RLG00000027041

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005293
Physical Location & Seq
Reverse (-)
7871889 .. 7873481
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027041

Sequence Viewer

Length: 1167 bp
ATGCACGGCGGCGCGACGTCAGCTCCGCCACCGACGCCATCGACCCAGCAGTCGGACAAACCCATGTCCACGTCAGCCGCGGCTGACGCCCTCTCGAGCATGCTCCACCGTCTTCCACCCACTCTCTCCCTCCCCACTAGCCGCGCACGGCCAGCCACGTGTCCTCCGGTTCCGGTGGTCTCTTTGTCCCAAGCTCGATCCAACCCTAGTCTCTTCTCCCCGAAACTCGGGTTCTTCCAGCTGACCGACCACTCCATCCCTCCCCAACTCGCTGACTCGGCCGAGTCGGAAGCGCTGTCGCTCTTTGACCTACCCAGGGAGAAGAAAGAAGCGTGTTTTCCGAGGAACTGGCCTGTTGGGTTTGAAGAAGGAGATGAAGATGATGACGTGGAGGGACTCGGCGAGTCGTTTTGTCTGGACGAGTCGTGTTATACCGAGTCTGCTGGGTTGTCCTTGGCTTCTCTAAGCGAGTTCAGTCGTGCTTTGGAGAAAGTAGGGTTGGAGGTGACTGAGCTGCTTTGTTGGTCAGTCGGGTTTGAAAGTCCTCTAGGGAAGGGTGACCCGAGTCCGACCCGGGTTTGTTCGATGATGTGGGTGGCGGAGGGTTTATCGGGCAAGAGTAAGACGCCGGTGGCGGGTCGGTTTTACCCGTATGTGGTAGGATTGCAGTATCAAATCAGGGGTGGCCAGAAGTATTCTTTGCTGACGGATTCGGGTTGGGTCTCGGTGGTGCCACTGGTGGAATCGGTCTTGGTCACTATTGGTGACATTGCTCAAGTATGGAGCAATGGGGAGTTGAAGAAAGTGAGAGGAAGGCCAGTGGCAGGTTTGGGAGAGGGTAACAACAAGTCACGGTGCATATCGATGTCGTTGTTGGTGACTCTTCCTATCGACACTAAAATTTCCCCTGTTCTTCCGGCAGCAGCCATAAGCGGTGGTGATCACAGTCACAAGGATGATGATAATGATGAAGATGGAAATGGAAGAAATGTTGATGAACTGGGAGATCATCAAATCAATGATAGCAGTACAGTCAGTACTGGTGAAAATAGAGAAATTGAAGGAAGGTTGTTCAAGTCCTTTTGTCTGGAAGACTATGCTTGGAGAGTGTATCATGAACGGCTGCATTTCAAAGACCCGCTCGATAGGTTTCGCATTAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

389

Amino Acids

42.08

Weight (kDa)

4.99

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 75 - 115 5.1e-07 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 49
AatII GACGTC 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 730
AccBSI CCGCTC 1 cut(s) 1141
AccII CGCG 3 cut(s) 14, 80, 144
AciI CCGC 9 cut(s) 9, 26, 78, 80, 142, 599, 635, 933, 1139
AclWI GGATC 1 cut(s) 192
AcoI YGGCCR 3 cut(s) 149, 279, 685
AcsI RAATTY 1 cut(s) 900
AcvI CACGTG 1 cut(s) 159
AcyI GRCGYC 4 cut(s) 17, 35, 87, 626
AfaI GTAC 2 cut(s) 1030, 1039
AfeI AGCGCT 1 cut(s) 294
AfiI CCNNNNNNNGG 6 cut(s) 52, 227, 316, 635, 655, 824
AflIII ACRYGT 1 cut(s) 158
AgsI TTSAA 6 cut(s) 365, 539, 799, 1061, 1075, 1132
AjiI CACGTC 2 cut(s) 72, 388
AjnI CCWGG 1 cut(s) 314
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AluBI AGCT 4 cut(s) 23, 194, 241, 514
AluI AGCT 4 cut(s) 23, 194, 241, 514
Alw26I GTCTC 3 cut(s) 184, 215, 727
AlwI GGATC 1 cut(s) 192
Ama87I CYCGRG 4 cut(s) 94, 227, 562, 573
Aor51HI AGCGCT 1 cut(s) 294
AoxI GGCC 5 cut(s) 149, 279, 350, 685, 815
ApeKI GCWGC 4 cut(s) 514, 920, 923, 1123
ApoI RAATTY 1 cut(s) 900
AspLEI GCGC 3 cut(s) 14, 146, 295
AsuC2I CCSGG 2 cut(s) 574, 575
AsuHPI GGTGA 6 cut(s) 517, 569, 776, 889, 950, 1055
AvaI CYCGRG 4 cut(s) 94, 227, 562, 573
BalI TGGCCA 1 cut(s) 687
BanI GGYRCC 1 cut(s) 730
BbrPI CACGTG 1 cut(s) 159
BbsI GAAGAC 2 cut(s) 104, 1098
BbvI GCAGC 4 cut(s) 501, 932, 935, 1110
BccI CCATC 3 cut(s) 46, 263, 968
BceAI ACGGC 3 cut(s) 22, 164, 1136
BciT130I CCWGG 1 cut(s) 316
BclI TGATCA 1 cut(s) 940
BcnI CCSGG 2 cut(s) 574, 575
BcoDI GTCTC 3 cut(s) 184, 215, 727
BfaI CTAG 3 cut(s) 138, 207, 548
BfoI RGCGCY 1 cut(s) 296
BfuAI ACCTGC 1 cut(s) 815
BisI GCNGC 8 cut(s) 10, 78, 81, 142, 515, 921, 924, 1124
BlsI GCNGC 8 cut(s) 11, 79, 82, 143, 516, 922, 925, 1125
BmcAI AGTACT 1 cut(s) 1039
Bme1390I CCNGG 3 cut(s) 316, 574, 575
BmeT110I CYCGRG 4 cut(s) 94, 227, 562, 573
BmgBI CACGTC 2 cut(s) 72, 388
BmiI GGNNCC 2 cut(s) 171, 732
BmrFI CCNGG 3 cut(s) 316, 574, 575
BmrI ACTGGG 1 cut(s) 1010
BmuI ACTGGG 1 cut(s) 1010
BoxI GACNNNNGTC 1 cut(s) 564
BpiI GAAGAC 2 cut(s) 104, 1098
BpuEI CTTGAG 1 cut(s) 759
BpuMI CCSGG 2 cut(s) 574, 575
Bsa29I ATCGAT 1 cut(s) 863
BsaAI YACGTR 1 cut(s) 159
BsaHI GRCGYC 4 cut(s) 17, 35, 87, 626
BsaI GGTCTC 2 cut(s) 184, 727
BsaJI CCNNGG 6 cut(s) 78, 314, 315, 341, 453, 573
BsaWI WCCGGW 2 cut(s) 166, 172
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 148, 178
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 227, 316, 635, 655, 824
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 5 cut(s) 353, 741, 818, 1005, 1045
Bse3DI GCAATG 2 cut(s) 768, 793
BseBI CCWGG 1 cut(s) 316
BseCI ATCGAT 1 cut(s) 863
BseDI CCNNGG 6 cut(s) 78, 314, 315, 341, 453, 573
BseGI GGATG 2 cut(s) 255, 961
BseLI CCNNNNNNNGG 6 cut(s) 52, 227, 316, 635, 655, 824
BseMI GCAATG 2 cut(s) 768, 793
BseMII CTCAG 1 cut(s) 501
BseNI ACTGG 5 cut(s) 353, 741, 818, 1005, 1045
BseX3I CGGCCG 1 cut(s) 279
BseXI GCAGC 4 cut(s) 501, 932, 935, 1110
BseYI CCCAGC 2 cut(s) 45, 443
Bsh1236I CGCG 3 cut(s) 14, 80, 144
Bsh1285I CGRYCG 1 cut(s) 282
BshFI GGCC 5 cut(s) 151, 281, 352, 687, 817
BshNI GGYRCC 1 cut(s) 730
BshVI ATCGAT 1 cut(s) 863
BsiEI CGRYCG 1 cut(s) 282
BsiHKCI CYCGRG 4 cut(s) 94, 227, 562, 573
BsiSI CCGG 5 cut(s) 167, 173, 574, 629, 917
BslFI GGGAC 2 cut(s) 172, 408
BslI CCNNNNNNNGG 6 cut(s) 52, 227, 316, 635, 655, 824
BsmAI GTCTC 3 cut(s) 184, 215, 727
BsmFI GGGAC 2 cut(s) 172, 408
BsnI GGCC 5 cut(s) 151, 281, 352, 687, 817
Bso31I GGTCTC 2 cut(s) 184, 727
BsoBI CYCGRG 4 cut(s) 94, 227, 562, 573
Bsp143I GATC 3 cut(s) 197, 940, 1006
BspACI CCGC 9 cut(s) 9, 26, 78, 80, 142, 599, 635, 933, 1139
BspANI GGCC 5 cut(s) 151, 281, 352, 687, 817
BspCNI CTCAG 1 cut(s) 502
BspDI ATCGAT 1 cut(s) 863
BspFNI CGCG 3 cut(s) 14, 80, 144
BspHI TCATGA 1 cut(s) 1114
BspLI GGNNCC 2 cut(s) 171, 732
BspMI ACCTGC 1 cut(s) 815
BspPI GGATC 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 730
BspTNI GGTCTC 2 cut(s) 184, 727
BsrBI CCGCTC 1 cut(s) 1141
BsrDI GCAATG 2 cut(s) 768, 793
BsrFI RCCGGY 1 cut(s) 628
BsrI ACTGG 5 cut(s) 353, 741, 818, 1005, 1045
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 6 cut(s) 78, 314, 315, 341, 453, 573
BssMI GATC 3 cut(s) 197, 940, 1006
BssNI GRCGYC 4 cut(s) 17, 35, 87, 626
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 316
Bst4CI ACNGT 4 cut(s) 110, 855, 947, 1033
Bst6I CTCTTC 2 cut(s) 218, 888
BstACI GRCGYC 4 cut(s) 17, 35, 87, 626
BstBAI YACGTR 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 101, 153
BstDEI CTNAG 2 cut(s) 464, 510
BstDSI CCRYGG 1 cut(s) 78
BstEII GGTNACC 1 cut(s) 557
BstF5I GGATG 2 cut(s) 255, 961
BstFNI CGCG 3 cut(s) 14, 80, 144
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 3 cut(s) 14, 146, 295
BstKTI GATC 3 cut(s) 200, 943, 1009
BstMAI GTCTC 3 cut(s) 184, 215, 727
BstMBI GATC 3 cut(s) 197, 940, 1006
BstMCI CGRYCG 1 cut(s) 282
BstMWI GCNNNNNNNGC 5 cut(s) 20, 34, 86, 152, 278
BstNI CCWGG 1 cut(s) 316
BstNSI RCATGY 1 cut(s) 103
BstPAI GACNNNNGTC 1 cut(s) 564
BstPI GGTNACC 1 cut(s) 557
BstSCI CCNGG 3 cut(s) 314, 572, 573
BstUI CGCG 3 cut(s) 14, 80, 144
BstV1I GCAGC 4 cut(s) 501, 932, 935, 1110
BstV2I GAAGAC 2 cut(s) 104, 1098
BstZI CGGCCG 1 cut(s) 279
Bsu15I ATCGAT 1 cut(s) 863
BsuRI GGCC 5 cut(s) 151, 281, 352, 687, 817
BsuTUI ATCGAT 1 cut(s) 863
BtgI CCRYGG 1 cut(s) 78
BtrI CACGTC 2 cut(s) 72, 388
BtsCI GGATG 2 cut(s) 255, 961
BtsIMutI CAGTG 2 cut(s) 734, 825
BveI ACCTGC 1 cut(s) 815
Cac8I GCNNGC 2 cut(s) 101, 153
CciI TCATGA 1 cut(s) 1114
CfoI GCGC 3 cut(s) 14, 146, 295
Cfr10I RCCGGY 1 cut(s) 628
Cfr42I CCGCGG 1 cut(s) 81
Cfr9I CCCGGG 1 cut(s) 573
ClaI ATCGAT 1 cut(s) 863
CseI GACGC 3 cut(s) 43, 95, 634
Csp6I GTAC 2 cut(s) 1029, 1038
CviAII CATG 3 cut(s) 64, 100, 1115
CviQI GTAC 2 cut(s) 1029, 1038
DdeI CTNAG 2 cut(s) 464, 510
DpnI GATC 3 cut(s) 199, 942, 1008
DpnII GATC 3 cut(s) 197, 940, 1006
DrdI GACNNNNNNGTC 1 cut(s) 49
DseDI GACNNNNNNGTC 1 cut(s) 49
EaeI YGGCCR 3 cut(s) 149, 279, 685
EagI CGGCCG 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 218, 888
EarI CTCTTC 2 cut(s) 218, 888
EciI GGCGGA 2 cut(s) 15, 614
EclXI CGGCCG 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 453
Eco31I GGTCTC 2 cut(s) 184, 727
Eco47III AGCGCT 1 cut(s) 294
Eco52I CGGCCG 1 cut(s) 279
Eco72I CACGTG 1 cut(s) 159
Eco88I CYCGRG 4 cut(s) 94, 227, 562, 573
Eco91I GGTNACC 1 cut(s) 557
EcoO65I GGTNACC 1 cut(s) 557
EcoRII CCWGG 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 3 cut(s) 67, 103, 1118
FaiI YATR 9 cut(s) 65, 101, 432, 654, 781, 860, 929, 1098, 1116
FaqI GGGAC 2 cut(s) 172, 408
FatI CATG 3 cut(s) 63, 99, 1114
FauI CCCGC 2 cut(s) 628, 1146
FbaI TGATCA 1 cut(s) 940
Fnu4HI GCNGC 8 cut(s) 10, 78, 81, 142, 515, 921, 924, 1124
FokI GGATG 2 cut(s) 242, 968
Fsp4HI GCNGC 8 cut(s) 10, 78, 81, 142, 515, 921, 924, 1124
FspBI CTAG 3 cut(s) 138, 207, 548
GlaI GCGC 3 cut(s) 13, 145, 294
GluI GCNGC 8 cut(s) 10, 78, 81, 142, 515, 921, 924, 1124
GsaI CCCAGC 2 cut(s) 49, 447
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 5 cut(s) 151, 281, 352, 687, 817
HapII CCGG 5 cut(s) 167, 173, 574, 629, 917
HgaI GACGC 3 cut(s) 43, 95, 634
HhaI GCGC 3 cut(s) 14, 146, 295
Hin1I GRCGYC 4 cut(s) 17, 35, 87, 626
Hin1II CATG 3 cut(s) 67, 103, 1118
Hin6I GCGC 3 cut(s) 12, 144, 293
HinP1I GCGC 3 cut(s) 12, 144, 293
HpaII CCGG 5 cut(s) 167, 173, 574, 629, 917
HphI GGTGA 6 cut(s) 517, 569, 776, 889, 950, 1055
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 4 cut(s) 55, 289, 342, 570
Hpy188III TCNNGA 4 cut(s) 94, 416, 1088, 1115
Hpy8I GTNNAC 1 cut(s) 69
Hpy99I CGWCG 2 cut(s) 19, 37
HpyAV CCTTC 5 cut(s) 362, 547, 807, 1055, 1059
HpyCH4III ACNGT 4 cut(s) 110, 855, 947, 1033
HpyCH4IV ACGT 4 cut(s) 17, 71, 158, 387
HpyCH4V TGCA 4 cut(s) 4, 667, 858, 1126
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 34, 86, 152, 278
HpyF3I CTNAG 2 cut(s) 464, 510
HpySE526I ACGT 4 cut(s) 17, 71, 158, 387
Hsp92I GRCGYC 4 cut(s) 17, 35, 87, 626
Hsp92II CATG 3 cut(s) 67, 103, 1118
HspAI GCGC 3 cut(s) 12, 144, 293
Ksp22I TGATCA 1 cut(s) 940
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 3 cut(s) 197, 940, 1006
LmnI GCTCC 3 cut(s) 28, 108, 783
Lsp1109I GCAGC 4 cut(s) 501, 932, 935, 1110
MaeI CTAG 3 cut(s) 138, 207, 548
MaeII ACGT 4 cut(s) 17, 71, 158, 387
MaeIII GTNAC 8 cut(s) 505, 557, 754, 764, 839, 849, 877, 947
MalI GATC 3 cut(s) 199, 942, 1008
MbiI CCGCTC 1 cut(s) 1141
MboI GATC 3 cut(s) 197, 940, 1006
MlsI TGGCCA 1 cut(s) 687
MluCI AATT 2 cut(s) 900, 1056
MluNI TGGCCA 1 cut(s) 687
MlyI GAGTC 8 cut(s) 269, 293, 390, 413, 431, 446, 574, 874
MmeI TCCRAC 5 cut(s) 33, 225, 267, 480, 593
Mox20I TGGCCA 1 cut(s) 687
MscI TGGCCA 1 cut(s) 687
MseI TTAA 1 cut(s) 1158
MslI CAYNNNNRTG 2 cut(s) 863, 954
Msp20I TGGCCA 1 cut(s) 687
MspA1I CMGCKG 2 cut(s) 80, 241
MspI CCGG 5 cut(s) 167, 173, 574, 629, 917
MspR9I CCNGG 3 cut(s) 316, 574, 575
MvaI CCWGG 1 cut(s) 316
MvnI CGCG 3 cut(s) 14, 80, 144
MwoI GCNNNNNNNGC 5 cut(s) 20, 34, 86, 152, 278
NciI CCSGG 2 cut(s) 574, 575
NdeII GATC 3 cut(s) 197, 940, 1006
NlaIII CATG 3 cut(s) 67, 103, 1118
NlaIV GGNNCC 2 cut(s) 171, 732
NmeAIII GCCGAG 3 cut(s) 257, 307, 378
NmuCI GTSAC 7 cut(s) 505, 557, 754, 764, 849, 877, 947
NspI RCATGY 1 cut(s) 103
PaeI GCATGC 1 cut(s) 103
PaeR7I CTCGAG 1 cut(s) 94
PagI TCATGA 1 cut(s) 1114
PasI CCCWGGG 1 cut(s) 315
PfeI GAWTC 2 cut(s) 710, 743
PkrI GCNGC 8 cut(s) 11, 79, 82, 143, 516, 922, 925, 1125
PleI GAGTC 8 cut(s) 269, 292, 390, 412, 430, 445, 573, 874
PmaCI CACGTG 1 cut(s) 159
PmlI CACGTG 1 cut(s) 159
PpsI GAGTC 8 cut(s) 269, 292, 390, 412, 430, 445, 573, 874
Ppu21I YACGTR 1 cut(s) 159
PshAI GACNNNNGTC 1 cut(s) 564
Psp6I CCWGG 1 cut(s) 314
PspCI CACGTG 1 cut(s) 159
PspEI GGTNACC 1 cut(s) 557
PspFI CCCAGC 2 cut(s) 45, 443
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 171, 732
PvuII CAGCTG 1 cut(s) 241
RsaI GTAC 2 cut(s) 1030, 1039
RsaNI GTAC 2 cut(s) 1029, 1038
RseI CAYNNNNRTG 2 cut(s) 863, 954
SacII CCGCGG 1 cut(s) 81
SaqAI TTAA 1 cut(s) 1158
SatI GCNGC 8 cut(s) 10, 78, 81, 142, 515, 921, 924, 1124
Sau3AI GATC 3 cut(s) 197, 940, 1006
ScaI AGTACT 1 cut(s) 1039
SchI GAGTC 8 cut(s) 269, 293, 390, 413, 431, 446, 574, 874
ScrFI CCNGG 3 cut(s) 316, 574, 575
Sfr274I CTCGAG 1 cut(s) 94
Sfr303I CCGCGG 1 cut(s) 81
SgrAI CRCCGGYG 1 cut(s) 628
SgrBI CCGCGG 1 cut(s) 81
SlaI CTCGAG 1 cut(s) 94
SmaI CCCGGG 1 cut(s) 575
SmiMI CAYNNNNRTG 2 cut(s) 863, 954
SmlI CTYRAG 2 cut(s) 94, 774
SmoI CTYRAG 2 cut(s) 94, 774
SphI GCATGC 1 cut(s) 103
Sse9I AATT 2 cut(s) 900, 1056
SsiI CCGC 9 cut(s) 9, 26, 78, 80, 142, 599, 635, 933, 1139
SspMI CTAG 3 cut(s) 138, 207, 548
StyD4I CCNGG 3 cut(s) 314, 572, 573
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 4 cut(s) 110, 855, 947, 1033
TaiI ACGT 4 cut(s) 20, 74, 161, 390
TaqI TCGA 7 cut(s) 41, 95, 196, 584, 863, 891, 1143
TaqII GACCGA 2 cut(s) 260, 736
TasI AATT 2 cut(s) 900, 1056
TatI WGTACW 2 cut(s) 1028, 1037
TauI GCSGC 4 cut(s) 12, 80, 83, 144
TfiI GAWTC 2 cut(s) 710, 743
Tru1I TTAA 1 cut(s) 1158
Tru9I TTAA 1 cut(s) 1158
TscAI CASTG 2 cut(s) 741, 825
TseFI GTSAC 7 cut(s) 505, 557, 754, 764, 849, 877, 947
TseI GCWGC 4 cut(s) 514, 920, 923, 1123
Tsp45I GTSAC 7 cut(s) 505, 557, 754, 764, 849, 877, 947
TspDTI ATGAA 4 cut(s) 390, 984, 1011, 1131
TspGWI ACGGA 1 cut(s) 722
TspMI CCCGGG 1 cut(s) 573
TspRI CASTG 2 cut(s) 741, 825
XapI RAATTY 1 cut(s) 900
XceI RCATGY 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 94
XmaI CCCGGG 1 cut(s) 573
XspI CTAG 3 cut(s) 138, 207, 548
ZraI GACGTC 1 cut(s) 18
ZrmI AGTACT 1 cut(s) 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.