Rh3BG063300

Protein SPT2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0004232
Physical Location & Seq
Reverse (-)
4244211 .. 4245506
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG063300.1

Sequence Viewer

Length: 981 bp
ATGTCGATGACGACGATTGGTGTTGCAGGTAACATGGGTATTCCTGAACTCCGGCAAATTCTGAAAGAGCGATACAGAATCGCTAATAACGTGAAAAGCTGCTCGGCTGAGATGAAAGAGACCAAGAACTCATCCTTGTTTGGGTCCTCGGAGTACTCTCTTTCACAAAGACTGATCGACGAGAGTAAAGCTCTTATGCCGGAGATGATCAATATTCTGGATAAGATGAACAACAATGATCATCAAAAGCCCACAAAAGCAACAAAGCAAAATTCCGGTGTGGATTTGAAGCTTGAGCGAGCTAAGAAGGTGAAGGAATCGCGTGATTACTCTTTCTTGTTATCAGAGACTTCAGAAGTTCCGAAGATTTCAAAAGCGTCACCACCAATGTCCATTCCTAAACCTGATGGTAATGGGATCCCAAAGAAGAGTCATGATAAGGCGTCTTCTCTGAAACCAAAGAAAAGTGGTGATGATAACAACAAGTCCAAAGTCTCCTCGGCAAAGTCAGAGTCTGTTTCGAAAGATTCAGTTCTGGTTTCTGGTCCAGAGAGTTTTAAGTCTCGGCCTCGTGAGAACATGGTGACAAAGTCACAAGTTTCATCTACAAAGCAAACCTTGAACCCGAAACAAGCAGAGCTCTGTAACACAAAAGTGATGCCGAAAAAGGAAGGTCCTTGCCTGAAGAAAAAAATGATCAAGAAACGCCGAGTCGAAGAGGACTTGGGGGATGATGGCAATGTAGACTTTTCTTCACTAATCCAAGATATTATGGGGAGAAGAGGAAAGAGGCGATATAGGAATTATGGTGACGGCGGCGATGATCATGCTATGGTGTCTGGCTTTAATGATATTCTGAAAGAAGAGAGGAGGAGTGCAGAGATTGCGAGGAAGGAGGATGCAATAGAGGCTCTAAGGCTTGAGCAAGAAGCCAAAGAAGAGAGGTTGAGCAAAGCAAAGAAGCAGAGGTTGGAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPT2

Protein Analysis

326

Amino Acids

36.5

Weight (kDa)

9.61

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT2 PF08243 239 - 318 5.5e-12 SPT2 chromatin protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 744
AccII CGCG 1 cut(s) 322
AciI CCGC 1 cut(s) 816
AclWI GGATC 2 cut(s) 412, 425
AcsI RAATTY 2 cut(s) 57, 271
AcuI CTGAAG 2 cut(s) 336, 704
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 141
AgsI TTSAA 3 cut(s) 289, 372, 622
AjuI GAANNNNNNNTTGG 1 cut(s) 953
AleI CACNNNNGTG 1 cut(s) 653
AluBI AGCT 5 cut(s) 99, 191, 292, 302, 640
AluI AGCT 5 cut(s) 99, 191, 292, 302, 640
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 4 cut(s) 113, 341, 499, 567
AlwI GGATC 2 cut(s) 412, 425
AlwNI CAGNNNCTG 1 cut(s) 515
AoxI GGCC 1 cut(s) 566
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 2 cut(s) 57, 271
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
AspS9I GGNCC 3 cut(s) 144, 545, 674
AsuHPI GGTGA 5 cut(s) 322, 372, 482, 595, 821
AsuII TTCGAA 1 cut(s) 521
AvaII GGWCC 3 cut(s) 144, 545, 674
BamHI GGATCC 1 cut(s) 417
BanII GRGCYC 1 cut(s) 642
BauI CACGAG 1 cut(s) 570
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 1 cut(s) 86
BccI CCATC 2 cut(s) 401, 728
BceAI ACGGC 1 cut(s) 829
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BclI TGATCA 4 cut(s) 207, 238, 696, 823
BcoDI GTCTC 4 cut(s) 113, 341, 499, 567
BfuAI ACCTGC 1 cut(s) 17
BisI GCNGC 2 cut(s) 100, 817
BlsI GCNGC 2 cut(s) 101, 818
BmcAI AGTACT 1 cut(s) 155
Bme18I GGWCC 3 cut(s) 144, 545, 674
BmgT120I GGNCC 3 cut(s) 144, 545, 674
BmiI GGNNCC 2 cut(s) 145, 419
BmsI GCATC 2 cut(s) 648, 889
BpiI GAAGAC 1 cut(s) 438
BplI GAGNNNNNCTC 2 cut(s) 175, 207
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 2 cut(s) 314, 941
BsaHI GRCGYC 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 147, 498
BsaWI WCCGGW 1 cut(s) 275
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 745
BseDI CCNNGG 2 cut(s) 147, 498
BseGI GGATG 3 cut(s) 131, 736, 904
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMI GCAATG 1 cut(s) 745
BseMII CTCAG 1 cut(s) 99
BseRI GAGGAG 3 cut(s) 487, 883, 886
BseXI GCAGC 1 cut(s) 86
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 3 cut(s) 52, 200, 276
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 4 cut(s) 113, 341, 499, 567
BsnI GGCC 1 cut(s) 568
Bso31I GGTCTC 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 6 cut(s) 174, 207, 238, 417, 696, 823
BspACI CCGC 1 cut(s) 816
BspANI GGCC 1 cut(s) 568
BspCNI CTCAG 1 cut(s) 100
BspFNI CGCG 1 cut(s) 322
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 2 cut(s) 145, 419
BspMI ACCTGC 1 cut(s) 17
BspPI GGATC 2 cut(s) 412, 425
BspT104I TTCGAA 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 745
BssECI CCNNGG 2 cut(s) 147, 498
BssMI GATC 6 cut(s) 174, 207, 238, 417, 696, 823
BssNI GRCGYC 1 cut(s) 443
BssSI CACGAG 1 cut(s) 570
Bst2BI CACGAG 1 cut(s) 570
Bst6I CTCTTC 5 cut(s) 422, 711, 775, 858, 933
BstACI GRCGYC 1 cut(s) 443
BstAPI GCANNNNNTGC 1 cut(s) 884
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 3 cut(s) 108, 303, 914
BstF5I GGATG 3 cut(s) 131, 736, 904
BstFNI CGCG 1 cut(s) 322
BstKTI GATC 6 cut(s) 177, 210, 241, 420, 699, 826
BstMAI GTCTC 4 cut(s) 113, 341, 499, 567
BstMBI GATC 6 cut(s) 174, 207, 238, 417, 696, 823
BstMWI GCNNNNNNNGC 2 cut(s) 884, 908
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 86
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
BsuRI GGCC 1 cut(s) 568
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 3 cut(s) 131, 736, 904
BveI ACCTGC 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 300
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 433
Cfr13I GGNCC 3 cut(s) 144, 545, 674
CseI GACGC 2 cut(s) 366, 432
Csp6I GTAC 1 cut(s) 154
CviAII CATG 4 cut(s) 34, 434, 580, 827
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 3 cut(s) 108, 303, 914
DpnI GATC 6 cut(s) 176, 209, 240, 419, 698, 825
DpnII GATC 6 cut(s) 174, 207, 238, 417, 696, 823
Eam1104I CTCTTC 5 cut(s) 422, 711, 775, 858, 933
EarI CTCTTC 5 cut(s) 422, 711, 775, 858, 933
Ecl136II GAGCTC 1 cut(s) 640
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 3 cut(s) 144, 545, 674
Eco53kI GAGCTC 1 cut(s) 640
Eco57I CTGAAG 2 cut(s) 336, 704
EcoICRI GAGCTC 1 cut(s) 640
EcoO109I RGGNCCY 2 cut(s) 144, 674
EcoT38I GRGCYC 1 cut(s) 642
FaeI CATG 4 cut(s) 37, 437, 583, 830
FaiI YATR 9 cut(s) 35, 197, 435, 581, 773, 798, 807, 828, 833
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FatI CATG 4 cut(s) 33, 433, 579, 826
FbaI TGATCA 4 cut(s) 207, 238, 696, 823
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 2 cut(s) 100, 817
FokI GGATG 3 cut(s) 118, 743, 911
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 2 cut(s) 100, 817
GluI GCNGC 2 cut(s) 100, 817
HaeIII GGCC 1 cut(s) 568
HapII CCGG 3 cut(s) 52, 200, 276
HgaI GACGC 2 cut(s) 366, 432
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 4 cut(s) 37, 437, 583, 830
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 6 cut(s) 78, 317, 430, 512, 527, 711
HpaII CCGG 3 cut(s) 52, 200, 276
HphI GGTGA 5 cut(s) 322, 372, 482, 595, 821
Hpy166II GTNNAC 1 cut(s) 745
Hpy188I TCNGA 8 cut(s) 63, 151, 346, 355, 363, 453, 511, 858
Hpy188III TCNNGA 6 cut(s) 44, 218, 434, 548, 572, 700
Hpy8I GTNNAC 1 cut(s) 745
Hpy99I CGWCG 2 cut(s) 16, 182
HpyAV CCTTC 4 cut(s) 301, 307, 665, 886
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 3 cut(s) 26, 878, 902
HpyF10VI GCNNNNNNNGC 2 cut(s) 884, 908
HpyF3I CTNAG 3 cut(s) 108, 303, 914
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 4 cut(s) 37, 437, 583, 830
Ksp22I TGATCA 4 cut(s) 207, 238, 696, 823
Kzo9I GATC 6 cut(s) 174, 207, 238, 417, 696, 823
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 2 cut(s) 648, 889
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 6 cut(s) 29, 378, 583, 591, 644, 809
MalI GATC 6 cut(s) 176, 209, 240, 419, 698, 825
MboI GATC 6 cut(s) 174, 207, 238, 417, 696, 823
MboII GAAGA 9 cut(s) 376, 438, 439, 697, 728, 744, 792, 875, 950
MflI RGATCY 1 cut(s) 417
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 4 cut(s) 57, 271, 802, 976
MlyI GAGTC 3 cut(s) 439, 521, 720
MmeI TCCRAC 1 cut(s) 951
MseI TTAA 2 cut(s) 558, 846
MslI CAYNNNNRTG 1 cut(s) 653
MspI CCGG 3 cut(s) 52, 200, 276
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 884, 908
NdeII GATC 6 cut(s) 174, 207, 238, 417, 696, 823
NlaIII CATG 4 cut(s) 37, 437, 583, 830
NlaIV GGNNCC 2 cut(s) 145, 419
NmeAIII GCCGAG 4 cut(s) 83, 479, 544, 734
NmuCI GTSAC 4 cut(s) 378, 583, 591, 809
NspV TTCGAA 1 cut(s) 521
OliI CACNNNNGTG 1 cut(s) 653
PagI TCATGA 1 cut(s) 433
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 87
PfeI GAWTC 3 cut(s) 78, 317, 527
PflFI GACNNNGTC 1 cut(s) 589
PkrI GCNGC 2 cut(s) 101, 818
PleI GAGTC 3 cut(s) 438, 520, 719
PpsI GAGTC 3 cut(s) 438, 520, 719
PpuMI RGGWCCY 2 cut(s) 144, 674
Psp124BI GAGCTC 1 cut(s) 642
Psp5II RGGWCCY 2 cut(s) 144, 674
PspN4I GGNNCC 2 cut(s) 145, 419
PspPI GGNCC 3 cut(s) 144, 545, 674
PspPPI RGGWCCY 2 cut(s) 144, 674
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 1 cut(s) 417
PsyI GACNNNGTC 1 cut(s) 589
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 653
SacI GAGCTC 1 cut(s) 642
SaqAI TTAA 2 cut(s) 558, 846
SatI GCNGC 2 cut(s) 100, 817
Sau3AI GATC 6 cut(s) 174, 207, 238, 417, 696, 823
Sau96I GGNCC 3 cut(s) 144, 545, 674
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 3 cut(s) 439, 521, 720
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 2 cut(s) 648, 889
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 3 cut(s) 144, 545, 674
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 2 cut(s) 293, 920
SmoI CTYRAG 2 cut(s) 293, 920
Sse9I AATT 4 cut(s) 57, 271, 802, 976
SsiI CCGC 1 cut(s) 816
SspI AATATT 1 cut(s) 214
SstI GAGCTC 1 cut(s) 642
TaiI ACGT 1 cut(s) 93
TaqI TCGA 4 cut(s) 5, 177, 521, 714
TasI AATT 4 cut(s) 57, 271, 802, 976
TatI WGTACW 1 cut(s) 153
TauI GCSGC 1 cut(s) 819
TfiI GAWTC 3 cut(s) 78, 317, 527
Tru1I TTAA 2 cut(s) 558, 846
Tru9I TTAA 2 cut(s) 558, 846
TseFI GTSAC 4 cut(s) 378, 583, 591, 809
TseI GCWGC 1 cut(s) 99
Tsp45I GTSAC 4 cut(s) 378, 583, 591, 809
TspDTI ATGAA 3 cut(s) 128, 242, 591
Tth111I GACNNNGTC 1 cut(s) 589
VpaK11BI GGWCC 3 cut(s) 144, 545, 674
XapI RAATTY 2 cut(s) 57, 271
XmiI GTMKAC 1 cut(s) 744
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.