Rmu_sc0024743.1_g000001

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0024743.1
OG0001247
Physical Location & Seq
Forward (+)
1 .. 2117
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0024743.1_g000001.1.cds

Sequence Viewer

Length: 1043 bp
agaatattcttcttggctgctttgagtatgacttacggagtcgtccttcaacaatggacatattgaatgctttcaaaagcttgcagaatgcagtcagtgacgtagactggacatatgttgggaatagagcaatcacagagaaatcgagtagcactggttacactgagtggtttctttcgaaggatcatctgctggtgggtgacatagttcgttccagaaagtcaccaaactcctgcaagcctgaaaacatggatgttccggaaggcacagtggtgggtatagaacatgatacagagcaacatgggtatgtcttggtgagggtccatggcatccatgacccattgagggttcatgcttcaactctggagcgggttacttttggcttggcagctggtgactgggtacgtttgaagaaggaagacaagaaacactcgccagtaggtattctccattccatcaatcgagacggtagtgtagctgttggatttatagggttggaaactctttggaagggaacttctttagagtttcagatggcagaatcttattgtgtgggtcaatttgttaggctgaaagccaatgttctgagtcctcgattcgaatggcctcgtaagacgggagggaaatgggctactggtaagatatggtggatcctaccaaatgggtgccttgttgtgaagttcccaggtatactgactttggggcatgaaaactcaacatttgtggctgatcctgcagaggtcgaatcagttacatttgagacttgtccaggaatggtgaagaagtaccaacatcttgaggattttcattgggcagtgaggccaattctgattgcattgggcctatttacagctatgaagatgggcatctgtattggtacaaaaattgggaagtgtaaagcgaagaaacgatggagtgcagctatacaggatgagagtcaccatttggatggtcagaattcaggaaatccagcttggaagaatccggtgacaaagatccttttcagagaaggtggcggttctgccacaggtcttgctcggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

346

Amino Acids

38.43

Weight (kDa)

8.86

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 665
AccBSI CCGCTC 1 cut(s) 369
AccI GTMKAC 2 cut(s) 104, 690
AccIII TCCGGA 1 cut(s) 258
AciI CCGC 2 cut(s) 369, 1016
AclWI GGATC 5 cut(s) 191, 645, 658, 724, 990
AcsI RAATTY 1 cut(s) 957
AdeI CACNNNGTG 1 cut(s) 167
AfaI GTAC 3 cut(s) 404, 787, 879
AfiI CCNNNNNNNGG 1 cut(s) 345
AgsI TTSAA 5 cut(s) 50, 66, 75, 359, 411
AjnI CCWGG 2 cut(s) 684, 768
AjuI GAANNNNNNNTTGG 2 cut(s) 957, 989
AleI CACNNNNGTG 1 cut(s) 271
AluBI AGCT 6 cut(s) 80, 391, 478, 853, 922, 973
AluI AGCT 6 cut(s) 80, 391, 478, 853, 922, 973
Alw26I GTCTC 2 cut(s) 458, 754
AlwI GGATC 5 cut(s) 191, 645, 658, 724, 990
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 3 cut(s) 604, 820, 840
ApeKI GCWGC 3 cut(s) 17, 388, 919
ApoI RAATTY 1 cut(s) 957
Asp700I GAANNNNTTC 1 cut(s) 70
AspS9I GGNCC 2 cut(s) 321, 840
AsuHPI GGTGA 7 cut(s) 211, 215, 327, 406, 789, 931, 999
AsuII TTCGAA 2 cut(s) 178, 599
AvaII GGWCC 1 cut(s) 321
BamHI GGATCC 1 cut(s) 650
BanI GGYRCC 1 cut(s) 665
BbsI GAAGAC 1 cut(s) 425
BbvI GCAGC 3 cut(s) 4, 400, 931
BccI CCATC 5 cut(s) 463, 528, 855, 905, 943
BciT130I CCWGG 2 cut(s) 686, 770
BcoDI GTCTC 2 cut(s) 458, 754
BfmI CTRYAG 1 cut(s) 734
BisI GCNGC 3 cut(s) 18, 389, 920
BlsI GCNGC 3 cut(s) 19, 390, 921
Bme1390I CCNGG 2 cut(s) 686, 770
Bme18I GGWCC 1 cut(s) 321
BmgT120I GGNCC 2 cut(s) 321, 840
BmiI GGNNCC 3 cut(s) 322, 652, 667
BmrFI CCNGG 2 cut(s) 686, 770
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 2 cut(s) 338, 875
BmuI ACTGGG 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 425
BpmI CTGGAG 1 cut(s) 385
Bpu14I TTCGAA 2 cut(s) 178, 599
BpuEI CTTGAG 1 cut(s) 817
BsaBI GATNNNNATC 1 cut(s) 865
BsaJI CCNNGG 2 cut(s) 324, 684
BsaWI WCCGGW 2 cut(s) 258, 984
Bsc4I CCNNNNNNNGG 1 cut(s) 345
Bse1I ACTGG 5 cut(s) 112, 159, 403, 436, 639
Bse8I GATNNNNATC 1 cut(s) 865
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 2 cut(s) 686, 770
BseDI CCNNGG 2 cut(s) 324, 684
BseGI GGATG 4 cut(s) 258, 329, 936, 954
BseJI GATNNNNATC 1 cut(s) 865
BseLI CCNNNNNNNGG 1 cut(s) 345
BseMII CTCAG 2 cut(s) 155, 577
BseNI ACTGG 5 cut(s) 112, 159, 403, 436, 639
BseXI GCAGC 3 cut(s) 4, 400, 931
BsgI GTGCAG 1 cut(s) 938
BshFI GGCC 3 cut(s) 606, 822, 842
BshNI GGYRCC 1 cut(s) 665
BsiSI CCGG 2 cut(s) 259, 985
BslI CCNNNNNNNGG 1 cut(s) 345
BsmAI GTCTC 2 cut(s) 458, 754
BsmBI CGTCTC 1 cut(s) 458
BsmI GAATGC 2 cut(s) 72, 93
BsnI GGCC 3 cut(s) 606, 822, 842
Bsp119I TTCGAA 2 cut(s) 178, 599
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 4 cut(s) 183, 650, 729, 995
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 2 cut(s) 369, 1016
BspANI GGCC 3 cut(s) 606, 822, 842
BspCNI CTCAG 2 cut(s) 156, 578
BspEI TCCGGA 1 cut(s) 258
BspLI GGNNCC 3 cut(s) 322, 652, 667
BspMAI CTGCAG 1 cut(s) 738
BspPI GGATC 5 cut(s) 191, 645, 658, 724, 990
BspT104I TTCGAA 2 cut(s) 178, 599
BspT107I GGYRCC 1 cut(s) 665
BsrBI CCGCTC 1 cut(s) 369
BsrI ACTGG 5 cut(s) 112, 159, 403, 436, 639
BssECI CCNNGG 2 cut(s) 324, 684
BssMI GATC 4 cut(s) 183, 650, 729, 995
BssNAI GTATAC 1 cut(s) 691
BssT1I CCWWGG 1 cut(s) 324
Bst1107I GTATAC 1 cut(s) 691
Bst2UI CCWGG 2 cut(s) 686, 770
Bst4CI ACNGT 2 cut(s) 270, 469
BstBI TTCGAA 2 cut(s) 178, 599
BstC8I GCNNGC 2 cut(s) 82, 238
BstDEI CTNAG 2 cut(s) 164, 586
BstDSI CCRYGG 1 cut(s) 324
BstF5I GGATG 4 cut(s) 258, 329, 936, 954
BstKTI GATC 4 cut(s) 186, 653, 732, 998
BstMAI GTCTC 2 cut(s) 458, 754
BstMBI GATC 4 cut(s) 183, 650, 729, 995
BstMWI GCNNNNNNNGC 1 cut(s) 733
BstNI CCWGG 2 cut(s) 686, 770
BstSCI CCNGG 2 cut(s) 684, 768
BstSFI CTRYAG 1 cut(s) 734
BstV1I GCAGC 3 cut(s) 4, 400, 931
BstV2I GAAGAC 1 cut(s) 425
BstX2I RGATCY 2 cut(s) 650, 995
BstXI CCANNNNNNTGG 1 cut(s) 949
BstYI RGATCY 2 cut(s) 650, 995
BstZ17I GTATAC 1 cut(s) 691
BsuRI GGCC 3 cut(s) 606, 822, 842
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 4 cut(s) 258, 329, 936, 954
BtsI GCAGTG 1 cut(s) 821
BtsIMutI CAGTG 5 cut(s) 102, 152, 161, 275, 821
Cac8I GCNNGC 2 cut(s) 82, 238
Cfr13I GGNCC 2 cut(s) 321, 840
Csp6I GTAC 3 cut(s) 403, 786, 878
CspCI CAANNNNNGTGG 3 cut(s) 704, 739, 1014
CviAII CATG 7 cut(s) 249, 286, 301, 325, 334, 352, 706
CviQI GTAC 3 cut(s) 403, 786, 878
DdeI CTNAG 2 cut(s) 164, 586
DpnI GATC 4 cut(s) 185, 652, 731, 997
DpnII GATC 4 cut(s) 183, 650, 729, 995
DraIII CACNNNGTG 1 cut(s) 167
Eco130I CCWWGG 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 321
EcoRI GAATTC 1 cut(s) 957
EcoRII CCWGG 2 cut(s) 684, 768
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 7 cut(s) 252, 289, 304, 328, 337, 355, 709
FatI CATG 7 cut(s) 248, 285, 300, 324, 333, 351, 705
FauI CCCGC 1 cut(s) 362
FauNDI CATATG 1 cut(s) 114
FblI GTMKAC 2 cut(s) 104, 690
Fnu4HI GCNGC 3 cut(s) 18, 389, 920
FokI GGATG 4 cut(s) 265, 316, 943, 961
Fsp4HI GCNGC 3 cut(s) 18, 389, 920
GluI GCNGC 3 cut(s) 18, 389, 920
GsuI CTGGAG 1 cut(s) 385
HaeIII GGCC 3 cut(s) 606, 822, 842
HapII CCGG 2 cut(s) 259, 985
Hin1II CATG 7 cut(s) 252, 289, 304, 328, 337, 355, 709
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 7 cut(s) 39, 541, 588, 596, 745, 936, 981
HpaII CCGG 2 cut(s) 259, 985
HphI GGTGA 7 cut(s) 211, 215, 327, 406, 789, 931, 999
Hpy166II GTNNAC 2 cut(s) 105, 691
Hpy188I TCNGA 5 cut(s) 533, 587, 830, 956, 1006
Hpy188III TCNNGA 6 cut(s) 215, 259, 364, 463, 796, 962
Hpy8I GTNNAC 2 cut(s) 105, 691
HpyAV CCTTC 6 cut(s) 56, 174, 256, 408, 504, 1003
HpyCH4III ACNGT 2 cut(s) 270, 469
HpyCH4IV ACGT 2 cut(s) 101, 405
HpyCH4V TGCA 6 cut(s) 84, 91, 236, 736, 835, 919
HpyF10VI GCNNNNNNNGC 1 cut(s) 733
HpyF3I CTNAG 2 cut(s) 164, 586
HpySE526I ACGT 2 cut(s) 101, 405
Hsp92II CATG 7 cut(s) 252, 289, 304, 328, 337, 355, 709
Kpn2I TCCGGA 1 cut(s) 258
Kzo9I GATC 4 cut(s) 183, 650, 729, 995
LmnI GCTCC 1 cut(s) 366
Lsp1109I GCAGC 3 cut(s) 4, 400, 931
LweI GCATC 2 cut(s) 338, 875
MaeII ACGT 2 cut(s) 101, 405
MaeIII GTNAC 9 cut(s) 97, 157, 199, 221, 372, 394, 750, 937, 987
MalI GATC 4 cut(s) 185, 652, 731, 997
MbiI CCGCTC 1 cut(s) 369
MboI GATC 4 cut(s) 183, 650, 729, 995
MboII GAAGA 6 cut(s) 423, 430, 792, 870, 915, 990
MflI RGATCY 2 cut(s) 650, 995
MluCI AATT 4 cut(s) 559, 824, 884, 957
MlyI GAGTC 3 cut(s) 48, 597, 945
MmeI TCCRAC 2 cut(s) 462, 476
MnlI CCTC 8 cut(s) 311, 338, 602, 613, 617, 732, 792, 812
MroI TCCGGA 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 70
MslI CAYNNNNRTG 3 cut(s) 271, 305, 947
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 2 cut(s) 259, 985
MspR9I CCNGG 2 cut(s) 686, 770
Mva1269I GAATGC 2 cut(s) 72, 93
MvaI CCWGG 2 cut(s) 686, 770
MwoI GCNNNNNNNGC 1 cut(s) 733
NcoI CCATGG 1 cut(s) 324
NdeI CATATG 1 cut(s) 114
NdeII GATC 4 cut(s) 183, 650, 729, 995
NlaIII CATG 7 cut(s) 252, 289, 304, 328, 337, 355, 709
NlaIV GGNNCC 3 cut(s) 322, 652, 667
NmuCI GTSAC 6 cut(s) 97, 199, 221, 394, 937, 987
NspV TTCGAA 2 cut(s) 178, 599
OliI CACNNNNGTG 1 cut(s) 271
PctI GAATGC 2 cut(s) 72, 93
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 4 cut(s) 541, 596, 745, 981
PfoI TCCNGGA 1 cut(s) 768
PkrI GCNGC 3 cut(s) 19, 390, 921
PleI GAGTC 3 cut(s) 47, 596, 944
PpsI GAGTC 3 cut(s) 47, 596, 944
Psp6I CCWGG 2 cut(s) 684, 768
PspGI CCWGG 2 cut(s) 684, 768
PspN4I GGNNCC 3 cut(s) 322, 652, 667
PspPI GGNCC 2 cut(s) 321, 840
PstI CTGCAG 1 cut(s) 738
PsuI RGATCY 2 cut(s) 650, 995
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 3 cut(s) 404, 787, 879
RsaNI GTAC 3 cut(s) 403, 786, 878
RseI CAYNNNNRTG 3 cut(s) 271, 305, 947
SatI GCNGC 3 cut(s) 18, 389, 920
Sau3AI GATC 4 cut(s) 183, 650, 729, 995
Sau96I GGNCC 2 cut(s) 321, 840
SchI GAGTC 3 cut(s) 48, 597, 945
ScrFI CCNGG 2 cut(s) 686, 770
SfaNI GCATC 2 cut(s) 338, 875
SfcI CTRYAG 1 cut(s) 734
SfuI TTCGAA 2 cut(s) 178, 599
SinI GGWCC 1 cut(s) 321
SmiMI CAYNNNNRTG 3 cut(s) 271, 305, 947
SmlI CTYRAG 1 cut(s) 796
SmoI CTYRAG 1 cut(s) 796
Sse9I AATT 4 cut(s) 559, 824, 884, 957
SsiI CCGC 2 cut(s) 369, 1016
SspI AATATT 1 cut(s) 6
StyD4I CCNGG 2 cut(s) 684, 768
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 270, 469
TaiI ACGT 2 cut(s) 104, 408
TaqI TCGA 6 cut(s) 145, 178, 462, 594, 599, 743
TasI AATT 4 cut(s) 559, 824, 884, 957
TfiI GAWTC 4 cut(s) 541, 596, 745, 981
TscAI CASTG 5 cut(s) 102, 159, 168, 275, 821
TseFI GTSAC 6 cut(s) 97, 199, 221, 394, 937, 987
TseI GCWGC 3 cut(s) 17, 388, 919
Tsp45I GTSAC 6 cut(s) 97, 199, 221, 394, 937, 987
TspDTI ATGAA 4 cut(s) 340, 722, 796, 871
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 5 cut(s) 102, 159, 168, 275, 821
VpaK11BI GGWCC 1 cut(s) 321
XapI RAATTY 1 cut(s) 957
XmiI GTMKAC 2 cut(s) 104, 690
XmnI GAANNNNTTC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.