pycom01g17160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005293
Physical Location & Seq
Forward (+)
16552353 .. 16553626
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1110 bp
ATGCACGGCGGCGCCACCTCAGCACCACCCCCAACACCCTCCAACAACCTCATGTCCACCTCAGATGCCGCGACAGCAGATGCCCTCTCGAGGCTCCTCCACCGTCTTCCTCCCACTCTCTCGTCCCATCCCACACGCCGCTCACCTCCCACGATGTGTCCACAATCGATCTCTTTCTCCGACGTCCAGGCCGATCCTACCCTCCTCTCCTGTGCTTCTCAACTTGGGTTCTTCCAGCTCACCAACCACCCCATCCCATCCCAACTCGCCAACTCGGCCGAGTCAGAAGCGCTCTCTCTGTTCGACCTTCCCGCACACCAAAAACAATCATCCGTCCCCAAGAGCTGGCCCTTAGGGTTTGAAGACGACGACGAGGATAACGGAGACGGACTCGGTGAGTCGTTCTGTTTGGACTCGTTGTGTTCCACCGAGTCGACCGAGTTGACCTTAACTTCTTTGCGCGAGTTCACTCGTGCTTTGGAGAAAGTGGGGATGGAGGTCATCGAGCTGCTTTGTAGCTCATTAGGGTTTCAGAACCCACTAGGGAAAGATGACCCGACCCGGTTTTCTACCATGATGTGGATCTCTGAGGGCTTACCCGATAAGAAGTCGCAGACGGCGGGTCGGTTTTACCCGTATGTAGTCGGGTTACAGTACCAAATCCGAAGTAGTCAGAAGTACTCTTTGCTGGCGGATTCCGGTTGGCTCTCGGTGTTACCGCAGGTGGATTCAATCATGGTTACCATTGGTGACATTGCTCAAGTATGGAGTAATGGGAAGTTGAAGAAAGTGAGAGGAAGGCCAGTGGCATGTTTGGGAAACGACTCCAAGCCATCAGGTCGCTGCATATCAATGTCGCTGTTGGTGACTCTTCCCATGGACAGTACATTGTCTCCTCTTCTTCAAATTGCAGCCATTGATGGAAATGACAGAGATGATGATAATGATGATGAAGGAGATCAAATCGACGCTAGATGTGGGGGTGGCGAAAGAGAAGCAGGAAGGTTGTTTAAGTCATTTTGTCTCGAAGACTATGCTTGGAGAGTGTATCATGAACGCCTATTTTTCAAAGATCCACTGGACAGATATCGTATCAAGAAACAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

370

Amino Acids

40.57

Weight (kDa)

4.95

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 70 - 120 2.2e-07 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 712
AatII GACGTC 1 cut(s) 186
Acc36I ACCTGC 1 cut(s) 712
AccB1I GGYRCC 1 cut(s) 11
AccB7I CCANNNNNTGG 2 cut(s) 345, 579
AccBSI CCGCTC 1 cut(s) 141
AccI GTMKAC 1 cut(s) 434
AccII CGCG 2 cut(s) 71, 462
AciI CCGC 7 cut(s) 9, 69, 139, 312, 620, 692, 719
AclWI GGATC 3 cut(s) 188, 590, 1067
AcoI YGGCCR 1 cut(s) 276
AcyI GRCGYC 2 cut(s) 12, 183
AdeI CACNNNGTG 1 cut(s) 156
AfaI GTAC 3 cut(s) 656, 680, 886
AfeI AGCGCT 1 cut(s) 291
AfiI CCNNNNNNNGG 3 cut(s) 90, 345, 579
AgsI TTSAA 5 cut(s) 362, 732, 784, 905, 1069
AhdI GACNNNNNGTC 1 cut(s) 621
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 4 cut(s) 238, 345, 508, 519
AluI AGCT 4 cut(s) 238, 345, 508, 519
Alw26I GTCTC 3 cut(s) 378, 897, 1028
AlwI GGATC 3 cut(s) 188, 590, 1067
Ama87I CYCGRG 1 cut(s) 88
Aor51HI AGCGCT 1 cut(s) 291
AoxI GGCC 4 cut(s) 189, 276, 347, 800
ApeKI GCWGC 3 cut(s) 508, 843, 911
AspLEI GCGC 3 cut(s) 14, 292, 462
AspS9I GGNCC 1 cut(s) 348
AsuC2I CCSGG 1 cut(s) 562
AsuHPI GGTGA 5 cut(s) 135, 232, 407, 761, 877
AvaI CYCGRG 1 cut(s) 88
AxyI CCTNAGG 1 cut(s) 352
BanI GGYRCC 1 cut(s) 11
BauI CACGAG 1 cut(s) 471
BbsI GAAGAC 3 cut(s) 98, 369, 1035
BbvCI CCTCAGC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 495, 830, 923
BccI CCATC 6 cut(s) 135, 260, 265, 487, 841, 914
BceAI ACGGC 2 cut(s) 22, 633
BcgI CGANNNNNNTGC 2 cut(s) 1016, 1050
BciT130I CCWGG 1 cut(s) 188
BcnI CCSGG 1 cut(s) 562
BcoDI GTCTC 3 cut(s) 378, 897, 1028
BfaI CTAG 2 cut(s) 542, 972
BfoI RGCGCY 2 cut(s) 15, 293
BfuAI ACCTGC 1 cut(s) 712
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 6 cut(s) 10, 69, 139, 509, 844, 912
BlsI GCNGC 6 cut(s) 11, 70, 140, 510, 845, 913
BmcAI AGTACT 1 cut(s) 680
Bme1390I CCNGG 2 cut(s) 188, 562
BmeRI GACNNNNNGTC 1 cut(s) 621
BmeT110I CYCGRG 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 2 cut(s) 13, 95
BmrFI CCNGG 2 cut(s) 188, 562
BmsI GCATC 2 cut(s) 55, 70
BpiI GAAGAC 3 cut(s) 98, 369, 1035
BplI GAGNNNNNCTC 2 cut(s) 375, 407
Bpu10I CCTNAGC 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 744
BpuMI CCSGG 1 cut(s) 562
Bsa29I ATCGAT 1 cut(s) 167
BsaBI GATNNNNATC 1 cut(s) 581
BsaHI GRCGYC 2 cut(s) 12, 183
BsaJI CCNNGG 1 cut(s) 876
BsaWI WCCGGW 1 cut(s) 698
BsaXI ACNNNNNCTCC 2 cut(s) 877, 907
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 345, 579
Bse1I ACTGG 2 cut(s) 803, 1083
Bse21I CCTNAGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 753
Bse8I GATNNNNATC 1 cut(s) 581
BseBI CCWGG 1 cut(s) 188
BseCI ATCGAT 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 876
BseGI GGATG 5 cut(s) 127, 252, 257, 329, 498
BseJI GATNNNNATC 1 cut(s) 581
BseLI CCNNNNNNNGG 3 cut(s) 90, 345, 579
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 3 cut(s) 33, 75, 579
BseNI ACTGG 2 cut(s) 803, 1083
BseRI GAGGAG 3 cut(s) 86, 194, 885
BseX3I CGGCCG 1 cut(s) 276
BseXI GCAGC 3 cut(s) 495, 830, 923
Bsh1236I CGCG 2 cut(s) 71, 462
Bsh1285I CGRYCG 2 cut(s) 279, 438
BshFI GGCC 4 cut(s) 191, 278, 349, 802
BshNI GGYRCC 1 cut(s) 11
BshVI ATCGAT 1 cut(s) 167
BsiEI CGRYCG 2 cut(s) 279, 438
BsiHKCI CYCGRG 1 cut(s) 88
BsiSI CCGG 2 cut(s) 562, 699
BslFI GGGAC 2 cut(s) 109, 320
BslI CCNNNNNNNGG 3 cut(s) 90, 345, 579
BsmAI GTCTC 3 cut(s) 378, 897, 1028
BsmBI CGTCTC 1 cut(s) 378
BsmFI GGGAC 2 cut(s) 109, 320
BsnI GGCC 4 cut(s) 191, 278, 349, 802
BsoBI CYCGRG 1 cut(s) 88
Bsp143I GATC 5 cut(s) 168, 193, 582, 958, 1072
Bsp19I CCATGG 1 cut(s) 876
BspACI CCGC 7 cut(s) 9, 69, 139, 312, 620, 692, 719
BspANI GGCC 4 cut(s) 191, 278, 349, 802
BspCNI CTCAG 3 cut(s) 32, 74, 580
BspDI ATCGAT 1 cut(s) 167
BspFNI CGCG 2 cut(s) 71, 462
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 2 cut(s) 13, 95
BspMI ACCTGC 1 cut(s) 712
BspPI GGATC 3 cut(s) 188, 590, 1067
BspT107I GGYRCC 1 cut(s) 11
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 753
BsrI ACTGG 2 cut(s) 803, 1083
BssECI CCNNGG 1 cut(s) 876
BssMI GATC 5 cut(s) 168, 193, 582, 958, 1072
BssNI GRCGYC 2 cut(s) 12, 183
BssSI CACGAG 1 cut(s) 471
BssT1I CCWWGG 1 cut(s) 876
Bst2BI CACGAG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 3 cut(s) 104, 654, 884
Bst6I CTCTTC 2 cut(s) 876, 903
BstACI GRCGYC 2 cut(s) 12, 183
BstC8I GCNNGC 2 cut(s) 347, 690
BstDEI CTNAG 4 cut(s) 19, 61, 352, 588
BstDSI CCRYGG 1 cut(s) 876
BstEII GGTNACC 1 cut(s) 739
BstF5I GGATG 5 cut(s) 127, 252, 257, 329, 498
BstFNI CGCG 2 cut(s) 71, 462
BstH2I RGCGCY 2 cut(s) 15, 293
BstHHI GCGC 3 cut(s) 14, 292, 462
BstKTI GATC 5 cut(s) 171, 196, 585, 961, 1075
BstMAI GTCTC 3 cut(s) 378, 897, 1028
BstMBI GATC 5 cut(s) 168, 193, 582, 958, 1072
BstMCI CGRYCG 2 cut(s) 279, 438
BstMWI GCNNNNNNNGC 3 cut(s) 20, 74, 275
BstNI CCWGG 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 813
BstPI GGTNACC 1 cut(s) 739
BstSCI CCNGG 2 cut(s) 186, 560
BstUI CGCG 2 cut(s) 71, 462
BstV1I GCAGC 3 cut(s) 495, 830, 923
BstV2I GAAGAC 3 cut(s) 98, 369, 1035
BstX2I RGATCY 2 cut(s) 582, 1072
BstYI RGATCY 2 cut(s) 582, 1072
BstZI CGGCCG 1 cut(s) 276
Bsu15I ATCGAT 1 cut(s) 167
Bsu36I CCTNAGG 1 cut(s) 352
BsuRI GGCC 4 cut(s) 191, 278, 349, 802
BsuTUI ATCGAT 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 876
BtsCI GGATG 5 cut(s) 127, 252, 257, 329, 498
BtsIMutI CAGTG 2 cut(s) 810, 1076
BveI ACCTGC 1 cut(s) 712
Cac8I GCNNGC 2 cut(s) 347, 690
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 3 cut(s) 14, 292, 462
Cfr13I GGNCC 1 cut(s) 348
ClaI ATCGAT 1 cut(s) 167
CseI GACGC 1 cut(s) 977
Csp6I GTAC 3 cut(s) 655, 679, 885
CviAII CATG 6 cut(s) 52, 574, 736, 810, 877, 1052
CviQI GTAC 3 cut(s) 655, 679, 885
DdeI CTNAG 4 cut(s) 19, 61, 352, 588
DinI GGCGCC 1 cut(s) 13
DpnI GATC 5 cut(s) 170, 195, 584, 960, 1074
DpnII GATC 5 cut(s) 168, 193, 582, 958, 1072
DraIII CACNNNGTG 1 cut(s) 156
DriI GACNNNNNGTC 1 cut(s) 621
EaeI YGGCCR 1 cut(s) 276
EagI CGGCCG 1 cut(s) 276
Eam1104I CTCTTC 2 cut(s) 876, 903
Eam1105I GACNNNNNGTC 1 cut(s) 621
EarI CTCTTC 2 cut(s) 876, 903
EciI GGCGGA 1 cut(s) 707
EclXI CGGCCG 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 876
Eco32I GATATC 1 cut(s) 1088
Eco47III AGCGCT 1 cut(s) 291
Eco52I CGGCCG 1 cut(s) 276
Eco81I CCTNAGG 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 88
Eco91I GGTNACC 1 cut(s) 739
EcoO65I GGTNACC 1 cut(s) 739
EcoRII CCWGG 1 cut(s) 186
EcoRV GATATC 1 cut(s) 1088
EcoT14I CCWWGG 1 cut(s) 876
EgeI GGCGCC 1 cut(s) 13
EheI GGCGCC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 876
Esp3I CGTCTC 1 cut(s) 378
FaeI CATG 6 cut(s) 55, 577, 739, 813, 880, 1055
FaqI GGGAC 2 cut(s) 109, 320
FatI CATG 6 cut(s) 51, 573, 735, 809, 876, 1051
FauI CCCGC 2 cut(s) 319, 613
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 6 cut(s) 10, 69, 139, 509, 844, 912
FokI GGATG 5 cut(s) 114, 239, 244, 316, 505
Fsp4HI GCNGC 6 cut(s) 10, 69, 139, 509, 844, 912
FspBI CTAG 2 cut(s) 542, 972
GlaI GCGC 3 cut(s) 13, 291, 461
GluI GCNGC 6 cut(s) 10, 69, 139, 509, 844, 912
HaeII RGCGCY 2 cut(s) 15, 293
HaeIII GGCC 4 cut(s) 191, 278, 349, 802
HapII CCGG 2 cut(s) 562, 699
HgaI GACGC 1 cut(s) 977
HhaI GCGC 3 cut(s) 14, 292, 462
Hin1I GRCGYC 2 cut(s) 12, 183
Hin1II CATG 6 cut(s) 55, 577, 739, 813, 880, 1055
Hin6I GCGC 3 cut(s) 12, 290, 460
HinP1I GCGC 3 cut(s) 12, 290, 460
HincII GTYRAC 2 cut(s) 435, 444
HindII GTYRAC 2 cut(s) 435, 444
HinfI GANTC 9 cut(s) 281, 390, 398, 413, 431, 695, 728, 824, 868
HpaII CCGG 2 cut(s) 562, 699
HphI GGTGA 5 cut(s) 135, 232, 407, 761, 877
Hpy166II GTNNAC 5 cut(s) 57, 161, 435, 444, 468
Hpy188I TCNGA 7 cut(s) 64, 181, 286, 534, 589, 665, 675
Hpy188III TCNNGA 4 cut(s) 88, 1025, 1052, 1096
Hpy8I GTNNAC 5 cut(s) 57, 161, 435, 444, 468
Hpy99I CGWCG 4 cut(s) 185, 371, 374, 971
HpyAV CCTTC 4 cut(s) 317, 792, 947, 996
HpyCH4III ACNGT 3 cut(s) 104, 654, 884
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 3 cut(s) 4, 846, 911
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 74, 275
HpyF3I CTNAG 4 cut(s) 19, 61, 352, 588
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 2 cut(s) 12, 183
Hsp92II CATG 6 cut(s) 55, 577, 739, 813, 880, 1055
HspAI GCGC 3 cut(s) 12, 290, 460
KasI GGCGCC 1 cut(s) 11
Kzo9I GATC 5 cut(s) 168, 193, 582, 958, 1072
LmnI GCTCC 1 cut(s) 99
Lsp1109I GCAGC 3 cut(s) 495, 830, 923
LweI GCATC 2 cut(s) 55, 70
MaeI CTAG 2 cut(s) 542, 972
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 5 cut(s) 648, 714, 739, 749, 865
MalI GATC 5 cut(s) 170, 195, 584, 960, 1074
MbiI CCGCTC 1 cut(s) 141
MboI GATC 5 cut(s) 168, 193, 582, 958, 1072
MboII GAAGA 8 cut(s) 98, 223, 374, 796, 863, 890, 893, 1040
MflI RGATCY 2 cut(s) 582, 1072
MluCI AATT 2 cut(s) 906, 1103
Mly113I GGCGCC 1 cut(s) 12
MlyI GAGTC 7 cut(s) 290, 384, 407, 407, 440, 818, 862
MmeI TCCRAC 2 cut(s) 66, 204
MseI TTAA 3 cut(s) 449, 1011, 1108
MslI CAYNNNNRTG 1 cut(s) 851
MspI CCGG 2 cut(s) 562, 699
MspR9I CCNGG 2 cut(s) 188, 562
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 2 cut(s) 71, 462
MwoI GCNNNNNNNGC 3 cut(s) 20, 74, 275
NarI GGCGCC 1 cut(s) 12
NciI CCSGG 1 cut(s) 562
NcoI CCATGG 1 cut(s) 876
NdeII GATC 5 cut(s) 168, 193, 582, 958, 1072
NlaIII CATG 6 cut(s) 55, 577, 739, 813, 880, 1055
NlaIV GGNNCC 2 cut(s) 13, 95
NmeAIII GCCGAG 2 cut(s) 254, 304
NmuCI GTSAC 2 cut(s) 749, 865
NspI RCATGY 1 cut(s) 813
PaeR7I CTCGAG 1 cut(s) 88
PagI TCATGA 1 cut(s) 1051
PaqCI CACCTGC 1 cut(s) 712
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PfeI GAWTC 2 cut(s) 695, 728
PflMI CCANNNNNTGG 2 cut(s) 345, 579
PkrI GCNGC 6 cut(s) 11, 70, 140, 510, 845, 913
PleI GAGTC 7 cut(s) 289, 384, 406, 407, 439, 818, 862
PluTI GGCGCC 1 cut(s) 15
PpsI GAGTC 7 cut(s) 289, 384, 406, 407, 439, 818, 862
Psp6I CCWGG 1 cut(s) 186
PspEI GGTNACC 1 cut(s) 739
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 2 cut(s) 13, 95
PspPI GGNCC 1 cut(s) 348
PsuI RGATCY 2 cut(s) 582, 1072
RsaI GTAC 3 cut(s) 656, 680, 886
RsaNI GTAC 3 cut(s) 655, 679, 885
RseI CAYNNNNRTG 1 cut(s) 851
SalI GTCGAC 1 cut(s) 433
SaqAI TTAA 3 cut(s) 449, 1011, 1108
SatI GCNGC 6 cut(s) 10, 69, 139, 509, 844, 912
Sau3AI GATC 5 cut(s) 168, 193, 582, 958, 1072
Sau96I GGNCC 1 cut(s) 348
ScaI AGTACT 1 cut(s) 680
SchI GAGTC 7 cut(s) 290, 384, 407, 407, 440, 818, 862
ScrFI CCNGG 2 cut(s) 188, 562
SfaNI GCATC 2 cut(s) 55, 70
SfoI GGCGCC 1 cut(s) 13
Sfr274I CTCGAG 1 cut(s) 88
SlaI CTCGAG 1 cut(s) 88
SmiMI CAYNNNNRTG 1 cut(s) 851
SmlI CTYRAG 2 cut(s) 88, 759
SmoI CTYRAG 2 cut(s) 88, 759
Sse9I AATT 2 cut(s) 906, 1103
SsiI CCGC 7 cut(s) 9, 69, 139, 312, 620, 692, 719
SspDI GGCGCC 1 cut(s) 11
SspMI CTAG 2 cut(s) 542, 972
StyD4I CCNGG 2 cut(s) 186, 560
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 3 cut(s) 104, 654, 884
TaiI ACGT 1 cut(s) 186
TaqI TCGA 7 cut(s) 89, 167, 303, 434, 504, 966, 1026
TaqII GACCGA 1 cut(s) 452
TasI AATT 2 cut(s) 906, 1103
TatI WGTACW 2 cut(s) 678, 884
TauI GCSGC 3 cut(s) 12, 71, 141
TfiI GAWTC 2 cut(s) 695, 728
Tru1I TTAA 3 cut(s) 449, 1011, 1108
Tru9I TTAA 3 cut(s) 449, 1011, 1108
TscAI CASTG 2 cut(s) 810, 1083
TseFI GTSAC 2 cut(s) 749, 865
TseI GCWGC 3 cut(s) 508, 843, 911
Tsp45I GTSAC 2 cut(s) 749, 865
TspDTI ATGAA 2 cut(s) 966, 1068
TspGWI ACGGA 3 cut(s) 322, 396, 402
TspRI CASTG 2 cut(s) 810, 1083
Van91I CCANNNNNTGG 2 cut(s) 345, 579
XceI RCATGY 1 cut(s) 813
XhoI CTCGAG 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 434
XspI CTAG 2 cut(s) 542, 972
ZraI GACGTC 1 cut(s) 184
ZrmI AGTACT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.