pycom05g17880

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003544
Physical Location & Seq
Reverse (-)
20999087 .. 21000917
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17880.1

Sequence Viewer

Length: 1302 bp
ATGGGTTGCTTTCTTGCATGTTTTGGTTCTTCCAAAGACAAAAAACACAGGAGTAATCAGAGGTACAGGGTTCACCACAGAGACCATAGACATACAAGTTTCGAGCCTGTGAAATCTGCCGTCTCTTCTGCACCGGTGGTTGAGGAAAAACCTATTATCCCGGCATTGGAAGAAGTCCGGGATAAGCCAGTGGAGGAACTGAGCTTCAGTACTCGAAAGAAAGTTACTTTTGATTCGAATGTCAAGACCTATGAGCATGTTTCGACCAACGAAACCCCGGATCCTTTGTTGGATAGTGAAGAGAATGGGAAGGAGGAAGAGGAGGGGAAGAATTTGGAAAAACCGTGCCAATCTAAGTCTTCCTCTGAAGATAGTTCGGTCACTTCGAGCTCAGGGTCATATCCTCCTAATCATAGGTACCAGAATTGCAGGGATAGCGATGATGAAGACGAAGCGTTGGACTATGATGAGGACTGTGATCTTGATGGTGAAGATGAGGATGATTACGATGACGATGATGGGGAACTAGAGTACGAAGATGAAATTGTAGAATCAAACGGAGGGGTTTCTACACGTCAAGTAATGACTGAGGATTTTGATAGTTCAATGCCGATGAAACCAGCTGGGTTGAACCACAATGCGCGCGATAGGAGTGGTTATGTTCATTCGGTGCTAAACCCTGTTGAGAATCTTACACAATGGAAAGCTCTCAAAGCCAAAGGGACACCACTGATGAAACCTCAGAAAGAGAATTTCACACAGGATCAAGAACCCCGGATTTCATTCAGTTCAGAGCCAAGTTTGAACTTCAAATCGAAAGCCGATCAAGACAAGAAGCATTCAAAGAATTCCGACCGAGAAATGGCAGTTGATGCTAGCCTTTCGAACTGGTTGTTTTCATCAGAAAATACACCGGTTAACAAGGCCAGCACAACTGCTTTAGACACCTTCACACCTGAGAAAAGCACGTCCCATGGATCAAACTCAGCGAGAAGCCATGAAGATCGACCAATTTTAGGTGCATTAACTGTTGAAGAGCTCAGACAGTTTTCGGCTTCATCTTCACCAAGGAAATCACCGAGTAGAAGCCCTGACGAGATGGCCATAATGGGGACTGTCGGAACCTACTGGAATCACATGGCTCCTCCTCCCGCTGCCAAGAGTTCGGGTGCCTCGTCTTACAAAGGAATACCAAACACAACCAGCAAATACAGAGAGGATAAAAGAGTGAATTGGCATTCTACCCCTTTCGAGACGAGGTTGGAAAGAGCTTTGAACAGAGGAGGTGCTGCTGAACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

434

Amino Acids

48.4

Weight (kDa)

5.07

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 417
AccB1I GGYRCC 2 cut(s) 417, 1169
AccII CGCG 2 cut(s) 643, 645
AciI CCGC 1 cut(s) 1152
AclWI GGATC 4 cut(s) 275, 288, 771, 985
AcoI YGGCCR 1 cut(s) 1101
AcsI RAATTY 3 cut(s) 331, 751, 847
AcuI CTGAAG 2 cut(s) 190, 387
AfaI GTAC 4 cut(s) 65, 211, 419, 533
AfiI CCNNNNNNNGG 4 cut(s) 166, 862, 1016, 1110
AflIII ACRYGT 1 cut(s) 572
AgeI ACCGGT 2 cut(s) 133, 913
AgsI TTSAA 7 cut(s) 606, 631, 805, 811, 843, 1034, 1276
AjiI CACGTC 2 cut(s) 575, 969
AluBI AGCT 6 cut(s) 204, 390, 623, 707, 1039, 1271
AluI AGCT 6 cut(s) 204, 390, 623, 707, 1039, 1271
Alw21I GWGCWC 2 cut(s) 392, 1041
Alw26I GTCTC 3 cut(s) 75, 127, 1247
AlwI GGATC 4 cut(s) 275, 288, 771, 985
AoxI GGCC 2 cut(s) 924, 1101
ApeKI GCWGC 2 cut(s) 1154, 1289
ApoI RAATTY 3 cut(s) 331, 751, 847
AsiGI ACCGGT 2 cut(s) 133, 913
Asp718I GGTACC 1 cut(s) 417
AspLEI GCGC 2 cut(s) 643, 645
AsuC2I CCSGG 4 cut(s) 161, 179, 278, 775
AsuHPI GGTGA 4 cut(s) 65, 500, 1056, 1068
AsuII TTCGAA 2 cut(s) 236, 884
AsuNHI GCTAGC 1 cut(s) 875
BalI TGGCCA 1 cut(s) 1103
BamHI GGATCC 1 cut(s) 280
BanI GGYRCC 2 cut(s) 417, 1169
BanII GRGCYC 2 cut(s) 392, 1041
BbsI GAAGAC 2 cut(s) 351, 453
Bbv12I GWGCWC 2 cut(s) 392, 1041
BbvI GCAGC 2 cut(s) 1141, 1276
BccI CCATC 3 cut(s) 479, 512, 1093
BceAI ACGGC 1 cut(s) 104
BcnI CCSGG 4 cut(s) 161, 179, 278, 775
BcoDI GTCTC 3 cut(s) 75, 127, 1247
BfaI CTAG 2 cut(s) 527, 876
BisI GCNGC 2 cut(s) 1155, 1290
BlsI GCNGC 2 cut(s) 1156, 1291
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 4 cut(s) 161, 179, 278, 775
BmgBI CACGTC 2 cut(s) 575, 969
BmiI GGNNCC 5 cut(s) 282, 419, 1123, 1143, 1171
BmrFI CCNGG 4 cut(s) 161, 179, 278, 775
BmsI GCATC 1 cut(s) 862
BmtI GCTAGC 1 cut(s) 879
BpiI GAAGAC 2 cut(s) 351, 453
Bpu10I CCTNAGC 1 cut(s) 391
Bpu14I TTCGAA 2 cut(s) 236, 884
BpuMI CCSGG 4 cut(s) 161, 179, 278, 775
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 4 cut(s) 276, 773, 973, 1067
BsaWI WCCGGW 2 cut(s) 133, 913
Bsc4I CCNNNNNNNGG 4 cut(s) 166, 862, 1016, 1110
Bse118I RCCGGY 2 cut(s) 133, 913
Bse1I ACTGG 3 cut(s) 188, 893, 1133
BseDI CCNNGG 4 cut(s) 276, 773, 973, 1067
BseGI GGATG 1 cut(s) 505
BseLI CCNNNNNNNGG 4 cut(s) 166, 862, 1016, 1110
BseMII CTCAG 7 cut(s) 191, 405, 579, 755, 948, 999, 1054
BseNI ACTGG 3 cut(s) 188, 893, 1133
BsePI GCGCGC 1 cut(s) 641
BseRI GAGGAG 4 cut(s) 335, 1134, 1137, 1296
BseXI GCAGC 2 cut(s) 1141, 1276
BseYI CCCAGC 1 cut(s) 623
BsgI GTGCAG 1 cut(s) 114
Bsh1236I CGCG 2 cut(s) 643, 645
Bsh1285I CGRYCG 1 cut(s) 856
BshFI GGCC 2 cut(s) 926, 1103
BshNI GGYRCC 2 cut(s) 417, 1169
BshTI ACCGGT 2 cut(s) 133, 913
BsiEI CGRYCG 1 cut(s) 856
BsiHKAI GWGCWC 2 cut(s) 392, 1041
BsiSI CCGG 6 cut(s) 134, 161, 178, 278, 775, 914
BslFI GGGAC 3 cut(s) 736, 955, 1126
BslI CCNNNNNNNGG 4 cut(s) 166, 862, 1016, 1110
BsmAI GTCTC 3 cut(s) 75, 127, 1247
BsmBI CGTCTC 2 cut(s) 127, 1247
BsmFI GGGAC 3 cut(s) 736, 955, 1126
BsmI GAATGC 2 cut(s) 838, 1237
BsnI GGCC 2 cut(s) 926, 1103
Bso31I GGTCTC 1 cut(s) 75
Bsp119I TTCGAA 2 cut(s) 236, 884
Bsp1286I GDGCHC 2 cut(s) 392, 1041
Bsp143I GATC 6 cut(s) 280, 478, 763, 823, 977, 1003
Bsp19I CCATGG 1 cut(s) 973
BspACI CCGC 1 cut(s) 1152
BspANI GGCC 2 cut(s) 926, 1103
BspCNI CTCAG 7 cut(s) 192, 404, 580, 754, 949, 998, 1053
BspFNI CGCG 2 cut(s) 643, 645
BspLI GGNNCC 5 cut(s) 282, 419, 1123, 1143, 1171
BspOI GCTAGC 1 cut(s) 879
BspPI GGATC 4 cut(s) 275, 288, 771, 985
BspQI GCTCTTC 1 cut(s) 1029
BspT104I TTCGAA 2 cut(s) 236, 884
BspT107I GGYRCC 2 cut(s) 417, 1169
BspTNI GGTCTC 1 cut(s) 75
BsrFI RCCGGY 2 cut(s) 133, 913
BsrI ACTGG 3 cut(s) 188, 893, 1133
BssAI RCCGGY 2 cut(s) 133, 913
BssECI CCNNGG 4 cut(s) 276, 773, 973, 1067
BssHII GCGCGC 1 cut(s) 641
BssMI GATC 6 cut(s) 280, 478, 763, 823, 977, 1003
BssT1I CCWWGG 2 cut(s) 973, 1067
Bst4CI ACNGT 5 cut(s) 345, 476, 1030, 1047, 1117
Bst6I CTCTTC 4 cut(s) 130, 294, 312, 1029
BstAPI GCANNNNNTGC 1 cut(s) 872
BstBI TTCGAA 2 cut(s) 236, 884
BstC8I GCNNGC 3 cut(s) 643, 877, 928
BstDEI CTNAG 8 cut(s) 200, 354, 391, 588, 741, 957, 985, 1040
BstDSI CCRYGG 1 cut(s) 973
BstF5I GGATG 1 cut(s) 505
BstFNI CGCG 2 cut(s) 643, 645
BstHHI GCGC 2 cut(s) 643, 645
BstKTI GATC 6 cut(s) 283, 481, 766, 826, 980, 1006
BstMAI GTCTC 3 cut(s) 75, 127, 1247
BstMBI GATC 6 cut(s) 280, 478, 763, 823, 977, 1003
BstMCI CGRYCG 1 cut(s) 856
BstMWI GCNNNNNNNGC 3 cut(s) 435, 713, 872
BstNSI RCATGY 2 cut(s) 21, 260
BstSCI CCNGG 4 cut(s) 159, 177, 276, 773
BstUI CGCG 2 cut(s) 643, 645
BstV1I GCAGC 2 cut(s) 1141, 1276
BstV2I GAAGAC 2 cut(s) 351, 453
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BsuRI GGCC 2 cut(s) 926, 1103
BtgI CCRYGG 1 cut(s) 973
BtgZI GCGATG 1 cut(s) 453
BtrI CACGTC 2 cut(s) 575, 969
BtsCI GGATG 1 cut(s) 505
BtsIMutI CAGTG 2 cut(s) 195, 728
Cac8I GCNNGC 3 cut(s) 643, 877, 928
CfoI GCGC 2 cut(s) 643, 645
Cfr10I RCCGGY 2 cut(s) 133, 913
Csp6I GTAC 4 cut(s) 64, 210, 418, 532
CspAI ACCGGT 2 cut(s) 133, 913
CviAII CATG 5 cut(s) 18, 257, 974, 998, 1138
CviQI GTAC 4 cut(s) 64, 210, 418, 532
DdeI CTNAG 8 cut(s) 200, 354, 391, 588, 741, 957, 985, 1040
DpnI GATC 6 cut(s) 282, 480, 765, 825, 979, 1005
DpnII GATC 6 cut(s) 280, 478, 763, 823, 977, 1003
EaeI YGGCCR 1 cut(s) 1101
Eam1104I CTCTTC 4 cut(s) 130, 294, 312, 1029
EarI CTCTTC 4 cut(s) 130, 294, 312, 1029
Ecl136II GAGCTC 2 cut(s) 390, 1039
Eco130I CCWWGG 2 cut(s) 973, 1067
Eco24I GRGCYC 2 cut(s) 392, 1041
Eco31I GGTCTC 1 cut(s) 75
Eco53kI GAGCTC 2 cut(s) 390, 1039
Eco57I CTGAAG 2 cut(s) 190, 387
EcoICRI GAGCTC 2 cut(s) 390, 1039
EcoRI GAATTC 1 cut(s) 847
EcoT14I CCWWGG 2 cut(s) 973, 1067
EcoT38I GRGCYC 2 cut(s) 392, 1041
ErhI CCWWGG 2 cut(s) 973, 1067
Esp3I CGTCTC 2 cut(s) 127, 1247
FaeI CATG 5 cut(s) 21, 260, 977, 1001, 1141
FaqI GGGAC 3 cut(s) 736, 955, 1126
FatI CATG 5 cut(s) 17, 256, 973, 997, 1137
FauI CCCGC 1 cut(s) 1159
Fnu4HI GCNGC 2 cut(s) 1155, 1290
FokI GGATG 1 cut(s) 512
FriOI GRGCYC 2 cut(s) 392, 1041
Fsp4HI GCNGC 2 cut(s) 1155, 1290
FspBI CTAG 2 cut(s) 527, 876
GlaI GCGC 2 cut(s) 642, 644
GluI GCNGC 2 cut(s) 1155, 1290
GsaI CCCAGC 1 cut(s) 627
HaeIII GGCC 2 cut(s) 926, 1103
HapII CCGG 6 cut(s) 134, 161, 178, 278, 775, 914
HhaI GCGC 2 cut(s) 643, 645
Hin1II CATG 5 cut(s) 21, 260, 977, 1001, 1141
Hin6I GCGC 2 cut(s) 641, 643
HinP1I GCGC 2 cut(s) 641, 643
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HinfI GANTC 4 cut(s) 233, 551, 688, 1132
HpaI GTTAAC 1 cut(s) 919
HpaII CCGG 6 cut(s) 134, 161, 178, 278, 775, 914
HphI GGTGA 4 cut(s) 65, 500, 1056, 1068
Hpy166II GTNNAC 2 cut(s) 73, 919
Hpy188I TCNGA 8 cut(s) 60, 367, 744, 793, 853, 904, 1043, 1121
Hpy188III TCNNGA 5 cut(s) 244, 482, 767, 827, 1252
Hpy8I GTNNAC 2 cut(s) 73, 919
HpyAV CCTTC 2 cut(s) 304, 958
HpyCH4III ACNGT 5 cut(s) 345, 476, 1030, 1047, 1117
HpyCH4IV ACGT 2 cut(s) 574, 968
HpyCH4V TGCA 4 cut(s) 17, 131, 429, 1022
HpyF10VI GCNNNNNNNGC 3 cut(s) 435, 713, 872
HpyF3I CTNAG 8 cut(s) 200, 354, 391, 588, 741, 957, 985, 1040
HpySE526I ACGT 2 cut(s) 574, 968
Hsp92II CATG 5 cut(s) 21, 260, 977, 1001, 1141
HspAI GCGC 2 cut(s) 641, 643
KpnI GGTACC 1 cut(s) 421
KspAI GTTAAC 1 cut(s) 919
Kzo9I GATC 6 cut(s) 280, 478, 763, 823, 977, 1003
LguI GCTCTTC 1 cut(s) 1029
LmnI GCTCC 1 cut(s) 1147
Lsp1109I GCAGC 2 cut(s) 1141, 1276
LweI GCATC 1 cut(s) 862
MaeI CTAG 2 cut(s) 527, 876
MaeII ACGT 2 cut(s) 574, 968
MaeIII GTNAC 2 cut(s) 223, 379
MalI GATC 6 cut(s) 282, 480, 765, 825, 979, 1005
MboI GATC 6 cut(s) 280, 478, 763, 823, 977, 1003
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 2 cut(s) 392, 1041
MlsI TGGCCA 1 cut(s) 1103
MluCI AATT 7 cut(s) 331, 424, 543, 751, 847, 1011, 1231
MluNI TGGCCA 1 cut(s) 1103
MmeI TCCRAC 5 cut(s) 270, 438, 876, 1099, 1242
Mox20I TGGCCA 1 cut(s) 1103
MscI TGGCCA 1 cut(s) 1103
MseI TTAA 2 cut(s) 918, 1025
Msp20I TGGCCA 1 cut(s) 1103
MspA1I CMGCKG 2 cut(s) 623, 1154
MspI CCGG 6 cut(s) 134, 161, 178, 278, 775, 914
MspR9I CCNGG 4 cut(s) 161, 179, 278, 775
Mva1269I GAATGC 2 cut(s) 838, 1237
MvnI CGCG 2 cut(s) 643, 645
MwoI GCNNNNNNNGC 3 cut(s) 435, 713, 872
NciI CCSGG 4 cut(s) 161, 179, 278, 775
NcoI CCATGG 1 cut(s) 973
NdeII GATC 6 cut(s) 280, 478, 763, 823, 977, 1003
NheI GCTAGC 1 cut(s) 875
NlaIII CATG 5 cut(s) 21, 260, 977, 1001, 1141
NlaIV GGNNCC 5 cut(s) 282, 419, 1123, 1143, 1171
NmuCI GTSAC 1 cut(s) 379
NspI RCATGY 2 cut(s) 21, 260
NspV TTCGAA 2 cut(s) 236, 884
PauI GCGCGC 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 1029
PcsI WCGNNNNNNNCGW 2 cut(s) 383, 1172
PctI GAATGC 2 cut(s) 838, 1237
PfeI GAWTC 4 cut(s) 233, 551, 688, 1132
PfoI TCCNGGA 1 cut(s) 177
PinAI ACCGGT 2 cut(s) 133, 913
PkrI GCNGC 2 cut(s) 1156, 1291
Psp124BI GAGCTC 2 cut(s) 392, 1041
PspFI CCCAGC 1 cut(s) 623
PspN4I GGNNCC 5 cut(s) 282, 419, 1123, 1143, 1171
PsuI RGATCY 1 cut(s) 280
PteI GCGCGC 1 cut(s) 641
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 4 cut(s) 65, 211, 419, 533
RsaNI GTAC 4 cut(s) 64, 210, 418, 532
SacI GAGCTC 2 cut(s) 392, 1041
SapI GCTCTTC 1 cut(s) 1029
SaqAI TTAA 2 cut(s) 918, 1025
SatI GCNGC 2 cut(s) 1155, 1290
Sau3AI GATC 6 cut(s) 280, 478, 763, 823, 977, 1003
ScaI AGTACT 1 cut(s) 211
ScrFI CCNGG 4 cut(s) 161, 179, 278, 775
SduI GDGCHC 2 cut(s) 392, 1041
SfaNI GCATC 1 cut(s) 862
SfuI TTCGAA 2 cut(s) 236, 884
SgrAI CRCCGGYG 1 cut(s) 133
Sse9I AATT 7 cut(s) 331, 424, 543, 751, 847, 1011, 1231
SsiI CCGC 1 cut(s) 1152
SspMI CTAG 2 cut(s) 527, 876
SstI GAGCTC 2 cut(s) 392, 1041
StyD4I CCNGG 4 cut(s) 159, 177, 276, 773
StyI CCWWGG 2 cut(s) 973, 1067
TaaI ACNGT 5 cut(s) 345, 476, 1030, 1047, 1117
TaiI ACGT 2 cut(s) 577, 971
TaqI TCGA 9 cut(s) 102, 214, 236, 263, 386, 815, 884, 1006, 1251
TaqII GACCGA 2 cut(s) 367, 870
TasI AATT 7 cut(s) 331, 424, 543, 751, 847, 1011, 1231
TatI WGTACW 1 cut(s) 209
TfiI GAWTC 4 cut(s) 233, 551, 688, 1132
Tru1I TTAA 2 cut(s) 918, 1025
Tru9I TTAA 2 cut(s) 918, 1025
TscAI CASTG 2 cut(s) 195, 735
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 2 cut(s) 1154, 1289
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 9 cut(s) 459, 555, 629, 653, 749, 771, 888, 1014, 1047
TspGWI ACGGA 1 cut(s) 573
TspRI CASTG 2 cut(s) 195, 735
XapI RAATTY 3 cut(s) 331, 751, 847
XceI RCATGY 2 cut(s) 21, 260
XspI CTAG 2 cut(s) 527, 876
ZrmI AGTACT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.