pycom05g05080

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002209
Physical Location & Seq
Forward (+)
6025260 .. 6028988
3729 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1023 bp
ATGGCGGAAAAAAGCGGCGACGGCGACGGCAGCGGTGGGGGTGATGGTGCTCCAGCAGAGGATGAGAAGAAGAATAATCATGCTGATGGTGATGTTCCACCAGAGAAAGAAAAGAATGGAGATCCAGAACTGCTGATCTGCTTGCTTCAACCTGCTCCCGTCGACTCCGATCCTGACTACATCGGCATCCGTCGCCTTCTCCTGTCTCGCAAGCCCCAGTCCCCATTTCATCGCCGCCTGGATTGGAGATGCAATGGAAAAGGGTACGTCGCGTTTCGAAATTACATCTGTCGGCCAAGGAATTGGGAGAGTACGCAGACTCCGAGCCTCCAGACCACTCCCGGAAACAGTGAGCGATGGATTCCACCTCCAAGTCCGCGGTCCCTTTTGTATGATGTCGAAAGCTGGAGTCCTGGCAGGGACGTCCAGAGTGGTAGTCAACCTTCACCTCGCAGAAGTTTTGGCTCCAGTATAAGTGATAGTGACCGCCCACGTCCTCAGCGGGCAGAACCTGCATACTCGTTCGTAGGAATGCAGTGCATCTTTGATCAGTGCAAAGCCTCTGTCACGGTTTTGAAGTTTGGACACATGAGTTCTGATCTGCTTGCCTACGGAGCATCAGATGGAACCTTGACAGTATGCACTGTTTCCGATCCACCTTCAATCCTCAAGCATCTGCATGGTCACTCCAAAGATGTTACAGACTTCGATTTTTCATCAAACAATCAATACATTTCATCCTCATCAATGGATAAAACTGTACGAGTGTGGGAGATTTCAAAAGGCCTTTGCATTCGAGTAATATATGGAGTGTCTCCACAACTATGTATTCGTTTCCACCCTGTAAATAACAACTTTCTTTCAGTTGGCAATGCAGACAAAGAAGTTACGGTTTTCAATTTCAGCACTGGGAGGATCATTCATAAAATATTCTTTGACAGTGAGGTTACCTCCATGGATTATGCTCACACTGGACAGCTCATTTTCTGTGGTGATGCGACGGTGGGTGTATATATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

341

Amino Acids

37.55

Weight (kDa)

6.14

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 179 - 270 1.7e-13 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 181 - 328 6.2e-07 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 183 - 262 3e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 184 - 330 3.8e-21 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 184 - 269 4.5e-11 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 185 - 302 1.9e-12 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 186 - 334 1.8e-14 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 188 - 328 9.8e-14 CDC20/Fizzy WD40 domain
WDR55 PF24796 196 - 260 9.9e-06 WDR55
Beta-prop_THOC3 PF25174 198 - 334 2.7e-19 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 222 - 311 3.4e-09 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 223 - 257 4.5e-10 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 232 - 332 6.4e-08 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 426
Acc36I ACCTGC 2 cut(s) 160, 520
AccI GTMKAC 1 cut(s) 162
AccII CGCG 2 cut(s) 272, 379
AciI CCGC 8 cut(s) 5, 15, 33, 235, 377, 379, 487, 502
AclWI GGATC 4 cut(s) 116, 164, 647, 923
AcoI YGGCCR 1 cut(s) 293
AcyI GRCGYC 1 cut(s) 423
AfaI GTAC 3 cut(s) 266, 313, 762
AgsI TTSAA 5 cut(s) 149, 577, 663, 780, 898
AjiI CACGTC 1 cut(s) 494
AjnI CCWGG 2 cut(s) 237, 412
AluBI AGCT 2 cut(s) 405, 979
AluI AGCT 2 cut(s) 405, 979
Alw21I GWGCWC 1 cut(s) 52
Alw26I GTCTC 2 cut(s) 210, 819
AlwI GGATC 4 cut(s) 116, 164, 647, 923
AlwNI CAGNNNCTG 1 cut(s) 512
AoxI GGCC 2 cut(s) 293, 784
ApeKI GCWGC 1 cut(s) 30
ArsI GACNNNNNNTTYG 2 cut(s) 549, 581
AspS9I GGNCC 1 cut(s) 381
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 4 cut(s) 53, 101, 438, 1004
AsuII TTCGAA 1 cut(s) 277
AvaII GGWCC 1 cut(s) 381
BarI GAAGNNNNNNTAC 2 cut(s) 427, 459
Bbv12I GWGCWC 1 cut(s) 52
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 1 cut(s) 42
BccI CCATC 4 cut(s) 38, 80, 351, 617
BceAI ACGGC 2 cut(s) 37, 43
BciT130I CCWGG 2 cut(s) 239, 414
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 1 cut(s) 342
BcoDI GTCTC 2 cut(s) 210, 819
BfuAI ACCTGC 2 cut(s) 160, 520
BisI GCNGC 3 cut(s) 16, 31, 235
BlsI GCNGC 3 cut(s) 17, 32, 236
Bme1390I CCNGG 3 cut(s) 239, 342, 414
Bme18I GGWCC 1 cut(s) 381
BmgBI CACGTC 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 381
BmiI GGNNCC 3 cut(s) 383, 466, 628
BmrFI CCNGG 3 cut(s) 239, 342, 414
BmrI ACTGGG 2 cut(s) 211, 918
BmsI GCATC 6 cut(s) 195, 239, 549, 626, 682, 985
BmuI ACTGGG 2 cut(s) 211, 918
BplI GAGNNNNNCTC 2 cut(s) 935, 967
BpmI CTGGAG 4 cut(s) 36, 314, 427, 451
Bpu10I CCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 1 cut(s) 277
BpuEI CTTGAG 1 cut(s) 653
BpuMI CCSGG 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 423
BsaJI CCNNGG 3 cut(s) 296, 377, 954
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bse1I ACTGG 4 cut(s) 217, 468, 913, 976
Bse3DI GCAATG 2 cut(s) 259, 877
BseBI CCWGG 2 cut(s) 239, 414
BseDI CCNNGG 3 cut(s) 296, 377, 954
BseGI GGATG 3 cut(s) 67, 186, 737
BseMI GCAATG 2 cut(s) 259, 877
BseMII CTCAG 1 cut(s) 512
BseNI ACTGG 4 cut(s) 217, 468, 913, 976
BseXI GCAGC 1 cut(s) 42
Bsh1236I CGCG 2 cut(s) 272, 379
BshFI GGCC 2 cut(s) 295, 786
BsiHKAI GWGCWC 1 cut(s) 52
BsiSI CCGG 1 cut(s) 342
BslFI GGGAC 3 cut(s) 205, 367, 434
BsmAI GTCTC 2 cut(s) 210, 819
BsmFI GGGAC 3 cut(s) 205, 367, 434
BsmI GAATGC 2 cut(s) 537, 792
BsnI GGCC 2 cut(s) 295, 786
Bsp119I TTCGAA 1 cut(s) 277
Bsp1286I GDGCHC 1 cut(s) 52
Bsp143I GATC 7 cut(s) 121, 135, 169, 547, 598, 652, 915
Bsp19I CCATGG 1 cut(s) 954
BspACI CCGC 8 cut(s) 5, 15, 33, 235, 377, 379, 487, 502
BspANI GGCC 2 cut(s) 295, 786
BspCNI CTCAG 1 cut(s) 511
BspFNI CGCG 2 cut(s) 272, 379
BspLI GGNNCC 3 cut(s) 383, 466, 628
BspMI ACCTGC 2 cut(s) 160, 520
BspPI GGATC 4 cut(s) 116, 164, 647, 923
BspT104I TTCGAA 1 cut(s) 277
BsrDI GCAATG 2 cut(s) 259, 877
BsrI ACTGG 4 cut(s) 217, 468, 913, 976
BssECI CCNNGG 3 cut(s) 296, 377, 954
BssMI GATC 7 cut(s) 121, 135, 169, 547, 598, 652, 915
BssNI GRCGYC 1 cut(s) 423
BssT1I CCWWGG 2 cut(s) 296, 954
Bst2UI CCWGG 2 cut(s) 239, 414
Bst4CI ACNGT 8 cut(s) 350, 571, 637, 646, 760, 892, 941, 1003
BstACI GRCGYC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 277
BstC8I GCNNGC 4 cut(s) 143, 212, 504, 606
BstDEI CTNAG 1 cut(s) 498
BstDSI CCRYGG 2 cut(s) 377, 954
BstEII GGTNACC 1 cut(s) 946
BstF5I GGATG 3 cut(s) 67, 186, 737
BstFNI CGCG 2 cut(s) 272, 379
BstKTI GATC 7 cut(s) 124, 138, 172, 550, 601, 655, 918
BstMAI GTCTC 2 cut(s) 210, 819
BstMBI GATC 7 cut(s) 121, 135, 169, 547, 598, 652, 915
BstMWI GCNNNNNNNGC 5 cut(s) 21, 30, 192, 512, 614
BstNI CCWGG 2 cut(s) 239, 414
BstPI GGTNACC 1 cut(s) 946
BstSCI CCNGG 3 cut(s) 237, 340, 412
BstUI CGCG 2 cut(s) 272, 379
BstV1I GCAGC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 303
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 2 cut(s) 295, 786
BtgI CCRYGG 2 cut(s) 377, 954
BtgZI GCGATG 2 cut(s) 215, 370
BtrI CACGTC 1 cut(s) 494
BtsCI GGATG 3 cut(s) 67, 186, 737
BtsI GCAGTG 1 cut(s) 542
BtsIMutI CAGTG 7 cut(s) 355, 542, 557, 642, 906, 946, 969
BveI ACCTGC 2 cut(s) 160, 520
Cac8I GCNNGC 4 cut(s) 143, 212, 504, 606
CaiI CAGNNNCTG 1 cut(s) 512
Cfr13I GGNCC 1 cut(s) 381
Cfr42I CCGCGG 1 cut(s) 380
Csp6I GTAC 3 cut(s) 265, 312, 761
CviAII CATG 4 cut(s) 80, 589, 680, 955
CviJI RGCY 8 cut(s) 214, 295, 327, 405, 465, 560, 786, 979
CviKI_1 RGCY 8 cut(s) 214, 295, 327, 405, 465, 560, 786, 979
CviQI GTAC 3 cut(s) 265, 312, 761
DdeI CTNAG 1 cut(s) 498
DpnI GATC 7 cut(s) 123, 137, 171, 549, 600, 654, 917
DpnII GATC 7 cut(s) 121, 135, 169, 547, 598, 652, 915
EaeI YGGCCR 1 cut(s) 293
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 296, 954
Eco147I AGGCCT 1 cut(s) 786
Eco47I GGWCC 1 cut(s) 381
Eco91I GGTNACC 1 cut(s) 946
EcoO65I GGTNACC 1 cut(s) 946
EcoRII CCWGG 2 cut(s) 237, 412
EcoT14I CCWWGG 2 cut(s) 296, 954
ErhI CCWWGG 2 cut(s) 296, 954
FaeI CATG 4 cut(s) 83, 592, 683, 958
FaqI GGGAC 3 cut(s) 205, 367, 434
FatI CATG 4 cut(s) 79, 588, 679, 954
FauI CCCGC 1 cut(s) 495
FbaI TGATCA 1 cut(s) 547
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 16, 31, 235
FokI GGATG 3 cut(s) 74, 173, 724
Fsp4HI GCNGC 3 cut(s) 16, 31, 235
GluI GCNGC 3 cut(s) 16, 31, 235
GsuI CTGGAG 4 cut(s) 36, 314, 427, 451
HaeIII GGCC 2 cut(s) 295, 786
HapII CCGG 1 cut(s) 342
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 4 cut(s) 83, 592, 683, 958
HincII GTYRAC 2 cut(s) 163, 440
HindII GTYRAC 2 cut(s) 163, 440
HinfI GANTC 4 cut(s) 164, 319, 361, 409
HpaII CCGG 1 cut(s) 342
HphI GGTGA 4 cut(s) 53, 101, 438, 1004
Hpy166II GTNNAC 2 cut(s) 163, 440
Hpy188I TCNGA 5 cut(s) 169, 324, 598, 622, 652
Hpy188III TCNNGA 4 cut(s) 125, 173, 331, 427
Hpy8I GTNNAC 2 cut(s) 163, 440
Hpy99I CGWCG 6 cut(s) 23, 29, 164, 195, 272, 1003
HpyAV CCTTC 3 cut(s) 206, 453, 669
HpyCH4III ACNGT 8 cut(s) 350, 571, 637, 646, 760, 892, 941, 1003
HpyCH4IV ACGT 3 cut(s) 267, 423, 493
HpyCH4V TGCA 9 cut(s) 252, 515, 535, 540, 555, 642, 679, 792, 875
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 30, 192, 512, 614
HpyF3I CTNAG 1 cut(s) 498
HpySE526I ACGT 3 cut(s) 267, 423, 493
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 4 cut(s) 83, 592, 683, 958
Ksp22I TGATCA 1 cut(s) 547
KspI CCGCGG 1 cut(s) 380
Kzo9I GATC 7 cut(s) 121, 135, 169, 547, 598, 652, 915
LmnI GCTCC 4 cut(s) 55, 160, 470, 614
Lsp1109I GCAGC 1 cut(s) 42
LweI GCATC 6 cut(s) 195, 239, 549, 626, 682, 985
MaeII ACGT 3 cut(s) 267, 423, 493
MaeIII GTNAC 6 cut(s) 482, 565, 683, 697, 886, 946
MalI GATC 7 cut(s) 123, 137, 171, 549, 600, 654, 917
MboI GATC 7 cut(s) 121, 135, 169, 547, 598, 652, 915
MboII GAAGA 2 cut(s) 79, 82
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 3 cut(s) 280, 301, 898
MlyI GAGTC 3 cut(s) 158, 313, 418
MslI CAYNNNNRTG 3 cut(s) 84, 678, 823
MspA1I CMGCKG 3 cut(s) 33, 379, 502
MspI CCGG 1 cut(s) 342
MspR9I CCNGG 3 cut(s) 239, 342, 414
Mva1269I GAATGC 2 cut(s) 537, 792
MvaI CCWGG 2 cut(s) 239, 414
MvnI CGCG 2 cut(s) 272, 379
MwoI GCNNNNNNNGC 5 cut(s) 21, 30, 192, 512, 614
NciI CCSGG 1 cut(s) 342
NcoI CCATGG 1 cut(s) 954
NdeII GATC 7 cut(s) 121, 135, 169, 547, 598, 652, 915
NlaIII CATG 4 cut(s) 83, 592, 683, 958
NlaIV GGNNCC 3 cut(s) 383, 466, 628
NmuCI GTSAC 3 cut(s) 482, 565, 683
NspV TTCGAA 1 cut(s) 277
PceI AGGCCT 1 cut(s) 786
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PctI GAATGC 2 cut(s) 537, 792
PfeI GAWTC 1 cut(s) 361
PfoI TCCNGGA 1 cut(s) 340
PkrI GCNGC 3 cut(s) 17, 32, 236
PleI GAGTC 3 cut(s) 158, 313, 417
PpsI GAGTC 3 cut(s) 158, 313, 417
Psp6I CCWGG 2 cut(s) 237, 412
PspEI GGTNACC 1 cut(s) 946
PspGI CCWGG 2 cut(s) 237, 412
PspN4I GGNNCC 3 cut(s) 383, 466, 628
PspPI GGNCC 1 cut(s) 381
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 3 cut(s) 266, 313, 762
RsaNI GTAC 3 cut(s) 265, 312, 761
RseI CAYNNNNRTG 3 cut(s) 84, 678, 823
SacII CCGCGG 1 cut(s) 380
SalI GTCGAC 1 cut(s) 161
SatI GCNGC 3 cut(s) 16, 31, 235
Sau3AI GATC 7 cut(s) 121, 135, 169, 547, 598, 652, 915
Sau96I GGNCC 1 cut(s) 381
SchI GAGTC 3 cut(s) 158, 313, 418
ScrFI CCNGG 3 cut(s) 239, 342, 414
SduI GDGCHC 1 cut(s) 52
SfaNI GCATC 6 cut(s) 195, 239, 549, 626, 682, 985
Sfr303I CCGCGG 1 cut(s) 380
SfuI TTCGAA 1 cut(s) 277
SgrBI CCGCGG 1 cut(s) 380
SinI GGWCC 1 cut(s) 381
SmiMI CAYNNNNRTG 3 cut(s) 84, 678, 823
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
Sse9I AATT 3 cut(s) 280, 301, 898
SseBI AGGCCT 1 cut(s) 786
SsiI CCGC 8 cut(s) 5, 15, 33, 235, 377, 379, 487, 502
SspI AATATT 1 cut(s) 930
StuI AGGCCT 1 cut(s) 786
StyD4I CCNGG 3 cut(s) 237, 340, 412
StyI CCWWGG 2 cut(s) 296, 954
TaaI ACNGT 8 cut(s) 350, 571, 637, 646, 760, 892, 941, 1003
TaiI ACGT 3 cut(s) 270, 426, 496
TaqI TCGA 5 cut(s) 162, 277, 399, 708, 796
TasI AATT 3 cut(s) 280, 301, 898
TauI GCSGC 2 cut(s) 18, 237
TfiI GAWTC 1 cut(s) 361
TscAI CASTG 7 cut(s) 355, 542, 557, 649, 913, 946, 976
TseFI GTSAC 3 cut(s) 482, 565, 683
TseI GCWGC 1 cut(s) 30
Tsp45I GTSAC 3 cut(s) 482, 565, 683
TspDTI ATGAA 4 cut(s) 218, 705, 726, 911
TspGWI ACGGA 2 cut(s) 179, 627
TspRI CASTG 7 cut(s) 355, 542, 557, 649, 913, 946, 976
VpaK11BI GGWCC 1 cut(s) 381
XmiI GTMKAC 1 cut(s) 162
ZraI GACGTC 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.