Rh3BG063700

Protein SPT2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0004232
Physical Location & Seq
Reverse (-)
4283313 .. 4284608
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG063700.1

Sequence Viewer

Length: 981 bp
ATGTCGATGACGACGATTGGTGTTGCAGGTAACATGGGTATTCCTGAACTCCGGCAAATTCTGAAAGAGCGATACAGAATTGCTAATAACATGAAAAGCTGCTCGGCTGAGATGAAAGAGACCAAGAACTCATCCTTGTTTGGATCCTTGGAGTACTCTCTCTCACAAAGACTGATCGACGAGAGTAAAGCTCTTATGCCGGAGCTGATCAATATTCTGGATAAGATGAACAACGATGATCATCGAAAGCCCACAAAAGCAACAAAGCAAAATTCCGGTGTGGATTTGAAGCTTCAGCGAGCTAAGAAGGTGAAGGAATCGCGTGATTACTCTTTCTTGTTATCAGAGACTTCAGAAGTTCCGAAGATTTCAAAAGCGTCACCACCAATGTCTATTCCTAAAGCTGATGAGAATGGGATCCCAAAGAAGAGTCATGATAAGGCGTCTTCTCTGAAACCAAAGAAGAGTGGTGATGATAACAAGAAGTCCAAAGTCTCCCAGGCAAAGTCAGAGTCCGTTTCGAAATATTCAGTTCTGGTTTCTGGTTCAAAGAGTTTGAAGTCTCGGCCTAGTGAGAACATGGAGACAAAGTCACAATTTTCATCTGCAAAGCAAACCTTGAACCCGAAACAAGCAGAGCTCAGTAACACAAAAGTGATGCCGAAAAAGGAAGGTCTTTGCTTGAAGAAAAAAATGATCAAGAAACGCCGAGTGGAAGAGGATTTGGGGGATGATGGCAATGTAGACTTTTCTTCACTAATCCAAGATATTATGGGGAGAAGAGGAAAGCGGCGATACAGGGATTATGGAGACGACGGCGATGATCGTGCTATGGTGTCTGGCTTTAATGATATTCTGAAAGAAGAGAGGAGGAGTGCAGAGATTGCGAGGAAGGAGGATGCAATAGAGGCTCTAAGGCTTGAGCAAGAAGCCAAAGAAGAGAGGTTGAGCAAAGCAAAGAAGCAGAAGTTGAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPT2

Protein Analysis

326

Amino Acids

36.7

Weight (kDa)

9.7

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT2 PF08243 238 - 318 7.1e-12 SPT2 chromatin protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 744
AccII CGCG 1 cut(s) 322
AciI CCGC 1 cut(s) 790
AclWI GGATC 4 cut(s) 138, 151, 412, 425
AcsI RAATTY 2 cut(s) 57, 271
AcuI CTGAAG 2 cut(s) 278, 336
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 1 cut(s) 155
AgsI TTSAA 7 cut(s) 289, 372, 549, 559, 622, 685, 973
AjnI CCWGG 1 cut(s) 498
AleI CACNNNNGTG 1 cut(s) 653
AluBI AGCT 7 cut(s) 99, 191, 205, 292, 302, 404, 640
AluI AGCT 7 cut(s) 99, 191, 205, 292, 302, 404, 640
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 6 cut(s) 113, 341, 499, 567, 578, 804
AlwI GGATC 4 cut(s) 138, 151, 412, 425
AoxI GGCC 1 cut(s) 566
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 2 cut(s) 57, 271
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
AsuHPI GGTGA 3 cut(s) 322, 372, 482
AsuII TTCGAA 1 cut(s) 521
BamHI GGATCC 2 cut(s) 143, 417
BanII GRGCYC 1 cut(s) 642
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 1 cut(s) 86
BccI CCATC 1 cut(s) 728
BceAI ACGGC 1 cut(s) 832
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 1 cut(s) 500
BclI TGATCA 3 cut(s) 207, 238, 696
BcoDI GTCTC 6 cut(s) 113, 341, 499, 567, 578, 804
BfaI CTAG 1 cut(s) 570
BfuAI ACCTGC 1 cut(s) 17
BisI GCNGC 2 cut(s) 100, 791
BlsI GCNGC 2 cut(s) 101, 792
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 1 cut(s) 500
BmiI GGNNCC 2 cut(s) 145, 419
BmrFI CCNGG 1 cut(s) 500
BmsI GCATC 2 cut(s) 648, 889
BpiI GAAGAC 1 cut(s) 438
BplI GAGNNNNNCTC 2 cut(s) 175, 207
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 1 cut(s) 941
BsaBI GATNNNNATC 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 147, 498
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
Bse3DI GCAATG 1 cut(s) 745
Bse8I GATNNNNATC 1 cut(s) 240
BseBI CCWGG 1 cut(s) 500
BseDI CCNNGG 2 cut(s) 147, 498
BseGI GGATG 3 cut(s) 131, 736, 904
BseJI GATNNNNATC 1 cut(s) 240
BseMI GCAATG 1 cut(s) 745
BseMII CTCAG 2 cut(s) 99, 655
BseRI GAGGAG 2 cut(s) 883, 886
BseXI GCAGC 1 cut(s) 86
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 3 cut(s) 52, 200, 276
BsmAI GTCTC 6 cut(s) 113, 341, 499, 567, 578, 804
BsmBI CGTCTC 1 cut(s) 804
BsnI GGCC 1 cut(s) 568
Bso31I GGTCTC 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 7 cut(s) 143, 174, 207, 238, 417, 696, 823
BspACI CCGC 1 cut(s) 790
BspANI GGCC 1 cut(s) 568
BspCNI CTCAG 2 cut(s) 100, 654
BspFNI CGCG 1 cut(s) 322
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 2 cut(s) 145, 419
BspMI ACCTGC 1 cut(s) 17
BspPI GGATC 4 cut(s) 138, 151, 412, 425
BspT104I TTCGAA 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 745
BssECI CCNNGG 2 cut(s) 147, 498
BssMI GATC 7 cut(s) 143, 174, 207, 238, 417, 696, 823
BssNI GRCGYC 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 500
Bst6I CTCTTC 6 cut(s) 422, 458, 711, 775, 858, 933
BstACI GRCGYC 1 cut(s) 443
BstAPI GCANNNNNTGC 1 cut(s) 884
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 4 cut(s) 108, 303, 641, 914
BstF5I GGATG 3 cut(s) 131, 736, 904
BstFNI CGCG 1 cut(s) 322
BstKTI GATC 7 cut(s) 146, 177, 210, 241, 420, 699, 826
BstMAI GTCTC 6 cut(s) 113, 341, 499, 567, 578, 804
BstMBI GATC 7 cut(s) 143, 174, 207, 238, 417, 696, 823
BstMWI GCNNNNNNNGC 2 cut(s) 884, 908
BstNI CCWGG 1 cut(s) 500
BstSCI CCNGG 1 cut(s) 498
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 86
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 143, 417
BstYI RGATCY 2 cut(s) 143, 417
BsuRI GGCC 1 cut(s) 568
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 3 cut(s) 131, 736, 904
BveI ACCTGC 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 300
CciI TCATGA 1 cut(s) 433
CseI GACGC 2 cut(s) 366, 432
Csp6I GTAC 1 cut(s) 154
CviAII CATG 4 cut(s) 34, 91, 434, 580
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 4 cut(s) 108, 303, 641, 914
DpnI GATC 7 cut(s) 145, 176, 209, 240, 419, 698, 825
DpnII GATC 7 cut(s) 143, 174, 207, 238, 417, 696, 823
Eam1104I CTCTTC 6 cut(s) 422, 458, 711, 775, 858, 933
EarI CTCTTC 6 cut(s) 422, 458, 711, 775, 858, 933
Ecl136II GAGCTC 1 cut(s) 640
Eco130I CCWWGG 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 640
Eco57I CTGAAG 2 cut(s) 278, 336
EcoICRI GAGCTC 1 cut(s) 640
EcoRII CCWGG 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 147
EcoT38I GRGCYC 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 804
FaeI CATG 4 cut(s) 37, 94, 437, 583
FaiI YATR 8 cut(s) 35, 92, 197, 435, 581, 773, 807, 833
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FatI CATG 4 cut(s) 33, 90, 433, 579
FbaI TGATCA 3 cut(s) 207, 238, 696
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 2 cut(s) 100, 791
FokI GGATG 3 cut(s) 118, 743, 911
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 2 cut(s) 100, 791
FspBI CTAG 1 cut(s) 570
GluI GCNGC 2 cut(s) 100, 791
HaeIII GGCC 1 cut(s) 568
HapII CCGG 3 cut(s) 52, 200, 276
HgaI GACGC 2 cut(s) 366, 432
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 4 cut(s) 37, 94, 437, 583
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 3 cut(s) 317, 430, 512
HpaII CCGG 3 cut(s) 52, 200, 276
HphI GGTGA 3 cut(s) 322, 372, 482
Hpy166II GTNNAC 1 cut(s) 745
Hpy188I TCNGA 7 cut(s) 63, 346, 355, 363, 453, 511, 858
Hpy188III TCNNGA 4 cut(s) 44, 218, 434, 700
Hpy8I GTNNAC 1 cut(s) 745
Hpy99I CGWCG 3 cut(s) 16, 182, 818
HpyAV CCTTC 4 cut(s) 301, 307, 665, 886
HpyCH4V TGCA 4 cut(s) 26, 608, 878, 902
HpyF10VI GCNNNNNNNGC 2 cut(s) 884, 908
HpyF3I CTNAG 4 cut(s) 108, 303, 641, 914
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 4 cut(s) 37, 94, 437, 583
Ksp22I TGATCA 3 cut(s) 207, 238, 696
Kzo9I GATC 7 cut(s) 143, 174, 207, 238, 417, 696, 823
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 2 cut(s) 648, 889
MaeI CTAG 1 cut(s) 570
MaeIII GTNAC 4 cut(s) 29, 378, 591, 644
MalI GATC 7 cut(s) 145, 176, 209, 240, 419, 698, 825
MboI GATC 7 cut(s) 143, 174, 207, 238, 417, 696, 823
MflI RGATCY 2 cut(s) 143, 417
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 5 cut(s) 57, 78, 271, 596, 976
MlyI GAGTC 2 cut(s) 439, 521
MnlI CCTC 8 cut(s) 712, 776, 861, 864, 882, 889, 901, 936
MseI TTAA 1 cut(s) 846
MslI CAYNNNNRTG 1 cut(s) 653
MspI CCGG 3 cut(s) 52, 200, 276
MspR9I CCNGG 1 cut(s) 500
MvaI CCWGG 1 cut(s) 500
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 884, 908
NdeII GATC 7 cut(s) 143, 174, 207, 238, 417, 696, 823
NlaIII CATG 4 cut(s) 37, 94, 437, 583
NlaIV GGNNCC 2 cut(s) 145, 419
NmeAIII GCCGAG 3 cut(s) 83, 544, 734
NmuCI GTSAC 2 cut(s) 378, 591
NspV TTCGAA 1 cut(s) 521
OliI CACNNNNGTG 1 cut(s) 653
PagI TCATGA 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PfeI GAWTC 1 cut(s) 317
PflFI GACNNNGTC 1 cut(s) 589
PkrI GCNGC 2 cut(s) 101, 792
PleI GAGTC 2 cut(s) 438, 520
PpsI GAGTC 2 cut(s) 438, 520
Psp124BI GAGCTC 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 498
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 145, 419
PsuI RGATCY 2 cut(s) 143, 417
PsyI GACNNNGTC 1 cut(s) 589
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 653
SacI GAGCTC 1 cut(s) 642
SaqAI TTAA 1 cut(s) 846
SatI GCNGC 2 cut(s) 100, 791
Sau3AI GATC 7 cut(s) 143, 174, 207, 238, 417, 696, 823
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 2 cut(s) 439, 521
ScrFI CCNGG 1 cut(s) 500
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 2 cut(s) 648, 889
SfuI TTCGAA 1 cut(s) 521
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
Sse9I AATT 5 cut(s) 57, 78, 271, 596, 976
SsiI CCGC 1 cut(s) 790
SspI AATATT 2 cut(s) 214, 527
SspMI CTAG 1 cut(s) 570
SstI GAGCTC 1 cut(s) 642
StyD4I CCNGG 1 cut(s) 498
StyI CCWWGG 1 cut(s) 147
TaqI TCGA 4 cut(s) 5, 177, 244, 521
TasI AATT 5 cut(s) 57, 78, 271, 596, 976
TatI WGTACW 1 cut(s) 153
TauI GCSGC 1 cut(s) 793
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 1 cut(s) 846
Tru9I TTAA 1 cut(s) 846
TseFI GTSAC 2 cut(s) 378, 591
TseI GCWGC 1 cut(s) 99
Tsp45I GTSAC 2 cut(s) 378, 591
TspDTI ATGAA 4 cut(s) 107, 128, 242, 591
TspGWI ACGGA 1 cut(s) 505
Tth111I GACNNNGTC 1 cut(s) 589
XapI RAATTY 2 cut(s) 57, 271
XmiI GTMKAC 1 cut(s) 744
XspI CTAG 1 cut(s) 570
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.