Rroxscaffold_7G00179050

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001247
Physical Location & Seq
Forward (+)
18623002 .. 18627435
4434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179050.1

Sequence Viewer

Length: 1140 bp
ATGGATAAACATGTTGTTCCTGCCCAACCGTTAGCATCATTTGAGTATGAGTACGTGGATACTAATCCTGATCGTCTAAGAACTGTTGCAGCCTCTTCAGACCAGAAAAGTGCATGGATTGAACCTGCAAAGTTGAAACTGAGACACAGGATTGGGAGGGGACCCTTTGGTGATGTCTGGTTAGCAACCCATCATCAGTCAACTGAAGATTATGATGAGTATCATGAGGTGGCTGTCAAGATGTTTCAACCAATCAAAGAGGACAACATGAAGGCTGTGTTGGATAAACTTGAAGATGTTTTTTATAAGTGTCAAGGGGTAGAAGGTCTGTGTCGGCTACATGGAACTTCAATTATCAATGGAAAGATATGCATTGTAATGAAATTATACGAGGGATCCGTTGGTGACAAAATGGCTCACCTCAAAGGAGGGAAGCTCTCACTTCTTGATGTTTTGAGGTATGGAATCACATTGGCTATGGGGATTTCCGAACTGCACTCTAGAGAGATCTTGGTTCTTAATCCCAAACCTTGTAATGTTCTTTTAAATGAAAATGACCAAGCAATTCTTGGAGATTTTGGTATTCCTTATCTATTGCTTGGCATTCCATTGGTAAGCTCAGACATGACTCGGAGGATTGGAACTCCAAACTACATGGCACCTGAACAATGGCAACCAGAAGTAAGAGGTCCAATATCCTTTGAGACTGACTCATGGGGTTTTGGGTGCGTCATTGTAGAAATGTTGACTGGTGTACGGCCTTGGTGTGGAAAATCTGTTGATGAAATACATTTTTCAGTTGTCAGAAAGCAAGAAAAACCAGACATACCTAGTGGCCTTCCTCCTGCAATTGAGAATATTCTTCTTGGCTGCTTTGAGTATGACTTACGGAGTCGTCCTTCAATAATGGACATATTGAATGCTTTCAAAGGCTTGCAGAATGCAATCAGTGACGTAGACTGGACATATGTCGGGACTAGAGCAATCACAGAGAAATCGAGCAGCACTGGTTACACTGAGTGGTTTCTTTCAAAGGATCATCTGCTGGTGGGTGACATAGTTCGTTCCAGAAAGCCACCGAACTCCTGCAAGCCTGAAAACATGGAGTGGATGTTCCGGAAGGCACAGTGGTGGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

379

Amino Acids

42.87

Weight (kDa)

5.97

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 45 - 308 1.3e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 48 - 307 1.2e-26 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 306
Acc36I ACCTGC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 658
AccI GTMKAC 1 cut(s) 957
AccIII TCCGGA 1 cut(s) 1116
AclWI GGATC 3 cut(s) 390, 403, 1044
AcuI CTGAAG 2 cut(s) 81, 225
AdeI CACNNNGTG 1 cut(s) 1020
AfaI GTAC 2 cut(s) 53, 756
AfiI CCNNNNNNNGG 1 cut(s) 767
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 9 cut(s) 122, 136, 248, 293, 351, 903, 919, 928, 1032
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AleI CACNNNNGTG 1 cut(s) 1129
AloI GAACNNNNNNTCC 2 cut(s) 1097, 1129
AluBI AGCT 2 cut(s) 436, 618
AluI AGCT 2 cut(s) 436, 618
Alw26I GTCTC 2 cut(s) 136, 698
AlwI GGATC 3 cut(s) 390, 403, 1044
Aor13HI TCCGGA 1 cut(s) 1116
AoxI GGCC 2 cut(s) 758, 835
ApeKI GCWGC 3 cut(s) 89, 870, 1002
Asp700I GAANNNNTTC 1 cut(s) 923
AspS9I GGNCC 2 cut(s) 161, 689
AsuHPI GGTGA 4 cut(s) 182, 410, 416, 1064
AvaII GGWCC 2 cut(s) 161, 689
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 658
BbvI GCAGC 3 cut(s) 101, 857, 1014
BccI CCATC 1 cut(s) 198
BceAI ACGGC 1 cut(s) 773
BciVI GTATCC 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 136, 698
BfaI CTAG 3 cut(s) 501, 831, 978
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 1 cut(s) 52
BglII AGATCT 1 cut(s) 507
BisI GCNGC 3 cut(s) 90, 871, 1003
BlsI GCNGC 3 cut(s) 91, 872, 1004
Bme18I GGWCC 2 cut(s) 161, 689
BmgT120I GGNCC 2 cut(s) 161, 689
BmiI GGNNCC 4 cut(s) 162, 163, 397, 660
BmsI GCATC 1 cut(s) 44
BoxI GACNNNNGTC 1 cut(s) 968
BplI GAGNNNNNCTC 4 cut(s) 420, 452, 695, 727
BsaAI YACGTR 1 cut(s) 55
BsaBI GATNNNNATC 2 cut(s) 63, 219
BsaJI CCNNGG 1 cut(s) 761
BsaWI WCCGGW 1 cut(s) 1116
BsaXI ACNNNNNCTCC 2 cut(s) 1097, 1127
Bsc4I CCNNNNNNNGG 1 cut(s) 767
Bse1I ACTGG 3 cut(s) 754, 965, 1012
Bse8I GATNNNNATC 2 cut(s) 63, 219
BseAI TCCGGA 1 cut(s) 1116
BseDI CCNNGG 1 cut(s) 761
BseGI GGATG 1 cut(s) 1116
BseJI GATNNNNATC 2 cut(s) 63, 219
BseLI CCNNNNNNNGG 1 cut(s) 767
BseMII CTCAG 3 cut(s) 131, 633, 1008
BseNI ACTGG 3 cut(s) 754, 965, 1012
BseXI GCAGC 3 cut(s) 101, 857, 1014
BsgI GTGCAG 1 cut(s) 479
BshFI GGCC 2 cut(s) 760, 837
BshNI GGYRCC 1 cut(s) 658
BsiSI CCGG 1 cut(s) 1117
BslFI GGGAC 2 cut(s) 174, 988
BslI CCNNNNNNNGG 1 cut(s) 767
BsmAI GTCTC 2 cut(s) 136, 698
BsmFI GGGAC 2 cut(s) 174, 988
BsmI GAATGC 3 cut(s) 603, 925, 946
BsnI GGCC 2 cut(s) 760, 837
Bsp13I TCCGGA 1 cut(s) 1116
Bsp143I GATC 4 cut(s) 70, 395, 507, 1036
BspANI GGCC 2 cut(s) 760, 837
BspCNI CTCAG 3 cut(s) 132, 632, 1009
BspEI TCCGGA 1 cut(s) 1116
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 4 cut(s) 162, 163, 397, 660
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 3 cut(s) 390, 403, 1044
BspT107I GGYRCC 1 cut(s) 658
BsrI ACTGG 3 cut(s) 754, 965, 1012
BssECI CCNNGG 1 cut(s) 761
BssMI GATC 4 cut(s) 70, 395, 507, 1036
BssT1I CCWWGG 1 cut(s) 761
Bst4CI ACNGT 3 cut(s) 30, 85, 1128
Bst6I CTCTTC 1 cut(s) 100
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 2 cut(s) 935, 1091
BstDEI CTNAG 4 cut(s) 77, 140, 619, 1017
BstF5I GGATG 1 cut(s) 1116
BstKTI GATC 4 cut(s) 73, 398, 510, 1039
BstMAI GTCTC 2 cut(s) 136, 698
BstMBI GATC 4 cut(s) 70, 395, 507, 1036
BstNSI RCATGY 1 cut(s) 14
BstPAI GACNNNNGTC 1 cut(s) 968
BstV1I GCAGC 3 cut(s) 101, 857, 1014
BstX2I RGATCY 2 cut(s) 395, 507
BstYI RGATCY 2 cut(s) 395, 507
BsuI GTATCC 1 cut(s) 52
BsuRI GGCC 2 cut(s) 760, 837
BtsCI GGATG 1 cut(s) 1116
BtsIMutI CAGTG 4 cut(s) 955, 1005, 1014, 1133
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 935, 1091
CciI TCATGA 1 cut(s) 223
Cfr13I GGNCC 2 cut(s) 161, 689
CseI GACGC 1 cut(s) 718
Csp6I GTAC 2 cut(s) 52, 755
CviAII CATG 9 cut(s) 11, 114, 224, 268, 341, 625, 655, 714, 1102
CviQI GTAC 2 cut(s) 52, 755
DdeI CTNAG 4 cut(s) 77, 140, 619, 1017
DpnI GATC 4 cut(s) 72, 397, 509, 1038
DpnII GATC 4 cut(s) 70, 395, 507, 1036
DraI TTTAAA 1 cut(s) 546
DraIII CACNNNGTG 1 cut(s) 1020
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 761
Eco47I GGWCC 2 cut(s) 161, 689
Eco57I CTGAAG 2 cut(s) 81, 225
EcoO109I RGGNCCY 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 761
EcoT22I ATGCAT 1 cut(s) 374
ErhI CCWWGG 1 cut(s) 761
FaeI CATG 9 cut(s) 14, 117, 227, 271, 344, 628, 658, 717, 1105
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FaqI GGGAC 2 cut(s) 174, 988
FatI CATG 9 cut(s) 10, 113, 223, 267, 340, 624, 654, 713, 1101
FauNDI CATATG 1 cut(s) 967
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 3 cut(s) 90, 871, 1003
FokI GGATG 1 cut(s) 1123
Fsp4HI GCNGC 3 cut(s) 90, 871, 1003
FspBI CTAG 3 cut(s) 501, 831, 978
GluI GCNGC 3 cut(s) 90, 871, 1003
HaeIII GGCC 2 cut(s) 760, 837
HapII CCGG 1 cut(s) 1117
HgaI GACGC 1 cut(s) 718
Hin1II CATG 9 cut(s) 14, 117, 227, 271, 344, 628, 658, 717, 1105
HincII GTYRAC 2 cut(s) 201, 747
HindII GTYRAC 2 cut(s) 201, 747
HinfI GANTC 4 cut(s) 465, 628, 710, 892
HpaII CCGG 1 cut(s) 1117
HphI GGTGA 4 cut(s) 182, 410, 416, 1064
Hpy166II GTNNAC 4 cut(s) 201, 747, 755, 958
Hpy188I TCNGA 5 cut(s) 100, 490, 622, 633, 806
Hpy188III TCNNGA 8 cut(s) 68, 224, 238, 446, 501, 973, 1068, 1117
Hpy8I GTNNAC 4 cut(s) 201, 747, 755, 958
HpyAV CCTTC 5 cut(s) 265, 317, 848, 909, 1114
HpyCH4III ACNGT 3 cut(s) 30, 85, 1128
HpyCH4IV ACGT 2 cut(s) 54, 954
HpyCH4V TGCA 9 cut(s) 89, 113, 128, 372, 496, 848, 937, 944, 1089
HpyF3I CTNAG 4 cut(s) 77, 140, 619, 1017
HpySE526I ACGT 2 cut(s) 54, 954
Hsp92II CATG 9 cut(s) 14, 117, 227, 271, 344, 628, 658, 717, 1105
KflI GGGWCCC 1 cut(s) 161
Kpn2I TCCGGA 1 cut(s) 1116
Kzo9I GATC 4 cut(s) 70, 395, 507, 1036
Lsp1109I GCAGC 3 cut(s) 101, 857, 1014
LweI GCATC 1 cut(s) 44
MaeI CTAG 3 cut(s) 501, 831, 978
MaeII ACGT 2 cut(s) 54, 954
MaeIII GTNAC 4 cut(s) 404, 950, 1010, 1052
MalI GATC 4 cut(s) 72, 397, 509, 1038
MboI GATC 4 cut(s) 70, 395, 507, 1036
MboII GAAGA 4 cut(s) 87, 218, 305, 854
MfeI CAATTG 1 cut(s) 849
MflI RGATCY 2 cut(s) 395, 507
MluCI AATT 4 cut(s) 351, 383, 564, 849
MlyI GAGTC 3 cut(s) 622, 704, 901
MmeI TCCRAC 1 cut(s) 261
Mph1103I ATGCAT 1 cut(s) 374
MroI TCCGGA 1 cut(s) 1116
MroXI GAANNNNTTC 1 cut(s) 923
MseI TTAA 2 cut(s) 519, 545
MslI CAYNNNNRTG 2 cut(s) 377, 1129
MspI CCGG 1 cut(s) 1117
MunI CAATTG 1 cut(s) 849
Mva1269I GAATGC 3 cut(s) 603, 925, 946
NdeI CATATG 1 cut(s) 967
NdeII GATC 4 cut(s) 70, 395, 507, 1036
NlaIII CATG 9 cut(s) 14, 117, 227, 271, 344, 628, 658, 717, 1105
NlaIV GGNNCC 4 cut(s) 162, 163, 397, 660
NmuCI GTSAC 3 cut(s) 404, 950, 1052
NsiI ATGCAT 1 cut(s) 374
NspI RCATGY 1 cut(s) 14
OliI CACNNNNGTG 1 cut(s) 1129
PagI TCATGA 1 cut(s) 223
PciI ACATGT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PctI GAATGC 3 cut(s) 603, 925, 946
PdmI GAANNNNTTC 1 cut(s) 923
PfeI GAWTC 1 cut(s) 465
PkrI GCNGC 3 cut(s) 91, 872, 1004
PleI GAGTC 3 cut(s) 622, 704, 900
PpsI GAGTC 3 cut(s) 622, 704, 900
Ppu21I YACGTR 1 cut(s) 55
PpuMI RGGWCCY 1 cut(s) 161
PscI ACATGT 1 cut(s) 10
PshAI GACNNNNGTC 1 cut(s) 968
PsiI TTATAA 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 161
PspN4I GGNNCC 4 cut(s) 162, 163, 397, 660
PspPI GGNCC 2 cut(s) 161, 689
PspPPI RGGWCCY 1 cut(s) 161
PsuI RGATCY 2 cut(s) 395, 507
RsaI GTAC 2 cut(s) 53, 756
RsaNI GTAC 2 cut(s) 52, 755
RseI CAYNNNNRTG 2 cut(s) 377, 1129
SaqAI TTAA 2 cut(s) 519, 545
SatI GCNGC 3 cut(s) 90, 871, 1003
Sau3AI GATC 4 cut(s) 70, 395, 507, 1036
Sau96I GGNCC 2 cut(s) 161, 689
SchI GAGTC 3 cut(s) 622, 704, 901
SfaNI GCATC 1 cut(s) 44
SinI GGWCC 2 cut(s) 161, 689
SmiMI CAYNNNNRTG 2 cut(s) 377, 1129
Sse9I AATT 4 cut(s) 351, 383, 564, 849
SspI AATATT 1 cut(s) 859
SspMI CTAG 3 cut(s) 501, 831, 978
StyI CCWWGG 1 cut(s) 761
TaaI ACNGT 3 cut(s) 30, 85, 1128
TaiI ACGT 2 cut(s) 57, 957
TaqI TCGA 1 cut(s) 998
TasI AATT 4 cut(s) 351, 383, 564, 849
TfiI GAWTC 1 cut(s) 465
Tru1I TTAA 2 cut(s) 519, 545
Tru9I TTAA 2 cut(s) 519, 545
TscAI CASTG 4 cut(s) 955, 1012, 1021, 1133
TseFI GTSAC 3 cut(s) 404, 950, 1052
TseI GCWGC 3 cut(s) 89, 870, 1002
Tsp45I GTSAC 3 cut(s) 404, 950, 1052
TspDTI ATGAA 4 cut(s) 284, 395, 564, 798
TspGWI ACGGA 2 cut(s) 388, 904
TspRI CASTG 4 cut(s) 955, 1012, 1021, 1133
VpaK11BI GGWCC 2 cut(s) 161, 689
XbaI TCTAGA 1 cut(s) 500
XceI RCATGY 1 cut(s) 14
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 957
XmnI GAANNNNTTC 1 cut(s) 923
XspI CTAG 3 cut(s) 501, 831, 978
Zsp2I ATGCAT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.