RLG00000025421

Protein SPT2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004232
Physical Location & Seq
Forward (+)
45682223 .. 45683185
963 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025421

Sequence Viewer

Length: 963 bp
ATGTCGGTGACGACGATGGGTGTTGCAGGTAACATGGGTATTCCTGAACTCCGGCAAATTCTGAAAGAGCGATACAGAATCGCTAATAACGTGAAAAGCTGCTCGGCTGAGATGAAAGAGACCAAGAATTCATCCTTGTTTGGGTCCTCGGAGTACTCTCTTTCACAAAGACTGATCGACGAGAGTAAAGCTCTTATGCCGGAGATTATCAATATTCTGGAGAGGACGAACAACAATGATCATCAAAAGCCCACAAAAGCAACAAAGCAGAATTCCGGTGTGGATTTGAAGCTTCAGCAAGCTAAGAAGGTGAAGGAATCGCGTGATTACTCTTTCTTGTTATCAGAGACTTCAGAAGTTCCGAAGATTTCGAAAACGTCACCACCAAAGTCTATTCCTAAAGCTGATGGGAACGGGATCCCAGAGAAGAGTCATGATAAGGTGTCTTCTCTGAAACCAAAGAAGAGTGGTGATGATAACAAGAAGTCCAAAGTCTCCTCGGCAAAGTCAGAATCCGTTTCGAAAGATTCAGTTCCGGTTTCTTGTTCAAAGAGTTTGAAGTCTCGGCCTAGTGAGAACATGGTGACAAAGTCACAAGCTTCGTCTACAAAGCAAAGCTTGAACCCGAAAAAGGAAATGATGCTGAAAAAAGAAGTTCCTGGCTTGAGGAAAAAACTGATCAAGAAACGCCGAGTGGAAGAGGATTTGGGCGATGATGGCAATGTAGACTTTTCTTCACTAATCCAAGATATTATGGGGAGAAGAGGAAAGAGGCGATACAGGGATTACGGTGACGACGGCGATGATCGTGCTATGGTGTCTGGCTTTAATGATATTCTGAAAGAAGAGAGGAAGAGTGCAATGATTGCGAGGAAGGAGGATGCAATAGAGGCTCTAAGGCTTGAACAAGAAGCGAAAGAAGAGAGGTTGAGCAAAGCAAAGAAGCAGAAGTTGAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPT2

Protein Analysis

321

Amino Acids

35.81

Weight (kDa)

9.72

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT2 PF08243 233 - 312 2.1e-12 SPT2 chromatin protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 17
AccI GTMKAC 2 cut(s) 605, 726
AccII CGCG 1 cut(s) 322
AclWI GGATC 2 cut(s) 412, 425
AcsI RAATTY 3 cut(s) 57, 127, 271
AcuI CTGAAG 2 cut(s) 278, 336
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 2 cut(s) 141, 631
AgsI TTSAA 6 cut(s) 289, 549, 559, 622, 905, 955
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 7 cut(s) 99, 191, 292, 302, 404, 599, 618
AluI AGCT 7 cut(s) 99, 191, 292, 302, 404, 599, 618
Alw26I GTCTC 4 cut(s) 113, 341, 499, 567
AlwI GGATC 2 cut(s) 412, 425
AoxI GGCC 1 cut(s) 566
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 3 cut(s) 57, 127, 271
AspS9I GGNCC 1 cut(s) 144
AsuHPI GGTGA 6 cut(s) 19, 322, 372, 482, 595, 803
AsuII TTCGAA 2 cut(s) 371, 521
AvaII GGWCC 1 cut(s) 144
BamHI GGATCC 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 1 cut(s) 86
BccI CCATC 3 cut(s) 10, 401, 710
BceAI ACGGC 1 cut(s) 814
BcgI CGANNNNNNTGC 2 cut(s) 791, 825
BciT130I CCWGG 1 cut(s) 660
BclI TGATCA 2 cut(s) 238, 678
BcoDI GTCTC 4 cut(s) 113, 341, 499, 567
BfaI CTAG 1 cut(s) 570
BfuAI ACCTGC 1 cut(s) 17
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 1 cut(s) 660
Bme18I GGWCC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 2 cut(s) 145, 419
BmrFI CCNGG 1 cut(s) 660
BmsI GCATC 2 cut(s) 630, 871
BpiI GAAGAC 1 cut(s) 438
BplI GAGNNNNNCTC 2 cut(s) 175, 207
BpmI CTGGAG 1 cut(s) 239
Bpu14I TTCGAA 2 cut(s) 371, 521
BpuEI CTTGAG 1 cut(s) 685
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 147, 498
BsaWI WCCGGW 2 cut(s) 275, 535
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 631
Bse3DI GCAATG 2 cut(s) 727, 867
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 2 cut(s) 147, 498
BseGI GGATG 2 cut(s) 131, 886
BseLI CCNNNNNNNGG 2 cut(s) 141, 631
BseMI GCAATG 2 cut(s) 727, 867
BseMII CTCAG 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 487
BseXI GCAGC 1 cut(s) 86
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 1 cut(s) 568
BsiSI CCGG 4 cut(s) 52, 200, 276, 536
BslI CCNNNNNNNGG 2 cut(s) 141, 631
BsmAI GTCTC 4 cut(s) 113, 341, 499, 567
BsnI GGCC 1 cut(s) 568
Bso31I GGTCTC 1 cut(s) 113
Bsp119I TTCGAA 2 cut(s) 371, 521
Bsp143I GATC 5 cut(s) 174, 238, 417, 678, 805
BspANI GGCC 1 cut(s) 568
BspCNI CTCAG 1 cut(s) 100
BspFNI CGCG 1 cut(s) 322
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 2 cut(s) 145, 419
BspMI ACCTGC 1 cut(s) 17
BspPI GGATC 2 cut(s) 412, 425
BspT104I TTCGAA 2 cut(s) 371, 521
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 2 cut(s) 727, 867
BssECI CCNNGG 2 cut(s) 147, 498
BssMI GATC 5 cut(s) 174, 238, 417, 678, 805
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 1 cut(s) 791
Bst6I CTCTTC 7 cut(s) 422, 458, 693, 757, 840, 848, 915
BstAPI GCANNNNNTGC 1 cut(s) 866
BstBI TTCGAA 2 cut(s) 371, 521
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 3 cut(s) 108, 303, 896
BstF5I GGATG 2 cut(s) 131, 886
BstFNI CGCG 1 cut(s) 322
BstKTI GATC 5 cut(s) 177, 241, 420, 681, 808
BstMAI GTCTC 4 cut(s) 113, 341, 499, 567
BstMBI GATC 5 cut(s) 174, 238, 417, 678, 805
BstMWI GCNNNNNNNGC 3 cut(s) 717, 866, 890
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 658
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 86
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
BsuRI GGCC 1 cut(s) 568
BtgZI GCGATG 2 cut(s) 726, 816
BtsCI GGATG 2 cut(s) 131, 886
BveI ACCTGC 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 300
CciI TCATGA 1 cut(s) 433
Cfr13I GGNCC 1 cut(s) 144
Csp6I GTAC 1 cut(s) 154
CviAII CATG 3 cut(s) 34, 434, 580
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 3 cut(s) 108, 303, 896
DpnI GATC 5 cut(s) 176, 240, 419, 680, 807
DpnII GATC 5 cut(s) 174, 238, 417, 678, 805
Eam1104I CTCTTC 7 cut(s) 422, 458, 693, 757, 840, 848, 915
EarI CTCTTC 7 cut(s) 422, 458, 693, 757, 840, 848, 915
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 1 cut(s) 144
Eco57I CTGAAG 2 cut(s) 278, 336
EcoO109I RGGNCCY 1 cut(s) 144
EcoRI GAATTC 2 cut(s) 127, 271
EcoRII CCWGG 1 cut(s) 658
FaeI CATG 3 cut(s) 37, 437, 583
FaiI YATR 6 cut(s) 35, 197, 435, 581, 755, 815
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FatI CATG 3 cut(s) 33, 433, 579
FbaI TGATCA 2 cut(s) 238, 678
FblI GTMKAC 2 cut(s) 605, 726
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 2 cut(s) 118, 893
Fsp4HI GCNGC 1 cut(s) 100
FspBI CTAG 1 cut(s) 570
GluI GCNGC 1 cut(s) 100
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 1 cut(s) 568
HapII CCGG 4 cut(s) 52, 200, 276, 536
Hin1II CATG 3 cut(s) 37, 437, 583
HindIII AAGCTT 3 cut(s) 290, 597, 616
HinfI GANTC 5 cut(s) 78, 317, 430, 512, 527
HpaII CCGG 4 cut(s) 52, 200, 276, 536
HphI GGTGA 6 cut(s) 19, 322, 372, 482, 595, 803
Hpy166II GTNNAC 2 cut(s) 606, 727
Hpy188I TCNGA 8 cut(s) 63, 151, 346, 355, 363, 453, 511, 840
Hpy188III TCNNGA 4 cut(s) 44, 218, 434, 682
Hpy8I GTNNAC 2 cut(s) 606, 727
Hpy99I CGWCG 3 cut(s) 16, 182, 800
HpyAV CCTTC 3 cut(s) 301, 307, 868
HpyCH4III ACNGT 1 cut(s) 791
HpyCH4IV ACGT 2 cut(s) 90, 377
HpyCH4V TGCA 3 cut(s) 26, 860, 884
HpyF10VI GCNNNNNNNGC 3 cut(s) 717, 866, 890
HpyF3I CTNAG 3 cut(s) 108, 303, 896
HpySE526I ACGT 2 cut(s) 90, 377
Hsp92II CATG 3 cut(s) 37, 437, 583
Ksp22I TGATCA 2 cut(s) 238, 678
Kzo9I GATC 5 cut(s) 174, 238, 417, 678, 805
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 2 cut(s) 630, 871
MaeI CTAG 1 cut(s) 570
MaeII ACGT 2 cut(s) 90, 377
MaeIII GTNAC 6 cut(s) 7, 29, 378, 583, 591, 791
MalI GATC 5 cut(s) 176, 240, 419, 680, 807
MboI GATC 5 cut(s) 174, 238, 417, 678, 805
MflI RGATCY 1 cut(s) 417
MluCI AATT 4 cut(s) 57, 127, 271, 958
MlyI GAGTC 1 cut(s) 439
MseI TTAA 1 cut(s) 828
MspI CCGG 4 cut(s) 52, 200, 276, 536
MspR9I CCNGG 1 cut(s) 660
MvaI CCWGG 1 cut(s) 660
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 3 cut(s) 717, 866, 890
NdeII GATC 5 cut(s) 174, 238, 417, 678, 805
NlaIII CATG 3 cut(s) 37, 437, 583
NlaIV GGNNCC 2 cut(s) 145, 419
NmeAIII GCCGAG 4 cut(s) 83, 479, 544, 716
NmuCI GTSAC 5 cut(s) 7, 378, 583, 591, 791
NspV TTCGAA 2 cut(s) 371, 521
PagI TCATGA 1 cut(s) 433
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 87
PfeI GAWTC 4 cut(s) 78, 317, 512, 527
PflFI GACNNNGTC 1 cut(s) 589
PkrI GCNGC 1 cut(s) 101
PleI GAGTC 1 cut(s) 438
PpsI GAGTC 1 cut(s) 438
PpuMI RGGWCCY 1 cut(s) 144
Psp5II RGGWCCY 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 2 cut(s) 145, 419
PspPI GGNCC 1 cut(s) 144
PspPPI RGGWCCY 1 cut(s) 144
PsuI RGATCY 1 cut(s) 417
PsyI GACNNNGTC 1 cut(s) 589
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 828
SatI GCNGC 1 cut(s) 100
Sau3AI GATC 5 cut(s) 174, 238, 417, 678, 805
Sau96I GGNCC 1 cut(s) 144
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 1 cut(s) 439
ScrFI CCNGG 1 cut(s) 660
SfaNI GCATC 2 cut(s) 630, 871
SfuI TTCGAA 2 cut(s) 371, 521
SinI GGWCC 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 664
SmoI CTYRAG 1 cut(s) 664
Sse9I AATT 4 cut(s) 57, 127, 271, 958
SspI AATATT 1 cut(s) 214
SspMI CTAG 1 cut(s) 570
StyD4I CCNGG 1 cut(s) 658
TaaI ACNGT 1 cut(s) 791
TaiI ACGT 2 cut(s) 93, 380
TaqI TCGA 3 cut(s) 177, 371, 521
TasI AATT 4 cut(s) 57, 127, 271, 958
TatI WGTACW 1 cut(s) 153
TfiI GAWTC 4 cut(s) 78, 317, 512, 527
Tru1I TTAA 1 cut(s) 828
Tru9I TTAA 1 cut(s) 828
TseFI GTSAC 5 cut(s) 7, 378, 583, 591, 791
TseI GCWGC 1 cut(s) 99
Tsp45I GTSAC 5 cut(s) 7, 378, 583, 591, 791
TspDTI ATGAA 2 cut(s) 120, 128
TspGWI ACGGA 1 cut(s) 505
Tth111I GACNNNGTC 1 cut(s) 589
VpaK11BI GGWCC 1 cut(s) 144
XapI RAATTY 3 cut(s) 57, 127, 271
XmiI GTMKAC 2 cut(s) 605, 726
XspI CTAG 1 cut(s) 570
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.