Rroxscaffold_6G00424820

Protein SPT2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004232
Physical Location & Seq
Forward (+)
45956604 .. 45957584
981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424820.1

Sequence Viewer

Length: 981 bp
ATGTCGATGACGACGATGGGTGTTGCAGGTAACATGGGTATTCCTGAACTCCGGCAAATTCTGAAAGAGCGATACAGAATTGCTAATAACATGAAAAGCTGCTCGGCTGAGATGAAAGAGACCAAGAACTCTTCCTTGTTTGGATCCTTGGAGTACTCTCTTTCACAAAGACTGATCGACGAGAGTAAAGCTCTTATGCCGGAGATTATCAATATTCTGGAGAAGACGAACAACAATGATCATCAAAAGCCCACAAAAGCAACAAAGCAGAATTCCGGCGTGGATTTGAAGCTTCAGCGAGCTAAGAAGGTGAAGGAATCGCGTGATTACTCTTTCTTGTTATCAGAGACTTCAGAAGTTCCGAAGATTTCAAAAGCGTCACCACCAATGTCTATTCCTAAAGCTGATGGTAATGGGATCCCAAAGAAGACTCATGATAAGGCGTCTTCTCTGAAACCAAAGAAGAGTGGTGATGATAACAACAAGTCCAAAGTCTCCTCGGCAAAGTCAGAGTCTGTTTCGAAAGATTCAGTTCTGGTTTCTGGTTCAGAGAGTTTGAAGTCTCGGTCTCGTGAGAACATGGTGACAAAGTCACAAGTTTCATCTACAAAGCGAAGCTTGAACCCGAAACAAGCAGAGCTCTGTAACACAAAAGTGATGCCGAAAAAGGAAGGTCCTTGCCTGAAGAAAAAAATGATGAAGAAACGCCGAGTCGAAGAGGACTTGGGGGATGATGGCAATGTAGACTATTCTTCACTAATCCAAGATATTATGGGGAGAAGAGGAAAGAGGCAATACAGGAATTATGGTGACGCCGGCGATGATCGTGCTATGGTGTCTGGCTTTAATGATATTCTGAAAGAAGAGAGGAGGAGTGCAGAGATTGCGAGGAAGGAGGATGCAATAGAGGCTCTAAGGCTTGAGCAAGAAGCCAAAGAAGAGAGGTTGAGCAAAGCAAAGAAGCAGAAGTTGAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPT2

Protein Analysis

326

Amino Acids

36.5

Weight (kDa)

9.72

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT2 PF08243 238 - 318 1.5e-12 SPT2 chromatin protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 744
AccII CGCG 1 cut(s) 322
AclWI GGATC 4 cut(s) 138, 151, 412, 425
AcsI RAATTY 2 cut(s) 57, 271
AcuI CTGAAG 3 cut(s) 278, 336, 704
AcyI GRCGYC 2 cut(s) 443, 813
AfaI GTAC 1 cut(s) 155
AgsI TTSAA 5 cut(s) 289, 372, 559, 622, 973
AleI CACNNNNGTG 1 cut(s) 653
AluBI AGCT 7 cut(s) 99, 191, 292, 302, 404, 618, 640
AluI AGCT 7 cut(s) 99, 191, 292, 302, 404, 618, 640
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 5 cut(s) 113, 341, 499, 567, 573
AlwI GGATC 4 cut(s) 138, 151, 412, 425
AlwNI CAGNNNCTG 1 cut(s) 515
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 2 cut(s) 57, 271
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
AspS9I GGNCC 1 cut(s) 674
AsuHPI GGTGA 5 cut(s) 322, 372, 482, 595, 821
AsuII TTCGAA 1 cut(s) 521
AvaII GGWCC 1 cut(s) 674
BamHI GGATCC 2 cut(s) 143, 417
BanII GRGCYC 1 cut(s) 642
BauI CACGAG 1 cut(s) 570
BbsI GAAGAC 3 cut(s) 230, 434, 438
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 1 cut(s) 86
BccI CCATC 3 cut(s) 10, 401, 728
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 5 cut(s) 113, 341, 499, 567, 573
BfuAI ACCTGC 1 cut(s) 17
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
BmcAI AGTACT 1 cut(s) 155
Bme18I GGWCC 1 cut(s) 674
BmgT120I GGNCC 1 cut(s) 674
BmiI GGNNCC 2 cut(s) 145, 419
BmsI GCATC 2 cut(s) 648, 889
BpiI GAAGAC 3 cut(s) 230, 434, 438
BplI GAGNNNNNCTC 2 cut(s) 175, 207
BpmI CTGGAG 1 cut(s) 239
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 1 cut(s) 941
BsaHI GRCGYC 2 cut(s) 443, 813
BsaI GGTCTC 2 cut(s) 113, 573
BsaJI CCNNGG 2 cut(s) 147, 498
Bse118I RCCGGY 1 cut(s) 815
Bse3DI GCAATG 1 cut(s) 745
BseDI CCNNGG 2 cut(s) 147, 498
BseGI GGATG 2 cut(s) 736, 904
BseMI GCAATG 1 cut(s) 745
BseMII CTCAG 1 cut(s) 99
BseRI GAGGAG 3 cut(s) 487, 883, 886
BseXI GCAGC 1 cut(s) 86
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 1 cut(s) 322
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 4 cut(s) 52, 200, 276, 816
BsmAI GTCTC 5 cut(s) 113, 341, 499, 567, 573
Bso31I GGTCTC 2 cut(s) 113, 573
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 5 cut(s) 143, 174, 238, 417, 823
BspCNI CTCAG 1 cut(s) 100
BspFNI CGCG 1 cut(s) 322
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 2 cut(s) 145, 419
BspMI ACCTGC 1 cut(s) 17
BspPI GGATC 4 cut(s) 138, 151, 412, 425
BspT104I TTCGAA 1 cut(s) 521
BspTNI GGTCTC 2 cut(s) 113, 573
BsrDI GCAATG 1 cut(s) 745
BsrFI RCCGGY 1 cut(s) 815
BssAI RCCGGY 1 cut(s) 815
BssECI CCNNGG 2 cut(s) 147, 498
BssMI GATC 5 cut(s) 143, 174, 238, 417, 823
BssNI GRCGYC 2 cut(s) 443, 813
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 147
Bst2BI CACGAG 1 cut(s) 570
Bst6I CTCTTC 6 cut(s) 136, 458, 711, 775, 858, 933
BstACI GRCGYC 2 cut(s) 443, 813
BstAPI GCANNNNNTGC 1 cut(s) 884
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 300, 817
BstDEI CTNAG 3 cut(s) 108, 303, 914
BstF5I GGATG 2 cut(s) 736, 904
BstFNI CGCG 1 cut(s) 322
BstKTI GATC 5 cut(s) 146, 177, 241, 420, 826
BstMAI GTCTC 5 cut(s) 113, 341, 499, 567, 573
BstMBI GATC 5 cut(s) 143, 174, 238, 417, 823
BstMWI GCNNNNNNNGC 2 cut(s) 884, 908
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 86
BstV2I GAAGAC 3 cut(s) 230, 434, 438
BstX2I RGATCY 2 cut(s) 143, 417
BstYI RGATCY 2 cut(s) 143, 417
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 2 cut(s) 736, 904
BveI ACCTGC 1 cut(s) 17
Cac8I GCNNGC 2 cut(s) 300, 817
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 433
Cfr10I RCCGGY 1 cut(s) 815
Cfr13I GGNCC 1 cut(s) 674
CseI GACGC 3 cut(s) 366, 432, 821
Csp6I GTAC 1 cut(s) 154
CviAII CATG 4 cut(s) 34, 91, 434, 580
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 3 cut(s) 108, 303, 914
DpnI GATC 5 cut(s) 145, 176, 240, 419, 825
DpnII GATC 5 cut(s) 143, 174, 238, 417, 823
Eam1104I CTCTTC 6 cut(s) 136, 458, 711, 775, 858, 933
EarI CTCTTC 6 cut(s) 136, 458, 711, 775, 858, 933
Ecl136II GAGCTC 1 cut(s) 640
Eco130I CCWWGG 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 2 cut(s) 113, 573
Eco47I GGWCC 1 cut(s) 674
Eco53kI GAGCTC 1 cut(s) 640
Eco57I CTGAAG 3 cut(s) 278, 336, 704
EcoICRI GAGCTC 1 cut(s) 640
EcoO109I RGGNCCY 1 cut(s) 674
EcoRI GAATTC 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 147
EcoT38I GRGCYC 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 4 cut(s) 37, 94, 437, 583
FaiI YATR 8 cut(s) 35, 92, 197, 435, 581, 773, 807, 833
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FatI CATG 4 cut(s) 33, 90, 433, 579
FbaI TGATCA 1 cut(s) 238
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 2 cut(s) 743, 911
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 1 cut(s) 100
GluI GCNGC 1 cut(s) 100
GsuI CTGGAG 1 cut(s) 239
HapII CCGG 4 cut(s) 52, 200, 276, 816
HgaI GACGC 3 cut(s) 366, 432, 821
Hin1I GRCGYC 2 cut(s) 443, 813
Hin1II CATG 4 cut(s) 37, 94, 437, 583
HindIII AAGCTT 2 cut(s) 290, 616
HinfI GANTC 5 cut(s) 317, 430, 512, 527, 711
HpaII CCGG 4 cut(s) 52, 200, 276, 816
HphI GGTGA 5 cut(s) 322, 372, 482, 595, 821
Hpy166II GTNNAC 1 cut(s) 745
Hpy188I TCNGA 8 cut(s) 63, 346, 355, 363, 453, 511, 550, 858
Hpy188III TCNNGA 4 cut(s) 44, 218, 434, 572
Hpy8I GTNNAC 1 cut(s) 745
Hpy99I CGWCG 2 cut(s) 16, 182
HpyAV CCTTC 4 cut(s) 301, 307, 665, 886
HpyCH4V TGCA 3 cut(s) 26, 878, 902
HpyF10VI GCNNNNNNNGC 2 cut(s) 884, 908
HpyF3I CTNAG 3 cut(s) 108, 303, 914
Hsp92I GRCGYC 2 cut(s) 443, 813
Hsp92II CATG 4 cut(s) 37, 94, 437, 583
KroI GCCGGC 1 cut(s) 815
KroNI GCCGGC 1 cut(s) 817
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 5 cut(s) 143, 174, 238, 417, 823
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 2 cut(s) 648, 889
MaeIII GTNAC 6 cut(s) 29, 378, 583, 591, 644, 809
MalI GATC 5 cut(s) 145, 176, 240, 419, 825
MboI GATC 5 cut(s) 143, 174, 238, 417, 823
MflI RGATCY 2 cut(s) 143, 417
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 5 cut(s) 57, 78, 271, 802, 976
MlyI GAGTC 3 cut(s) 424, 521, 720
MreI CGCCGGCG 1 cut(s) 815
MroNI GCCGGC 1 cut(s) 815
MseI TTAA 1 cut(s) 846
MslI CAYNNNNRTG 1 cut(s) 653
MspI CCGG 4 cut(s) 52, 200, 276, 816
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 884, 908
NaeI GCCGGC 1 cut(s) 817
NdeII GATC 5 cut(s) 143, 174, 238, 417, 823
NgoMIV GCCGGC 1 cut(s) 815
NlaIII CATG 4 cut(s) 37, 94, 437, 583
NlaIV GGNNCC 2 cut(s) 145, 419
NmeAIII GCCGAG 3 cut(s) 83, 479, 734
NmuCI GTSAC 4 cut(s) 378, 583, 591, 809
NspV TTCGAA 1 cut(s) 521
OliI CACNNNNGTG 1 cut(s) 653
PagI TCATGA 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdiI GCCGGC 1 cut(s) 817
PfeI GAWTC 2 cut(s) 317, 527
PflFI GACNNNGTC 1 cut(s) 589
PkrI GCNGC 1 cut(s) 101
PleI GAGTC 3 cut(s) 424, 520, 719
PpsI GAGTC 3 cut(s) 424, 520, 719
PpuMI RGGWCCY 1 cut(s) 674
Psp124BI GAGCTC 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 674
PspN4I GGNNCC 2 cut(s) 145, 419
PspPI GGNCC 1 cut(s) 674
PspPPI RGGWCCY 1 cut(s) 674
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 2 cut(s) 143, 417
PsyI GACNNNGTC 1 cut(s) 589
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 653
SacI GAGCTC 1 cut(s) 642
SaqAI TTAA 1 cut(s) 846
SatI GCNGC 1 cut(s) 100
Sau3AI GATC 5 cut(s) 143, 174, 238, 417, 823
Sau96I GGNCC 1 cut(s) 674
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 3 cut(s) 424, 521, 720
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 2 cut(s) 648, 889
SfuI TTCGAA 1 cut(s) 521
SgrAI CRCCGGYG 1 cut(s) 815
SinI GGWCC 1 cut(s) 674
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
Sse9I AATT 5 cut(s) 57, 78, 271, 802, 976
SspI AATATT 1 cut(s) 214
SstI GAGCTC 1 cut(s) 642
StyI CCWWGG 1 cut(s) 147
TaqI TCGA 4 cut(s) 5, 177, 521, 714
TaqII GACCGA 1 cut(s) 555
TasI AATT 5 cut(s) 57, 78, 271, 802, 976
TatI WGTACW 1 cut(s) 153
TfiI GAWTC 2 cut(s) 317, 527
Tru1I TTAA 1 cut(s) 846
Tru9I TTAA 1 cut(s) 846
TseFI GTSAC 4 cut(s) 378, 583, 591, 809
TseI GCWGC 1 cut(s) 99
Tsp45I GTSAC 4 cut(s) 378, 583, 591, 809
TspDTI ATGAA 4 cut(s) 107, 128, 591, 713
Tth111I GACNNNGTC 1 cut(s) 589
VpaK11BI GGWCC 1 cut(s) 674
XapI RAATTY 2 cut(s) 57, 271
XmiI GTMKAC 1 cut(s) 744
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.