pycom06g03380

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003066
Physical Location & Seq
Reverse (-)
4261698 .. 4264271
2574 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
ATGACAATAGACACCGCCACCATGGGAGAGCAAGAGCAGCTGCGCCTCCAACTCTCTGCCCTCCACGTAAACACTCCGCCGCCTCTTTACCAAAACTTGTATGAGTCTGATGACCAACAAGTCTTCTACAACGACGACGGCTTGCTGTTCGACATCGGGTTCGGAAACGTGATTGTCGTCGATAACCTTCCAATTGTTGATGCCAGGATGGCCGAGAAGCTCGAAAGCGAAATCCGACAAGCGTTTAGTGAAGTTGGTGTGATTAAACACGATGGGGTTTCCGTGCCTCTGAATCCCCAAACCCACGAAATCTTGGGTCGTTGTTTTATAGAGTTTAATTCTCGTCAGGAAGCGGAACTTGCTAGGGAGAAGAGAGATGGATACAACTTCCATACACATACACTATCAGTAAGCCTGTATGATGACTTGGACAGGTTCGTTGATATCCTTGATGAGGGGACTACTCCAGAAACTTATCCAGCCGCTTGTATGTCCTCAAGGTATATGGGGCCATCCCCGTTGCTTAGTCCCTTGAAAGAGGAAGAAGTACCGGAAGAAGCCGATCGAGTAAACCACGGCAGAGAAGCAAGAGAGCAAAAGGAGCAAGATTTCGGGGAAGTCATGGCATCAATTGGAATGATGAGTATTAGAGGGGAAAGCATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

223

Amino Acids

25.12

Weight (kDa)

4.44

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 75 - 135 2.5e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AciI CCGC 5 cut(s) 15, 77, 80, 353, 483
AcoI YGGCCR 1 cut(s) 210
AfaI GTAC 1 cut(s) 549
AfiI CCNNNNNNNGG 1 cut(s) 454
AgsI TTSAA 1 cut(s) 535
AjnI CCWGG 1 cut(s) 203
AluBI AGCT 2 cut(s) 40, 220
AluI AGCT 2 cut(s) 40, 220
AoxI GGCC 2 cut(s) 210, 509
ApeKI GCWGC 2 cut(s) 37, 40
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 509
BbsI GAAGAC 1 cut(s) 115
BbvI GCAGC 2 cut(s) 27, 49
BccI CCATC 4 cut(s) 202, 266, 371, 520
BceAI ACGGC 2 cut(s) 154, 592
BciT130I CCWGG 1 cut(s) 205
BciVI GTATCC 1 cut(s) 374
BfaI CTAG 1 cut(s) 363
BfuI GTATCC 1 cut(s) 374
BglI GCCNNNNNGGC 1 cut(s) 209
BisI GCNGC 4 cut(s) 38, 41, 80, 483
BlsI GCNGC 4 cut(s) 39, 42, 81, 484
Bme1390I CCNGG 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 509
BmiI GGNNCC 1 cut(s) 510
BmrFI CCNGG 1 cut(s) 205
BmsI GCATC 2 cut(s) 190, 635
BpiI GAAGAC 1 cut(s) 115
BpmI CTGGAG 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 481
BsaAI YACGTR 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 21, 574
BsaWI WCCGGW 1 cut(s) 550
Bsc4I CCNNNNNNNGG 1 cut(s) 454
BseBI CCWGG 1 cut(s) 205
BseDI CCNNGG 2 cut(s) 21, 574
BseGI GGATG 2 cut(s) 213, 512
BseLI CCNNNNNNNGG 1 cut(s) 454
BseXI GCAGC 2 cut(s) 27, 49
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 2 cut(s) 212, 511
BsiEI CGRYCG 1 cut(s) 565
BsiSI CCGG 1 cut(s) 551
BslFI GGGAC 2 cut(s) 472, 513
BslI CCNNNNNNNGG 1 cut(s) 454
BsmFI GGGAC 2 cut(s) 472, 513
BsnI GGCC 2 cut(s) 212, 511
Bsp143I GATC 1 cut(s) 562
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 5 cut(s) 15, 77, 80, 353, 483
BspANI GGCC 2 cut(s) 212, 511
BspLI GGNNCC 1 cut(s) 510
BssECI CCNNGG 2 cut(s) 21, 574
BssMI GATC 1 cut(s) 562
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 205
Bst6I CTCTTC 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 143
BstDEI CTNAG 1 cut(s) 524
BstDSI CCRYGG 2 cut(s) 21, 574
BstENI CCTNNNNNAGG 1 cut(s) 452
BstF5I GGATG 2 cut(s) 213, 512
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 1 cut(s) 565
BstMBI GATC 1 cut(s) 562
BstMCI CGRYCG 1 cut(s) 565
BstMWI GCNNNNNNNGC 4 cut(s) 37, 209, 359, 601
BstNI CCWGG 1 cut(s) 205
BstSCI CCNGG 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 27, 49
BstV2I GAAGAC 1 cut(s) 115
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 2 cut(s) 212, 511
BtgI CCRYGG 2 cut(s) 21, 574
BtsCI GGATG 2 cut(s) 213, 512
Cac8I GCNNGC 1 cut(s) 143
CfoI GCGC 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 509
Csp6I GTAC 1 cut(s) 548
CviAII CATG 3 cut(s) 22, 622, 661
CviJI RGCY 8 cut(s) 40, 141, 212, 220, 414, 482, 511, 560
CviKI_1 RGCY 8 cut(s) 40, 141, 212, 220, 414, 482, 511, 560
CviQI GTAC 1 cut(s) 548
DdeI CTNAG 1 cut(s) 524
DpnI GATC 1 cut(s) 564
DpnII GATC 1 cut(s) 562
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
EciI GGCGGA 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 21
Eco32I GATATC 1 cut(s) 445
EcoNI CCTNNNNNAGG 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 203
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 3 cut(s) 25, 625, 664
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FaqI GGGAC 2 cut(s) 472, 513
FatI CATG 3 cut(s) 21, 621, 660
Fnu4HI GCNGC 4 cut(s) 38, 41, 80, 483
FokI GGATG 2 cut(s) 220, 499
Fsp4HI GCNGC 4 cut(s) 38, 41, 80, 483
FspBI CTAG 1 cut(s) 363
GlaI GCGC 1 cut(s) 44
GluI GCNGC 4 cut(s) 38, 41, 80, 483
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 2 cut(s) 212, 511
HapII CCGG 1 cut(s) 551
HhaI GCGC 1 cut(s) 45
Hin1II CATG 3 cut(s) 25, 625, 664
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HinfI GANTC 2 cut(s) 104, 292
HpaII CCGG 1 cut(s) 551
Hpy166II GTNNAC 2 cut(s) 70, 571
Hpy188I TCNGA 4 cut(s) 109, 164, 236, 291
Hpy188III TCNNGA 2 cut(s) 347, 467
Hpy8I GTNNAC 2 cut(s) 70, 571
Hpy99I CGWCG 3 cut(s) 137, 140, 182
HpyAV CCTTC 1 cut(s) 197
HpyCH4IV ACGT 2 cut(s) 66, 168
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 209, 359, 601
HpyF3I CTNAG 1 cut(s) 524
HpySE526I ACGT 2 cut(s) 66, 168
Hsp92II CATG 3 cut(s) 25, 625, 664
HspAI GCGC 1 cut(s) 43
Kzo9I GATC 1 cut(s) 562
LmnI GCTCC 1 cut(s) 601
LpnPI CCDG 8 cut(s) 190, 217, 332, 418, 428, 480, 492, 564
Lsp1109I GCAGC 2 cut(s) 27, 49
LweI GCATC 2 cut(s) 190, 635
MaeI CTAG 1 cut(s) 363
MaeII ACGT 2 cut(s) 66, 168
MalI GATC 1 cut(s) 564
MboI GATC 1 cut(s) 562
MboII GAAGA 4 cut(s) 115, 382, 554, 566
MfeI CAATTG 2 cut(s) 192, 630
MluCI AATT 3 cut(s) 192, 337, 630
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 2 cut(s) 73, 259
MnlI CCTC 8 cut(s) 56, 71, 93, 297, 448, 505, 532, 644
MseI TTAA 2 cut(s) 264, 336
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 1 cut(s) 551
MspR9I CCNGG 1 cut(s) 205
MunI CAATTG 2 cut(s) 192, 630
MvaI CCWGG 1 cut(s) 205
MwoI GCNNNNNNNGC 4 cut(s) 37, 209, 359, 601
NcoI CCATGG 1 cut(s) 21
NdeII GATC 1 cut(s) 562
NlaIII CATG 3 cut(s) 25, 625, 664
NlaIV GGNNCC 1 cut(s) 510
NmeAIII GCCGAG 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 1 cut(s) 292
PkrI GCNGC 4 cut(s) 39, 42, 81, 484
Ple19I CGATCG 1 cut(s) 565
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
Ppu21I YACGTR 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 203
PspGI CCWGG 1 cut(s) 203
PspN4I GGNNCC 1 cut(s) 510
PspPI GGNCC 1 cut(s) 509
PvuI CGATCG 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 1 cut(s) 549
RsaNI GTAC 1 cut(s) 548
SaqAI TTAA 2 cut(s) 264, 336
SatI GCNGC 4 cut(s) 38, 41, 80, 483
Sau3AI GATC 1 cut(s) 562
Sau96I GGNCC 1 cut(s) 509
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 1 cut(s) 205
SetI ASST 7 cut(s) 42, 69, 171, 189, 222, 437, 503
SfaNI GCATC 2 cut(s) 190, 635
SmlI CTYRAG 1 cut(s) 496
SmoI CTYRAG 1 cut(s) 496
Sse9I AATT 3 cut(s) 192, 337, 630
SsiI CCGC 5 cut(s) 15, 77, 80, 353, 483
SspMI CTAG 1 cut(s) 363
StyD4I CCNGG 1 cut(s) 203
StyI CCWWGG 1 cut(s) 21
TaiI ACGT 2 cut(s) 69, 171
TaqI TCGA 4 cut(s) 150, 180, 222, 565
TasI AATT 3 cut(s) 192, 337, 630
TauI GCSGC 2 cut(s) 82, 485
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 2 cut(s) 264, 336
Tru9I TTAA 2 cut(s) 264, 336
TseI GCWGC 2 cut(s) 37, 40
TspGWI ACGGA 1 cut(s) 271
XagI CCTNNNNNAGG 1 cut(s) 452
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.