pycom01g22690

Protein SPT2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004232
Physical Location & Seq
Forward (+)
20180662 .. 20182432
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22690.4

Sequence Viewer

Length: 1281 bp
ATGGGAGCACTTTGTGATCCTAGTGAAGCCGAATTGGACTTCCTTCAACAAAGGCTGTTATTCAAAGAACGCTTTCGAACCAATCATATGAAGGAATCGGGGTCTGGTATTGATTGTCCGAACGATAAGAATATAAGTGACAACTTCGGAACATTCTTTGGACCCTCACAACCTACCATATCACGAAGAGTGCTCGAAGAAAGAAAAGTACTAATGCCAGGATTAACGAATTTGGCATCCAAGGTCAGAAATTCTGATGATCAGGGTAAAAAGAGCCTGAAGAGCCCTTCCTCAACTACAACAAGGTCTGGTTCGACAATGAAAAATATAAAGGTATCCCGGGATTACTCCTTTTTGTTGTCAAAGGAAGCAGAACCACCGGCAACTTCAAAAGGGGAGTCTGGACTCGGTAAAGATTCTATCTTGAATTCAAGCAATGCAAGGACTCCGGTTTCTGGTACGAATAGAGCTCCTGTGGCGAAGTTGAAGCCTGAAAAGAAGCAACCTTTGGATGATAAAGAGAGACATCATGAGAAGAGACGATATTTGATGCCAAAGCAGTCTTCCTTGCAACGTAAGGCTCCGAAAGTGAATGAAACTTCTTCAACGAAGTCACTGCCTGCATCGAAGAAACAATTGGAACAACAAAAGGATTTGCATCAACGTACGAAACTAAAACCAAAGCCAGATAAAACTCGGTCTCCTCCACAACAAGAGAATTCAACGAAGTCACTGCCTACATCGAAGAAACAATTGGAGCAACGAAAGTTTTTGCATCAAGCTGCGAAAGCAACACTGAAGCCAGATGATAAAACTCAGTCTCCTCCGCAACGACAGAATCTTGATAGGTGCAAAGCCTCGTCATCAAAGCTACCATCTTCGACAAAGCACGACATTAAAAAAGAAAAGCAAATCCTCCAACCCAATAACAAAAGAGTGATGCCGAAACAGCAAAACTCCTCGAAAATTAAGTTCAGCAGATCAAACGCGATGTGGTGGAGAGTTCTTATGATCACAAAGTTGACGTTTCTTCTATCGATCCAATCAATTTTTAGACGTAAACGTAAGCATATTCGGGAGGAATATTCCGATTCCGACAATGATGACGCTGCAATGGTTTCCACCTTTGACGATATTATTAGAGAAGAGAACAGGAGTACAAAAATTGCTAGGAAGGAGGATGAAATCGAGCGTCTACGGCTCGACGAAGAGAAGCAAGAGAGGTTAAGAAAAGCAAAGAAGCAGAAGCTGAAAGCATACAAAAGCGATTCCAACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPT2

Protein Analysis

427

Amino Acids

48.8

Weight (kDa)

10.12

Isoelectric Point (pI)

62.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1195
AccII CGCG 1 cut(s) 989
AciI CCGC 1 cut(s) 827
AclWI GGATC 2 cut(s) 11, 1033
AcsI RAATTY 4 cut(s) 229, 250, 427, 718
AcuI CTGAAG 2 cut(s) 299, 818
AdeI CACNNNGTG 1 cut(s) 14
AfaI GTAC 4 cut(s) 210, 460, 667, 1159
AfiI CCNNNNNNNGG 1 cut(s) 455
AgsI TTSAA 8 cut(s) 47, 64, 390, 427, 432, 487, 606, 723
AjnI CCWGG 1 cut(s) 217
AjuI GAANNNNNNNTTGG 8 cut(s) 491, 523, 547, 579, 620, 652, 737, 769
AluBI AGCT 4 cut(s) 470, 782, 871, 1249
AluI AGCT 4 cut(s) 470, 782, 871, 1249
Alw21I GWGCWC 3 cut(s) 10, 195, 472
Alw26I GTCTC 4 cut(s) 517, 532, 705, 825
AlwI GGATC 2 cut(s) 11, 1033
AlwNI CAGNNNCTG 1 cut(s) 1249
Ama87I CYCGRG 1 cut(s) 339
ApeKI GCWGC 2 cut(s) 782, 1109
ApoI RAATTY 4 cut(s) 229, 250, 427, 718
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
Asp700I GAANNNNTTC 1 cut(s) 72
AspS9I GGNCC 1 cut(s) 161
AsuC2I CCSGG 2 cut(s) 340, 341
AsuII TTCGAA 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 339
AvaII GGWCC 1 cut(s) 161
BanII GRGCYC 2 cut(s) 287, 472
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 3 cut(s) 10, 195, 472
BbvI GCAGC 2 cut(s) 769, 1096
BccI CCATC 1 cut(s) 883
BceAI ACGGC 1 cut(s) 1214
BcgI CGANNNNNNTGC 4 cut(s) 598, 632, 715, 749
BciT130I CCWGG 1 cut(s) 219
BciVI GTATCC 1 cut(s) 346
BclI TGATCA 2 cut(s) 259, 1011
BcnI CCSGG 2 cut(s) 340, 341
BcoDI GTCTC 4 cut(s) 517, 532, 705, 825
BfaI CTAG 2 cut(s) 21, 1170
BfuI GTATCC 1 cut(s) 346
BisI GCNGC 2 cut(s) 783, 1110
BlsI GCNGC 2 cut(s) 784, 1111
BmcAI AGTACT 1 cut(s) 210
Bme1390I CCNGG 3 cut(s) 219, 340, 341
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 163, 582
BmrFI CCNGG 3 cut(s) 219, 340, 341
BmsI GCATC 6 cut(s) 245, 540, 632, 667, 784, 930
BpiI GAAGAC 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 76
BpuMI CCSGG 2 cut(s) 340, 341
Bsa29I ATCGAT 1 cut(s) 1037
BsaBI GATNNNNATC 1 cut(s) 657
BsaI GGTCTC 1 cut(s) 705
BsaJI CCNNGG 2 cut(s) 240, 339
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 4 cut(s) 685, 715, 805, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse118I RCCGGY 1 cut(s) 379
Bse3DI GCAATG 2 cut(s) 442, 1119
Bse8I GATNNNNATC 1 cut(s) 657
BseBI CCWGG 1 cut(s) 219
BseCI ATCGAT 1 cut(s) 1037
BseDI CCNNGG 2 cut(s) 240, 339
BseGI GGATG 3 cut(s) 236, 517, 1186
BseJI GATNNNNATC 1 cut(s) 657
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMI GCAATG 2 cut(s) 442, 1119
BseMII CTCAG 1 cut(s) 830
BseRI GAGGAG 3 cut(s) 693, 813, 949
BseXI GCAGC 2 cut(s) 769, 1096
Bsh1236I CGCG 1 cut(s) 989
BshVI ATCGAT 1 cut(s) 1037
BsiHKAI GWGCWC 3 cut(s) 10, 195, 472
BsiHKCI CYCGRG 1 cut(s) 339
BsiSI CCGG 3 cut(s) 340, 380, 449
BsiWI CGTACG 1 cut(s) 665
BslI CCNNNNNNNGG 1 cut(s) 455
BsmAI GTCTC 4 cut(s) 517, 532, 705, 825
BsmBI CGTCTC 1 cut(s) 532
Bso31I GGTCTC 1 cut(s) 705
BsoBI CYCGRG 1 cut(s) 339
Bsp119I TTCGAA 1 cut(s) 76
Bsp1286I GDGCHC 4 cut(s) 10, 195, 287, 472
Bsp143I GATC 5 cut(s) 16, 259, 980, 1011, 1038
BspACI CCGC 1 cut(s) 827
BspCNI CTCAG 1 cut(s) 829
BspDI ATCGAT 1 cut(s) 1037
BspFNI CGCG 1 cut(s) 989
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 2 cut(s) 163, 582
BspPI GGATC 2 cut(s) 11, 1033
BspQI GCTCTTC 1 cut(s) 275
BspT104I TTCGAA 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 705
BsrDI GCAATG 2 cut(s) 442, 1119
BsrFI RCCGGY 1 cut(s) 379
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 2 cut(s) 240, 339
BssMI GATC 5 cut(s) 16, 259, 980, 1011, 1038
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 219
Bst6I CTCTTC 5 cut(s) 181, 275, 530, 1140, 1203
BstBI TTCGAA 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 1 cut(s) 816
BstF5I GGATG 3 cut(s) 236, 517, 1186
BstFNI CGCG 1 cut(s) 989
BstKTI GATC 5 cut(s) 19, 262, 983, 1014, 1041
BstMAI GTCTC 4 cut(s) 517, 532, 705, 825
BstMBI GATC 5 cut(s) 16, 259, 980, 1011, 1038
BstMWI GCNNNNNNNGC 5 cut(s) 282, 476, 788, 949, 1198
BstNI CCWGG 1 cut(s) 219
BstSCI CCNGG 3 cut(s) 217, 338, 339
BstUI CGCG 1 cut(s) 989
BstV1I GCAGC 2 cut(s) 769, 1096
BstV2I GAAGAC 1 cut(s) 555
Bsu15I ATCGAT 1 cut(s) 1037
BsuI GTATCC 1 cut(s) 346
BsuTUI ATCGAT 1 cut(s) 1037
BtgZI GCGATG 1 cut(s) 1004
BtsCI GGATG 3 cut(s) 236, 517, 1186
BtsI GCAGTG 2 cut(s) 614, 731
BtsIMutI CAGTG 3 cut(s) 614, 731, 794
Cac8I GCNNGC 1 cut(s) 621
CaiI CAGNNNCTG 1 cut(s) 1249
CciI TCATGA 1 cut(s) 529
Cfr10I RCCGGY 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 161
Cfr9I CCCGGG 1 cut(s) 339
ClaI ATCGAT 1 cut(s) 1037
CseI GACGC 2 cut(s) 1115, 1181
Csp6I GTAC 4 cut(s) 209, 459, 666, 1158
CviAII CATG 1 cut(s) 530
CviQI GTAC 4 cut(s) 209, 459, 666, 1158
DdeI CTNAG 1 cut(s) 816
DpnI GATC 5 cut(s) 18, 261, 982, 1013, 1040
DpnII GATC 5 cut(s) 16, 259, 980, 1011, 1038
DraIII CACNNNGTG 1 cut(s) 14
Eam1104I CTCTTC 5 cut(s) 181, 275, 530, 1140, 1203
EarI CTCTTC 5 cut(s) 181, 275, 530, 1140, 1203
Ecl136II GAGCTC 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 2 cut(s) 287, 472
Eco31I GGTCTC 1 cut(s) 705
Eco47I GGWCC 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 470
Eco57I CTGAAG 2 cut(s) 299, 818
Eco88I CYCGRG 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 470
EcoRI GAATTC 2 cut(s) 427, 718
EcoRII CCWGG 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 240
EcoT38I GRGCYC 2 cut(s) 287, 472
ErhI CCWWGG 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 532
FaeI CATG 1 cut(s) 533
FaiI YATR 9 cut(s) 87, 89, 134, 179, 329, 531, 1010, 1071, 1258
FatI CATG 1 cut(s) 529
FauNDI CATATG 1 cut(s) 87
FbaI TGATCA 2 cut(s) 259, 1011
FblI GTMKAC 1 cut(s) 1195
Fnu4HI GCNGC 2 cut(s) 783, 1110
FokI GGATG 3 cut(s) 223, 524, 1193
FriOI GRGCYC 2 cut(s) 287, 472
Fsp4HI GCNGC 2 cut(s) 783, 1110
FspBI CTAG 2 cut(s) 21, 1170
GluI GCNGC 2 cut(s) 783, 1110
HapII CCGG 3 cut(s) 340, 380, 449
HgaI GACGC 2 cut(s) 1115, 1181
Hin1II CATG 1 cut(s) 533
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HinfI GANTC 8 cut(s) 95, 398, 405, 416, 445, 838, 1091, 1268
HpaII CCGG 3 cut(s) 340, 380, 449
Hpy166II GTNNAC 3 cut(s) 1023, 1061, 1196
Hpy188I TCNGA 7 cut(s) 120, 149, 248, 256, 585, 1090, 1096
Hpy188III TCNNGA 7 cut(s) 183, 402, 424, 530, 842, 1076, 1278
Hpy8I GTNNAC 3 cut(s) 1023, 1061, 1196
Hpy99I CGWCG 1 cut(s) 1208
HpyAV CCTTC 4 cut(s) 53, 85, 297, 1168
HpyCH4IV ACGT 5 cut(s) 574, 664, 1025, 1057, 1063
HpyCH4V TGCA 7 cut(s) 440, 571, 623, 658, 775, 852, 1112
HpyF10VI GCNNNNNNNGC 5 cut(s) 282, 476, 788, 949, 1198
HpyF3I CTNAG 1 cut(s) 816
HpySE526I ACGT 5 cut(s) 574, 664, 1025, 1057, 1063
Hsp92II CATG 1 cut(s) 533
Ksp22I TGATCA 2 cut(s) 259, 1011
Kzo9I GATC 5 cut(s) 16, 259, 980, 1011, 1038
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 4 cut(s) 5, 475, 586, 757
Lsp1109I GCAGC 2 cut(s) 769, 1096
LweI GCATC 6 cut(s) 245, 540, 632, 667, 784, 930
MaeI CTAG 2 cut(s) 21, 1170
MaeII ACGT 5 cut(s) 574, 664, 1025, 1057, 1063
MaeIII GTNAC 3 cut(s) 137, 612, 729
MalI GATC 5 cut(s) 18, 261, 982, 1013, 1040
MboI GATC 5 cut(s) 16, 259, 980, 1011, 1038
MfeI CAATTG 2 cut(s) 635, 752
MhlI GDGCHC 4 cut(s) 10, 195, 287, 472
MlyI GAGTC 3 cut(s) 399, 407, 439
MmeI TCCRAC 2 cut(s) 943, 1119
MroXI GAANNNNTTC 1 cut(s) 72
MseI TTAA 4 cut(s) 224, 897, 969, 1226
MspI CCGG 3 cut(s) 340, 380, 449
MspR9I CCNGG 3 cut(s) 219, 340, 341
MunI CAATTG 2 cut(s) 635, 752
MvaI CCWGG 1 cut(s) 219
MvnI CGCG 1 cut(s) 989
MwoI GCNNNNNNNGC 5 cut(s) 282, 476, 788, 949, 1198
NciI CCSGG 2 cut(s) 340, 341
NdeI CATATG 1 cut(s) 87
NdeII GATC 5 cut(s) 16, 259, 980, 1011, 1038
NlaIII CATG 1 cut(s) 533
NlaIV GGNNCC 2 cut(s) 163, 582
NmuCI GTSAC 3 cut(s) 137, 612, 729
NspV TTCGAA 1 cut(s) 76
PagI TCATGA 1 cut(s) 529
PciSI GCTCTTC 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 72
PfeI GAWTC 5 cut(s) 95, 416, 838, 1091, 1268
Pfl23II CGTACG 1 cut(s) 665
PkrI GCNGC 2 cut(s) 784, 1111
PleI GAGTC 3 cut(s) 399, 406, 439
PpsI GAGTC 3 cut(s) 399, 406, 439
Psp124BI GAGCTC 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 217
PspGI CCWGG 1 cut(s) 217
PspLI CGTACG 1 cut(s) 665
PspN4I GGNNCC 2 cut(s) 163, 582
PspPI GGNCC 1 cut(s) 161
PstNI CAGNNNCTG 1 cut(s) 1249
RsaI GTAC 4 cut(s) 210, 460, 667, 1159
RsaNI GTAC 4 cut(s) 209, 459, 666, 1158
SacI GAGCTC 1 cut(s) 472
SapI GCTCTTC 1 cut(s) 275
SaqAI TTAA 4 cut(s) 224, 897, 969, 1226
SatI GCNGC 2 cut(s) 783, 1110
Sau3AI GATC 5 cut(s) 16, 259, 980, 1011, 1038
Sau96I GGNCC 1 cut(s) 161
ScaI AGTACT 1 cut(s) 210
SchI GAGTC 3 cut(s) 399, 407, 439
ScrFI CCNGG 3 cut(s) 219, 340, 341
SduI GDGCHC 4 cut(s) 10, 195, 287, 472
SfaNI GCATC 6 cut(s) 245, 540, 632, 667, 784, 930
SfuI TTCGAA 1 cut(s) 76
SinI GGWCC 1 cut(s) 161
SmaI CCCGGG 1 cut(s) 341
SsiI CCGC 1 cut(s) 827
SspI AATATT 1 cut(s) 1085
SspMI CTAG 2 cut(s) 21, 1170
SstI GAGCTC 1 cut(s) 472
StyD4I CCNGG 3 cut(s) 217, 338, 339
StyI CCWWGG 1 cut(s) 240
TaiI ACGT 5 cut(s) 577, 667, 1028, 1060, 1066
TaqII GACCGA 1 cut(s) 687
TatI WGTACW 2 cut(s) 208, 1157
TfiI GAWTC 5 cut(s) 95, 416, 838, 1091, 1268
Tru1I TTAA 4 cut(s) 224, 897, 969, 1226
Tru9I TTAA 4 cut(s) 224, 897, 969, 1226
TscAI CASTG 3 cut(s) 621, 738, 801
TseFI GTSAC 3 cut(s) 137, 612, 729
TseI GCWGC 2 cut(s) 782, 1109
Tsp45I GTSAC 3 cut(s) 137, 612, 729
TspDTI ATGAA 4 cut(s) 104, 335, 609, 1197
TspMI CCCGGG 1 cut(s) 339
TspRI CASTG 3 cut(s) 621, 738, 801
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 4 cut(s) 229, 250, 427, 718
XmaI CCCGGG 1 cut(s) 339
XmiI GTMKAC 1 cut(s) 1195
XmnI GAANNNNTTC 1 cut(s) 72
XspI CTAG 2 cut(s) 21, 1170
ZrmI AGTACT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.