MD16G1269500.v1.1
MYB Family

At2g29880-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006437
Physical Location & Seq
Reverse (-)
34798355 .. 34798911
557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1269500.v1.1.491

Sequence Viewer

Length: 459 bp
ATGAAAACATTGAAGGGTCGATTTCAATCAATTTGCCAGCTTTTTGGACATAGTTCTGGATTTGGTTGGGACCCTATCACAAAGAAGTTCACAGCTAGTGATGAAGTATGGGAAGGCTTCCTTAGGTCTCACCCAAAGAGTCAAGGTATTCGCAAAGAGATATTTGCAGACTATGAAGATTTGATGACTGTTTTTGGTGATGGAACAGCTAAAGGAAATAACTTGATTGGATTGGGCGATGATACTGATGCCACAACATATAGAGTTGAAGAAAGTAGGCCAATCAGAGTAGACGAATTTCCTCCCTTTGATGAGAACGTCGAGCAACTTGATCCATTATTCCAAACGCCATCATATGCTTCATCTTTCCTAGATGCAAATTTTGAGGCTCCCGCTGCTACAACCATAGAAAAGCTACTTCCAAGGAAAAGATCTCAACCTAAGTCCGAACCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-bind_3

Protein Analysis

153

Amino Acids

17.11

Weight (kDa)

4.96

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 1 - 38 4.7e-07 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 291
AciI CCGC 1 cut(s) 393
AclWI GGATC 1 cut(s) 326
AcsI RAATTY 2 cut(s) 296, 379
AgsI TTSAA 3 cut(s) 13, 26, 269
AluBI AGCT 4 cut(s) 40, 95, 209, 415
AluI AGCT 4 cut(s) 40, 95, 209, 415
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 1 cut(s) 326
AoxI GGCC 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 2 cut(s) 296, 379
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 122, 209
AvaII GGWCC 1 cut(s) 70
AxyI CCTNAGG 1 cut(s) 122
BbvI GCAGC 1 cut(s) 382
BccI CCATC 2 cut(s) 194, 358
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 2 cut(s) 96, 371
BglII AGATCT 1 cut(s) 431
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 3 cut(s) 71, 72, 390
BmsI GCATC 2 cut(s) 238, 364
BsaBI GATNNNNATC 1 cut(s) 25
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 422
Bse21I CCTNAGG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 25
BseDI CCNNGG 1 cut(s) 422
BseJI GATNNNNATC 1 cut(s) 25
BseXI GCAGC 1 cut(s) 382
BshFI GGCC 1 cut(s) 280
BslFI GGGAC 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 1 cut(s) 280
Bso31I GGTCTC 1 cut(s) 132
Bsp143I GATC 2 cut(s) 331, 431
BspACI CCGC 1 cut(s) 393
BspANI GGCC 1 cut(s) 280
BspLI GGNNCC 3 cut(s) 71, 72, 390
BspPI GGATC 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 132
BssECI CCNNGG 1 cut(s) 422
BssMI GATC 2 cut(s) 331, 431
BssT1I CCWWGG 1 cut(s) 422
Bst4CI ACNGT 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 2 cut(s) 122, 441
BstKTI GATC 2 cut(s) 334, 434
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 331, 431
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstV1I GCAGC 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 431
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 1 cut(s) 431
Bsu36I CCTNAGG 1 cut(s) 122
BsuRI GGCC 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 70
CviJI RGCY 7 cut(s) 40, 95, 117, 209, 280, 389, 415
CviKI_1 RGCY 7 cut(s) 40, 95, 117, 209, 280, 389, 415
DdeI CTNAG 2 cut(s) 122, 441
DpnI GATC 2 cut(s) 333, 433
DpnII GATC 2 cut(s) 331, 431
Eco130I CCWWGG 1 cut(s) 422
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 70
Eco81I CCTNAGG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 422
FaiI YATR 8 cut(s) 51, 109, 174, 259, 261, 355, 357, 407
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FaqI GGGAC 1 cut(s) 83
FauI CCCGC 1 cut(s) 400
FauNDI CATATG 1 cut(s) 355
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 2 cut(s) 96, 371
GluI GCNGC 1 cut(s) 396
HaeIII GGCC 1 cut(s) 280
HinfI GANTC 1 cut(s) 139
HphI GGTGA 2 cut(s) 122, 209
Hpy166II GTNNAC 2 cut(s) 90, 292
Hpy188I TCNGA 2 cut(s) 287, 448
Hpy188III TCNNGA 1 cut(s) 57
Hpy8I GTNNAC 2 cut(s) 90, 292
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 2 cut(s) 7, 107
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 2 cut(s) 167, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 2 cut(s) 122, 441
HpySE526I ACGT 1 cut(s) 318
KflI GGGWCCC 1 cut(s) 70
Kzo9I GATC 2 cut(s) 331, 431
LmnI GCTCC 1 cut(s) 394
LpnPI CCDG 2 cut(s) 42, 50
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 2 cut(s) 238, 364
MaeI CTAG 2 cut(s) 96, 371
MaeII ACGT 1 cut(s) 318
MalI GATC 2 cut(s) 333, 433
MboI GATC 2 cut(s) 331, 431
MboII GAAGA 2 cut(s) 188, 281
MflI RGATCY 1 cut(s) 431
MluCI AATT 4 cut(s) 30, 296, 379, 454
MlyI GAGTC 1 cut(s) 148
MnlI CCTC 2 cut(s) 312, 379
MseI TTAA 1 cut(s) 457
MspA1I CMGCKG 1 cut(s) 395
MwoI GCNNNNNNNGC 1 cut(s) 395
NdeI CATATG 1 cut(s) 355
NdeII GATC 2 cut(s) 331, 431
NlaIV GGNNCC 3 cut(s) 71, 72, 390
PkrI GCNGC 1 cut(s) 397
PleI GAGTC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 147
PpuMI RGGWCCY 1 cut(s) 70
Psp5II RGGWCCY 1 cut(s) 70
PspN4I GGNNCC 3 cut(s) 71, 72, 390
PspPI GGNCC 1 cut(s) 70
PspPPI RGGWCCY 1 cut(s) 70
PsuI RGATCY 1 cut(s) 431
SaqAI TTAA 1 cut(s) 457
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 2 cut(s) 331, 431
Sau96I GGNCC 1 cut(s) 70
SchI GAGTC 1 cut(s) 148
SetI ASST 8 cut(s) 42, 97, 128, 148, 211, 321, 417, 442
SfaNI GCATC 2 cut(s) 238, 364
SinI GGWCC 1 cut(s) 70
Sse9I AATT 4 cut(s) 30, 296, 379, 454
SsiI CCGC 1 cut(s) 393
SspMI CTAG 2 cut(s) 96, 371
StyI CCWWGG 1 cut(s) 422
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 1 cut(s) 321
TaqI TCGA 2 cut(s) 19, 321
TasI AATT 4 cut(s) 30, 296, 379, 454
Tru1I TTAA 1 cut(s) 457
Tru9I TTAA 1 cut(s) 457
TseI GCWGC 1 cut(s) 395
TspDTI ATGAA 4 cut(s) 17, 117, 189, 351
VpaK11BI GGWCC 1 cut(s) 70
XapI RAATTY 2 cut(s) 296, 379
XmiI GTMKAC 1 cut(s) 291
XspI CTAG 2 cut(s) 96, 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.