Rh6DG294000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003544
Physical Location & Seq
Reverse (-)
49493735 .. 49494284
550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG294000.1

Sequence Viewer

Length: 465 bp
ATGGCAGTTGATGCTAGCCTGTCAAATTGTTTGGGTTCATCAGAAGCTACACTTGTTAACAAGACTAGCACAATATCTCTGGACACGTCTATGCCTGAGAAAAGCATGTCCTACGGATTACCACACTCAGCAACAAGCCACGAAGATAGACCAATTTTAGGTGCATTAACAGTTGAAGAGCTGAGACAGTTTTCAACTTCATCTTCACCGCGGAAGTCACCTAGCAGAAGCCCTGATGAGATGGCCATAATTGGGACTGTTGGGAGCTACTGGAATGATCACACTGGTCCTGCCAAGGGTTCTGGCGCCTCCTCTTACAAAGGAATATCAAACACGACCAGCAAGTACAGAGAGGATAGAAGAGTCAACTGGCACTCTACCCCATTTGAGACACGGCTGGAAAGAGCTTTGAATAGAGGTGTTGCAGATGCTTACTCTTCTTCTCGTACGCCTCATGGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

16.62

Weight (kDa)

7.92

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 305
AccII CGCG 1 cut(s) 211
AciI CCGC 2 cut(s) 209, 211
AcoI YGGCCR 1 cut(s) 243
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 2 cut(s) 347, 448
AfiI CCNNNNNNNGG 3 cut(s) 158, 252, 296
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 3 cut(s) 176, 195, 412
AjiI CACGTC 1 cut(s) 87
AluBI AGCT 4 cut(s) 47, 181, 267, 407
AluI AGCT 4 cut(s) 47, 181, 267, 407
Alw26I GTCTC 2 cut(s) 178, 383
AoxI GGCC 1 cut(s) 243
AspLEI GCGC 1 cut(s) 308
AspS9I GGNCC 1 cut(s) 287
AsuHPI GGTGA 2 cut(s) 198, 210
AsuNHI GCTAGC 1 cut(s) 14
AvaII GGWCC 1 cut(s) 287
BalI TGGCCA 1 cut(s) 245
BanI GGYRCC 1 cut(s) 305
BccI CCATC 1 cut(s) 235
BceAI ACGGC 1 cut(s) 410
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 2 cut(s) 178, 383
BfaI CTAG 3 cut(s) 15, 66, 222
BfoI RGCGCY 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 307
BmsI GCATC 1 cut(s) 418
BmtI GCTAGC 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 2 cut(s) 209, 294
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 252, 296
Bse1I ACTGG 3 cut(s) 275, 289, 374
BseDI CCNNGG 2 cut(s) 209, 294
BseLI CCNNNNNNNGG 3 cut(s) 158, 252, 296
BseMII CTCAG 3 cut(s) 87, 141, 173
BseNI ACTGG 3 cut(s) 275, 289, 374
BseRI GAGGAG 1 cut(s) 301
Bsh1236I CGCG 1 cut(s) 211
BshFI GGCC 1 cut(s) 245
BshNI GGYRCC 1 cut(s) 305
BsiWI CGTACG 1 cut(s) 446
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 3 cut(s) 158, 252, 296
BsmAI GTCTC 2 cut(s) 178, 383
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 1 cut(s) 245
Bsp143I GATC 1 cut(s) 277
BspACI CCGC 2 cut(s) 209, 211
BspANI GGCC 1 cut(s) 245
BspCNI CTCAG 3 cut(s) 88, 140, 174
BspFNI CGCG 1 cut(s) 211
BspLI GGNNCC 1 cut(s) 307
BspOI GCTAGC 1 cut(s) 18
BspQI GCTCTTC 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 305
BsrI ACTGG 3 cut(s) 275, 289, 374
BssECI CCNNGG 2 cut(s) 209, 294
BssMI GATC 1 cut(s) 277
BssNI GRCGYC 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 294
Bst4CI ACNGT 3 cut(s) 172, 189, 259
Bst6I CTCTTC 3 cut(s) 171, 355, 442
BstACI GRCGYC 1 cut(s) 306
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 3 cut(s) 96, 127, 182
BstDSI CCRYGG 1 cut(s) 209
BstFNI CGCG 1 cut(s) 211
BstH2I RGCGCY 1 cut(s) 309
BstHHI GCGC 1 cut(s) 308
BstKTI GATC 1 cut(s) 280
BstMAI GTCTC 2 cut(s) 178, 383
BstMBI GATC 1 cut(s) 277
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 109
BstUI CGCG 1 cut(s) 211
BsuRI GGCC 1 cut(s) 245
BtgI CCRYGG 1 cut(s) 209
BtrI CACGTC 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 1 cut(s) 16
CfoI GCGC 1 cut(s) 308
Cfr13I GGNCC 1 cut(s) 287
Cfr42I CCGCGG 1 cut(s) 212
Csp6I GTAC 2 cut(s) 346, 447
CviAII CATG 2 cut(s) 106, 455
CviJI RGCY 9 cut(s) 18, 47, 138, 181, 231, 245, 267, 397, 407
CviKI_1 RGCY 9 cut(s) 18, 47, 138, 181, 231, 245, 267, 397, 407
CviQI GTAC 2 cut(s) 346, 447
DdeI CTNAG 3 cut(s) 96, 127, 182
DinI GGCGCC 1 cut(s) 307
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 3 cut(s) 171, 355, 442
EarI CTCTTC 3 cut(s) 171, 355, 442
Eco130I CCWWGG 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 294
EgeI GGCGCC 1 cut(s) 307
EheI GGCGCC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 2 cut(s) 109, 458
FaiI YATR 4 cut(s) 92, 107, 248, 456
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 105, 454
FbaI TGATCA 1 cut(s) 277
FspBI CTAG 3 cut(s) 15, 66, 222
GlaI GCGC 1 cut(s) 307
HaeII RGCGCY 1 cut(s) 309
HaeIII GGCC 1 cut(s) 245
HhaI GCGC 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 2 cut(s) 109, 458
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
HincII GTYRAC 2 cut(s) 58, 367
HindII GTYRAC 2 cut(s) 58, 367
HinfI GANTC 1 cut(s) 363
HpaI GTTAAC 1 cut(s) 58
HphI GGTGA 2 cut(s) 198, 210
Hpy166II GTNNAC 2 cut(s) 58, 367
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 2 cut(s) 58, 367
HpyCH4III ACNGT 3 cut(s) 172, 189, 259
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 2 cut(s) 164, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 3 cut(s) 96, 127, 182
HpySE526I ACGT 1 cut(s) 86
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 2 cut(s) 109, 458
HspAI GCGC 1 cut(s) 306
KasI GGCGCC 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 277
KspAI GTTAAC 1 cut(s) 58
KspI CCGCGG 1 cut(s) 212
Kzo9I GATC 1 cut(s) 277
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 1 cut(s) 264
LweI GCATC 1 cut(s) 418
MaeI CTAG 3 cut(s) 15, 66, 222
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 1 cut(s) 216
MalI GATC 1 cut(s) 279
MboI GATC 1 cut(s) 277
MboII GAAGA 6 cut(s) 155, 188, 195, 372, 429, 432
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 3 cut(s) 25, 153, 249
MluNI TGGCCA 1 cut(s) 245
Mly113I GGCGCC 1 cut(s) 306
MlyI GAGTC 1 cut(s) 372
MnlI CCTC 5 cut(s) 319, 322, 346, 410, 462
Mox20I TGGCCA 1 cut(s) 245
MscI TGGCCA 1 cut(s) 245
MseI TTAA 2 cut(s) 57, 167
MslI CAYNNNNRTG 1 cut(s) 89
Msp20I TGGCCA 1 cut(s) 245
MspA1I CMGCKG 1 cut(s) 211
MvnI CGCG 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 11
NarI GGCGCC 1 cut(s) 306
NdeII GATC 1 cut(s) 277
NheI GCTAGC 1 cut(s) 14
NlaIII CATG 2 cut(s) 109, 458
NlaIV GGNNCC 1 cut(s) 307
NmuCI GTSAC 1 cut(s) 216
NspI RCATGY 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 171
Pfl23II CGTACG 1 cut(s) 446
PleI GAGTC 1 cut(s) 371
PluTI GGCGCC 1 cut(s) 309
PpsI GAGTC 1 cut(s) 371
PspLI CGTACG 1 cut(s) 446
PspN4I GGNNCC 1 cut(s) 307
PspPI GGNCC 1 cut(s) 287
RsaI GTAC 2 cut(s) 347, 448
RsaNI GTAC 2 cut(s) 346, 447
RseI CAYNNNNRTG 1 cut(s) 89
SacII CCGCGG 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 2 cut(s) 57, 167
Sau3AI GATC 1 cut(s) 277
Sau96I GGNCC 1 cut(s) 287
SchI GAGTC 1 cut(s) 372
SetI ASST 8 cut(s) 49, 89, 163, 183, 223, 269, 409, 421
SfaNI GCATC 1 cut(s) 418
SfoI GGCGCC 1 cut(s) 307
Sfr303I CCGCGG 1 cut(s) 212
SgrBI CCGCGG 1 cut(s) 212
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 89
Sse9I AATT 3 cut(s) 25, 153, 249
SsiI CCGC 2 cut(s) 209, 211
SspDI GGCGCC 1 cut(s) 305
SspMI CTAG 3 cut(s) 15, 66, 222
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 3 cut(s) 172, 189, 259
TaiI ACGT 1 cut(s) 89
TasI AATT 3 cut(s) 25, 153, 249
TatI WGTACW 1 cut(s) 345
Tru1I TTAA 2 cut(s) 57, 167
Tru9I TTAA 2 cut(s) 57, 167
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 216
TspDTI ATGAA 2 cut(s) 27, 189
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 287
XceI RCATGY 1 cut(s) 109
XspI CTAG 3 cut(s) 15, 66, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.