, 1585, 1766
LmnI GCTCC 3 cut(s) 664, 978, 1478
Lsp1109I GCAGC 1 cut(s) 231
LweI GCATC 3 cut(s) 231, 354, 700
MaeI CTAG 4 cut(s) 1434, 1449, 1604, 1881
MaeIII GTNAC 1 cut(s) 1228
MalI GATC 9 cut(s) 54, 119, 397, 408, 1041, 1178, 1499, 1587, 1768
MboI GATC 9 cut(s) 52, 117, 395, 406, 1039, 1176, 1497, 1585, 1766
MboII GAAGA 7 cut(s) 275, 794, 1037, 1186, 1295, 1557, 1776
MfeI CAATTG 3 cut(s) 399, 777, 1686
MflI RGATCY 5 cut(s) 52, 1039, 1176, 1497, 1585
MhlI GDGCHC 2 cut(s) 42, 692
MluCI AATT 7 cut(s) 150, 383, 399, 483, 616, 777, 1686
MlyI GAGTC 3 cut(s) 327, 1236, 1351
MroI TCCGGA 1 cut(s) 55
MroNI GCCGGC 1 cut(s) 16
MroXI GAANNNNTTC 1 cut(s) 1863
MseI TTAA 3 cut(s) 237, 455, 1854
MslI CAYNNNNRTG 2 cut(s) 377, 1715
MspA1I CMGCKG 1 cut(s) 1592
MspI CCGG 4 cut(s) 17, 56, 1182, 1542
MspR9I CCNGG 6 cut(s) 560, 715, 1169, 1182, 1542, 1626
MunI CAATTG 3 cut(s) 399, 777, 1686
MvaI CCWGG 4 cut(s) 560, 715, 1169, 1626
MwoI GCNNNNNNNGC 2 cut(s) 17, 1589
NaeI GCCGGC 1 cut(s) 18
NciI CCSGG 2 cut(s) 1182, 1542
NcoI CCATGG 2 cut(s) 113, 761
NdeII GATC 9 cut(s) 52, 117, 395, 406, 1039, 1176, 1497, 1585, 1766
NgoMIV GCCGGC 1 cut(s) 16
NlaIV GGNNCC 7 cut(s) 163, 643, 660, 675, 689, 690, 1587
NmuCI GTSAC 1 cut(s) 1228
NsbI TGCGCA 1 cut(s) 1190
NspI RCATGY 2 cut(s) 923, 1623
NspV TTCGAA 1 cut(s) 1514
PagI TCATGA 1 cut(s) 223
PceI AGGCCT 1 cut(s) 1678
PciI ACATGT 1 cut(s) 1619
PdiI GCCGGC 1 cut(s) 18
PdmI GAANNNNTTC 1 cut(s) 1863
PfeI GAWTC 7 cut(s) 128, 508, 583, 1145, 1397, 1565, 1825
PflMI CCANNNNNTGG 1 cut(s) 767
PfoI TCCNGGA 2 cut(s) 1540, 1624
PkrI GCNGC 2 cut(s) 246, 836
PleI GAGTC 3 cut(s) 326, 1235, 1351
PpsI GAGTC 3 cut(s) 326, 1235, 1351
PscI ACATGT 1 cut(s) 1619
PsiI TTATAA 1 cut(s) 863
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 4 cut(s) 558, 713, 1167, 1624
PspGI CCWGG 4 cut(s) 558, 713, 1167, 1624
PspN4I GGNNCC 7 cut(s) 163, 643, 660, 675, 689, 690, 1587
PspOMI GGGCCC 1 cut(s) 688
PspPI GGNCC 5 cut(s) 58, 161, 565, 688, 689
PsrI GAACNNNNNNTAC 2 cut(s) 128, 160
PstI CTGCAG 3 cut(s) 205, 850, 1876
PstNI CAGNNNCTG 1 cut(s) 992
PsuI RGATCY 5 cut(s) 52, 1039, 1176, 1497, 1585
PvuII CAGCTG 1 cut(s) 1592
RsaI GTAC 2 cut(s) 985, 1554
RsaNI GTAC 2 cut(s) 984, 1553
RseI CAYNNNNRTG 2 cut(s) 377, 1715
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 3 cut(s) 237, 455, 1854
SatI GCNGC 2 cut(s) 245, 835
Sau3AI GATC 9 cut(s) 52, 117, 395, 406, 1039, 1176, 1497, 1585, 1766
Sau96I GGNCC 5 cut(s) 58, 161, 565, 688, 689
SchI GAGTC 3 cut(s) 327, 1236, 1351
ScrFI CCNGG 6 cut(s) 560, 715, 1169, 1182, 1542, 1626
SduI GDGCHC 2 cut(s) 42, 692
SfaNI GCATC 3 cut(s) 231, 354, 700
SfcI CTRYAG 4 cut(s) 72, 201, 846, 1872
SfuI TTCGAA 1 cut(s) 1514
SinI GGWCC 2 cut(s) 58, 565
SmiMI CAYNNNNRTG 2 cut(s) 377, 1715
SmlI CTYRAG 2 cut(s) 1069, 1098
SmoI CTYRAG 2 cut(s) 1069, 1098
SpeI ACTAGT 1 cut(s) 1603
Sse9I AATT 7 cut(s) 150, 383, 399, 483, 616, 777, 1686
SseBI AGGCCT 1 cut(s) 1678
SsiI CCGC 8 cut(s) 313, 429, 563, 834, 889, 1008, 1583, 1812
SspI AATATT 1 cut(s) 1846
SspMI CTAG 4 cut(s) 1434, 1449, 1604, 1881
SstI GAGCTC 1 cut(s) 42
StuI AGGCCT 1 cut(s) 1678
StyD4I CCNGG 6 cut(s) 558, 713, 1167, 1180, 1540, 1624
StyI CCWWGG 3 cut(s) 113, 568, 761
TaaI ACNGT 6 cut(s) 73, 669, 988, 1126, 1219, 1837
TaqI TCGA 5 cut(s) 409, 1310, 1514, 1653, 1762
TasI AATT 7 cut(s) 150, 383, 399, 483, 616, 777, 1686
TatI WGTACW 2 cut(s) 983, 1552
TauI GCSGC 1 cut(s) 837
TfiI GAWTC 7 cut(s) 128, 508, 583, 1145, 1397, 1565, 1825
Tru1I TTAA 3 cut(s) 237, 455, 1854
Tru9I TTAA 3 cut(s) 237, 455, 1854
TscAI CASTG 5 cut(s) 274, 674, 1251, 1670, 1677
TseFI GTSAC 1 cut(s) 1228
TseI GCWGC 1 cut(s) 244
Tsp45I GTSAC 1 cut(s) 1228
TspDTI ATGAA 8 cut(s) 395, 437, 564, 597, 1295, 1389, 1578, 1727
TspGWI ACGGA 3 cut(s) 576, 722, 1789
TspRI CASTG 5 cut(s) 274, 674, 1251, 1670, 1677
Van91I CCANNNNNTGG 1 cut(s) 767
VpaK11BI GGWCC 2 cut(s) 58, 565
XagI CCTNNNNNAGG 1 cut(s) 1872
XapI RAATTY 2 cut(s) 383, 483
XceI RCATGY 2 cut(s) 923, 1623
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XmnI GAANNNNTTC 1 cut(s) 1863
XspI CTAG 4 cut(s) 1434, 1449, 1604, 1881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.