pycom10g15500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003544
Physical Location & Seq
Reverse (-)
18744661 .. 18745880
1220 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g15500.2

Sequence Viewer

Length: 807 bp
ATGGGTTGCTTTCTTGAATGTTTTGGTTCTTCCAAAGACAACAAGCGCAGGAGGAATCGGAGGTACAGGGTTCACCACCGAGACCATAGACATACAAGTTTCGAGCCTGTGAAATCTGCTCTCTCCTCTGCACCGGAGGTTGAGGAAAAACCTATTAGCCCGGCATTGGAAGAAGTCCGGGACAAGCCAGTGGAACAACTGAGCTTCAGTACTCGAAAGAAAGTTACTTTTGATTCGAATGTTAAGACCTATGAGCATGTTTCGACCAACGAAACTACGGATCCTTTGTTGGATAGTAAAGAGAATGGGAAGGAGGAGGAGGGGAAGAATTTGGAAAAACCGTGCCAATCTAAGTCTTCCTCTGAAGATAGTTCGGTCACTTCCAGCTCAGGGTCATACCCTCCTAACCATCGATACCAAAATTGCAGGGATAGCGATGATGAAGACGAAGTGTTAGACTATGATGAGGACAGTGATCTGGATGATGAAGATGAGGATGATTATGATGACGATGATGGGGAACTAGAGTACGAAGATGAAATTGTCGAATCAAATAGAGGGGTTTCTACTCCTCAAGTAATGACTGAGGATTTTGATAGTCCAATGCCGATGAAACTAGGTGGGTTGAACCACAGTGCACGCGATAGGAGTGGTTATGTTCATTCGGTGCTAAAGCCTGTTGAGAATCTAACACAATGGAAAGCTGTCAAAGCCAAAGGGACACCACTGATGAAGCCTCAGAAAGAGAATTTCACACAGGATCAATCTTTGAACCCCGGATTTCGTTCAGTTCAGAGCCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

269

Amino Acids

30.53

Weight (kDa)

4.75

Isoelectric Point (pI)

56.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 642
AclWI GGATC 3 cut(s) 275, 288, 768
AcsI RAATTY 2 cut(s) 328, 748
AcuI CTGAAG 2 cut(s) 190, 384
AfaI GTAC 3 cut(s) 65, 211, 530
AfiI CCNNNNNNNGG 2 cut(s) 166, 390
AgsI TTSAA 3 cut(s) 17, 628, 772
AluBI AGCT 3 cut(s) 204, 387, 704
AluI AGCT 3 cut(s) 204, 387, 704
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 1 cut(s) 75
Alw44I GTGCAC 1 cut(s) 636
AlwI GGATC 3 cut(s) 275, 288, 768
ApaLI GTGCAC 1 cut(s) 636
ApoI RAATTY 2 cut(s) 328, 748
AspLEI GCGC 1 cut(s) 48
AsuC2I CCSGG 3 cut(s) 161, 179, 777
AsuHPI GGTGA 1 cut(s) 65
AsuII TTCGAA 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 640
BamHI GGATCC 1 cut(s) 280
BbsI GAAGAC 2 cut(s) 348, 450
Bbv12I GWGCWC 1 cut(s) 640
BccI CCATC 2 cut(s) 417, 509
BcnI CCSGG 3 cut(s) 161, 179, 777
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 2 cut(s) 524, 617
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 3 cut(s) 161, 179, 777
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 3 cut(s) 161, 179, 777
BpiI GAAGAC 2 cut(s) 348, 450
Bpu10I CCTNAGC 1 cut(s) 388
Bpu14I TTCGAA 1 cut(s) 236
BpuEI CTTGAG 1 cut(s) 558
BpuMI CCSGG 3 cut(s) 161, 179, 777
Bsa29I ATCGAT 1 cut(s) 412
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 775
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 390
Bse1I ACTGG 1 cut(s) 188
BseCI ATCGAT 1 cut(s) 412
BseDI CCNNGG 1 cut(s) 775
BseGI GGATG 2 cut(s) 487, 502
BseLI CCNNNNNNNGG 2 cut(s) 166, 390
BseMII CTCAG 4 cut(s) 191, 402, 576, 752
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 4 cut(s) 115, 329, 332, 561
BseSI GKGCMC 1 cut(s) 640
BsgI GTGCAG 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 642
BshVI ATCGAT 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 4 cut(s) 134, 161, 178, 777
BslFI GGGAC 2 cut(s) 194, 733
BslI CCNNNNNNNGG 2 cut(s) 166, 390
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 2 cut(s) 194, 733
Bso31I GGTCTC 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 640
Bsp143I GATC 3 cut(s) 280, 475, 760
BspCNI CTCAG 4 cut(s) 192, 401, 577, 751
BspDI ATCGAT 1 cut(s) 412
BspFNI CGCG 1 cut(s) 642
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 3 cut(s) 275, 288, 768
BspT104I TTCGAA 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 75
BsrI ACTGG 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 775
BssMI GATC 3 cut(s) 280, 475, 760
Bst4CI ACNGT 3 cut(s) 342, 473, 635
BstBI TTCGAA 1 cut(s) 236
BstC8I GCNNGC 1 cut(s) 640
BstDEI CTNAG 5 cut(s) 200, 351, 388, 585, 738
BstF5I GGATG 2 cut(s) 487, 502
BstFNI CGCG 1 cut(s) 642
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 3 cut(s) 283, 478, 763
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 3 cut(s) 280, 475, 760
BstMWI GCNNNNNNNGC 2 cut(s) 432, 710
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 159, 177, 775
BstSLI GKGCMC 1 cut(s) 640
BstUI CGCG 1 cut(s) 642
BstV2I GAAGAC 2 cut(s) 348, 450
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
Bsu15I ATCGAT 1 cut(s) 412
BsuTUI ATCGAT 1 cut(s) 412
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 2 cut(s) 487, 502
BtsIMutI CAGTG 4 cut(s) 195, 478, 640, 725
Cac8I GCNNGC 1 cut(s) 640
CfoI GCGC 1 cut(s) 48
ClaI ATCGAT 1 cut(s) 412
Csp6I GTAC 3 cut(s) 64, 210, 529
CviAII CATG 1 cut(s) 257
CviQI GTAC 3 cut(s) 64, 210, 529
DdeI CTNAG 5 cut(s) 200, 351, 388, 585, 738
DpnI GATC 3 cut(s) 282, 477, 762
DpnII GATC 3 cut(s) 280, 475, 760
Eco31I GGTCTC 1 cut(s) 75
Eco57I CTGAAG 2 cut(s) 190, 384
FaeI CATG 1 cut(s) 260
FaiI YATR 8 cut(s) 87, 93, 252, 258, 397, 462, 504, 657
FaqI GGGAC 2 cut(s) 194, 733
FatI CATG 1 cut(s) 256
FokI GGATG 2 cut(s) 494, 509
FspBI CTAG 2 cut(s) 524, 617
GlaI GCGC 1 cut(s) 47
HapII CCGG 4 cut(s) 134, 161, 178, 777
HhaI GCGC 1 cut(s) 48
Hin1II CATG 1 cut(s) 260
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HinfI GANTC 4 cut(s) 55, 233, 548, 685
HpaII CCGG 4 cut(s) 134, 161, 178, 777
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 2 cut(s) 73, 638
Hpy188I TCNGA 4 cut(s) 60, 364, 741, 795
Hpy188III TCNNGA 2 cut(s) 14, 479
Hpy8I GTNNAC 2 cut(s) 73, 638
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 3 cut(s) 342, 473, 635
HpyCH4V TGCA 3 cut(s) 131, 426, 638
HpyF10VI GCNNNNNNNGC 2 cut(s) 432, 710
HpyF3I CTNAG 5 cut(s) 200, 351, 388, 585, 738
Hsp92II CATG 1 cut(s) 260
HspAI GCGC 1 cut(s) 46
Kzo9I GATC 3 cut(s) 280, 475, 760
MaeI CTAG 2 cut(s) 524, 617
MaeIII GTNAC 2 cut(s) 223, 376
MalI GATC 3 cut(s) 282, 477, 762
MboI GATC 3 cut(s) 280, 475, 760
MboII GAAGA 8 cut(s) 21, 182, 337, 348, 377, 455, 500, 545
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 640
MluCI AATT 4 cut(s) 328, 421, 540, 748
MmeI TCCRAC 1 cut(s) 270
MseI TTAA 1 cut(s) 243
MspI CCGG 4 cut(s) 134, 161, 178, 777
MspR9I CCNGG 3 cut(s) 161, 179, 777
MvnI CGCG 1 cut(s) 642
MwoI GCNNNNNNNGC 2 cut(s) 432, 710
NciI CCSGG 3 cut(s) 161, 179, 777
NdeII GATC 3 cut(s) 280, 475, 760
NlaIII CATG 1 cut(s) 260
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 376
NspI RCATGY 1 cut(s) 260
NspV TTCGAA 1 cut(s) 236
PfeI GAWTC 4 cut(s) 55, 233, 548, 685
PfoI TCCNGGA 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 282
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 3 cut(s) 65, 211, 530
RsaNI GTAC 3 cut(s) 64, 210, 529
SaqAI TTAA 1 cut(s) 243
Sau3AI GATC 3 cut(s) 280, 475, 760
ScaI AGTACT 1 cut(s) 211
ScrFI CCNGG 3 cut(s) 161, 179, 777
SduI GDGCHC 1 cut(s) 640
SetI ASST 8 cut(s) 65, 141, 154, 206, 251, 389, 622, 706
SfuI TTCGAA 1 cut(s) 236
SmlI CTYRAG 1 cut(s) 573
SmoI CTYRAG 1 cut(s) 573
Sse9I AATT 4 cut(s) 328, 421, 540, 748
SspMI CTAG 2 cut(s) 524, 617
StyD4I CCNGG 3 cut(s) 159, 177, 775
TaaI ACNGT 3 cut(s) 342, 473, 635
TaqI TCGA 6 cut(s) 102, 214, 236, 263, 412, 546
TaqII GACCGA 1 cut(s) 364
TasI AATT 4 cut(s) 328, 421, 540, 748
TatI WGTACW 1 cut(s) 209
TfiI GAWTC 4 cut(s) 55, 233, 548, 685
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 4 cut(s) 195, 478, 640, 732
TseFI GTSAC 1 cut(s) 376
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 6 cut(s) 456, 501, 552, 626, 650, 746
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 4 cut(s) 195, 478, 640, 732
VneI GTGCAC 1 cut(s) 636
XapI RAATTY 2 cut(s) 328, 748
XceI RCATGY 1 cut(s) 260
XspI CTAG 2 cut(s) 524, 617
ZrmI AGTACT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.