pycom03g11960

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003273
Physical Location & Seq
Reverse (-)
12187602 .. 12189979
2378 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGGTTTTTGAAGTCAACGTTATAATCTATGTGTTGCAATGCCAACAGCTGCTAATAATCTCCAATCTGCTTGCGATAAACATGGAGCCCCATCGAAGCAAACCCATCTCAATTTCTTGTTTTCTTTTCTTTTTAGTTCTACTCGCGTATTCCGGATCACTCACAGCTCAAGAAGTCGAGGATGAAAGAGAGTTTGATTATCTTCAAGAGGGTGGAAAGGGGCCAAAGCATTGGGGGGAGATTAAGGAAGAATGGGCGGCGTGTAACAGCGGGAGCATGCAATCTCCAATAGATTTATCAAATCACAGGGTTCAATGGGTGGACGACGCTGGAACAATTAAGATAAATGGTACTGAGTATCCGCTAAAACAAGGTCACTGGCATGCCCCAGCGGAGCATTCCATCAACGGCAGAGTATATGACATGGAGCTCCACTTGGTTCATCTAAGCTCCGATCCCAACGTAGCAAACAAGATCGCCGTCACTGCAGTTCTCTACAAGTTCGGTCGTGCAGATTCTTTCCTTTCTACGGTACGTCCTCAACTAAACTCAAGTGTCCTAATTTGCTCACCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

193

Amino Acids

21.53

Weight (kDa)

5.55

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 117 - 171 5.5e-06 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 23
AccII CGCG 1 cut(s) 146
AccIII TCCGGA 1 cut(s) 152
AciI CCGC 4 cut(s) 257, 270, 362, 392
AclI AACGTT 1 cut(s) 18
AclWI GGATC 2 cut(s) 163, 449
AfaI GTAC 2 cut(s) 352, 534
AfiI CCNNNNNNNGG 1 cut(s) 529
AgsI TTSAA 3 cut(s) 11, 206, 314
AluBI AGCT 4 cut(s) 49, 167, 430, 450
AluI AGCT 4 cut(s) 49, 167, 430, 450
Alw21I GWGCWC 1 cut(s) 432
AlwI GGATC 2 cut(s) 163, 449
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 1 cut(s) 221
ApeKI GCWGC 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 561
BanII GRGCYC 2 cut(s) 90, 432
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 1 cut(s) 36
BccI CCATC 3 cut(s) 99, 113, 410
BceAI ACGGC 2 cut(s) 424, 464
BciVI GTATCC 1 cut(s) 369
BfmI CTRYAG 1 cut(s) 486
BfuI GTATCC 1 cut(s) 369
BisI GCNGC 2 cut(s) 50, 258
BlsI GCNGC 2 cut(s) 51, 259
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 2 cut(s) 87, 222
BpuEI CTTGAG 2 cut(s) 153, 535
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse1I ACTGG 1 cut(s) 383
Bse3DI GCAATG 1 cut(s) 44
BseAI TCCGGA 1 cut(s) 152
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMI GCAATG 1 cut(s) 44
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 1 cut(s) 383
BseXI GCAGC 1 cut(s) 36
BseYI CCCAGC 1 cut(s) 388
BsgI GTGCAG 1 cut(s) 531
Bsh1236I CGCG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 508
BshFI GGCC 1 cut(s) 223
BsiEI CGRYCG 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 529
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 1 cut(s) 223
Bsp1286I GDGCHC 2 cut(s) 90, 432
Bsp13I TCCGGA 1 cut(s) 152
Bsp143I GATC 3 cut(s) 155, 454, 474
BspACI CCGC 4 cut(s) 257, 270, 362, 392
BspANI GGCC 1 cut(s) 223
BspCNI CTCAG 1 cut(s) 346
BspEI TCCGGA 1 cut(s) 152
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 87, 222
BspMAI CTGCAG 1 cut(s) 490
BspPI GGATC 2 cut(s) 163, 449
BsrDI GCAATG 1 cut(s) 44
BsrI ACTGG 1 cut(s) 383
BssMI GATC 3 cut(s) 155, 454, 474
Bst4CI ACNGT 1 cut(s) 532
BstC8I GCNNGC 3 cut(s) 72, 278, 384
BstDEI CTNAG 2 cut(s) 354, 446
BstF5I GGATG 1 cut(s) 187
BstFNI CGCG 1 cut(s) 146
BstKTI GATC 3 cut(s) 158, 457, 477
BstMBI GATC 3 cut(s) 155, 454, 474
BstMCI CGRYCG 1 cut(s) 508
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNSI RCATGY 2 cut(s) 280, 386
BstSFI CTRYAG 1 cut(s) 486
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 36
BstXI CCANNNNNNTGG 1 cut(s) 231
BsuI GTATCC 1 cut(s) 369
BsuRI GGCC 1 cut(s) 223
BtsCI GGATG 1 cut(s) 187
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 376, 483
Cac8I GCNNGC 3 cut(s) 72, 278, 384
Cfr13I GGNCC 1 cut(s) 221
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 351, 533
CviAII CATG 5 cut(s) 82, 277, 383, 424, 573
CviJI RGCY 6 cut(s) 49, 88, 167, 223, 430, 450
CviKI_1 RGCY 6 cut(s) 49, 88, 167, 223, 430, 450
CviQI GTAC 2 cut(s) 351, 533
DdeI CTNAG 2 cut(s) 354, 446
DpnI GATC 3 cut(s) 157, 456, 476
DpnII GATC 3 cut(s) 155, 454, 474
Ecl136II GAGCTC 1 cut(s) 430
Eco24I GRGCYC 2 cut(s) 90, 432
Eco53kI GAGCTC 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 430
EcoT38I GRGCYC 2 cut(s) 90, 432
FaeI CATG 5 cut(s) 85, 280, 386, 427, 576
FaiI YATR 9 cut(s) 23, 30, 83, 278, 384, 418, 420, 425, 574
FatI CATG 5 cut(s) 81, 276, 382, 423, 572
FauI CCCGC 1 cut(s) 263
Fnu4HI GCNGC 2 cut(s) 50, 258
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 90, 432
Fsp4HI GCNGC 2 cut(s) 50, 258
GluI GCNGC 2 cut(s) 50, 258
GsaI CCCAGC 1 cut(s) 392
HaeIII GGCC 1 cut(s) 223
HapII CCGG 1 cut(s) 153
HgaI GACGC 1 cut(s) 335
Hin1II CATG 5 cut(s) 85, 280, 386, 427, 576
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 1 cut(s) 515
HpaII CCGG 1 cut(s) 153
HphI GGTGA 1 cut(s) 561
Hpy166II GTNNAC 2 cut(s) 16, 322
Hpy188I TCNGA 1 cut(s) 454
Hpy188III TCNNGA 3 cut(s) 153, 170, 206
Hpy8I GTNNAC 2 cut(s) 16, 322
Hpy99I CGWCG 1 cut(s) 329
HpyCH4III ACNGT 1 cut(s) 532
HpyCH4IV ACGT 3 cut(s) 18, 462, 535
HpyCH4V TGCA 4 cut(s) 37, 280, 488, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpyF3I CTNAG 2 cut(s) 354, 446
HpySE526I ACGT 3 cut(s) 18, 462, 535
Hsp92II CATG 5 cut(s) 85, 280, 386, 427, 576
Kpn2I TCCGGA 1 cut(s) 152
Kzo9I GATC 3 cut(s) 155, 454, 474
LmnI GCTCC 6 cut(s) 85, 273, 394, 427, 435, 455
LpnPI CCDG 5 cut(s) 166, 292, 315, 364, 402
Lsp1109I GCAGC 1 cut(s) 36
MaeII ACGT 3 cut(s) 18, 462, 535
MaeIII GTNAC 3 cut(s) 263, 374, 481
MalI GATC 3 cut(s) 157, 456, 476
MboI GATC 3 cut(s) 155, 454, 474
MboII GAAGA 2 cut(s) 194, 260
MhlI GDGCHC 2 cut(s) 90, 432
MluCI AATT 3 cut(s) 111, 336, 561
MnlI CCTC 3 cut(s) 172, 202, 549
MroI TCCGGA 1 cut(s) 152
MseI TTAA 3 cut(s) 243, 339, 577
MslI CAYNNNNRTG 1 cut(s) 381
MspA1I CMGCKG 3 cut(s) 49, 270, 392
MspI CCGG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 397
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 485
NdeII GATC 3 cut(s) 155, 454, 474
NlaIII CATG 5 cut(s) 85, 280, 386, 427, 576
NlaIV GGNNCC 2 cut(s) 87, 222
NmuCI GTSAC 2 cut(s) 374, 481
NspI RCATGY 2 cut(s) 280, 386
PaeI GCATGC 2 cut(s) 280, 386
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 1 cut(s) 515
PkrI GCNGC 2 cut(s) 51, 259
PsiI TTATAA 1 cut(s) 23
Psp124BI GAGCTC 1 cut(s) 432
Psp1406I AACGTT 1 cut(s) 18
PspFI CCCAGC 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 87, 222
PspPI GGNCC 1 cut(s) 221
PstI CTGCAG 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 2 cut(s) 352, 534
RsaNI GTAC 2 cut(s) 351, 533
RseI CAYNNNNRTG 1 cut(s) 381
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 3 cut(s) 243, 339, 577
SatI GCNGC 2 cut(s) 50, 258
Sau3AI GATC 3 cut(s) 155, 454, 474
Sau96I GGNCC 1 cut(s) 221
SduI GDGCHC 2 cut(s) 90, 432
SetI ASST 8 cut(s) 21, 51, 169, 376, 432, 452, 465, 538
SfcI CTRYAG 1 cut(s) 486
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 2 cut(s) 168, 550
SmoI CTYRAG 2 cut(s) 168, 550
SphI GCATGC 2 cut(s) 280, 386
Sse9I AATT 3 cut(s) 111, 336, 561
SsiI CCGC 4 cut(s) 257, 270, 362, 392
SstI GAGCTC 1 cut(s) 432
TaaI ACNGT 1 cut(s) 532
TaiI ACGT 3 cut(s) 21, 465, 538
TaqI TCGA 2 cut(s) 94, 177
TaqII GACCGA 1 cut(s) 494
TasI AATT 3 cut(s) 111, 336, 561
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 1 cut(s) 515
Tru1I TTAA 3 cut(s) 243, 339, 577
Tru9I TTAA 3 cut(s) 243, 339, 577
TscAI CASTG 2 cut(s) 383, 490
TseFI GTSAC 2 cut(s) 374, 481
TseI GCWGC 1 cut(s) 49
Tsp45I GTSAC 2 cut(s) 374, 481
TspDTI ATGAA 2 cut(s) 198, 431
TspRI CASTG 2 cut(s) 383, 490
XceI RCATGY 2 cut(s) 280, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.