Rh4CG052500

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001247
Physical Location & Seq
Forward (+)
10402758 .. 10424477
21720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG052500.1

Sequence Viewer

Length: 1053 bp
ATGGAGCAGCAAATTGCAAAAGAAAAGCCTGCAATTTCTTTCGAGTATGAGCTCTTTCAAGACCCTGATCATCTAACAACTGTTGTAGCTACACTCACTCCAACTAGTCCCTGGATCAATCCTGCCTCATTGAACTTTAAGTACCGAATTGGACGGGGCCCCTTTGGTGATGTATGGTTAGCAACTCATCACCAGTCAGCAGATGATTATGATGAATATCATGAGGTAGCAGTTAAGATGTTGCATCCTTTGATGGAGGAGGACAGAGAAAAGTTTCTGCACAAGTTCGAAAAGTTATTTTTCAAGTTCCGCAGACTGTGCGGTGTTTGTTGGTTTCATGGTATCTCAATCATTGATGGAAGGATCTGCATCGCTATGAAATTTTATGAGGGGTCAGTTGGTGACCGAGTTGCTAGGTTAAAGGGTGGAAAACTTCAACTTTTTGATGTTTTAAGGTATGGGATTGAGTTGGCAAAAGGAATTTCAGAATTGCATTCACTAGGGCTTCTGGTGCTAAATTTGAAGCCGTCCAACATCCTTGTAGATGAACACGGCCAGGTGGTCCTTGGAGATTTTGGAATCCCATTTCTACTTCTTGGGGCTTCATTGTCTAATCCTAATATGGCTTTTAGACTTGGCACTCCGAACTACATGGCTCCAGAACAGTGGGAACCAAAAATATTGAAGTTCAAACCAAATGTTGTGATTACAGAAAAGGGGCTCAGTGACCTGGCATGCCATTATCTGAGCAAGGCCGGTGTTACTGCAATTAGGAGGTTGAGAAAAACAGATAATAACAGAATTGCCAAGGCTTGTGGGGCAGTTATTGTCAACAGACCCGATCAACTGCAAGAATCTGATATTGGTACTGGGGCTGGATTATTTGAGGTTAAAAAAATTGGAGATGAGTTCTTTACATTCATCGTTGATTGCAAAGATCCCAAGGCATGTACAGTACTCTTAAGAGGTCCGAGTAAGGATATGTTAAACGAAGTGGAAAGAAATTTACAGTATAAATTTTATCGGCCACGTCAGTATATTGATGCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1 Pkinase Pkinase_Tyr

Protein Analysis

350

Amino Acids

39.71

Weight (kDa)

8.07

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 45 - 226 5.8e-16 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 47 - 222 8.3e-15 Protein kinase domain
Cpn60_TCP1 PF00118 226 - 337 1.8e-30 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 310, 321
AclWI GGATC 3 cut(s) 122, 371, 932
AcoI YGGCCR 2 cut(s) 553, 1025
AcsI RAATTY 5 cut(s) 380, 480, 517, 1003, 1016
AfaI GTAC 4 cut(s) 143, 868, 952, 957
AflII CTTAAG 1 cut(s) 961
AgsI TTSAA 7 cut(s) 59, 133, 304, 437, 523, 685, 691
AhlI ACTAGT 1 cut(s) 104
AjiI CACGTC 1 cut(s) 1031
AjnI CCWGG 3 cut(s) 110, 555, 729
AjuI GAANNNNNNNTTGG 2 cut(s) 846, 878
AluBI AGCT 2 cut(s) 52, 89
AluI AGCT 2 cut(s) 52, 89
Alw21I GWGCWC 1 cut(s) 54
AlwI GGATC 3 cut(s) 122, 371, 932
AoxI GGCC 4 cut(s) 157, 553, 753, 1025
ApaI GGGCCC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 7
ApoI RAATTY 5 cut(s) 380, 480, 517, 1003, 1016
Asp700I GAANNNNTTC 1 cut(s) 273
AspS9I GGNCC 4 cut(s) 157, 158, 562, 968
AsuHPI GGTGA 3 cut(s) 179, 182, 413
AsuII TTCGAA 1 cut(s) 288
AvaII GGWCC 2 cut(s) 562, 968
BaeGI GKGCMC 1 cut(s) 161
BanII GRGCYC 3 cut(s) 54, 161, 723
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 1 cut(s) 19
BccI CCATC 2 cut(s) 247, 350
BceAI ACGGC 2 cut(s) 511, 568
BciT130I CCWGG 3 cut(s) 112, 557, 731
BclI TGATCA 1 cut(s) 67
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 3 cut(s) 105, 414, 500
BfrI CTTAAG 1 cut(s) 961
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
BmcAI AGTACT 1 cut(s) 957
Bme1390I CCNGG 3 cut(s) 112, 557, 731
Bme18I GGWCC 2 cut(s) 562, 968
BmgBI CACGTC 1 cut(s) 1031
BmgT120I GGNCC 4 cut(s) 157, 158, 562, 968
BmiI GGNNCC 5 cut(s) 158, 159, 160, 657, 672
BmrFI CCNGG 3 cut(s) 112, 557, 731
BmrI ACTGGG 1 cut(s) 879
BmsI GCATC 3 cut(s) 253, 378, 1033
BmuI ACTGGG 1 cut(s) 879
BpmI CTGGAG 1 cut(s) 642
Bpu14I TTCGAA 1 cut(s) 288
BsaBI GATNNNNATC 2 cut(s) 216, 368
BsaJI CCNNGG 4 cut(s) 110, 565, 807, 942
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 2 cut(s) 193, 874
Bse8I GATNNNNATC 2 cut(s) 216, 368
BseBI CCWGG 3 cut(s) 112, 557, 731
BseDI CCNNGG 4 cut(s) 110, 565, 807, 942
BseGI GGATG 2 cut(s) 244, 534
BseJI GATNNNNATC 2 cut(s) 216, 368
BseMII CTCAG 2 cut(s) 736, 737
BseNI ACTGG 2 cut(s) 193, 874
BseRI GAGGAG 1 cut(s) 272
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 263
BshFI GGCC 4 cut(s) 159, 555, 755, 1027
BsiHKAI GWGCWC 1 cut(s) 54
BsiSI CCGG 1 cut(s) 756
BslFI GGGAC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 93
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 4 cut(s) 159, 555, 755, 1027
Bsp119I TTCGAA 1 cut(s) 288
Bsp120I GGGCCC 1 cut(s) 157
Bsp1286I GDGCHC 3 cut(s) 54, 161, 723
Bsp1407I TGTACA 1 cut(s) 950
Bsp143I GATC 5 cut(s) 67, 114, 363, 841, 937
BspACI CCGC 2 cut(s) 310, 321
BspANI GGCC 4 cut(s) 159, 555, 755, 1027
BspCNI CTCAG 2 cut(s) 735, 738
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 5 cut(s) 158, 159, 160, 657, 672
BspPI GGATC 3 cut(s) 122, 371, 932
BspT104I TTCGAA 1 cut(s) 288
BspTI CTTAAG 1 cut(s) 961
BsrFI RCCGGY 1 cut(s) 755
BsrGI TGTACA 1 cut(s) 950
BsrI ACTGG 2 cut(s) 193, 874
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 4 cut(s) 110, 565, 807, 942
BssMI GATC 5 cut(s) 67, 114, 363, 841, 937
BssT1I CCWWGG 3 cut(s) 565, 807, 942
Bst2UI CCWGG 3 cut(s) 112, 557, 731
Bst4CI ACNGT 5 cut(s) 82, 318, 666, 955, 1011
BstAFI CTTAAG 1 cut(s) 961
BstAPI GCANNNNNTGC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 950
BstBI TTCGAA 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 30, 736
BstDEI CTNAG 2 cut(s) 722, 746
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 2 cut(s) 244, 534
BstKTI GATC 5 cut(s) 70, 117, 366, 844, 940
BstMBI GATC 5 cut(s) 67, 114, 363, 841, 937
BstMWI GCNNNNNNNGC 3 cut(s) 318, 511, 818
BstNI CCWGG 3 cut(s) 112, 557, 731
BstNSI RCATGY 2 cut(s) 738, 951
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 3 cut(s) 110, 555, 729
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 19
BstX2I RGATCY 2 cut(s) 363, 937
BstXI CCANNNNNNTGG 1 cut(s) 666
BstYI RGATCY 2 cut(s) 363, 937
BsuRI GGCC 4 cut(s) 159, 555, 755, 1027
BtgZI GCGATG 1 cut(s) 355
BtrI CACGTC 1 cut(s) 1031
BtsCI GGATG 2 cut(s) 244, 534
BtsIMutI CAGTG 2 cut(s) 671, 730
Cac8I GCNNGC 2 cut(s) 30, 736
CciI TCATGA 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 4 cut(s) 157, 158, 562, 968
Csp6I GTAC 4 cut(s) 142, 867, 951, 956
CspCI CAANNNNNGTGG 2 cut(s) 796, 831
CviAII CATG 5 cut(s) 221, 338, 652, 735, 948
CviQI GTAC 4 cut(s) 142, 867, 951, 956
DdeI CTNAG 2 cut(s) 722, 746
DpnI GATC 5 cut(s) 69, 116, 365, 843, 939
DpnII GATC 5 cut(s) 67, 114, 363, 841, 937
EaeI YGGCCR 2 cut(s) 553, 1025
Ecl136II GAGCTC 1 cut(s) 52
Eco130I CCWWGG 3 cut(s) 565, 807, 942
Eco24I GRGCYC 3 cut(s) 54, 161, 723
Eco47I GGWCC 2 cut(s) 562, 968
Eco53kI GAGCTC 1 cut(s) 52
Eco91I GGTNACC 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 52
EcoO109I RGGNCCY 2 cut(s) 157, 158
EcoO65I GGTNACC 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 110, 555, 729
EcoT14I CCWWGG 3 cut(s) 565, 807, 942
EcoT38I GRGCYC 3 cut(s) 54, 161, 723
ErhI CCWWGG 3 cut(s) 565, 807, 942
FaeI CATG 5 cut(s) 224, 341, 655, 738, 951
FaqI GGGAC 1 cut(s) 93
FatI CATG 5 cut(s) 220, 337, 651, 734, 947
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 1 cut(s) 8
FokI GGATG 2 cut(s) 231, 521
FriOI GRGCYC 3 cut(s) 54, 161, 723
Fsp4HI GCNGC 1 cut(s) 8
FspBI CTAG 3 cut(s) 105, 414, 500
GluI GCNGC 1 cut(s) 8
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 4 cut(s) 159, 555, 755, 1027
HapII CCGG 1 cut(s) 756
Hin1II CATG 5 cut(s) 224, 341, 655, 738, 951
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HinfI GANTC 2 cut(s) 579, 854
HpaII CCGG 1 cut(s) 756
HphI GGTGA 3 cut(s) 179, 182, 413
Hpy166II GTNNAC 1 cut(s) 832
Hpy188I TCNGA 5 cut(s) 487, 645, 747, 859, 972
Hpy188III TCNNGA 3 cut(s) 59, 221, 659
Hpy8I GTNNAC 1 cut(s) 832
HpyAV CCTTC 1 cut(s) 354
HpyCH4III ACNGT 5 cut(s) 82, 318, 666, 955, 1011
HpyCH4IV ACGT 1 cut(s) 1030
HpyF10VI GCNNNNNNNGC 3 cut(s) 318, 511, 818
HpyF3I CTNAG 2 cut(s) 722, 746
HpySE526I ACGT 1 cut(s) 1030
Hsp92II CATG 5 cut(s) 224, 341, 655, 738, 951
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 5 cut(s) 67, 114, 363, 841, 937
LmnI GCTCC 2 cut(s) 4, 661
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 3 cut(s) 253, 378, 1033
MaeI CTAG 3 cut(s) 105, 414, 500
MaeII ACGT 1 cut(s) 1030
MaeIII GTNAC 3 cut(s) 401, 725, 760
MalI GATC 5 cut(s) 69, 116, 365, 843, 939
MboI GATC 5 cut(s) 67, 114, 363, 841, 937
MflI RGATCY 2 cut(s) 363, 937
MhlI GDGCHC 3 cut(s) 54, 161, 723
MmeI TCCRAC 2 cut(s) 125, 555
MnlI CCTC 8 cut(s) 136, 217, 250, 253, 382, 768, 880, 959
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 7 cut(s) 138, 234, 419, 452, 891, 962, 986
MslI CAYNNNNRTG 1 cut(s) 374
MspCI CTTAAG 1 cut(s) 961
MspI CCGG 1 cut(s) 756
MspR9I CCNGG 3 cut(s) 112, 557, 731
Mva1269I GAATGC 1 cut(s) 493
MvaI CCWGG 3 cut(s) 112, 557, 731
MwoI GCNNNNNNNGC 3 cut(s) 318, 511, 818
NdeII GATC 5 cut(s) 67, 114, 363, 841, 937
NlaIII CATG 5 cut(s) 224, 341, 655, 738, 951
NlaIV GGNNCC 5 cut(s) 158, 159, 160, 657, 672
NmuCI GTSAC 2 cut(s) 401, 725
NspI RCATGY 2 cut(s) 738, 951
NspV TTCGAA 1 cut(s) 288
PaeI GCATGC 1 cut(s) 738
PagI TCATGA 1 cut(s) 220
PctI GAATGC 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 2 cut(s) 579, 854
PkrI GCNGC 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 54
Psp6I CCWGG 3 cut(s) 110, 555, 729
PspEI GGTNACC 1 cut(s) 401
PspGI CCWGG 3 cut(s) 110, 555, 729
PspN4I GGNNCC 5 cut(s) 158, 159, 160, 657, 672
PspOMI GGGCCC 1 cut(s) 157
PspPI GGNCC 4 cut(s) 157, 158, 562, 968
PsrI GAACNNNNNNTAC 2 cut(s) 125, 157
PsuI RGATCY 2 cut(s) 363, 937
RsaI GTAC 4 cut(s) 143, 868, 952, 957
RsaNI GTAC 4 cut(s) 142, 867, 951, 956
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 1 cut(s) 54
SaqAI TTAA 7 cut(s) 138, 234, 419, 452, 891, 962, 986
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 5 cut(s) 67, 114, 363, 841, 937
Sau96I GGNCC 4 cut(s) 157, 158, 562, 968
ScaI AGTACT 1 cut(s) 957
ScrFI CCNGG 3 cut(s) 112, 557, 731
SduI GDGCHC 3 cut(s) 54, 161, 723
SfaNI GCATC 3 cut(s) 253, 378, 1033
SfuI TTCGAA 1 cut(s) 288
SinI GGWCC 2 cut(s) 562, 968
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 961
SmoI CTYRAG 1 cut(s) 961
SpeI ACTAGT 1 cut(s) 104
SphI GCATGC 1 cut(s) 738
SsiI CCGC 2 cut(s) 310, 321
SspI AATATT 1 cut(s) 681
SspMI CTAG 3 cut(s) 105, 414, 500
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 3 cut(s) 110, 555, 729
StyI CCWWGG 3 cut(s) 565, 807, 942
TaaI ACNGT 5 cut(s) 82, 318, 666, 955, 1011
TaiI ACGT 1 cut(s) 1033
TaqI TCGA 2 cut(s) 42, 288
TaqII GACCGA 1 cut(s) 420
TatI WGTACW 2 cut(s) 950, 955
TfiI GAWTC 2 cut(s) 579, 854
Tru1I TTAA 7 cut(s) 138, 234, 419, 452, 891, 962, 986
Tru9I TTAA 7 cut(s) 138, 234, 419, 452, 891, 962, 986
TscAI CASTG 2 cut(s) 671, 730
TseFI GTSAC 2 cut(s) 401, 725
TseI GCWGC 1 cut(s) 7
Tsp45I GTSAC 2 cut(s) 401, 725
TspDTI ATGAA 6 cut(s) 228, 326, 392, 561, 594, 910
TspRI CASTG 2 cut(s) 671, 730
Vha464I CTTAAG 1 cut(s) 961
VpaK11BI GGWCC 2 cut(s) 562, 968
XapI RAATTY 5 cut(s) 380, 480, 517, 1003, 1016
XceI RCATGY 2 cut(s) 738, 951
XcmI CCANNNNNNNNNTGG 2 cut(s) 108, 563
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 3 cut(s) 105, 414, 500
ZrmI AGTACT 1 cut(s) 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.