pycom05g29730

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001643
Physical Location & Seq
Forward (+)
30118293 .. 30122300
4008 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1236 bp
ATGAAGTCACCAAACTCATGGATATGGATGTTCAAGAAAAAGAATTCTTTTTCTGATGGGGGTAAAAGCGGAATGAAGATGAAGCAGCTGTCATTTATGGGAGTTGTATGTACAGTAATGTTATTCGTTGTGTATAGAACCACCAACTATCAGTATCAAGCTTATCAGCAGACAGAGATGGACGCAAAGTGGAATCCCTTTGACACAGTGAAGGAAATTTTTGTGACTTCGAGAAAGTTGGACGGTTTACCACGTGGCATTATACAACCTAGGTCAGATTTAGAGCAAAGGCCTCTATGGTTGACAAGTAGTTCAAGGTTAAAGGTCGGTGATTACAGCAACCGTAACTTGCTGGCTATTCCGGTTGGTATTAAGCAAAAGGATAGTGTTGATGCTATTGTGGGAAAGTTTCTTCCAGAAAATTTTACCGTTATCCTATTCCATTATGATGGGAATGTTGATGGGTGGTGGATCGATCTTGAGTGGAGTAACGACGCCATACACATAGTTGCCCAAAACCAAACAAAGTGGTGGTTTGCCAAACGCTTTCTACATCCAGATATTGTGTCCATTTATGATTACATATTCCTTTGGGATGAGGATTTGGGGGTTGAGAATTTTCATCCAGGAAGATACCTTGAAATTGTAAAAGAAGCGGGACTGGAGATATCTCAGCCAGCTTTGCATCCAAATTCAACAGATATACATCATAGAATTACAATTCGTGCTAAAAAAAAGAAGTTTCATAGAAGAGTCTATGACACCAGAGGCAGCACAAAGTGTACAGAAGCAAGTCAGGGACCACCATGCACCGGGTTTGTGGAAGGCATGGCTCCGGTTTTTTCAAGAGCTGCCTGGCATTGTACTTGGCATCTTATACAGAATGATCTTGTACATGGATGGGGTCTGGACATGAAACTTGGGTATTGTGCACAGGGAGACCGTACCAAGAAAGTCGGAGTTGTTGATAGTGAATATATTGTTCATAAGGGAATACAAACTTTGGGAGAAGGTGGTTCGTCGACAAACAAGGTTCCTGAGAAGAAGAGTAAGAAACGAGCTGCTCCTGGGCATGACATGCGAATTGAGATTAGGAGGCAGTCTACACGGGAGTATACAACCTTCAAAGAGCGGTGGAATAACGCAGTAGAAGAGGACTTGAACTGGATTGATCCATTTAAAAGCAGCCTAAAACGCAGGAAACAGCGTAGACGCAACAACCAGTTGAGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

412

Amino Acids

47.67

Weight (kDa)

9.53

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 79 - 384 1.9e-134 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1132
AccI GTMKAC 4 cut(s) 1022, 1103, 1115, 1210
AciI CCGC 3 cut(s) 69, 656, 1132
AclWI GGATC 2 cut(s) 479, 1166
AcsI RAATTY 5 cut(s) 43, 216, 421, 616, 691
AcvI CACGTG 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 495
AdeI CACNNNGTG 1 cut(s) 780
AfaI GTAC 5 cut(s) 112, 784, 865, 894, 946
AfiI CCNNNNNNNGG 1 cut(s) 812
AgsI TTSAA 7 cut(s) 34, 315, 641, 696, 846, 1126, 1162
AjnI CCWGG 3 cut(s) 625, 854, 1066
AluBI AGCT 6 cut(s) 88, 161, 680, 851, 1061, 1230
AluI AGCT 6 cut(s) 88, 161, 680, 851, 1061, 1230
Alw21I GWGCWC 2 cut(s) 934, 1232
Alw26I GTCTC 1 cut(s) 933
Alw44I GTGCAC 1 cut(s) 930
AlwI GGATC 2 cut(s) 479, 1166
AoxI GGCC 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 930
ApeKI GCWGC 5 cut(s) 85, 771, 851, 1061, 1185
ApoI RAATTY 5 cut(s) 43, 216, 421, 616, 691
AspA2I CCTAGG 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 800
AsuC2I CCSGG 1 cut(s) 814
AsuHPI GGTGA 1 cut(s) 341
AvaII GGWCC 1 cut(s) 800
AvrII CCTAGG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 1232
BarI GAAGNNNNNNTAC 2 cut(s) 1106, 1138
BbrPI CACGTG 1 cut(s) 254
Bbv12I GWGCWC 2 cut(s) 934, 1232
BbvI GCAGC 5 cut(s) 97, 783, 838, 1048, 1197
BccI CCATC 5 cut(s) 50, 172, 443, 455, 894
BciT130I CCWGG 3 cut(s) 627, 856, 1068
BcnI CCSGG 1 cut(s) 814
BcoDI GTCTC 1 cut(s) 933
BfaI CTAG 1 cut(s) 270
BisI GCNGC 5 cut(s) 86, 772, 852, 1062, 1186
BlnI CCTAGG 1 cut(s) 269
BlsI GCNGC 5 cut(s) 87, 773, 853, 1063, 1187
Bme1390I CCNGG 4 cut(s) 627, 814, 856, 1068
Bme18I GGWCC 1 cut(s) 800
BmgT120I GGNCC 1 cut(s) 800
BmiI GGNNCC 3 cut(s) 801, 834, 1035
BmrFI CCNGG 4 cut(s) 627, 814, 856, 1068
BmsI GCATC 3 cut(s) 382, 694, 880
BpmI CTGGAG 1 cut(s) 683
BpuEI CTTGAG 1 cut(s) 500
BpuMI CCSGG 1 cut(s) 814
Bsa29I ATCGAT 1 cut(s) 474
BsaAI YACGTR 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 705
BsaHI GRCGYC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 933
BsaJI CCNNGG 2 cut(s) 269, 1067
BsaWI WCCGGW 2 cut(s) 361, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 812
Bse1I ACTGG 3 cut(s) 666, 1169, 1222
Bse8I GATNNNNATC 1 cut(s) 705
BseBI CCWGG 3 cut(s) 627, 856, 1068
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 269, 1067
BseGI GGATG 6 cut(s) 33, 553, 601, 622, 685, 905
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 1 cut(s) 812
BseMII CTCAG 2 cut(s) 686, 1029
BseNI ACTGG 3 cut(s) 666, 1169, 1222
BseSI GKGCMC 1 cut(s) 934
BseXI GCAGC 5 cut(s) 97, 783, 838, 1048, 1197
BshFI GGCC 1 cut(s) 292
BshVI ATCGAT 1 cut(s) 474
BsiHKAI GWGCWC 2 cut(s) 934, 1232
BsiSI CCGG 3 cut(s) 362, 813, 836
BslFI GGGAC 2 cut(s) 672, 813
BslI CCNNNNNNNGG 1 cut(s) 812
BsmAI GTCTC 1 cut(s) 933
BsmFI GGGAC 2 cut(s) 672, 813
BsnI GGCC 1 cut(s) 292
Bso31I GGTCTC 1 cut(s) 933
Bsp1286I GDGCHC 2 cut(s) 934, 1232
Bsp1407I TGTACA 3 cut(s) 110, 782, 892
Bsp143I GATC 4 cut(s) 471, 475, 886, 1171
BspACI CCGC 3 cut(s) 69, 656, 1132
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 2 cut(s) 685, 1030
BspDI ATCGAT 1 cut(s) 474
BspLI GGNNCC 3 cut(s) 801, 834, 1035
BspPI GGATC 2 cut(s) 479, 1166
BspTNI GGTCTC 1 cut(s) 933
BsrBI CCGCTC 1 cut(s) 1132
BsrGI TGTACA 3 cut(s) 110, 782, 892
BsrI ACTGG 3 cut(s) 666, 1169, 1222
BssECI CCNNGG 2 cut(s) 269, 1067
BssMI GATC 4 cut(s) 471, 475, 886, 1171
BssNAI GTATAC 1 cut(s) 1116
BssNI GRCGYC 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 269
Bst1107I GTATAC 1 cut(s) 1116
Bst2UI CCWGG 3 cut(s) 627, 856, 1068
Bst4CI ACNGT 6 cut(s) 115, 208, 245, 344, 430, 944
Bst6I CTCTTC 3 cut(s) 745, 1040, 1146
BstACI GRCGYC 1 cut(s) 495
BstAPI GCANNNNNTGC 1 cut(s) 1078
BstAUI TGTACA 3 cut(s) 110, 782, 892
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 2 cut(s) 354, 678
BstDEI CTNAG 2 cut(s) 672, 1038
BstF5I GGATG 6 cut(s) 33, 553, 601, 622, 685, 905
BstKTI GATC 4 cut(s) 474, 478, 889, 1174
BstMAI GTCTC 1 cut(s) 933
BstMBI GATC 4 cut(s) 471, 475, 886, 1171
BstMWI GCNNNNNNNGC 3 cut(s) 682, 1078, 1194
BstNI CCWGG 3 cut(s) 627, 856, 1068
BstNSI RCATGY 1 cut(s) 1081
BstSCI CCNGG 4 cut(s) 625, 812, 854, 1066
BstSLI GKGCMC 1 cut(s) 934
BstV1I GCAGC 5 cut(s) 97, 783, 838, 1048, 1197
BstXI CCANNNNNNTGG 2 cut(s) 18, 449
BstZ17I GTATAC 1 cut(s) 1116
Bsu15I ATCGAT 1 cut(s) 474
BsuRI GGCC 1 cut(s) 292
BsuTUI ATCGAT 1 cut(s) 474
BtsCI GGATG 6 cut(s) 33, 553, 601, 622, 685, 905
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 354, 678
Cfr13I GGNCC 1 cut(s) 800
ClaI ATCGAT 1 cut(s) 474
CseI GACGC 3 cut(s) 191, 503, 1221
Csp6I GTAC 5 cut(s) 111, 783, 864, 893, 945
CspCI CAANNNNNGTGG 2 cut(s) 509, 544
CviAII CATG 7 cut(s) 18, 807, 829, 896, 913, 1073, 1078
CviQI GTAC 5 cut(s) 111, 783, 864, 893, 945
DdeI CTNAG 2 cut(s) 672, 1038
DpnI GATC 4 cut(s) 473, 477, 888, 1173
DpnII GATC 4 cut(s) 471, 475, 886, 1171
DraI TTTAAA 1 cut(s) 1180
DraIII CACNNNGTG 1 cut(s) 780
Eam1104I CTCTTC 3 cut(s) 745, 1040, 1146
EarI CTCTTC 3 cut(s) 745, 1040, 1146
Ecl136II GAGCTC 1 cut(s) 1230
Eco130I CCWWGG 1 cut(s) 269
Eco147I AGGCCT 1 cut(s) 292
Eco24I GRGCYC 1 cut(s) 1232
Eco31I GGTCTC 1 cut(s) 933
Eco32I GATATC 1 cut(s) 669
Eco47I GGWCC 1 cut(s) 800
Eco53kI GAGCTC 1 cut(s) 1230
Eco72I CACGTG 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 1230
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 3 cut(s) 625, 854, 1066
EcoRV GATATC 1 cut(s) 669
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 1232
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 7 cut(s) 21, 810, 832, 899, 916, 1076, 1081
FaqI GGGAC 2 cut(s) 672, 813
FatI CATG 7 cut(s) 17, 806, 828, 895, 912, 1072, 1077
FauI CCCGC 1 cut(s) 649
FblI GTMKAC 4 cut(s) 1022, 1103, 1115, 1210
Fnu4HI GCNGC 5 cut(s) 86, 772, 852, 1062, 1186
FokI GGATG 6 cut(s) 40, 540, 608, 609, 672, 912
FriOI GRGCYC 1 cut(s) 1232
Fsp4HI GCNGC 5 cut(s) 86, 772, 852, 1062, 1186
FspBI CTAG 1 cut(s) 270
GluI GCNGC 5 cut(s) 86, 772, 852, 1062, 1186
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 1 cut(s) 292
HapII CCGG 3 cut(s) 362, 813, 836
HgaI GACGC 3 cut(s) 191, 503, 1221
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 7 cut(s) 21, 810, 832, 899, 916, 1076, 1081
HincII GTYRAC 2 cut(s) 303, 1023
HindII GTYRAC 2 cut(s) 303, 1023
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 2 cut(s) 193, 753
HpaII CCGG 3 cut(s) 362, 813, 836
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 8 cut(s) 248, 303, 783, 932, 1023, 1104, 1116, 1211
Hpy188I TCNGA 3 cut(s) 55, 277, 959
Hpy188III TCNNGA 8 cut(s) 34, 231, 416, 479, 557, 846, 908, 1037
Hpy8I GTNNAC 8 cut(s) 248, 303, 783, 932, 1023, 1104, 1116, 1211
Hpy99I CGWCG 2 cut(s) 497, 1024
HpyAV CCTTC 4 cut(s) 205, 818, 1004, 1132
HpyCH4III ACNGT 6 cut(s) 115, 208, 245, 344, 430, 944
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 3 cut(s) 685, 810, 932
HpyF10VI GCNNNNNNNGC 3 cut(s) 682, 1078, 1194
HpyF3I CTNAG 2 cut(s) 672, 1038
HpySE526I ACGT 1 cut(s) 253
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 7 cut(s) 21, 810, 832, 899, 916, 1076, 1081
Kzo9I GATC 4 cut(s) 471, 475, 886, 1171
LmnI GCTCC 2 cut(s) 838, 1069
Lsp1109I GCAGC 5 cut(s) 97, 783, 838, 1048, 1197
LweI GCATC 3 cut(s) 382, 694, 880
MaeI CTAG 1 cut(s) 270
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 4 cut(s) 6, 223, 344, 488
MalI GATC 4 cut(s) 473, 477, 888, 1173
MbiI CCGCTC 1 cut(s) 1132
MboI GATC 4 cut(s) 471, 475, 886, 1171
MboII GAAGA 7 cut(s) 88, 404, 642, 762, 1054, 1057, 1163
MhlI GDGCHC 2 cut(s) 934, 1232
MluCI AATT 9 cut(s) 43, 216, 421, 616, 642, 691, 714, 720, 1083
MlyI GAGTC 1 cut(s) 762
MmeI TCCRAC 2 cut(s) 219, 937
MnlI CCTC 5 cut(s) 303, 592, 761, 1089, 1147
MseI TTAA 4 cut(s) 320, 372, 1179, 1234
MslI CAYNNNNRTG 2 cut(s) 22, 447
MspA1I CMGCKG 1 cut(s) 88
MspI CCGG 3 cut(s) 362, 813, 836
MspR9I CCNGG 4 cut(s) 627, 814, 856, 1068
MvaI CCWGG 3 cut(s) 627, 856, 1068
MwoI GCNNNNNNNGC 3 cut(s) 682, 1078, 1194
NciI CCSGG 1 cut(s) 814
NdeII GATC 4 cut(s) 471, 475, 886, 1171
NlaIII CATG 7 cut(s) 21, 810, 832, 899, 916, 1076, 1081
NlaIV GGNNCC 3 cut(s) 801, 834, 1035
NmuCI GTSAC 2 cut(s) 6, 223
NspI RCATGY 1 cut(s) 1081
PceI AGGCCT 1 cut(s) 292
PfeI GAWTC 1 cut(s) 193
PfoI TCCNGGA 1 cut(s) 625
PkrI GCNGC 5 cut(s) 87, 773, 853, 1063, 1187
PleI GAGTC 1 cut(s) 761
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
PpsI GAGTC 1 cut(s) 761
Ppu21I YACGTR 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 1232
Psp6I CCWGG 3 cut(s) 625, 854, 1066
PspCI CACGTG 1 cut(s) 254
PspGI CCWGG 3 cut(s) 625, 854, 1066
PspN4I GGNNCC 3 cut(s) 801, 834, 1035
PspPI GGNCC 1 cut(s) 800
PvuII CAGCTG 1 cut(s) 88
RsaI GTAC 5 cut(s) 112, 784, 865, 894, 946
RsaNI GTAC 5 cut(s) 111, 783, 864, 893, 945
RseI CAYNNNNRTG 2 cut(s) 22, 447
SacI GAGCTC 1 cut(s) 1232
SalI GTCGAC 1 cut(s) 1021
SaqAI TTAA 4 cut(s) 320, 372, 1179, 1234
SatI GCNGC 5 cut(s) 86, 772, 852, 1062, 1186
Sau3AI GATC 4 cut(s) 471, 475, 886, 1171
Sau96I GGNCC 1 cut(s) 800
SchI GAGTC 1 cut(s) 762
ScrFI CCNGG 4 cut(s) 627, 814, 856, 1068
SduI GDGCHC 2 cut(s) 934, 1232
SfaNI GCATC 3 cut(s) 382, 694, 880
SinI GGWCC 1 cut(s) 800
SmiMI CAYNNNNRTG 2 cut(s) 22, 447
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 9 cut(s) 43, 216, 421, 616, 642, 691, 714, 720, 1083
SseBI AGGCCT 1 cut(s) 292
SsiI CCGC 3 cut(s) 69, 656, 1132
SspMI CTAG 1 cut(s) 270
SstI GAGCTC 1 cut(s) 1232
StuI AGGCCT 1 cut(s) 292
StyD4I CCNGG 4 cut(s) 625, 812, 854, 1066
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 6 cut(s) 115, 208, 245, 344, 430, 944
TaiI ACGT 1 cut(s) 256
TaqI TCGA 3 cut(s) 230, 474, 1022
TasI AATT 9 cut(s) 43, 216, 421, 616, 642, 691, 714, 720, 1083
TatI WGTACW 4 cut(s) 110, 782, 863, 892
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 4 cut(s) 320, 372, 1179, 1234
Tru9I TTAA 4 cut(s) 320, 372, 1179, 1234
TscAI CASTG 1 cut(s) 213
TseFI GTSAC 2 cut(s) 6, 223
TseI GCWGC 5 cut(s) 85, 771, 851, 1061, 1185
Tsp45I GTSAC 2 cut(s) 6, 223
TspDTI ATGAA 7 cut(s) 17, 89, 95, 611, 734, 929, 974
TspRI CASTG 1 cut(s) 213
VneI GTGCAC 1 cut(s) 930
VpaK11BI GGWCC 1 cut(s) 800
XapI RAATTY 5 cut(s) 43, 216, 421, 616, 691
XceI RCATGY 1 cut(s) 1081
XmaJI CCTAGG 1 cut(s) 269
XmiI GTMKAC 4 cut(s) 1022, 1103, 1115, 1210
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.