RchiOBHm_Chr4g0394841

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001247
Physical Location & Seq
Reverse (-)
10597738 .. 10601543
3806 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36731

Sequence Viewer

Length: 1350 bp
ATGGAGCAGCAAATTGCAAAAGAAAAGCCTGCAATTTCTTTCGAGTATGAGCTCTTTCAAGACCCTGATCATCTAACAACTGTTGTAGCTACACTCACTCCAACTAGTCCCCGGATCAATCCTGCCTCATTGAACTTTAAGTACCGAATTGGACAGGGCCCCTTTGGTGATGTATGGTTAGCAACTCATCACCAGTCAGCAGATGATTATGATGAATATCATGAGGTAGCAGTTAAGATGTTGCATCCTTTGATGGAGGAGGACAGAGAAAAGTTTCTGCACAAGTTCGAAAAGTTATTTTTCAAGTTCCGCAGACTGTGCGGTGTTTGTTGGTTTCATGGTATCTCAATCATTGATGGAAGGATCTGCATCGCTATGAAATTTTATGAGGGGTCAGTTGGTGACCGAGTTGCTAGGTTGAAGGGTGGAAAACTTCAACTTTTTGATGTTTTAAGGTATGGGATTGAGTTGGCAAAAGGAATTTCAGAATTGCATTCACTAGGGCTTCTGGTGCTAAATTTGAAGCCGTCCAACATCCTTGTAGATGAACACGGCCAGGTGGTCCTTGGAGATTTTGGAATCCCATTTCTACTTCTTGGGGCTTCATTGTCTAATCCTAATATGGCTTTTAGACTTGGCACTCCGAACTACATGGCTCCAGAACAGTGGGAACCAGAAGTGAGGGGTCCTATATCTTTAGAAACAGATTCTTGGGGCTTTGGATGTTGCTTTGTAGAGATGTTGACTGGTAGTCAACCCTGGTTTGGGAGTTCAATTAAAGAGATATATCATTCAGTTGTGATCAAGCGAGAAAAGCCACCTGTTCCAAGTGGTTTGCCTCCTGCAGTGGAGAATGTTATCAACGGCTGCTTTGAGTATGATCTCCATAATAGGCCCTCAATGGAAGATATCATACGTGCATTGGAAAGGTATACCCACTACCATTACCCACTACCATTACCCAATAATGGTAGAGGTTTCAAGGTCCGTGAGAGTATCACTAGGTCCTTAGCTATAGAGGAACCTGGAGACCATGGAGAAGCACACTTAGTAGCTCTGGGTGAACTAGAATTACAGATTCAAGATGAGGAGACAAATGGTTACTCGGCACAATATTCGTCAACTGGTTTTACTGATATGGGGAACACAGCAATATTATCTACCTTCAGCACGAGAACTATCAAGATTGGAGTACGCAAAGCTCCTGTGATACAACATTGTGCCGAAACAATTTCAGGATCAACTACTATTAGGAGCTTTGACCAGCATACTGGTTGTTTATATATGTGCTTCCTCATATATATGCTCAACTCCTTATCAGTTTTAGCCGGCTTTACTCCTTCGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1 Pkinase Pkinase_Tyr

Protein Analysis

449

Amino Acids

50.37

Weight (kDa)

5.72

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 45 - 308 7.5e-32 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 47 - 305 4e-28 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 932
AciI CCGC 2 cut(s) 310, 321
AclWI GGATC 3 cut(s) 122, 371, 1246
AcoI YGGCCR 1 cut(s) 553
AcsI RAATTY 3 cut(s) 380, 480, 517
AcuI CTGAAG 1 cut(s) 1150
AfaI GTAC 2 cut(s) 143, 1194
AfiI CCNNNNNNNGG 3 cut(s) 764, 765, 968
AgsI TTSAA 9 cut(s) 59, 133, 304, 421, 437, 523, 774, 982, 1082
AhdI GACNNNNNGTC 1 cut(s) 750
AhlI ACTAGT 1 cut(s) 104
AjnI CCWGG 3 cut(s) 555, 758, 1024
AluBI AGCT 6 cut(s) 52, 89, 1013, 1055, 1202, 1257
AluI AGCT 6 cut(s) 52, 89, 1013, 1055, 1202, 1257
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 2 cut(s) 1023, 1085
AlwI GGATC 3 cut(s) 122, 371, 1246
AoxI GGCC 3 cut(s) 157, 553, 893
ApaI GGGCCC 1 cut(s) 161
ApeKI GCWGC 2 cut(s) 7, 867
ApoI RAATTY 3 cut(s) 380, 480, 517
Asp700I GAANNNNTTC 1 cut(s) 273
AspS9I GGNCC 7 cut(s) 157, 158, 562, 686, 894, 985, 1005
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 4 cut(s) 179, 182, 413, 1073
AsuII TTCGAA 1 cut(s) 288
AvaII GGWCC 4 cut(s) 562, 686, 985, 1005
BaeGI GKGCMC 1 cut(s) 161
BanII GRGCYC 2 cut(s) 54, 161
BarI GAAGNNNNNNTAC 2 cut(s) 897, 929
BauI CACGAG 1 cut(s) 1171
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 19, 854
BccI CCATC 2 cut(s) 247, 350
BceAI ACGGC 3 cut(s) 511, 568, 880
BcgI CGANNNNNNTGC 2 cut(s) 1098, 1132
BciT130I CCWGG 3 cut(s) 557, 760, 1026
BclI TGATCA 2 cut(s) 67, 801
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 2 cut(s) 1023, 1085
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 5 cut(s) 105, 414, 500, 1002, 1067
BfmI CTRYAG 2 cut(s) 843, 1014
BisI GCNGC 2 cut(s) 8, 868
BlsI GCNGC 2 cut(s) 9, 869
Bme1390I CCNGG 4 cut(s) 112, 557, 760, 1026
Bme18I GGWCC 4 cut(s) 562, 686, 985, 1005
BmeRI GACNNNNNGTC 1 cut(s) 750
BmgT120I GGNCC 7 cut(s) 157, 158, 562, 686, 894, 985, 1005
BmiI GGNNCC 6 cut(s) 159, 160, 657, 672, 687, 1023
BmrFI CCNGG 4 cut(s) 112, 557, 760, 1026
BmsI GCATC 2 cut(s) 253, 378
BpmI CTGGAG 2 cut(s) 642, 1047
Bpu10I CCTNAGC 1 cut(s) 1009
Bpu14I TTCGAA 1 cut(s) 288
BpuMI CCSGG 1 cut(s) 112
BsaAI YACGTR 1 cut(s) 917
BsaBI GATNNNNATC 2 cut(s) 216, 368
BsaI GGTCTC 1 cut(s) 1023
BsaJI CCNNGG 4 cut(s) 110, 565, 758, 1033
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 3 cut(s) 764, 765, 968
Bse118I RCCGGY 1 cut(s) 1328
Bse1I ACTGG 4 cut(s) 193, 751, 1129, 1276
Bse8I GATNNNNATC 2 cut(s) 216, 368
BseBI CCWGG 3 cut(s) 557, 760, 1026
BseDI CCNNGG 4 cut(s) 110, 565, 758, 1033
BseGI GGATG 3 cut(s) 244, 534, 728
BseJI GATNNNNATC 2 cut(s) 216, 368
BseLI CCNNNNNNNGG 3 cut(s) 764, 765, 968
BseNI ACTGG 4 cut(s) 193, 751, 1129, 1276
BseRI GAGGAG 2 cut(s) 272, 1103
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 2 cut(s) 19, 854
BsgI GTGCAG 1 cut(s) 263
BshFI GGCC 3 cut(s) 159, 555, 895
BsiHKAI GWGCWC 1 cut(s) 54
BsiSI CCGG 2 cut(s) 112, 1329
BslFI GGGAC 1 cut(s) 93
BslI CCNNNNNNNGG 3 cut(s) 764, 765, 968
BsmAI GTCTC 2 cut(s) 1023, 1085
BsmFI GGGAC 1 cut(s) 93
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 3 cut(s) 159, 555, 895
Bso31I GGTCTC 1 cut(s) 1023
Bsp119I TTCGAA 1 cut(s) 288
Bsp120I GGGCCC 1 cut(s) 157
Bsp1286I GDGCHC 2 cut(s) 54, 161
Bsp143I GATC 6 cut(s) 67, 114, 363, 801, 880, 1238
Bsp19I CCATGG 1 cut(s) 1033
BspACI CCGC 2 cut(s) 310, 321
BspANI GGCC 3 cut(s) 159, 555, 895
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 6 cut(s) 159, 160, 657, 672, 687, 1023
BspMAI CTGCAG 1 cut(s) 847
BspPI GGATC 3 cut(s) 122, 371, 1246
BspT104I TTCGAA 1 cut(s) 288
BspTNI GGTCTC 1 cut(s) 1023
BsrFI RCCGGY 1 cut(s) 1328
BsrI ACTGG 4 cut(s) 193, 751, 1129, 1276
BssAI RCCGGY 1 cut(s) 1328
BssECI CCNNGG 4 cut(s) 110, 565, 758, 1033
BssMI GATC 6 cut(s) 67, 114, 363, 801, 880, 1238
BssNAI GTATAC 1 cut(s) 933
BssSI CACGAG 1 cut(s) 1171
BssT1I CCWWGG 2 cut(s) 565, 1033
Bst1107I GTATAC 1 cut(s) 933
Bst2BI CACGAG 1 cut(s) 1171
Bst2UI CCWGG 3 cut(s) 557, 760, 1026
Bst4CI ACNGT 3 cut(s) 82, 318, 666
BstAPI GCANNNNNTGC 1 cut(s) 318
BstBAI YACGTR 1 cut(s) 917
BstBI TTCGAA 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 30, 1330
BstDEI CTNAG 2 cut(s) 1009, 1048
BstDSI CCRYGG 1 cut(s) 1033
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 3 cut(s) 244, 534, 728
BstKTI GATC 6 cut(s) 70, 117, 366, 804, 883, 1241
BstMAI GTCTC 2 cut(s) 1023, 1085
BstMBI GATC 6 cut(s) 67, 114, 363, 801, 880, 1238
BstMWI GCNNNNNNNGC 3 cut(s) 318, 511, 814
BstNI CCWGG 3 cut(s) 557, 760, 1026
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 4 cut(s) 110, 555, 758, 1024
BstSFI CTRYAG 2 cut(s) 843, 1014
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 2 cut(s) 19, 854
BstX2I RGATCY 1 cut(s) 363
BstXI CCANNNNNNTGG 2 cut(s) 666, 1271
BstYI RGATCY 1 cut(s) 363
BstZ17I GTATAC 1 cut(s) 933
BsuRI GGCC 3 cut(s) 159, 555, 895
BtgI CCRYGG 1 cut(s) 1033
BtgZI GCGATG 1 cut(s) 355
BtsCI GGATG 3 cut(s) 244, 534, 728
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 2 cut(s) 671, 852
Cac8I GCNNGC 2 cut(s) 30, 1330
CciI TCATGA 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 1328
Cfr13I GGNCC 7 cut(s) 157, 158, 562, 686, 894, 985, 1005
Csp6I GTAC 2 cut(s) 142, 1193
CviAII CATG 4 cut(s) 221, 338, 652, 1034
CviQI GTAC 2 cut(s) 142, 1193
DdeI CTNAG 2 cut(s) 1009, 1048
DpnI GATC 6 cut(s) 69, 116, 365, 803, 882, 1240
DpnII GATC 6 cut(s) 67, 114, 363, 801, 880, 1238
DriI GACNNNNNGTC 1 cut(s) 750
EaeI YGGCCR 1 cut(s) 553
Eam1105I GACNNNNNGTC 1 cut(s) 750
Ecl136II GAGCTC 1 cut(s) 52
Eco130I CCWWGG 2 cut(s) 565, 1033
Eco24I GRGCYC 2 cut(s) 54, 161
Eco31I GGTCTC 1 cut(s) 1023
Eco32I GATATC 1 cut(s) 910
Eco47I GGWCC 4 cut(s) 562, 686, 985, 1005
Eco53kI GAGCTC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 1150
Eco91I GGTNACC 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 52
EcoO109I RGGNCCY 5 cut(s) 157, 158, 686, 894, 1005
EcoO65I GGTNACC 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 555, 758, 1024
EcoRV GATATC 1 cut(s) 910
EcoT14I CCWWGG 2 cut(s) 565, 1033
EcoT38I GRGCYC 2 cut(s) 54, 161
ErhI CCWWGG 2 cut(s) 565, 1033
FaeI CATG 4 cut(s) 224, 341, 655, 1037
FaqI GGGAC 1 cut(s) 93
FatI CATG 4 cut(s) 220, 337, 651, 1033
FbaI TGATCA 2 cut(s) 67, 801
FblI GTMKAC 1 cut(s) 932
Fnu4HI GCNGC 2 cut(s) 8, 868
FokI GGATG 3 cut(s) 231, 521, 735
FriOI GRGCYC 2 cut(s) 54, 161
Fsp4HI GCNGC 2 cut(s) 8, 868
FspBI CTAG 5 cut(s) 105, 414, 500, 1002, 1067
GluI GCNGC 2 cut(s) 8, 868
GsuI CTGGAG 2 cut(s) 642, 1047
HaeIII GGCC 3 cut(s) 159, 555, 895
HapII CCGG 2 cut(s) 112, 1329
Hin1II CATG 4 cut(s) 224, 341, 655, 1037
HincII GTYRAC 3 cut(s) 744, 755, 1122
HindII GTYRAC 3 cut(s) 744, 755, 1122
HinfI GANTC 3 cut(s) 579, 707, 1078
HpaII CCGG 2 cut(s) 112, 1329
HphI GGTGA 4 cut(s) 179, 182, 413, 1073
Hpy166II GTNNAC 5 cut(s) 744, 755, 933, 1064, 1122
Hpy188I TCNGA 2 cut(s) 487, 645
Hpy188III TCNNGA 6 cut(s) 59, 221, 659, 1082, 1183, 1236
Hpy8I GTNNAC 5 cut(s) 744, 755, 933, 1064, 1122
HpyAV CCTTC 4 cut(s) 354, 415, 1174, 1350
HpyCH4III ACNGT 3 cut(s) 82, 318, 666
HpyCH4IV ACGT 1 cut(s) 916
HpyCH4V TGCA 8 cut(s) 17, 32, 244, 280, 369, 493, 845, 920
HpyF10VI GCNNNNNNNGC 3 cut(s) 318, 511, 814
HpyF3I CTNAG 2 cut(s) 1009, 1048
HpySE526I ACGT 1 cut(s) 916
Hsp92II CATG 4 cut(s) 224, 341, 655, 1037
KroI GCCGGC 1 cut(s) 1328
KroNI GCCGGC 1 cut(s) 1330
Ksp22I TGATCA 2 cut(s) 67, 801
Kzo9I GATC 6 cut(s) 67, 114, 363, 801, 880, 1238
LmnI GCTCC 4 cut(s) 4, 661, 1207, 1254
Lsp1109I GCAGC 2 cut(s) 19, 854
LweI GCATC 2 cut(s) 253, 378
MaeI CTAG 5 cut(s) 105, 414, 500, 1002, 1067
MaeII ACGT 1 cut(s) 916
MaeIII GTNAC 2 cut(s) 401, 1100
MalI GATC 6 cut(s) 69, 116, 365, 803, 882, 1240
MboI GATC 6 cut(s) 67, 114, 363, 801, 880, 1238
MboII GAAGA 1 cut(s) 917
MflI RGATCY 1 cut(s) 363
MhlI GDGCHC 2 cut(s) 54, 161
MmeI TCCRAC 2 cut(s) 125, 555
MroNI GCCGGC 1 cut(s) 1328
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 4 cut(s) 138, 234, 452, 777
MslI CAYNNNNRTG 2 cut(s) 374, 1301
MspI CCGG 2 cut(s) 112, 1329
MspR9I CCNGG 4 cut(s) 112, 557, 760, 1026
Mva1269I GAATGC 1 cut(s) 493
MvaI CCWGG 3 cut(s) 557, 760, 1026
MwoI GCNNNNNNNGC 3 cut(s) 318, 511, 814
NaeI GCCGGC 1 cut(s) 1330
NciI CCSGG 1 cut(s) 112
NcoI CCATGG 1 cut(s) 1033
NdeII GATC 6 cut(s) 67, 114, 363, 801, 880, 1238
NgoMIV GCCGGC 1 cut(s) 1328
NlaIII CATG 4 cut(s) 224, 341, 655, 1037
NlaIV GGNNCC 6 cut(s) 159, 160, 657, 672, 687, 1023
NmeAIII GCCGAG 1 cut(s) 1085
NmuCI GTSAC 1 cut(s) 401
NspV TTCGAA 1 cut(s) 288
PagI TCATGA 1 cut(s) 220
PctI GAATGC 1 cut(s) 493
PdiI GCCGGC 1 cut(s) 1330
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 3 cut(s) 579, 707, 1078
PkrI GCNGC 2 cut(s) 9, 869
Ppu21I YACGTR 1 cut(s) 917
PpuMI RGGWCCY 2 cut(s) 686, 1005
Psp124BI GAGCTC 1 cut(s) 54
Psp5II RGGWCCY 2 cut(s) 686, 1005
Psp6I CCWGG 3 cut(s) 555, 758, 1024
PspEI GGTNACC 1 cut(s) 401
PspGI CCWGG 3 cut(s) 555, 758, 1024
PspN4I GGNNCC 6 cut(s) 159, 160, 657, 672, 687, 1023
PspOMI GGGCCC 1 cut(s) 157
PspPI GGNCC 7 cut(s) 157, 158, 562, 686, 894, 985, 1005
PspPPI RGGWCCY 2 cut(s) 686, 1005
PsrI GAACNNNNNNTAC 4 cut(s) 125, 157, 1056, 1088
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 363
RsaI GTAC 2 cut(s) 143, 1194
RsaNI GTAC 2 cut(s) 142, 1193
RseI CAYNNNNRTG 2 cut(s) 374, 1301
SacI GAGCTC 1 cut(s) 54
SaqAI TTAA 4 cut(s) 138, 234, 452, 777
SatI GCNGC 2 cut(s) 8, 868
Sau3AI GATC 6 cut(s) 67, 114, 363, 801, 880, 1238
Sau96I GGNCC 7 cut(s) 157, 158, 562, 686, 894, 985, 1005
ScrFI CCNGG 4 cut(s) 112, 557, 760, 1026
SduI GDGCHC 2 cut(s) 54, 161
SfaNI GCATC 2 cut(s) 253, 378
SfcI CTRYAG 2 cut(s) 843, 1014
SfuI TTCGAA 1 cut(s) 288
SinI GGWCC 4 cut(s) 562, 686, 985, 1005
SmiMI CAYNNNNRTG 2 cut(s) 374, 1301
SpeI ACTAGT 1 cut(s) 104
SsiI CCGC 2 cut(s) 310, 321
SspI AATATT 2 cut(s) 1115, 1155
SspMI CTAG 5 cut(s) 105, 414, 500, 1002, 1067
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 4 cut(s) 110, 555, 758, 1024
StyI CCWWGG 2 cut(s) 565, 1033
TaaI ACNGT 3 cut(s) 82, 318, 666
TaiI ACGT 1 cut(s) 919
TaqI TCGA 2 cut(s) 42, 288
TaqII GACCGA 1 cut(s) 420
TfiI GAWTC 3 cut(s) 579, 707, 1078
Tru1I TTAA 4 cut(s) 138, 234, 452, 777
Tru9I TTAA 4 cut(s) 138, 234, 452, 777
TscAI CASTG 2 cut(s) 671, 852
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 2 cut(s) 7, 867
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 5 cut(s) 228, 326, 392, 561, 594
TspGWI ACGGA 1 cut(s) 977
TspRI CASTG 2 cut(s) 671, 852
VpaK11BI GGWCC 4 cut(s) 562, 686, 985, 1005
XapI RAATTY 3 cut(s) 380, 480, 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 932
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 5 cut(s) 105, 414, 500, 1002, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.