Rh2DG396300

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001247
Physical Location & Seq
Reverse (-)
57653159 .. 57656855
3697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG396300.1

Sequence Viewer

Length: 1932 bp
ATGTGGCTATCAAATTCGGCCAGTTCGAGTTTCGCTCCCGGACGTCACAGAGCTTCTGTTTCTCTCCGCAGCAAAATGGAGCAGCAAATTGCAAAAGAAAAGCCTGCAATTGCTTTCGAGTATGAGCTCTTTGAAGACCCTGATCATCTAACAACTGTTGTAGCTACACTCACTCCAACTAGTCCCTGGATCAATCCTGCCTCATTGAACTTTAAGTACCGAATTGGACGAGGCCCCTTTGGTGATGTATGGTTAGCAACTCATCATCAGTCAGCGGATGATTATGATGAATATCATGAGGTAGCGGTTAAGATGTTGCAACCTTTGACGGAGGAGGACAGAGAAAAGTTTCTGCACAAGTTCGAAAAGTTATTTTTCAAGTTCCGCAGACTGTGCGGTGTTTGTTGGTTTCATGGTATCTCAATCATTGATGGAAGGATCTGTATCGCTATGAAATTTTATGAGGGGTCAGTTGGTGACCGAGTTGCTAGGTTGAAGGGTGGAAAACTTCAACTTTTTGATGTTTTGAGGTATGGGATTGAGTTGGCAAAAGGAATTTCAGAATTGCATTCACTAGGGCTTCTGGTGCTAAATTTGAAGCCGTCCAACATCCTTGTAGATGAACACGGCCAGGTGGTCCTTGGAGATTTTGGAATCCCATTTCTACTTCTTGGGGCTTCATTGTCTAATCCTAATATGTCTTTTAGACTTGGCACTCCGAACTACATGGCTCCAGAACAGTGGGAACCAGAAGTGAGGGGTCCTATATCTTTAGAAACAGATTCTTGGGGCTTTGGATGTTGCTTTGTAGAGATGTTGACCGGTAGTCAACCCTGGTTTGGGAGTTCAATTAAAGAAATATATCATTCAGTTGTGATCAAGCGAGAAAAGCCACCTGTTCCAAGTGGTTTGCCTCCTGCAGTGGAGAATGTTATCAACGGCTGCTTTGAGTATGATCTCCGTAATAGGCCTTCAATGGAAGATATCATACGTGCATTGGAAAGCTCACAAAAAGATGTTCAAAGCGATGGAGAATGGCTTGGTTTAGGGAGCAGCACACCTGCTGGAAAACTTAGTAGCGGCAGTTATAGTGCGTGGCATCTCTTAAAAGATGATCTCCAGGTTGGAGACATAGTTCGCTCCAGAAAGCCAGTAAATGCACGCAAGCGTCAAGCTCTTGATGTCCCTGAAGGAATTGTAACTGGCCTGGAAAGTGATTCTTGTAGTGCCCTGGTGAAGATCCCTCGAGCGCGCAAGCCTGTCAGAGTATCAGTGTCAACACTTGAGAGAGTCACATCTGGCTTGACAGCAGGTGATTGGGTGCGGTTGAAGGGAGAACTGCACAAGCACTCTCCTGTTGGAATTCTTCATTCAATACAACGTGATGGAAGTGTTGCTGTTGGATTTATTGGGCTAGAGACTCTATGGAGAGGGAAATCATCTGACATTGAAATGGCTGATGCATATTATGTAGGAGAGTTTGTGAGGCTGAAGGCGAATGTGGTTTCTCCCAGATTTGAGTGGCCTCGGAAGGGTGGAGGTGGGGCATGGGCTACTGGAAGGATCTCACAAGTCCTTCCAAATGGTTGTCTGGTTGTGAGGTTCCCTGGAAGATTGGAATTTGGAGATGAAGCTCGTAGCTTCTTGGCAGATCCATATGAAGTAGAACTAGTGTCCTTTGATACTTGTCCTGGTCTGGTCGAAAAATATCAACATATTGAGGATTTTCACTGGGCAGTCAGGCCACTAACAATTGCATTTGGTCTCTTTACAGCCATGAAGTTCAGCTTGTTTGTTGGACGAAATGTAAGCGCAAGGGTGAAAGGCCGAAGAAACTCACTACAGGGCCAGAGCGGTGGAACTTCAGCTTGGCTTCCTCCGCCGGTTGCAAATCTTTTCAAAGAAGGTTCTTCTACTGCTACTGCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1 Pkinase Pkinase_Tyr

Protein Analysis

643

Amino Acids

71.3

Weight (kDa)

8.24

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 70 - 333 1.1e-31 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 72 - 330 4.5e-28 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1069, 1301
AatII GACGTC 1 cut(s) 46
Acc36I ACCTGC 2 cut(s) 1069, 1301
AccBSI CCGCTC 1 cut(s) 1854
AccII CGCG 1 cut(s) 1252
AciI CCGC 9 cut(s) 67, 275, 305, 385, 396, 1080, 1324, 1854, 1880
AclWI GGATC 5 cut(s) 197, 446, 1234, 1571, 1646
AcoI YGGCCR 2 cut(s) 18, 628
AcsI RAATTY 6 cut(s) 13, 455, 555, 592, 1362, 1619
AcuI CTGAAG 3 cut(s) 1209, 1511, 1848
AcyI GRCGYC 1 cut(s) 43
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 3 cut(s) 839, 840, 1532
AgeI ACCGGT 1 cut(s) 821
AhdI GACNNNNNGTC 1 cut(s) 825
AhlI ACTAGT 2 cut(s) 179, 1669
AjnI CCWGG 8 cut(s) 185, 630, 833, 1119, 1206, 1230, 1606, 1690
AjuI GAANNNNNNNTTGG 2 cut(s) 1852, 1884
AloI GAACNNNNNNTCC 2 cut(s) 1119, 1151
AluBI AGCT 9 cut(s) 53, 127, 164, 1005, 1175, 1634, 1641, 1788, 1868
AluI AGCT 9 cut(s) 53, 127, 164, 1005, 1175, 1634, 1641, 1788, 1868
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 3 cut(s) 1122, 1412, 1769
AlwI GGATC 5 cut(s) 197, 446, 1234, 1571, 1646
Ama87I CYCGRG 1 cut(s) 1245
AoxI GGCC 9 cut(s) 18, 232, 628, 968, 1204, 1523, 1742, 1825, 1846
ApeKI GCWGC 4 cut(s) 69, 82, 942, 1053
ApoI RAATTY 6 cut(s) 13, 455, 555, 592, 1362, 1619
AsiGI ACCGGT 1 cut(s) 821
Asp700I GAANNNNTTC 1 cut(s) 348
AspLEI GCGC 3 cut(s) 1252, 1254, 1814
AspS9I GGNCC 4 cut(s) 233, 637, 761, 1846
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 5 cut(s) 254, 488, 1246, 1325, 1831
AsuII TTCGAA 1 cut(s) 363
AvaI CYCGRG 1 cut(s) 1245
AvaII GGWCC 2 cut(s) 637, 761
BaeGI GKGCMC 1 cut(s) 1231
BanII GRGCYC 1 cut(s) 129
BarI GAAGNNNNNNTAC 4 cut(s) 955, 972, 987, 1004
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 4 cut(s) 81, 94, 929, 1065
BccI CCATC 3 cut(s) 425, 1022, 1379
BceAI ACGGC 3 cut(s) 586, 643, 955
BciT130I CCWGG 8 cut(s) 187, 632, 835, 1121, 1208, 1232, 1608, 1692
BclI TGATCA 2 cut(s) 142, 876
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 3 cut(s) 1122, 1412, 1769
BcuI ACTAGT 2 cut(s) 179, 1669
BfaI CTAG 6 cut(s) 180, 489, 575, 1415, 1670, 1926
BfmI CTRYAG 2 cut(s) 918, 1841
BfuAI ACCTGC 2 cut(s) 1069, 1301
BisI GCNGC 5 cut(s) 70, 83, 943, 1054, 1081
BlsI GCNGC 5 cut(s) 71, 84, 944, 1055, 1082
Bme1390I CCNGG 9 cut(s) 39, 187, 632, 835, 1121, 1208, 1232, 1608, 1692
Bme18I GGWCC 2 cut(s) 637, 761
BmeRI GACNNNNNGTC 1 cut(s) 825
BmeT110I CYCGRG 1 cut(s) 1245
BmgT120I GGNCC 4 cut(s) 233, 637, 761, 1846
BmiI GGNNCC 5 cut(s) 235, 732, 747, 762, 1604
BmrFI CCNGG 9 cut(s) 39, 187, 632, 835, 1121, 1208, 1232, 1608, 1692
BmrI ACTGGG 1 cut(s) 1741
BmsI GCATC 2 cut(s) 1108, 1450
BmuI ACTGGG 1 cut(s) 1741
BpiI GAAGAC 1 cut(s) 141
BplI GAGNNNNNCTC 2 cut(s) 19, 51
BpmI CTGGAG 3 cut(s) 717, 1103, 1126
Bpu14I TTCGAA 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 1304
BpuMI CCSGG 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 992
BsaBI GATNNNNATC 2 cut(s) 291, 443
BsaHI GRCGYC 1 cut(s) 43
BsaI GGTCTC 1 cut(s) 1769
BsaJI CCNNGG 6 cut(s) 185, 640, 833, 1230, 1526, 1606
BsaWI WCCGGW 1 cut(s) 821
BsaXI ACNNNNNCTCC 6 cut(s) 157, 187, 1119, 1149, 1467, 1497
Bsc4I CCNNNNNNNGG 3 cut(s) 839, 840, 1532
Bse118I RCCGGY 2 cut(s) 821, 1882
Bse1I ACTGG 5 cut(s) 21, 1151, 1207, 1561, 1736
Bse8I GATNNNNATC 2 cut(s) 291, 443
BseBI CCWGG 8 cut(s) 187, 632, 835, 1121, 1208, 1232, 1608, 1692
BseDI CCNNGG 6 cut(s) 185, 640, 833, 1230, 1526, 1606
BseGI GGATG 3 cut(s) 283, 609, 803
BseJI GATNNNNATC 2 cut(s) 291, 443
BseLI CCNNNNNNNGG 3 cut(s) 839, 840, 1532
BseNI ACTGG 5 cut(s) 21, 1151, 1207, 1561, 1736
BsePI GCGCGC 1 cut(s) 1250
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 1231
BseXI GCAGC 4 cut(s) 81, 94, 929, 1065
BsgI GTGCAG 2 cut(s) 338, 1325
Bsh1236I CGCG 1 cut(s) 1252
BshFI GGCC 9 cut(s) 20, 234, 630, 970, 1206, 1525, 1744, 1827, 1848
BshTI ACCGGT 1 cut(s) 821
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 1245
BsiSI CCGG 3 cut(s) 39, 822, 1883
BslFI GGGAC 2 cut(s) 168, 1169
BslI CCNNNNNNNGG 3 cut(s) 839, 840, 1532
BsmAI GTCTC 3 cut(s) 1122, 1412, 1769
BsmFI GGGAC 2 cut(s) 168, 1169
BsmI GAATGC 1 cut(s) 568
BsnI GGCC 9 cut(s) 20, 234, 630, 970, 1206, 1525, 1744, 1827, 1848
Bso31I GGTCTC 1 cut(s) 1769
BsoBI CYCGRG 1 cut(s) 1245
Bsp119I TTCGAA 1 cut(s) 363
Bsp1286I GDGCHC 2 cut(s) 129, 1231
Bsp143I GATC 9 cut(s) 142, 189, 438, 876, 955, 1114, 1239, 1563, 1651
BspACI CCGC 9 cut(s) 67, 275, 305, 385, 396, 1080, 1324, 1854, 1880
BspANI GGCC 9 cut(s) 20, 234, 630, 970, 1206, 1525, 1744, 1827, 1848
BspFNI CGCG 1 cut(s) 1252
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 5 cut(s) 235, 732, 747, 762, 1604
BspMAI CTGCAG 1 cut(s) 922
BspMI ACCTGC 2 cut(s) 1069, 1301
BspPI GGATC 5 cut(s) 197, 446, 1234, 1571, 1646
BspT104I TTCGAA 1 cut(s) 363
BspTNI GGTCTC 1 cut(s) 1769
BsrBI CCGCTC 1 cut(s) 1854
BsrFI RCCGGY 2 cut(s) 821, 1882
BsrI ACTGG 5 cut(s) 21, 1151, 1207, 1561, 1736
BssAI RCCGGY 2 cut(s) 821, 1882
BssECI CCNNGG 6 cut(s) 185, 640, 833, 1230, 1526, 1606
BssHII GCGCGC 1 cut(s) 1250
BssMI GATC 9 cut(s) 142, 189, 438, 876, 955, 1114, 1239, 1563, 1651
BssNI GRCGYC 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 640
Bst2UI CCWGG 8 cut(s) 187, 632, 835, 1121, 1208, 1232, 1608, 1692
Bst4CI ACNGT 3 cut(s) 157, 393, 741
BstACI GRCGYC 1 cut(s) 43
BstAPI GCANNNNNTGC 1 cut(s) 393
BstBAI YACGTR 1 cut(s) 992
BstBI TTCGAA 1 cut(s) 363
BstC8I GCNNGC 5 cut(s) 105, 1162, 1166, 1252, 1256
BstDEI CTNAG 1 cut(s) 1073
BstEII GGTNACC 1 cut(s) 476
BstF5I GGATG 3 cut(s) 283, 609, 803
BstFNI CGCG 1 cut(s) 1252
BstHHI GCGC 3 cut(s) 1252, 1254, 1814
BstKTI GATC 9 cut(s) 145, 192, 441, 879, 958, 1117, 1242, 1566, 1654
BstMAI GTCTC 3 cut(s) 1122, 1412, 1769
BstMBI GATC 9 cut(s) 142, 189, 438, 876, 955, 1114, 1239, 1563, 1651
BstMWI GCNNNNNNNGC 4 cut(s) 393, 586, 889, 1879
BstNI CCWGG 8 cut(s) 187, 632, 835, 1121, 1208, 1232, 1608, 1692
BstPI GGTNACC 1 cut(s) 476
BstSCI CCNGG 9 cut(s) 37, 185, 630, 833, 1119, 1206, 1230, 1606, 1690
BstSFI CTRYAG 2 cut(s) 918, 1841
BstSLI GKGCMC 1 cut(s) 1231
BstUI CGCG 1 cut(s) 1252
BstV1I GCAGC 4 cut(s) 81, 94, 929, 1065
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 4 cut(s) 438, 1239, 1563, 1651
BstXI CCANNNNNNTGG 2 cut(s) 741, 1856
BstYI RGATCY 4 cut(s) 438, 1239, 1563, 1651
BsuRI GGCC 9 cut(s) 20, 234, 630, 970, 1206, 1525, 1744, 1827, 1848
BtgZI GCGATG 1 cut(s) 1041
BtsCI GGATG 3 cut(s) 283, 609, 803
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 4 cut(s) 746, 927, 1278, 1729
BveI ACCTGC 2 cut(s) 1069, 1301
Cac8I GCNNGC 5 cut(s) 105, 1162, 1166, 1252, 1256
CciI TCATGA 1 cut(s) 295
CfoI GCGC 3 cut(s) 1252, 1254, 1814
Cfr10I RCCGGY 2 cut(s) 821, 1882
Cfr13I GGNCC 4 cut(s) 233, 637, 761, 1846
CseI GACGC 1 cut(s) 1157
Csp6I GTAC 1 cut(s) 217
CspAI ACCGGT 1 cut(s) 821
CviAII CATG 5 cut(s) 296, 413, 727, 1548, 1777
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 1 cut(s) 1073
DpnI GATC 9 cut(s) 144, 191, 440, 878, 957, 1116, 1241, 1565, 1653
DpnII GATC 9 cut(s) 142, 189, 438, 876, 955, 1114, 1239, 1563, 1651
DriI GACNNNNNGTC 1 cut(s) 825
EaeI YGGCCR 2 cut(s) 18, 628
Eam1105I GACNNNNNGTC 1 cut(s) 825
EciI GGCGGA 1 cut(s) 1869
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 640
Eco147I AGGCCT 1 cut(s) 970
Eco24I GRGCYC 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 1769
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 2 cut(s) 637, 761
Eco53kI GAGCTC 1 cut(s) 127
Eco57I CTGAAG 3 cut(s) 1209, 1511, 1848
Eco88I CYCGRG 1 cut(s) 1245
Eco91I GGTNACC 1 cut(s) 476
EcoICRI GAGCTC 1 cut(s) 127
EcoO109I RGGNCCY 2 cut(s) 233, 761
EcoO65I GGTNACC 1 cut(s) 476
EcoRI GAATTC 1 cut(s) 1362
EcoRII CCWGG 8 cut(s) 185, 630, 833, 1119, 1206, 1230, 1606, 1690
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 1 cut(s) 640
EcoT22I ATGCAT 1 cut(s) 1465
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 640
FaeI CATG 5 cut(s) 299, 416, 730, 1551, 1780
FalI AAGNNNNNCTT 4 cut(s) 1204, 1236, 1772, 1804
FaqI GGGAC 2 cut(s) 168, 1169
FatI CATG 5 cut(s) 295, 412, 726, 1547, 1776
FauNDI CATATG 1 cut(s) 1657
FbaI TGATCA 2 cut(s) 142, 876
Fnu4HI GCNGC 5 cut(s) 70, 83, 943, 1054, 1081
FokI GGATG 3 cut(s) 290, 596, 810
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 5 cut(s) 70, 83, 943, 1054, 1081
FspBI CTAG 6 cut(s) 180, 489, 575, 1415, 1670, 1926
GlaI GCGC 3 cut(s) 1251, 1253, 1813
GluI GCNGC 5 cut(s) 70, 83, 943, 1054, 1081
GsuI CTGGAG 3 cut(s) 717, 1103, 1126
HaeIII GGCC 9 cut(s) 20, 234, 630, 970, 1206, 1525, 1744, 1827, 1848
HapII CCGG 3 cut(s) 39, 822, 1883
HgaI GACGC 1 cut(s) 1157
HhaI GCGC 3 cut(s) 1252, 1254, 1814
Hin1I GRCGYC 1 cut(s) 43
Hin1II CATG 5 cut(s) 299, 416, 730, 1551, 1780
Hin6I GCGC 3 cut(s) 1250, 1252, 1812
HinP1I GCGC 3 cut(s) 1250, 1252, 1812
HincII GTYRAC 3 cut(s) 819, 830, 1278
HindII GTYRAC 3 cut(s) 819, 830, 1278
HinfI GANTC 5 cut(s) 654, 782, 1217, 1290, 1420
HpaII CCGG 3 cut(s) 39, 822, 1883
HphI GGTGA 5 cut(s) 254, 488, 1246, 1325, 1831
Hpy166II GTNNAC 3 cut(s) 819, 830, 1278
Hpy188I TCNGA 5 cut(s) 562, 720, 1265, 1444, 1530
Hpy188III TCNNGA 4 cut(s) 296, 734, 1143, 1178
Hpy8I GTNNAC 3 cut(s) 819, 830, 1278
HpyCH4III ACNGT 3 cut(s) 157, 393, 741
HpyCH4IV ACGT 3 cut(s) 43, 991, 1381
HpyF10VI GCNNNNNNNGC 4 cut(s) 393, 586, 889, 1879
HpyF3I CTNAG 1 cut(s) 1073
HpySE526I ACGT 3 cut(s) 43, 991, 1381
Hsp92I GRCGYC 1 cut(s) 43
Hsp92II CATG 5 cut(s) 299, 416, 730, 1551, 1780
HspAI GCGC 3 cut(s) 1250, 1252, 1812
Ksp22I TGATCA 2 cut(s) 142, 876
Kzo9I GATC 9 cut(s) 142, 189, 438, 876, 955, 1114, 1239, 1563, 1651
LmnI GCTCC 5 cut(s) 40, 79, 736, 1050, 1145
Lsp1109I GCAGC 4 cut(s) 81, 94, 929, 1065
LweI GCATC 2 cut(s) 1108, 1450
MaeI CTAG 6 cut(s) 180, 489, 575, 1415, 1670, 1926
MaeII ACGT 3 cut(s) 43, 991, 1381
MaeIII GTNAC 4 cut(s) 44, 476, 1198, 1291
MalI GATC 9 cut(s) 144, 191, 440, 878, 957, 1116, 1241, 1565, 1653
MbiI CCGCTC 1 cut(s) 1854
MboI GATC 9 cut(s) 142, 189, 438, 876, 955, 1114, 1239, 1563, 1651
MboII GAAGA 7 cut(s) 146, 992, 1249, 1358, 1623, 1842, 1902
MfeI CAATTG 2 cut(s) 108, 1752
MflI RGATCY 4 cut(s) 438, 1239, 1563, 1651
MhlI GDGCHC 2 cut(s) 129, 1231
MlyI GAGTC 2 cut(s) 1299, 1414
MmeI TCCRAC 6 cut(s) 200, 630, 1105, 1339, 1381, 1777
Mph1103I ATGCAT 1 cut(s) 1465
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 4 cut(s) 213, 309, 852, 1106
MslI CAYNNNNRTG 1 cut(s) 1451
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 3 cut(s) 39, 822, 1883
MspR9I CCNGG 9 cut(s) 39, 187, 632, 835, 1121, 1208, 1232, 1608, 1692
MunI CAATTG 2 cut(s) 108, 1752
Mva1269I GAATGC 1 cut(s) 568
MvaI CCWGG 8 cut(s) 187, 632, 835, 1121, 1208, 1232, 1608, 1692
MvnI CGCG 1 cut(s) 1252
MwoI GCNNNNNNNGC 4 cut(s) 393, 586, 889, 1879
NciI CCSGG 1 cut(s) 39
NdeI CATATG 1 cut(s) 1657
NdeII GATC 9 cut(s) 142, 189, 438, 876, 955, 1114, 1239, 1563, 1651
NlaIII CATG 5 cut(s) 299, 416, 730, 1551, 1780
NlaIV GGNNCC 5 cut(s) 235, 732, 747, 762, 1604
NmuCI GTSAC 3 cut(s) 44, 476, 1291
NsiI ATGCAT 1 cut(s) 1465
NspV TTCGAA 1 cut(s) 363
PaeR7I CTCGAG 1 cut(s) 1245
PagI TCATGA 1 cut(s) 295
PaqCI CACCTGC 2 cut(s) 1069, 1301
PauI GCGCGC 1 cut(s) 1250
PceI AGGCCT 1 cut(s) 970
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 1 cut(s) 568
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 654, 782, 1217
PfoI TCCNGGA 1 cut(s) 37
PinAI ACCGGT 1 cut(s) 821
PkrI GCNGC 5 cut(s) 71, 84, 944, 1055, 1082
PleI GAGTC 2 cut(s) 1298, 1414
PpsI GAGTC 2 cut(s) 1298, 1414
Ppu21I YACGTR 1 cut(s) 992
PpuMI RGGWCCY 1 cut(s) 761
Psp124BI GAGCTC 1 cut(s) 129
Psp5II RGGWCCY 1 cut(s) 761
Psp6I CCWGG 8 cut(s) 185, 630, 833, 1119, 1206, 1230, 1606, 1690
PspEI GGTNACC 1 cut(s) 476
PspGI CCWGG 8 cut(s) 185, 630, 833, 1119, 1206, 1230, 1606, 1690
PspN4I GGNNCC 5 cut(s) 235, 732, 747, 762, 1604
PspPI GGNCC 4 cut(s) 233, 637, 761, 1846
PspPPI RGGWCCY 1 cut(s) 761
PspXI VCTCGAGB 1 cut(s) 1245
PsrI GAACNNNNNNTAC 2 cut(s) 200, 232
PstI CTGCAG 1 cut(s) 922
PsuI RGATCY 4 cut(s) 438, 1239, 1563, 1651
PteI GCGCGC 1 cut(s) 1250
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
RseI CAYNNNNRTG 1 cut(s) 1451
SacI GAGCTC 1 cut(s) 129
SaqAI TTAA 4 cut(s) 213, 309, 852, 1106
SatI GCNGC 5 cut(s) 70, 83, 943, 1054, 1081
Sau3AI GATC 9 cut(s) 142, 189, 438, 876, 955, 1114, 1239, 1563, 1651
Sau96I GGNCC 4 cut(s) 233, 637, 761, 1846
SchI GAGTC 2 cut(s) 1299, 1414
ScrFI CCNGG 9 cut(s) 39, 187, 632, 835, 1121, 1208, 1232, 1608, 1692
SduI GDGCHC 2 cut(s) 129, 1231
SfaNI GCATC 2 cut(s) 1108, 1450
SfcI CTRYAG 2 cut(s) 918, 1841
Sfr274I CTCGAG 1 cut(s) 1245
SfuI TTCGAA 1 cut(s) 363
SinI GGWCC 2 cut(s) 637, 761
SlaI CTCGAG 1 cut(s) 1245
SmiMI CAYNNNNRTG 1 cut(s) 1451
SmlI CTYRAG 2 cut(s) 1245, 1283
SmoI CTYRAG 2 cut(s) 1245, 1283
SpeI ACTAGT 2 cut(s) 179, 1669
SseBI AGGCCT 1 cut(s) 970
SsiI CCGC 9 cut(s) 67, 275, 305, 385, 396, 1080, 1324, 1854, 1880
SspMI CTAG 6 cut(s) 180, 489, 575, 1415, 1670, 1926
SstI GAGCTC 1 cut(s) 129
StuI AGGCCT 1 cut(s) 970
StyD4I CCNGG 9 cut(s) 37, 185, 630, 833, 1119, 1206, 1230, 1606, 1690
StyI CCWWGG 1 cut(s) 640
TaaI ACNGT 3 cut(s) 157, 393, 741
TaiI ACGT 3 cut(s) 46, 994, 1384
TaqI TCGA 5 cut(s) 26, 117, 363, 1246, 1701
TaqII GACCGA 1 cut(s) 495
TauI GCSGC 1 cut(s) 1083
TfiI GAWTC 3 cut(s) 654, 782, 1217
Tru1I TTAA 4 cut(s) 213, 309, 852, 1106
Tru9I TTAA 4 cut(s) 213, 309, 852, 1106
TscAI CASTG 4 cut(s) 746, 927, 1278, 1736
TseFI GTSAC 3 cut(s) 44, 476, 1291
TseI GCWGC 4 cut(s) 69, 82, 942, 1053
Tsp45I GTSAC 3 cut(s) 44, 476, 1291
TspDTI ATGAA 9 cut(s) 303, 401, 467, 636, 669, 1358, 1644, 1674, 1793
TspGWI ACGGA 2 cut(s) 344, 950
TspRI CASTG 4 cut(s) 746, 927, 1278, 1736
VpaK11BI GGWCC 2 cut(s) 637, 761
XapI RAATTY 6 cut(s) 13, 455, 555, 592, 1362, 1619
XcmI CCANNNNNNNNNTGG 2 cut(s) 183, 638
XhoI CTCGAG 1 cut(s) 1245
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 6 cut(s) 180, 489, 575, 1415, 1670, 1926
ZraI GACGTC 1 cut(s) 44
Zsp2I ATGCAT 1 cut(s) 1465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.