Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0003132

Physical Location & Seq

Genomic Coordinates Reverse (-)

17261741 .. 17264601

Gene Structure 2861 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGTCATGCGCTCCTCCTCCGCTCGCCCCTGGGTTCGAGTTTGCCAGGTATATCTATACTATAAAAAATGGTAAAGCCGGGACGAAAGCGCAGTGTATTGAGAGTGCAATGAATGCATTGAAGGATGGGAAGAGTGTTTTTATAGATAGGTGTAATATGGAGAAAGAACAAAGGGATGAGTTTGTGAAGCTTGGTGGTAGTGCCCAGGTTGATGTTCATGCTGTGGTGCTTGATCTTCCTGCCAAGCTTTGTATATCGCGGTCTGTGAAGAGAACGGGCCACGAGGGAAACTTGCAAGGTGGGAGAGCTGCTGTGGTTGTGAATAGAATGCTGCAGAAGAAAGAACTGCCCAAACTGAGTGAAGGGTTTGCTAGAATCACTTGTTGCCAGAGTGAGAGTGATGTTCAATCTGCTGTTGATGCATATAGTGGACTTGGTCCGTTGGATACACTTCCCAGTGGTTATTTTGGCCAGAAGAACGCCGGTGCGAAAGTACAACTAGGTATAATGAGGTTCTTAAAGAAAACAGATGGTCCTGCTAATAGTGAATCCACTTCGAAGAGTGTACCAGATTCTAATGCTTCTCGAATTATTGAAGAAAAGGAAACCTCTTCGAAGGGAACAGGTTCATTGTCTGAGAATTCTCGTAAGGAGTCAAAGGAAGGTGGAGAGCTATTTGTAGGCTCTGTTGGCGGTGATGTTTCTCTAAACGATTCCCCCACTCTGGCATTTCCCTCCATATCGACAGCAGATTTTCAATTTGACATTGAGAAGGCGTCTGATATTATTGTCGAGAAAGTTATAGAATTTTTTAATAAGCTTGGAAATGCTAGGCTTGTTTTAGTGGACTTGAGTCATAAATCAAAGATTTTGTCTTTGGTAAGGGCTAAAGCTTCAGAGAAAAACATTAACTCCAACAAGTTCTTCACATTTGTGGGAGATATCACTCGGCTTCATTCAGGAGGAGGTTTACACTGCAATGTAATAGCTAATGCTGCTAATTGGCGTCTTAAACCTGGAGGTGGAGGTGTCAATGCTGCAATCTTCAGTGCTGGAGGTCCATCTTTGGAGGTTGCAACCAAAGAACAAGCCCAATCTCTGTATCCTGGGAGTGCTGTGGTTGTGCCTCTCCCTTCAATTTCACCCTTGTTTAGTAGGGAGGGGGTAACCCATGTCATACATGTTGTTGGACCCAACATGAACCCACACAGACCAAATTGTCTGAACAATGACTATAGTAAAGGCTGCAAAGTTCTCCGGGAGGCTTACACTTCACTTTTCGAAGGTTTCGCAACAATTGTGAGGACCCAAGCAAAGTCACCCAAGGGAAGCATTGAGAACCTGCAGGCAAAGCTGCCAAAGTCACAAGAACATTCTGATGCCTCTAGAAATCATTTTACAAACAGCGATCAAAAGACTAAGAGAGAGGATCCTCACAAATATGAGAGGAGCAAAAGAAGTAAAGCATATCAGGATGATACTGAAGATTCTAACACTGGTAAACCAAACCTGAGCAACAAGTCTGGTGGAAGCAGGACTAAATCCTGGGGGTCATGGGCTCAGGCTCTCTACAACACTGCTATGCACCCAGAGAAGCACAAGGATGCAGTGCTAGAGATATCAGATGATGTCATTGTGCTGAATGATCTTTATCCAAAGGCAAAAAGGCATGTTTTGGTGGTGGCACGATGTGCAGGTCTTGATCGACTGTCAGATGTTCATAAAGAACACCTTCCATTGTTAAGGACAATGCATGAAGTTGGTCTGAAGTGGGTTGAGAAATTCTTGCATGATGATTCATCTTTGGTCTTCCGTCTTGGATATCACTTGGATCCCTCAATGCGACAGCTGCATCTGCATGTTATCAGCCAGGATTTTGATTCAACATACTTGAAGAATAAGAAACATTGGAACTCCTTCAATACTGCTTTCTTTCGAGACTCAGTGGATGTGGTGGAGGAAGTCAGTAGTGATGGAAAAGCAAAGCTAAAAGAAGACGATAGCATGTTGTCTATGGAGTTGCGATGCCACCGATGTAGAAGCGCTCACCCCAACATACCCCGATTAAAATCTCACATAACTAACTGTCGAGCCACCTTTCCAAGCACCTTGCTCCAAAATGGTCGTCTTGTTCACGCACCAAGCAATGTCAGCATTGGTCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006139 GO:0006163 GO:0006725 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009117 GO:0009150 GO:0009259 GO:0009987 GO:0016787 GO:0016817 GO:0016819 GO:0019637 GO:0019693 GO:0034641 GO:0044237 GO:0044238 GO:0044281 GO:0046483 GO:0047627 GO:0055086 GO:0071704 GO:0072521 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_33 DcpS_C HLH Macro zf-C2HE

Protein Analysis

722

Amino Acids

79.06

Weight (kDa)

9.09

Isoelectric Point (pI)

42.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_33	PF13671	28 - 98	2.6e-06	AAA domain
Macro	PF01661	331 - 407	3.6e-07	Macro domain
DcpS_C	PF11969	524 - 636	2.4e-24	Scavenger mRNA decapping enzyme C-term binding
HIT	PF01230	535 - 625	1.3e-08	HIT domain
zf-C2HE	PF16278	641 - 695	1.1e-10	C2HE / C2H2 / C2HC zinc-binding finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01310
fragaria_vesca	FvH4_6g11090 FvH4_6g11090 FvH4_6g11090 FvH4_6g11090 FvH4_6g11090
malus_domestica	MD04G1160300.v1.1 MD05G1095500.v1.1 MD10G1110900.v1.1 MD12G1172800.v1.1
prunus_persica	Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1
pyrus_communis	pycom04g14360 pycom12g16400 pycom12g16440
rosa_chinensis	RchiOBHm_Chr3g0462501 RchiOBHm_Chr3g0462521
rosa_laevigata	RLG00000024853 RLG00000024857
rosa_multiflora	Rmu_sc0003624.1_g000009 Rmu_sc0003624.1_g000012 Rmu_sc0003624.1_g000015 Rmu_sc0011346.1_g000002 Rmu_sc0011346.1_g000005
rosa_roxburghii	Rroxscaffold_6G00417460
rosa_rugosa	Rorug03G0057000
rosa_samantha	Rh3AG115200 Rh3BG118500 Rh3BG118600 Rh3CG121400 Rh3CG122000 Rh3DG120700
rosa_wichuraiana	Rw0G003810 Rw3G009750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1218
Acc36I	ACCTGC	2 cut(s)	1350, 1685
AccBSI	CCGCTC	1 cut(s)	22
AccII	CGCG	1 cut(s)	259
AciI	CCGC	3 cut(s)	20, 259, 693
AclWI	GGATC	4 cut(s)	1424, 1437, 1826, 1839
AcoI	YGGCCR	1 cut(s)	469
AcsI	RAATTY	3 cut(s)	640, 806, 1781
AcuI	CTGAAG	4 cut(s)	879, 1030, 1503, 1787
AcyI	GRCGYC	2 cut(s)	776, 1006
AdeI	CACNNNGTG	1 cut(s)	1691
AfaI	GTAC	2 cut(s)	495, 567
AfeI	AGCGCT	1 cut(s)	2044
AfiI	CCNNNNNNNGG	2 cut(s)	724, 1022
AflIII	ACRYGT	1 cut(s)	1180
AgsI	TTSAA	8 cut(s)	121, 407, 596, 758, 1137, 1884, 1894, 1921
AjnI	CCWGG	7 cut(s)	28, 44, 204, 1015, 1105, 1544, 1869
AjuI	GAANNNNNNNTTGG	2 cut(s)	908, 940
AleI	CACNNNNGTG	1 cut(s)	932
Alw26I	GTCTC	1 cut(s)	1932
AlwI	GGATC	4 cut(s)	1424, 1437, 1826, 1839
Aor51HI	AGCGCT	1 cut(s)	2044
AoxI	GGCC	2 cut(s)	277, 469
ApeKI	GCWGC	7 cut(s)	308, 331, 995, 1037, 1245, 1354, 1849
ApoI	RAATTY	3 cut(s)	640, 806, 1781
ArsI	GACNNNNNNTTYG	4 cut(s)	857, 889, 2110, 2142
Asp700I	GAANNNNTTC	3 cut(s)	625, 1731, 1916
AspLEI	GCGC	3 cut(s)	11, 91, 2045
AspS9I	GGNCC	7 cut(s)	277, 437, 533, 1058, 1190, 1305, 2159
AsuC2I	CCSGG	2 cut(s)	79, 1259
AsuHPI	GGTGA	4 cut(s)	707, 1134, 1311, 2039
AsuII	TTCGAA	3 cut(s)	557, 614, 1281
AvaII	GGWCC	6 cut(s)	437, 533, 1058, 1190, 1305, 2159
BaeGI	GKGCMC	1 cut(s)	205
BalI	TGGCCA	1 cut(s)	471
BamHI	GGATCC	2 cut(s)	1429, 1831
BanII	GRGCYC	1 cut(s)	1561
BauI	CACGAG	1 cut(s)	281
BbsI	GAAGAC	2 cut(s)	1801, 2001
BbvI	GCAGC	1 cut(s)	727
BccI	CCATC	4 cut(s)	119, 524, 1069, 1967
BciT130I	CCWGG	7 cut(s)	30, 46, 206, 1017, 1107, 1546, 1871
BciVI	GTATCC	2 cut(s)	439, 1113
BcnI	CCSGG	2 cut(s)	79, 1259
BcoDI	GTCTC	1 cut(s)	1932
BfaI	CTAG	5 cut(s)	372, 500, 831, 1386, 1613
BfmI	CTRYAG	3 cut(s)	332, 1234, 1343
BfoI	RGCGCY	1 cut(s)	2046
BfuAI	ACCTGC	2 cut(s)	1350, 1685
BfuI	GTATCC	2 cut(s)	439, 1113
BisI	GCNGC	7 cut(s)	309, 332, 996, 1038, 1246, 1355, 1850
BlsI	GCNGC	7 cut(s)	310, 333, 997, 1039, 1247, 1356, 1851
Bme1390I	CCNGG	9 cut(s)	30, 46, 79, 206, 1017, 1107, 1259, 1546, 1871
Bme18I	GGWCC	6 cut(s)	437, 533, 1058, 1190, 1305, 2159
BmgT120I	GGNCC	7 cut(s)	277, 437, 533, 1058, 1190, 1305, 2159
BmiI	GGNNCC	4 cut(s)	1192, 1307, 1431, 1833
BmrFI	CCNGG	9 cut(s)	30, 46, 79, 206, 1017, 1107, 1259, 1546, 1871
BmrI	ACTGGG	1 cut(s)	450
BmsI	GCATC	5 cut(s)	409, 1369, 1594, 1861, 2015
BmuI	ACTGGG	1 cut(s)	450
BoxI	GACNNNNGTC	1 cut(s)	852
BpiI	GAAGAC	2 cut(s)	1801, 2001
BpmI	CTGGAG	2 cut(s)	1038, 1074
Bpu10I	CCTNAGC	2 cut(s)	1511, 1560
Bpu14I	TTCGAA	3 cut(s)	557, 614, 1281
BpuEI	CTTGAG	1 cut(s)	871
BpuMI	CCSGG	2 cut(s)	79, 1259
BsaBI	GATNNNNATC	2 cut(s)	1650, 2068
BsaHI	GRCGYC	2 cut(s)	776, 1006
BsaJI	CCNNGG	6 cut(s)	28, 29, 204, 1106, 1323, 1545
BsaXI	ACNNNNNCTCC	2 cut(s)	896, 926
Bsc4I	CCNNNNNNNGG	2 cut(s)	724, 1022
Bse118I	RCCGGY	1 cut(s)	482
Bse1I	ACTGG	2 cut(s)	456, 1501
Bse3DI	GCAATG	3 cut(s)	114, 985, 2152
Bse8I	GATNNNNATC	2 cut(s)	1650, 2068
BseBI	CCWGG	7 cut(s)	30, 46, 206, 1017, 1107, 1546, 1871
BseDI	CCNNGG	6 cut(s)	28, 29, 204, 1106, 1323, 1545
BseGI	GGATG	5 cut(s)	130, 181, 1480, 1609, 1954
BseJI	GATNNNNATC	2 cut(s)	1650, 2068
BseLI	CCNNNNNNNGG	2 cut(s)	724, 1022
BseMI	GCAATG	3 cut(s)	114, 985, 2152
BseMII	CTCAG	5 cut(s)	347, 627, 1502, 1574, 1956
BseNI	ACTGG	2 cut(s)	456, 1501
BseRI	GAGGAG	3 cut(s)	6, 978, 1462
BseSI	GKGCMC	1 cut(s)	205
BseXI	GCAGC	7 cut(s)	295, 318, 982, 1024, 1232, 1341, 1836
BsgI	GTGCAG	1 cut(s)	1713
Bsh1236I	CGCG	1 cut(s)	259
BshFI	GGCC	2 cut(s)	279, 471
BsiSI	CCGG	3 cut(s)	78, 483, 1258
BslFI	GGGAC	1 cut(s)	94
BslI	CCNNNNNNNGG	2 cut(s)	724, 1022
BsmAI	GTCTC	1 cut(s)	1932
BsmFI	GGGAC	1 cut(s)	94
BsmI	GAATGC	2 cut(s)	118, 333
BsnI	GGCC	2 cut(s)	279, 471
Bsp119I	TTCGAA	3 cut(s)	557, 614, 1281
Bsp1286I	GDGCHC	2 cut(s)	205, 1561
Bsp143I	GATC	6 cut(s)	232, 1408, 1429, 1645, 1702, 1831
BspACI	CCGC	3 cut(s)	20, 259, 693
BspANI	GGCC	2 cut(s)	279, 471
BspCNI	CTCAG	5 cut(s)	348, 628, 1503, 1573, 1955
BspFNI	CGCG	1 cut(s)	259
BspLI	GGNNCC	4 cut(s)	1192, 1307, 1431, 1833
BspMAI	CTGCAG	2 cut(s)	336, 1347
BspMI	ACCTGC	2 cut(s)	1350, 1685
BspPI	GGATC	4 cut(s)	1424, 1437, 1826, 1839
BspT104I	TTCGAA	3 cut(s)	557, 614, 1281
BsrBI	CCGCTC	1 cut(s)	22
BsrDI	GCAATG	3 cut(s)	114, 985, 2152
BsrFI	RCCGGY	1 cut(s)	482
BsrI	ACTGG	2 cut(s)	456, 1501
BssAI	RCCGGY	1 cut(s)	482
BssECI	CCNNGG	6 cut(s)	28, 29, 204, 1106, 1323, 1545
BssMI	GATC	6 cut(s)	232, 1408, 1429, 1645, 1702, 1831
BssNI	GRCGYC	2 cut(s)	776, 1006
BssSI	CACGAG	1 cut(s)	281
BssT1I	CCWWGG	1 cut(s)	1323
Bst2BI	CACGAG	1 cut(s)	281
Bst2UI	CCWGG	7 cut(s)	30, 46, 206, 1017, 1107, 1546, 1871
Bst4CI	ACNGT	2 cut(s)	1710, 2087
Bst6I	CTCTTC	4 cut(s)	125, 263, 554, 616
BstACI	GRCGYC	2 cut(s)	776, 1006
BstAPI	GCANNNNNTGC	2 cut(s)	113, 1691
BstBI	TTCGAA	3 cut(s)	557, 614, 1281
BstC8I	GCNNGC	2 cut(s)	24, 1347
BstDEI	CTNAG	6 cut(s)	356, 636, 1419, 1511, 1560, 1942
BstEII	GGTNACC	1 cut(s)	1165
BstF5I	GGATG	5 cut(s)	130, 181, 1480, 1609, 1954
BstFNI	CGCG	1 cut(s)	259
BstH2I	RGCGCY	1 cut(s)	2046
BstHHI	GCGC	3 cut(s)	11, 91, 2045
BstKTI	GATC	6 cut(s)	235, 1411, 1432, 1648, 1705, 1834
BstMAI	GTCTC	1 cut(s)	1932
BstMBI	GATC	6 cut(s)	232, 1408, 1429, 1645, 1702, 1831
BstMWI	GCNNNNNNNGC	9 cut(s)	113, 419, 690, 995, 1351, 1691, 1849, 1855, 2151
BstNI	CCWGG	7 cut(s)	30, 46, 206, 1017, 1107, 1546, 1871
BstNSI	RCATGY	4 cut(s)	1184, 1673, 1862, 2008
BstPAI	GACNNNNGTC	1 cut(s)	852
BstPI	GGTNACC	1 cut(s)	1165
BstSCI	CCNGG	9 cut(s)	28, 44, 77, 204, 1015, 1105, 1257, 1544, 1869
BstSFI	CTRYAG	3 cut(s)	332, 1234, 1343
BstSLI	GKGCMC	1 cut(s)	205
BstUI	CGCG	1 cut(s)	259
BstV1I	GCAGC	7 cut(s)	295, 318, 982, 1024, 1232, 1341, 1836
BstV2I	GAAGAC	2 cut(s)	1801, 2001
BstX2I	RGATCY	2 cut(s)	1429, 1831
BstYI	RGATCY	2 cut(s)	1429, 1831
BsuI	GTATCC	2 cut(s)	439, 1113
BsuRI	GGCC	2 cut(s)	279, 471
BtgZI	GCGATG	1 cut(s)	2038
BtsCI	GGATG	5 cut(s)	130, 181, 1480, 1609, 1954
BtsI	GCAGTG	4 cut(s)	98, 973, 1575, 1614
BtsIMutI	CAGTG	8 cut(s)	98, 463, 973, 1054, 1494, 1575, 1614, 1950
BveI	ACCTGC	2 cut(s)	1350, 1685
Cac8I	GCNNGC	2 cut(s)	24, 1347
CfoI	GCGC	3 cut(s)	11, 91, 2045
Cfr10I	RCCGGY	1 cut(s)	482
Cfr13I	GGNCC	7 cut(s)	277, 437, 533, 1058, 1190, 1305, 2159
CseI	GACGC	2 cut(s)	765, 995
Csp6I	GTAC	2 cut(s)	494, 566
CspCI	CAANNNNNGTGG	2 cut(s)	1507, 1542
CviQI	GTAC	2 cut(s)	494, 566
DdeI	CTNAG	6 cut(s)	356, 636, 1419, 1511, 1560, 1942
DpnI	GATC	6 cut(s)	234, 1410, 1431, 1647, 1704, 1833
DpnII	GATC	6 cut(s)	232, 1408, 1429, 1645, 1702, 1831
DraIII	CACNNNGTG	1 cut(s)	1691
DrdI	GACNNNNNNGTC	1 cut(s)	1218
DseDI	GACNNNNNNGTC	1 cut(s)	1218
EaeI	YGGCCR	1 cut(s)	469
Eam1104I	CTCTTC	4 cut(s)	125, 263, 554, 616
EarI	CTCTTC	4 cut(s)	125, 263, 554, 616
Eco130I	CCWWGG	1 cut(s)	1323
Eco24I	GRGCYC	1 cut(s)	1561
Eco32I	GATATC	3 cut(s)	943, 1620, 1823
Eco47I	GGWCC	6 cut(s)	437, 533, 1058, 1190, 1305, 2159
Eco47III	AGCGCT	1 cut(s)	2044
Eco57I	CTGAAG	4 cut(s)	879, 1030, 1503, 1787
Eco91I	GGTNACC	1 cut(s)	1165
EcoO109I	RGGNCCY	1 cut(s)	1305
EcoO65I	GGTNACC	1 cut(s)	1165
EcoRI	GAATTC	1 cut(s)	640
EcoRII	CCWGG	7 cut(s)	28, 44, 204, 1015, 1105, 1544, 1869
EcoRV	GATATC	3 cut(s)	943, 1620, 1823
EcoT14I	CCWWGG	1 cut(s)	1323
EcoT22I	ATGCAT	3 cut(s)	118, 424, 1755
EcoT38I	GRGCYC	1 cut(s)	1561
ErhI	CCWWGG	1 cut(s)	1323
FalI	AAGNNNNNCTT	2 cut(s)	1716, 1748
FaqI	GGGAC	1 cut(s)	94
Fnu4HI	GCNGC	7 cut(s)	309, 332, 996, 1038, 1246, 1355, 1850
FokI	GGATG	5 cut(s)	137, 188, 1487, 1616, 1961
FriOI	GRGCYC	1 cut(s)	1561
Fsp4HI	GCNGC	7 cut(s)	309, 332, 996, 1038, 1246, 1355, 1850
FspBI	CTAG	5 cut(s)	372, 500, 831, 1386, 1613
GlaI	GCGC	3 cut(s)	10, 90, 2044
GluI	GCNGC	7 cut(s)	309, 332, 996, 1038, 1246, 1355, 1850
GsuI	CTGGAG	2 cut(s)	1038, 1074
HaeII	RGCGCY	1 cut(s)	2046
HaeIII	GGCC	2 cut(s)	279, 471
HapII	CCGG	3 cut(s)	78, 483, 1258
HgaI	GACGC	2 cut(s)	765, 995
HhaI	GCGC	3 cut(s)	11, 91, 2045
Hin1I	GRCGYC	2 cut(s)	776, 1006
Hin6I	GCGC	3 cut(s)	9, 89, 2043
HinP1I	GCGC	3 cut(s)	9, 89, 2043
HindIII	AAGCTT	4 cut(s)	188, 245, 818, 891
HpaII	CCGG	3 cut(s)	78, 483, 1258
HphI	GGTGA	4 cut(s)	707, 1134, 1311, 2039
Hpy166II	GTNNAC	6 cut(s)	431, 566, 847, 971, 1502, 2134
Hpy188I	TCNGA	8 cut(s)	637, 781, 898, 1224, 1378, 1624, 1714, 1767
Hpy188III	TCNNGA	7 cut(s)	585, 793, 960, 1386, 1472, 1700, 1937
Hpy8I	GTNNAC	6 cut(s)	431, 566, 847, 971, 1502, 2134
HpyAV	CCTTC	9 cut(s)	115, 356, 610, 656, 766, 1143, 1277, 1742, 1927
HpyCH4III	ACNGT	2 cut(s)	1710, 2087
HpyF10VI	GCNNNNNNNGC	9 cut(s)	113, 419, 690, 995, 1351, 1691, 1849, 1855, 2151
HpyF3I	CTNAG	6 cut(s)	356, 636, 1419, 1511, 1560, 1942
Hsp92I	GRCGYC	2 cut(s)	776, 1006
HspAI	GCGC	3 cut(s)	9, 89, 2043
Kzo9I	GATC	6 cut(s)	232, 1408, 1429, 1645, 1702, 1831
LmnI	GCTCC	3 cut(s)	16, 1449, 2118
Lsp1109I	GCAGC	7 cut(s)	295, 318, 982, 1024, 1232, 1341, 1836
LweI	GCATC	5 cut(s)	409, 1369, 1594, 1861, 2015
MaeI	CTAG	5 cut(s)	372, 500, 831, 1386, 1613
MaeIII	GTNAC	3 cut(s)	1165, 1317, 1362
MalI	GATC	6 cut(s)	234, 1410, 1431, 1647, 1704, 1833
MbiI	CCGCTC	1 cut(s)	22
MboI	GATC	6 cut(s)	232, 1408, 1429, 1645, 1702, 1831
MfeI	CAATTG	1 cut(s)	1296
MflI	RGATCY	2 cut(s)	1429, 1831
MhlI	GDGCHC	2 cut(s)	205, 1561
MlsI	TGGCCA	1 cut(s)	471
MluCI	AATT	9 cut(s)	588, 640, 758, 806, 1000, 1137, 1216, 1296, 1781
MluNI	TGGCCA	1 cut(s)	471
MlyI	GAGTC	3 cut(s)	662, 862, 1934
MmeI	TCCRAC	3 cut(s)	423, 939, 1168
Mox20I	TGGCCA	1 cut(s)	471
Mph1103I	ATGCAT	3 cut(s)	118, 424, 1755
MroXI	GAANNNNTTC	3 cut(s)	625, 1731, 1916
MscI	TGGCCA	1 cut(s)	471
MseI	TTAA	6 cut(s)	518, 813, 909, 1011, 1742, 2066
MslI	CAYNNNNRTG	7 cut(s)	932, 978, 1377, 1440, 1580, 1602, 1857
Msp20I	TGGCCA	1 cut(s)	471
MspA1I	CMGCKG	1 cut(s)	1849
MspI	CCGG	3 cut(s)	78, 483, 1258
MspR9I	CCNGG	9 cut(s)	30, 46, 79, 206, 1017, 1107, 1259, 1546, 1871
MunI	CAATTG	1 cut(s)	1296
Mva1269I	GAATGC	2 cut(s)	118, 333
MvaI	CCWGG	7 cut(s)	30, 46, 206, 1017, 1107, 1546, 1871
MvnI	CGCG	1 cut(s)	259
MwoI	GCNNNNNNNGC	9 cut(s)	113, 419, 690, 995, 1351, 1691, 1849, 1855, 2151
NciI	CCSGG	2 cut(s)	79, 1259
NdeII	GATC	6 cut(s)	232, 1408, 1429, 1645, 1702, 1831
NlaIV	GGNNCC	4 cut(s)	1192, 1307, 1431, 1833
NmeAIII	GCCGAG	1 cut(s)	928
NmuCI	GTSAC	2 cut(s)	1317, 1362
NsiI	ATGCAT	3 cut(s)	118, 424, 1755
NspI	RCATGY	4 cut(s)	1184, 1673, 1862, 2008
NspV	TTCGAA	3 cut(s)	557, 614, 1281
OliI	CACNNNNGTG	1 cut(s)	932
PasI	CCCWGGG	1 cut(s)	29
PciI	ACATGT	1 cut(s)	1180
PctI	GAATGC	2 cut(s)	118, 333
PdmI	GAANNNNTTC	3 cut(s)	625, 1731, 1916
PfeI	GAWTC	7 cut(s)	375, 548, 572, 713, 1487, 1796, 1880
PflFI	GACNNNGTC	1 cut(s)	435
PfoI	TCCNGGA	1 cut(s)	1257
PkrI	GCNGC	7 cut(s)	310, 333, 997, 1039, 1247, 1356, 1851
PleI	GAGTC	3 cut(s)	661, 861, 1934
PpsI	GAGTC	3 cut(s)	661, 861, 1934
PpuMI	RGGWCCY	1 cut(s)	1305
PscI	ACATGT	1 cut(s)	1180
PshAI	GACNNNNGTC	1 cut(s)	852
Psp5II	RGGWCCY	1 cut(s)	1305
Psp6I	CCWGG	7 cut(s)	28, 44, 204, 1015, 1105, 1544, 1869
PspEI	GGTNACC	1 cut(s)	1165
PspGI	CCWGG	7 cut(s)	28, 44, 204, 1015, 1105, 1544, 1869
PspN4I	GGNNCC	4 cut(s)	1192, 1307, 1431, 1833
PspPI	GGNCC	7 cut(s)	277, 437, 533, 1058, 1190, 1305, 2159
PspPPI	RGGWCCY	1 cut(s)	1305
PstI	CTGCAG	2 cut(s)	336, 1347
PsuI	RGATCY	2 cut(s)	1429, 1831
PsyI	GACNNNGTC	1 cut(s)	435
PvuII	CAGCTG	1 cut(s)	1849
RsaI	GTAC	2 cut(s)	495, 567
RsaNI	GTAC	2 cut(s)	494, 566
RseI	CAYNNNNRTG	7 cut(s)	932, 978, 1377, 1440, 1580, 1602, 1857
SaqAI	TTAA	6 cut(s)	518, 813, 909, 1011, 1742, 2066
SatI	GCNGC	7 cut(s)	309, 332, 996, 1038, 1246, 1355, 1850
Sau3AI	GATC	6 cut(s)	232, 1408, 1429, 1645, 1702, 1831
Sau96I	GGNCC	7 cut(s)	277, 437, 533, 1058, 1190, 1305, 2159
SbfI	CCTGCAGG	1 cut(s)	1347
SchI	GAGTC	3 cut(s)	662, 862, 1934
ScrFI	CCNGG	4 cut(s)	30, 46, 79, 206
SdaI	CCTGCAGG	1 cut(s)	1347
SduI	GDGCHC	2 cut(s)	205, 1561
SfaNI	GCATC	5 cut(s)	409, 1369, 1594, 1861, 2015
SfcI	CTRYAG	3 cut(s)	332, 1234, 1343
SfuI	TTCGAA	3 cut(s)	557, 614, 1281
SgrAI	CRCCGGYG	1 cut(s)	482
SinI	GGWCC	6 cut(s)	437, 533, 1058, 1190, 1305, 2159
SmiMI	CAYNNNNRTG	7 cut(s)	932, 978, 1377, 1440, 1580, 1602, 1857
SmlI	CTYRAG	1 cut(s)	850
SmoI	CTYRAG	1 cut(s)	850
Sse8387I	CCTGCAGG	1 cut(s)	1347
Sse9I	AATT	9 cut(s)	588, 640, 758, 806, 1000, 1137, 1216, 1296, 1781
SsiI	CCGC	3 cut(s)	20, 259, 693
SspMI	CTAG	5 cut(s)	372, 500, 831, 1386, 1613
StyD4I	CCNGG	9 cut(s)	28, 44, 77, 204, 1015, 1105, 1257, 1544, 1869
StyI	CCWWGG	1 cut(s)	1323
TaaI	ACNGT	2 cut(s)	1710, 2087
TasI	AATT	9 cut(s)	588, 640, 758, 806, 1000, 1137, 1216, 1296, 1781
TatI	WGTACW	1 cut(s)	493
TfiI	GAWTC	7 cut(s)	375, 548, 572, 713, 1487, 1796, 1880
Tru1I	TTAA	6 cut(s)	518, 813, 909, 1011, 1742, 2066
Tru9I	TTAA	6 cut(s)	518, 813, 909, 1011, 1742, 2066
TscAI	CASTG	8 cut(s)	98, 463, 980, 1054, 1501, 1582, 1614, 1950
TseFI	GTSAC	2 cut(s)	1317, 1362
TseI	GCWGC	7 cut(s)	308, 331, 995, 1037, 1245, 1354, 1849
Tsp45I	GTSAC	2 cut(s)	1317, 1362
TspDTI	ATGAA	8 cut(s)	125, 206, 618, 944, 1214, 1709, 1770, 1788
TspGWI	ACGGA	3 cut(s)	429, 1802, 2151
TspRI	CASTG	8 cut(s)	98, 463, 980, 1054, 1501, 1582, 1614, 1950
Tth111I	GACNNNGTC	1 cut(s)	435
VpaK11BI	GGWCC	6 cut(s)	437, 533, 1058, 1190, 1305, 2159
XapI	RAATTY	3 cut(s)	640, 806, 1781
XbaI	TCTAGA	1 cut(s)	1385
XceI	RCATGY	4 cut(s)	1184, 1673, 1862, 2008
XmnI	GAANNNNTTC	3 cut(s)	625, 1731, 1916
XspI	CTAG	5 cut(s)	372, 500, 831, 1386, 1613
Zsp2I	ATGCAT	3 cut(s)	118, 424, 1755

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.