Rroxscaffold_4G00286560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005293
Physical Location & Seq
Reverse (-)
7544031 .. 7545983
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286560.1

Sequence Viewer

Length: 1173 bp
ATGCACGGCGGCGCCACGTCAGCTCCGCCACCGACGCCGTCGGCCCAGCAGTCGGACAAACCCATGTCCACGTCAGCCGCTGCTGACGCCCTCTCGAGCATGCTCCACCGTCTTCCACCCACTCTCTCCCTCCCCAATCGCCGCGCACAGCCAGCCACGTGTCCTCCGGTTCCGGTGGTCTCTTTGTCCCAAGCTCGATCCAACCCTAGTCTCTTCTCCTCGAAACTCGGGTTCTTCCAGCTCACCGACCACTCCATCCCTCCCCAACTCGCTGACTCGGCCGAGTCGGAAGCGCTGTCGCTCTTTGACCTATCCAGGGAGAAGAAAGAAGCGTGTTTTCCGAGGAACTGGCCTGTTGGGTTTGAGGAAGGAGATGAAGTTGACGATGATGACGTGGACGGACTCGGCGAGTCGTTTTGTCTGGACGAGTCGTGTTATACCGAGTCTGCTGGGTTGTCCTTGGCTTCTCTAAGCGAGTTCAGTCGTGCTTTGGAGAAAGTAGGGTTGGAGGTGACTGAGCTGCTTTGTAGGTCAGTCGGGTTTGAAAGTCCTCTAGGGAAGGGTGACCCGAGTCCGACCCGGGTTTGTTCAATGATGTTAGTGGCGGAGGGTTTATCGGGCAAGAGTAAGACGCCGGTGGCGGGTCGGTTTTACCCGTATGTGGTAGGATTGCAGTATCAAATCAGGGGTGGCCAGAAGTATTCTTTGCTGGCGGACTCGGGTTGGGTCTCGGTGGTGCCACTGGTGGGATCGGTCTTGGTCAGTATTGGTGACATTGCTCAAGTATGGAGCAATGGGGAGTTGAAGAAAGTGAGAGGAAGGCCAGTGGCAGGTTTGGGAGAGGGTAACAACAAGTCACGGTGCATATCGATGTCGTTGTTGGTGACTCTTCCTATCGACACTAAAATTTCCCCTGTTCTTCCGGCAGCAGCCATAAGCGGTGGTGATCACAGTCACAAGGAGGATGATAATGATGAAGACGGAAATGGAAGAAATGTTGATGACCTGGGAGATCATCAAATCAATGATAGCAGTATAGTCAGTACTGGTGAAAATAGAGAAATTGAAGGAAGGTTGTTCAAGTCCTTTTGTTTGGAAGACTATGCTTGGAGAGTGTATCATGAGCGCCTGCATTTCAAAGACCCGCTCGATAGGTTTCGCATTAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

390

Amino Acids

42.08

Weight (kDa)

5.03

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 75 - 119 2e-06 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 821
AccB1I GGYRCC 2 cut(s) 11, 736
AccBSI CCGCTC 1 cut(s) 1147
AccII CGCG 1 cut(s) 144
AciI CCGC 9 cut(s) 9, 26, 78, 142, 605, 641, 713, 939, 1145
AclWI GGATC 2 cut(s) 192, 757
AcoI YGGCCR 2 cut(s) 279, 691
AcsI RAATTY 1 cut(s) 906
AcvI CACGTG 1 cut(s) 159
AcyI GRCGYC 4 cut(s) 12, 35, 87, 632
AfaI GTAC 1 cut(s) 1045
AfeI AGCGCT 1 cut(s) 294
AfiI CCNNNNNNNGG 6 cut(s) 52, 316, 641, 661, 746, 830
AflIII ACRYGT 1 cut(s) 158
AgsI TTSAA 6 cut(s) 545, 591, 805, 1067, 1081, 1138
AjiI CACGTC 3 cut(s) 18, 72, 394
AjnI CCWGG 2 cut(s) 314, 1005
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AluBI AGCT 4 cut(s) 23, 194, 241, 520
AluI AGCT 4 cut(s) 23, 194, 241, 520
Alw26I GTCTC 3 cut(s) 184, 215, 733
AlwI GGATC 2 cut(s) 192, 757
AlwNI CAGNNNCTG 1 cut(s) 80
Ama87I CYCGRG 5 cut(s) 94, 227, 568, 579, 718
Aor51HI AGCGCT 1 cut(s) 294
AoxI GGCC 5 cut(s) 42, 279, 350, 691, 821
ApeKI GCWGC 4 cut(s) 80, 520, 926, 929
ApoI RAATTY 1 cut(s) 906
AspLEI GCGC 4 cut(s) 14, 146, 295, 1128
AspS9I GGNCC 1 cut(s) 43
AsuC2I CCSGG 2 cut(s) 580, 581
AsuHPI GGTGA 7 cut(s) 235, 523, 575, 782, 895, 956, 1061
AvaI CYCGRG 5 cut(s) 94, 227, 568, 579, 718
BalI TGGCCA 1 cut(s) 693
BanI GGYRCC 2 cut(s) 11, 736
BbrPI CACGTG 1 cut(s) 159
BbsI GAAGAC 3 cut(s) 104, 984, 1104
BbvI GCAGC 4 cut(s) 67, 507, 938, 941
BccI CCATC 1 cut(s) 263
BceAI ACGGC 2 cut(s) 22, 22
BciT130I CCWGG 2 cut(s) 316, 1007
BclI TGATCA 1 cut(s) 946
BcnI CCSGG 2 cut(s) 580, 581
BcoDI GTCTC 3 cut(s) 184, 215, 733
BfaI CTAG 2 cut(s) 207, 554
BfoI RGCGCY 3 cut(s) 15, 296, 1129
BfuAI ACCTGC 1 cut(s) 821
BisI GCNGC 7 cut(s) 10, 78, 81, 142, 521, 927, 930
BlsI GCNGC 7 cut(s) 11, 79, 82, 143, 522, 928, 931
BmcAI AGTACT 1 cut(s) 1045
Bme1390I CCNGG 4 cut(s) 316, 580, 581, 1007
BmeT110I CYCGRG 5 cut(s) 94, 227, 568, 579, 718
BmgBI CACGTC 3 cut(s) 18, 72, 394
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 3 cut(s) 13, 171, 738
BmrFI CCNGG 4 cut(s) 316, 580, 581, 1007
BoxI GACNNNNGTC 1 cut(s) 570
BpiI GAAGAC 3 cut(s) 104, 984, 1104
BpuEI CTTGAG 1 cut(s) 765
BpuMI CCSGG 2 cut(s) 580, 581
Bsa29I ATCGAT 1 cut(s) 869
BsaAI YACGTR 1 cut(s) 159
BsaHI GRCGYC 4 cut(s) 12, 35, 87, 632
BsaI GGTCTC 2 cut(s) 184, 733
BsaJI CCNNGG 5 cut(s) 315, 341, 459, 579, 1006
BsaWI WCCGGW 2 cut(s) 166, 172
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 148, 178, 363, 393
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 316, 641, 661, 746, 830
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 4 cut(s) 353, 747, 824, 1051
Bse3DI GCAATG 2 cut(s) 774, 799
BseBI CCWGG 2 cut(s) 316, 1007
BseCI ATCGAT 1 cut(s) 869
BseDI CCNNGG 5 cut(s) 315, 341, 459, 579, 1006
BseGI GGATG 2 cut(s) 255, 970
BseLI CCNNNNNNNGG 6 cut(s) 52, 316, 641, 661, 746, 830
BseMI GCAATG 2 cut(s) 774, 799
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 4 cut(s) 353, 747, 824, 1051
BseRI GAGGAG 1 cut(s) 208
BseX3I CGGCCG 1 cut(s) 279
BseXI GCAGC 4 cut(s) 67, 507, 938, 941
BseYI CCCAGC 2 cut(s) 45, 449
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 282
BshFI GGCC 5 cut(s) 44, 281, 352, 693, 823
BshNI GGYRCC 2 cut(s) 11, 736
BshVI ATCGAT 1 cut(s) 869
BsiEI CGRYCG 1 cut(s) 282
BsiHKCI CYCGRG 5 cut(s) 94, 227, 568, 579, 718
BsiSI CCGG 5 cut(s) 167, 173, 580, 635, 923
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 6 cut(s) 52, 316, 641, 661, 746, 830
BsmAI GTCTC 3 cut(s) 184, 215, 733
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 5 cut(s) 44, 281, 352, 693, 823
Bso31I GGTCTC 2 cut(s) 184, 733
BsoBI CYCGRG 5 cut(s) 94, 227, 568, 579, 718
Bsp143I GATC 4 cut(s) 197, 749, 946, 1012
BspACI CCGC 9 cut(s) 9, 26, 78, 142, 605, 641, 713, 939, 1145
BspANI GGCC 5 cut(s) 44, 281, 352, 693, 823
BspCNI CTCAG 1 cut(s) 508
BspDI ATCGAT 1 cut(s) 869
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 1120
BspLI GGNNCC 3 cut(s) 13, 171, 738
BspMI ACCTGC 1 cut(s) 821
BspPI GGATC 2 cut(s) 192, 757
BspT107I GGYRCC 2 cut(s) 11, 736
BspTNI GGTCTC 2 cut(s) 184, 733
BsrBI CCGCTC 1 cut(s) 1147
BsrDI GCAATG 2 cut(s) 774, 799
BsrFI RCCGGY 1 cut(s) 634
BsrI ACTGG 4 cut(s) 353, 747, 824, 1051
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 5 cut(s) 315, 341, 459, 579, 1006
BssMI GATC 4 cut(s) 197, 749, 946, 1012
BssNI GRCGYC 4 cut(s) 12, 35, 87, 632
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 2 cut(s) 316, 1007
Bst4CI ACNGT 3 cut(s) 110, 861, 953
Bst6I CTCTTC 2 cut(s) 218, 894
BstACI GRCGYC 4 cut(s) 12, 35, 87, 632
BstBAI YACGTR 1 cut(s) 159
BstC8I GCNNGC 4 cut(s) 101, 153, 711, 1130
BstDEI CTNAG 2 cut(s) 470, 516
BstEII GGTNACC 1 cut(s) 563
BstF5I GGATG 2 cut(s) 255, 970
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 3 cut(s) 15, 296, 1129
BstHHI GCGC 4 cut(s) 14, 146, 295, 1128
BstKTI GATC 4 cut(s) 200, 752, 949, 1015
BstMAI GTCTC 3 cut(s) 184, 215, 733
BstMBI GATC 4 cut(s) 197, 749, 946, 1012
BstMCI CGRYCG 1 cut(s) 282
BstMWI GCNNNNNNNGC 5 cut(s) 20, 34, 86, 152, 278
BstNI CCWGG 2 cut(s) 316, 1007
BstNSI RCATGY 1 cut(s) 103
BstPAI GACNNNNGTC 1 cut(s) 570
BstPI GGTNACC 1 cut(s) 563
BstSCI CCNGG 4 cut(s) 314, 578, 579, 1005
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 4 cut(s) 67, 507, 938, 941
BstV2I GAAGAC 3 cut(s) 104, 984, 1104
BstZI CGGCCG 1 cut(s) 279
Bsu15I ATCGAT 1 cut(s) 869
BsuRI GGCC 5 cut(s) 44, 281, 352, 693, 823
BsuTUI ATCGAT 1 cut(s) 869
BtrI CACGTC 3 cut(s) 18, 72, 394
BtsCI GGATG 2 cut(s) 255, 970
BtsIMutI CAGTG 2 cut(s) 740, 831
BveI ACCTGC 1 cut(s) 821
Cac8I GCNNGC 4 cut(s) 101, 153, 711, 1130
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 1 cut(s) 1120
CfoI GCGC 4 cut(s) 14, 146, 295, 1128
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 1 cut(s) 43
Cfr9I CCCGGG 1 cut(s) 579
ClaI ATCGAT 1 cut(s) 869
CseI GACGC 3 cut(s) 43, 95, 640
Csp6I GTAC 1 cut(s) 1044
CviAII CATG 3 cut(s) 64, 100, 1121
CviQI GTAC 1 cut(s) 1044
DdeI CTNAG 2 cut(s) 470, 516
DinI GGCGCC 1 cut(s) 13
DpnI GATC 4 cut(s) 199, 751, 948, 1014
DpnII GATC 4 cut(s) 197, 749, 946, 1012
EaeI YGGCCR 2 cut(s) 279, 691
EagI CGGCCG 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 218, 894
EarI CTCTTC 2 cut(s) 218, 894
EciI GGCGGA 3 cut(s) 15, 620, 728
EclXI CGGCCG 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 459
Eco31I GGTCTC 2 cut(s) 184, 733
Eco47III AGCGCT 1 cut(s) 294
Eco52I CGGCCG 1 cut(s) 279
Eco72I CACGTG 1 cut(s) 159
Eco88I CYCGRG 5 cut(s) 94, 227, 568, 579, 718
Eco91I GGTNACC 1 cut(s) 563
EcoO65I GGTNACC 1 cut(s) 563
EcoRII CCWGG 2 cut(s) 314, 1005
EcoT14I CCWWGG 1 cut(s) 459
EgeI GGCGCC 1 cut(s) 13
EheI GGCGCC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 3 cut(s) 67, 103, 1124
FaqI GGGAC 1 cut(s) 172
FatI CATG 3 cut(s) 63, 99, 1120
FauI CCCGC 2 cut(s) 634, 1152
FbaI TGATCA 1 cut(s) 946
Fnu4HI GCNGC 7 cut(s) 10, 78, 81, 142, 521, 927, 930
FokI GGATG 2 cut(s) 242, 977
Fsp4HI GCNGC 7 cut(s) 10, 78, 81, 142, 521, 927, 930
FspBI CTAG 2 cut(s) 207, 554
GlaI GCGC 4 cut(s) 13, 145, 294, 1127
GluI GCNGC 7 cut(s) 10, 78, 81, 142, 521, 927, 930
GsaI CCCAGC 2 cut(s) 49, 453
HaeII RGCGCY 3 cut(s) 15, 296, 1129
HaeIII GGCC 5 cut(s) 44, 281, 352, 693, 823
HapII CCGG 5 cut(s) 167, 173, 580, 635, 923
HgaI GACGC 3 cut(s) 43, 95, 640
HhaI GCGC 4 cut(s) 14, 146, 295, 1128
Hin1I GRCGYC 4 cut(s) 12, 35, 87, 632
Hin1II CATG 3 cut(s) 67, 103, 1124
Hin6I GCGC 4 cut(s) 12, 144, 293, 1126
HinP1I GCGC 4 cut(s) 12, 144, 293, 1126
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 9 cut(s) 275, 284, 402, 410, 428, 443, 571, 716, 886
HpaII CCGG 5 cut(s) 167, 173, 580, 635, 923
HphI GGTGA 7 cut(s) 235, 523, 575, 782, 895, 956, 1061
Hpy166II GTNNAC 3 cut(s) 69, 382, 397
Hpy188I TCNGA 4 cut(s) 55, 289, 342, 576
Hpy188III TCNNGA 3 cut(s) 94, 422, 1121
Hpy8I GTNNAC 3 cut(s) 69, 382, 397
Hpy99I CGWCG 2 cut(s) 37, 43
HpyAV CCTTC 5 cut(s) 362, 553, 813, 1061, 1065
HpyCH4III ACNGT 3 cut(s) 110, 861, 953
HpyCH4IV ACGT 4 cut(s) 17, 71, 158, 393
HpyCH4V TGCA 4 cut(s) 4, 673, 864, 1132
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 34, 86, 152, 278
HpyF3I CTNAG 2 cut(s) 470, 516
HpySE526I ACGT 4 cut(s) 17, 71, 158, 393
Hsp92I GRCGYC 4 cut(s) 12, 35, 87, 632
Hsp92II CATG 3 cut(s) 67, 103, 1124
HspAI GCGC 4 cut(s) 12, 144, 293, 1126
KasI GGCGCC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 946
Kzo9I GATC 4 cut(s) 197, 749, 946, 1012
LmnI GCTCC 3 cut(s) 28, 108, 789
Lsp1109I GCAGC 4 cut(s) 67, 507, 938, 941
MaeI CTAG 2 cut(s) 207, 554
MaeII ACGT 4 cut(s) 17, 71, 158, 393
MaeIII GTNAC 7 cut(s) 511, 563, 770, 845, 855, 883, 953
MalI GATC 4 cut(s) 199, 751, 948, 1014
MbiI CCGCTC 1 cut(s) 1147
MboI GATC 4 cut(s) 197, 749, 946, 1012
MlsI TGGCCA 1 cut(s) 693
MluCI AATT 3 cut(s) 906, 1062, 1168
MluNI TGGCCA 1 cut(s) 693
Mly113I GGCGCC 1 cut(s) 12
MlyI GAGTC 9 cut(s) 269, 293, 396, 419, 437, 452, 580, 710, 880
MmeI TCCRAC 5 cut(s) 33, 225, 267, 486, 599
Mox20I TGGCCA 1 cut(s) 693
MscI TGGCCA 1 cut(s) 693
MseI TTAA 1 cut(s) 1164
MslI CAYNNNNRTG 1 cut(s) 869
Msp20I TGGCCA 1 cut(s) 693
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 5 cut(s) 167, 173, 580, 635, 923
MspR9I CCNGG 4 cut(s) 316, 580, 581, 1007
MvaI CCWGG 2 cut(s) 316, 1007
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 5 cut(s) 20, 34, 86, 152, 278
NarI GGCGCC 1 cut(s) 12
NciI CCSGG 2 cut(s) 580, 581
NdeII GATC 4 cut(s) 197, 749, 946, 1012
NlaIII CATG 3 cut(s) 67, 103, 1124
NlaIV GGNNCC 3 cut(s) 13, 171, 738
NmeAIII GCCGAG 3 cut(s) 257, 307, 384
NmuCI GTSAC 6 cut(s) 511, 563, 770, 855, 883, 953
NspI RCATGY 1 cut(s) 103
PaeI GCATGC 1 cut(s) 103
PaeR7I CTCGAG 1 cut(s) 94
PagI TCATGA 1 cut(s) 1120
PcsI WCGNNNNNNNCGW 2 cut(s) 390, 405
PflFI GACNNNGTC 1 cut(s) 37
PkrI GCNGC 7 cut(s) 11, 79, 82, 143, 522, 928, 931
PleI GAGTC 9 cut(s) 269, 292, 396, 418, 436, 451, 579, 710, 880
PluTI GGCGCC 1 cut(s) 15
PmaCI CACGTG 1 cut(s) 159
PmlI CACGTG 1 cut(s) 159
PpsI GAGTC 9 cut(s) 269, 292, 396, 418, 436, 451, 579, 710, 880
Ppu21I YACGTR 1 cut(s) 159
PshAI GACNNNNGTC 1 cut(s) 570
Psp6I CCWGG 2 cut(s) 314, 1005
PspCI CACGTG 1 cut(s) 159
PspEI GGTNACC 1 cut(s) 563
PspFI CCCAGC 2 cut(s) 45, 449
PspGI CCWGG 2 cut(s) 314, 1005
PspN4I GGNNCC 3 cut(s) 13, 171, 738
PspPI GGNCC 1 cut(s) 43
PstNI CAGNNNCTG 1 cut(s) 80
PsyI GACNNNGTC 1 cut(s) 37
RsaI GTAC 1 cut(s) 1045
RsaNI GTAC 1 cut(s) 1044
RseI CAYNNNNRTG 1 cut(s) 869
SaqAI TTAA 1 cut(s) 1164
SatI GCNGC 7 cut(s) 10, 78, 81, 142, 521, 927, 930
Sau3AI GATC 4 cut(s) 197, 749, 946, 1012
Sau96I GGNCC 1 cut(s) 43
ScaI AGTACT 1 cut(s) 1045
SchI GAGTC 9 cut(s) 269, 293, 396, 419, 437, 452, 580, 710, 880
ScrFI CCNGG 4 cut(s) 316, 580, 581, 1007
SfoI GGCGCC 1 cut(s) 13
Sfr274I CTCGAG 1 cut(s) 94
SgrAI CRCCGGYG 1 cut(s) 634
SlaI CTCGAG 1 cut(s) 94
SmaI CCCGGG 1 cut(s) 581
SmiMI CAYNNNNRTG 1 cut(s) 869
SmlI CTYRAG 2 cut(s) 94, 780
SmoI CTYRAG 2 cut(s) 94, 780
SphI GCATGC 1 cut(s) 103
Sse9I AATT 3 cut(s) 906, 1062, 1168
SsiI CCGC 9 cut(s) 9, 26, 78, 142, 605, 641, 713, 939, 1145
SspDI GGCGCC 1 cut(s) 11
SspMI CTAG 2 cut(s) 207, 554
StyD4I CCNGG 4 cut(s) 314, 578, 579, 1005
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 3 cut(s) 110, 861, 953
TaiI ACGT 4 cut(s) 20, 74, 161, 396
TaqI TCGA 6 cut(s) 95, 196, 221, 869, 897, 1149
TaqII GACCGA 1 cut(s) 742
TasI AATT 3 cut(s) 906, 1062, 1168
TatI WGTACW 1 cut(s) 1043
TauI GCSGC 3 cut(s) 12, 80, 144
Tru1I TTAA 1 cut(s) 1164
Tru9I TTAA 1 cut(s) 1164
TscAI CASTG 2 cut(s) 747, 831
TseFI GTSAC 6 cut(s) 511, 563, 770, 855, 883, 953
TseI GCWGC 4 cut(s) 80, 520, 926, 929
Tsp45I GTSAC 6 cut(s) 511, 563, 770, 855, 883, 953
TspDTI ATGAA 2 cut(s) 390, 990
TspGWI ACGGA 2 cut(s) 414, 996
TspMI CCCGGG 1 cut(s) 579
TspRI CASTG 2 cut(s) 747, 831
Tth111I GACNNNGTC 1 cut(s) 37
XapI RAATTY 1 cut(s) 906
XceI RCATGY 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 94
XmaI CCCGGG 1 cut(s) 579
XspI CTAG 2 cut(s) 207, 554
ZrmI AGTACT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.