pycom05g13400

Protein SPT2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004232
Physical Location & Seq
Forward (+)
16959436 .. 16960410
975 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13400.1

Sequence Viewer

Length: 975 bp
ATGGCTGCTCTAGTTACGCGTCCTGCCAAGACGGAGCAATTCGACTTCCTTCAACTCAGGCAGCAACTGAAAGATCGCATTCGCATGAAGAAATCAGGAGGCTCTGCTGCTGCTCAAGGCTGCTCGGTTAATAGTACTACCGAAAACTTCGGATCCTTTTTCGGTCCCTCAGAGCGTGTCATGGCCAAGAGAGTGCACGAAGAGATGAAGGATTTTGTGCCGGAGTTGAAGAAGTTGGCGTCTAGGGTTCTGAATTCCGGTGACAAGGCTAAAAGCACCGCAAAGAGTCCTGCACCCTTGAGCAAGGCATTGAAGTGTAAAATCATCAAGGAGTCCCGGGATTATTCCTTTTTGCAATCCGACAGTGCAGAAATTCCAAAGCCATCTAAAGGAGAGCCTGAAGAAGTTGGCAAGAGCAAAACCTCCTCGGCAAAATCTGAATCCTCTTCAACAAAGCAATGCAGCACAAAACCAATGCCGAAACAAAAGAAATCCGTCTCGAAACATCCAATCAAATATCACGTGAAGGAGGAGCGTCCGGAGGCTGTGATTGGCAAAGCCTACTTGGCAAAGTCAGAATCTTGTTCAACAAAGCAACGCTTGAAGCAAATGGAGCAATCTATTGAGCTGAAACGCACAGCAAAACCGATACCAAAACCGAAACTATCCGTCTCGAAGAATCAGGTCAAAGACCGTGTCAAGGAGGAGCAATCACTTGATGTTTCTTCTGTGATCCAGGCGATATTTGGGCATAACCGCAAGCGCCCTAGGGCGGATGATGAGGATGATGATGGAGATGATCGTGCGATGGTGTCCAGTTTTGATGACATTCTGAAGGAAGAGAGGAAGAGTGCAAAAATCGCTAGGGAGGAAGATGAAAGAGAGCGGTTGCTGCTCGAACAAGAGGAGAAGCAAGAAGAAGGATTAAGAGCAACAAAGAAGCTGAAGATTAAGATCAAATTATCCAAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPT2

Protein Analysis

325

Amino Acids

36.44

Weight (kDa)

9.66

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT2 PF08243 229 - 307 4e-14 SPT2 chromatin protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 886
AccII CGCG 1 cut(s) 19
AccIII TCCGGA 1 cut(s) 538
AciI CCGC 4 cut(s) 279, 757, 773, 886
AclWI GGATC 3 cut(s) 147, 160, 727
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 2 cut(s) 253, 372
AcuI CTGAAG 3 cut(s) 420, 854, 965
AcvI CACGTG 1 cut(s) 523
AcyI GRCGYC 1 cut(s) 239
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 3 cut(s) 389, 700, 772
AflIII ACRYGT 1 cut(s) 17
AgsI TTSAA 6 cut(s) 53, 229, 313, 450, 588, 604
AjnI CCWGG 1 cut(s) 735
AluBI AGCT 2 cut(s) 628, 943
AluI AGCT 2 cut(s) 628, 943
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 2 cut(s) 502, 676
Alw44I GTGCAC 1 cut(s) 194
AlwI GGATC 3 cut(s) 147, 160, 727
AlwNI CAGNNNCTG 1 cut(s) 67
Ama87I CYCGRG 1 cut(s) 336
Aor13HI TCCGGA 1 cut(s) 538
AoxI GGCC 1 cut(s) 183
ApaLI GTGCAC 1 cut(s) 194
ApeKI GCWGC 7 cut(s) 5, 61, 107, 110, 120, 462, 892
ApoI RAATTY 2 cut(s) 253, 372
ArsI GACNNNNNNTTYG 2 cut(s) 681, 713
AspA2I CCTAGG 1 cut(s) 767
AspLEI GCGC 1 cut(s) 765
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 2 cut(s) 337, 338
AsuHPI GGTGA 1 cut(s) 272
AvaI CYCGRG 1 cut(s) 336
AvaII GGWCC 1 cut(s) 164
AvrII CCTAGG 1 cut(s) 767
BaeGI GKGCMC 1 cut(s) 198
BalI TGGCCA 1 cut(s) 185
BamHI GGATCC 1 cut(s) 152
BbrPI CACGTG 1 cut(s) 523
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 6 cut(s) 73, 94, 97, 107, 474, 879
BccI CCATC 3 cut(s) 391, 785, 802
BciT130I CCWGG 1 cut(s) 737
BcnI CCSGG 2 cut(s) 337, 338
BcoDI GTCTC 2 cut(s) 502, 676
BfaI CTAG 4 cut(s) 11, 243, 768, 864
BfoI RGCGCY 1 cut(s) 766
BglI GCCNNNNNGGC 1 cut(s) 566
BisI GCNGC 7 cut(s) 6, 62, 108, 111, 121, 463, 893
BlnI CCTAGG 1 cut(s) 767
BlsI GCNGC 7 cut(s) 7, 63, 109, 112, 122, 464, 894
BmcAI AGTACT 1 cut(s) 136
Bme1390I CCNGG 3 cut(s) 337, 338, 737
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 336
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 154, 166
BmrFI CCNGG 3 cut(s) 337, 338, 737
BpuEI CTTGAG 2 cut(s) 99, 319
BpuMI CCSGG 2 cut(s) 337, 338
BsaAI YACGTR 1 cut(s) 523
BsaBI GATNNNNATC 1 cut(s) 953
BsaHI GRCGYC 1 cut(s) 239
BsaJI CCNNGG 3 cut(s) 336, 426, 767
BsaWI WCCGGW 2 cut(s) 257, 538
Bsc4I CCNNNNNNNGG 3 cut(s) 389, 700, 772
Bse1I ACTGG 1 cut(s) 816
Bse3DI GCAATG 1 cut(s) 464
Bse8I GATNNNNATC 1 cut(s) 953
BseAI TCCGGA 1 cut(s) 538
BseBI CCWGG 1 cut(s) 737
BseDI CCNNGG 3 cut(s) 336, 426, 767
BseGI GGATG 3 cut(s) 505, 781, 790
BseJI GATNNNNATC 1 cut(s) 953
BseLI CCNNNNNNNGG 3 cut(s) 389, 700, 772
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 2 cut(s) 70, 183
BseNI ACTGG 1 cut(s) 816
BseRI GAGGAG 4 cut(s) 415, 545, 719, 920
BseSI GKGCMC 1 cut(s) 198
BseXI GCAGC 6 cut(s) 73, 94, 97, 107, 474, 879
BsgI GTGCAG 2 cut(s) 276, 387
Bsh1236I CGCG 1 cut(s) 19
BshFI GGCC 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 336
BsiSI CCGG 4 cut(s) 221, 258, 337, 539
BslFI GGGAC 2 cut(s) 150, 319
BslI CCNNNNNNNGG 3 cut(s) 389, 700, 772
BsmAI GTCTC 2 cut(s) 502, 676
BsmBI CGTCTC 2 cut(s) 502, 676
BsmFI GGGAC 2 cut(s) 150, 319
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 198
Bsp13I TCCGGA 1 cut(s) 538
Bsp143I GATC 5 cut(s) 73, 152, 732, 799, 954
BspACI CCGC 4 cut(s) 279, 757, 773, 886
BspANI GGCC 1 cut(s) 185
BspCNI CTCAG 2 cut(s) 69, 182
BspEI TCCGGA 1 cut(s) 538
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 154, 166
BspPI GGATC 3 cut(s) 147, 160, 727
BsrBI CCGCTC 1 cut(s) 886
BsrDI GCAATG 1 cut(s) 464
BsrI ACTGG 1 cut(s) 816
BssECI CCNNGG 3 cut(s) 336, 426, 767
BssMI GATC 5 cut(s) 73, 152, 732, 799, 954
BssNI GRCGYC 1 cut(s) 239
BssT1I CCWWGG 1 cut(s) 767
Bst2UI CCWGG 1 cut(s) 737
Bst4CI ACNGT 2 cut(s) 365, 695
Bst6I CTCTTC 4 cut(s) 195, 451, 834, 842
BstACI GRCGYC 1 cut(s) 239
BstBAI YACGTR 1 cut(s) 523
BstC8I GCNNGC 1 cut(s) 761
BstDEI CTNAG 2 cut(s) 56, 169
BstF5I GGATG 3 cut(s) 505, 781, 790
BstFNI CGCG 1 cut(s) 19
BstH2I RGCGCY 1 cut(s) 766
BstHHI GCGC 1 cut(s) 765
BstKTI GATC 5 cut(s) 76, 155, 735, 802, 957
BstMAI GTCTC 2 cut(s) 502, 676
BstMBI GATC 5 cut(s) 73, 152, 732, 799, 954
BstMWI GCNNNNNNNGC 4 cut(s) 566, 613, 860, 892
BstNI CCWGG 1 cut(s) 737
BstSCI CCNGG 3 cut(s) 335, 336, 735
BstSLI GKGCMC 1 cut(s) 198
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 6 cut(s) 73, 94, 97, 107, 474, 879
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BsuRI GGCC 1 cut(s) 185
BtgZI GCGATG 1 cut(s) 821
BtsCI GGATG 3 cut(s) 505, 781, 790
BtsIMutI CAGTG 1 cut(s) 370
Cac8I GCNNGC 1 cut(s) 761
CaiI CAGNNNCTG 1 cut(s) 67
CfoI GCGC 1 cut(s) 765
Cfr13I GGNCC 1 cut(s) 164
Cfr9I CCCGGG 1 cut(s) 336
CseI GACGC 3 cut(s) 8, 228, 524
Csp6I GTAC 1 cut(s) 135
CviAII CATG 3 cut(s) 85, 181, 972
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 2 cut(s) 56, 169
DpnI GATC 5 cut(s) 75, 154, 734, 801, 956
DpnII GATC 5 cut(s) 73, 152, 732, 799, 954
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 4 cut(s) 195, 451, 834, 842
EarI CTCTTC 4 cut(s) 195, 451, 834, 842
EciI GGCGGA 1 cut(s) 788
Eco130I CCWWGG 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 164
Eco57I CTGAAG 3 cut(s) 420, 854, 965
Eco72I CACGTG 1 cut(s) 523
Eco88I CYCGRG 1 cut(s) 336
EcoRI GAATTC 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 735
EcoT14I CCWWGG 1 cut(s) 767
ErhI CCWWGG 1 cut(s) 767
Esp3I CGTCTC 2 cut(s) 502, 676
FaeI CATG 3 cut(s) 88, 184, 975
FaiI YATR 4 cut(s) 86, 182, 753, 973
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FaqI GGGAC 2 cut(s) 150, 319
FatI CATG 3 cut(s) 84, 180, 971
Fnu4HI GCNGC 7 cut(s) 6, 62, 108, 111, 121, 463, 893
FokI GGATG 3 cut(s) 492, 788, 797
Fsp4HI GCNGC 7 cut(s) 6, 62, 108, 111, 121, 463, 893
FspBI CTAG 4 cut(s) 11, 243, 768, 864
GlaI GCGC 1 cut(s) 764
GluI GCNGC 7 cut(s) 6, 62, 108, 111, 121, 463, 893
HaeII RGCGCY 1 cut(s) 766
HaeIII GGCC 1 cut(s) 185
HapII CCGG 4 cut(s) 221, 258, 337, 539
HgaI GACGC 3 cut(s) 8, 228, 524
HhaI GCGC 1 cut(s) 765
Hin1I GRCGYC 1 cut(s) 239
Hin1II CATG 3 cut(s) 88, 184, 975
Hin6I GCGC 1 cut(s) 763
HinP1I GCGC 1 cut(s) 763
HinfI GANTC 5 cut(s) 286, 332, 440, 578, 679
HpaII CCGG 4 cut(s) 221, 258, 337, 539
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 7 cut(s) 152, 172, 252, 361, 439, 577, 834
Hpy188III TCNNGA 4 cut(s) 96, 499, 539, 673
Hpy8I GTNNAC 1 cut(s) 196
HpyAV CCTTC 5 cut(s) 59, 202, 520, 829, 914
HpyCH4III ACNGT 2 cut(s) 365, 695
HpyCH4IV ACGT 1 cut(s) 522
HpyCH4V TGCA 6 cut(s) 196, 293, 355, 368, 462, 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 566, 613, 860, 892
HpyF3I CTNAG 2 cut(s) 56, 169
HpySE526I ACGT 1 cut(s) 522
Hsp92I GRCGYC 1 cut(s) 239
Hsp92II CATG 3 cut(s) 88, 184, 975
HspAI GCGC 1 cut(s) 763
Kpn2I TCCGGA 1 cut(s) 538
Kzo9I GATC 5 cut(s) 73, 152, 732, 799, 954
LmnI GCTCC 4 cut(s) 34, 532, 613, 706
Lsp1109I GCAGC 6 cut(s) 73, 94, 97, 107, 474, 879
MaeI CTAG 4 cut(s) 11, 243, 768, 864
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 2 cut(s) 13, 260
MalI GATC 5 cut(s) 75, 154, 734, 801, 956
MbiI CCGCTC 1 cut(s) 886
MboI GATC 5 cut(s) 73, 152, 732, 799, 954
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 198
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 4 cut(s) 38, 253, 372, 959
MluI ACGCGT 1 cut(s) 17
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 2 cut(s) 295, 341
MmeI TCCRAC 1 cut(s) 384
Mox20I TGGCCA 1 cut(s) 185
MroI TCCGGA 1 cut(s) 538
MscI TGGCCA 1 cut(s) 185
MseI TTAA 3 cut(s) 129, 926, 951
MslI CAYNNNNRTG 2 cut(s) 83, 313
Msp20I TGGCCA 1 cut(s) 185
MspI CCGG 4 cut(s) 221, 258, 337, 539
MspR9I CCNGG 3 cut(s) 337, 338, 737
Mva1269I GAATGC 1 cut(s) 78
MvaI CCWGG 1 cut(s) 737
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 4 cut(s) 566, 613, 860, 892
NciI CCSGG 2 cut(s) 337, 338
NdeII GATC 5 cut(s) 73, 152, 732, 799, 954
NlaIII CATG 3 cut(s) 88, 184, 975
NlaIV GGNNCC 2 cut(s) 154, 166
NmeAIII GCCGAG 1 cut(s) 407
NmuCI GTSAC 1 cut(s) 260
PctI GAATGC 1 cut(s) 78
PfeI GAWTC 3 cut(s) 440, 578, 679
PflFI GACNNNGTC 1 cut(s) 695
PkrI GCNGC 7 cut(s) 7, 63, 109, 112, 122, 464, 894
PleI GAGTC 2 cut(s) 294, 340
PmaCI CACGTG 1 cut(s) 523
PmlI CACGTG 1 cut(s) 523
PpsI GAGTC 2 cut(s) 294, 340
Ppu21I YACGTR 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 735
PspCI CACGTG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 735
PspN4I GGNNCC 2 cut(s) 154, 166
PspPI GGNCC 1 cut(s) 164
PstNI CAGNNNCTG 1 cut(s) 67
PsuI RGATCY 1 cut(s) 152
PsyI GACNNNGTC 1 cut(s) 695
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 2 cut(s) 83, 313
SaqAI TTAA 3 cut(s) 129, 926, 951
SatI GCNGC 7 cut(s) 6, 62, 108, 111, 121, 463, 893
Sau3AI GATC 5 cut(s) 73, 152, 732, 799, 954
Sau96I GGNCC 1 cut(s) 164
ScaI AGTACT 1 cut(s) 136
SchI GAGTC 2 cut(s) 295, 341
ScrFI CCNGG 3 cut(s) 337, 338, 737
SduI GDGCHC 1 cut(s) 198
SetI ASST 5 cut(s) 425, 525, 630, 687, 945
SinI GGWCC 1 cut(s) 164
SmaI CCCGGG 1 cut(s) 338
SmiMI CAYNNNNRTG 2 cut(s) 83, 313
SmlI CTYRAG 2 cut(s) 114, 298
SmoI CTYRAG 2 cut(s) 114, 298
Sse9I AATT 4 cut(s) 38, 253, 372, 959
SsiI CCGC 4 cut(s) 279, 757, 773, 886
SspMI CTAG 4 cut(s) 11, 243, 768, 864
StyD4I CCNGG 3 cut(s) 335, 336, 735
StyI CCWWGG 1 cut(s) 767
TaaI ACNGT 2 cut(s) 365, 695
TaiI ACGT 1 cut(s) 525
TaqI TCGA 4 cut(s) 42, 500, 674, 897
TaqII GACCGA 1 cut(s) 152
TasI AATT 4 cut(s) 38, 253, 372, 959
TatI WGTACW 1 cut(s) 134
TfiI GAWTC 3 cut(s) 440, 578, 679
Tru1I TTAA 3 cut(s) 129, 926, 951
Tru9I TTAA 3 cut(s) 129, 926, 951
TscAI CASTG 1 cut(s) 370
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 7 cut(s) 5, 61, 107, 110, 120, 462, 892
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 3 cut(s) 101, 221, 891
TspGWI ACGGA 3 cut(s) 47, 484, 658
TspMI CCCGGG 1 cut(s) 336
TspRI CASTG 1 cut(s) 370
Tth111I GACNNNGTC 1 cut(s) 695
VneI GTGCAC 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 2 cut(s) 253, 372
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XmaI CCCGGG 1 cut(s) 336
XmaJI CCTAGG 1 cut(s) 767
XspI CTAG 4 cut(s) 11, 243, 768, 864
ZrmI AGTACT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.