pycom07g17740

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004474
Physical Location & Seq
Reverse (-)
20276806 .. 20278711
1906 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
ATGCTCGTCGACACTCCCCCGCCTTCCCTTGAGGGGCCGGTGAAACACCCTCCACGTTTTTTAACACTCCCACCTGCCTCTTCTTACTCATCAAACCTCCTCCCATTACTCAGTGGACCGACCAATTACCACCTCTATTTAAACCCACCGTTCCCGACAGCTAACCCCATCTCTCTCTCTGCACAATATATAAATTATCAAATGGCTCCACCACCATCGCTCGCTGGACTCCAGGTCGAGGCCACTGCGTTTCCACCGTCCGTCAACCCTCCGGGCTCCTCCAACACTTTGTTCCTCGGCGGCGCAGGGGTGAGGGGGCTGGAGATTCAGGGCACCTTCGTGAAGTTCACGGCGATCGGAGTGTACTTGGAGGATAACGCCGTGCCTCTGCTAGCCGTTAAGTGGAAGGGTAAGACCGCCGAGGAGTTGTCGGAGTCCGTTGAGTTCTTCAGGGACATCGTTACAGGTCCGTTTGAGAAGTTCATTCAGGTGACAATGATACTGCCACTGACGGGCCAGCAATACTCTGAGAAAGTTTCGGAGAATTGCGTTGCCTTTTGGAAGTCGGTGGGAATTTACACTGATGCAGAAGGCAAAGCCATTGAAAAGTTCATTGAGGTCTTCAAAGATCAAAACTTCCCACCGGGCGCCTCTATTCTCTTCACACAATCTCCGAAAGGATCACTCACGATTTGCTTCTCCAAAGATGCATCCGTGCCAGAAGCTGCAAATGCGGTGATAGAAAACAAACTACTTTCCGAGGCAGTTCTAGAGATCGATCGTGGGAAAGCACGGTGTTTCCCCTGCAGCAAAGCGAAGTTTGGCCGCGAGGTTATCCGAACTGTTGAACGGGTGTAA

Protein Analysis

286

Amino Acids

30.94

Weight (kDa)

5.84

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 79 - 272 2.2e-94 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 82
Acc36I ACCTGC 1 cut(s) 82
AccB1I GGYRCC 2 cut(s) 332, 647
AccI GTMKAC 1 cut(s) 9
AccII CGCG 1 cut(s) 828
AciI CCGC 5 cut(s) 20, 300, 417, 734, 826
AclWI GGATC 1 cut(s) 688
AcoI YGGCCR 1 cut(s) 823
AcsI RAATTY 1 cut(s) 573
AcuI CTGAAG 1 cut(s) 433
AcyI GRCGYC 1 cut(s) 648
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 4 cut(s) 33, 238, 402, 512
AgsI TTSAA 3 cut(s) 605, 625, 848
AhdI GACNNNNNGTC 1 cut(s) 233
AjnI CCWGG 1 cut(s) 231
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 2 cut(s) 161, 725
AluI AGCT 2 cut(s) 161, 725
AlwI GGATC 1 cut(s) 688
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 4 cut(s) 35, 240, 514, 823
ApeKI GCWGC 2 cut(s) 725, 807
ApoI RAATTY 1 cut(s) 573
AspLEI GCGC 2 cut(s) 305, 650
AspS9I GGNCC 4 cut(s) 35, 116, 467, 514
AsuC2I CCSGG 2 cut(s) 273, 645
AsuHPI GGTGA 4 cut(s) 52, 322, 502, 748
AsuNHI GCTAGC 1 cut(s) 391
AvaII GGWCC 2 cut(s) 116, 467
BaeGI GKGCMC 1 cut(s) 335
BanI GGYRCC 2 cut(s) 332, 647
BanII GRGCYC 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 613
BbvI GCAGC 2 cut(s) 712, 819
BccI CCATC 2 cut(s) 176, 223
BceAI ACGGC 3 cut(s) 365, 366, 380
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 1 cut(s) 233
BcnI CCSGG 2 cut(s) 273, 645
BfaI CTAG 2 cut(s) 392, 770
BfmI CTRYAG 1 cut(s) 805
BfoI RGCGCY 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 82
BisI GCNGC 4 cut(s) 301, 726, 808, 826
BlsI GCNGC 4 cut(s) 302, 727, 809, 827
Bme1390I CCNGG 3 cut(s) 233, 273, 645
Bme18I GGWCC 2 cut(s) 116, 467
BmeRI GACNNNNNGTC 1 cut(s) 233
BmgT120I GGNCC 4 cut(s) 35, 116, 467, 514
BmiI GGNNCC 5 cut(s) 36, 207, 277, 334, 649
BmrFI CCNGG 3 cut(s) 233, 273, 645
BmsI GCATC 3 cut(s) 574, 697, 719
BmtI GCTAGC 1 cut(s) 395
BpiI GAAGAC 1 cut(s) 613
BpmI CTGGAG 2 cut(s) 215, 341
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 2 cut(s) 273, 645
Bsa29I ATCGAT 1 cut(s) 777
BsaHI GRCGYC 1 cut(s) 648
BsaJI CCNNGG 3 cut(s) 295, 420, 759
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 238, 402, 512
Bse118I RCCGGY 1 cut(s) 37
BseBI CCWGG 1 cut(s) 233
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 3 cut(s) 295, 420, 759
BseGI GGATG 1 cut(s) 710
BseLI CCNNNNNNNGG 4 cut(s) 33, 238, 402, 512
BseMII CTCAG 2 cut(s) 124, 519
BseRI GAGGAG 3 cut(s) 89, 268, 437
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 2 cut(s) 712, 819
BsgI GTGCAG 1 cut(s) 165
Bsh1236I CGCG 1 cut(s) 828
Bsh1285I CGRYCG 2 cut(s) 357, 781
BshFI GGCC 4 cut(s) 37, 242, 516, 825
BshNI GGYRCC 2 cut(s) 332, 647
BshVI ATCGAT 1 cut(s) 777
BsiEI CGRYCG 2 cut(s) 357, 781
BsiSI CCGG 3 cut(s) 38, 272, 644
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 4 cut(s) 33, 238, 402, 512
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 4 cut(s) 37, 242, 516, 825
Bsp1286I GDGCHC 2 cut(s) 278, 335
Bsp143I GATC 5 cut(s) 354, 628, 680, 774, 778
BspACI CCGC 5 cut(s) 20, 300, 417, 734, 826
BspANI GGCC 4 cut(s) 37, 242, 516, 825
BspCNI CTCAG 2 cut(s) 123, 520
BspDI ATCGAT 1 cut(s) 777
BspFNI CGCG 1 cut(s) 828
BspLI GGNNCC 5 cut(s) 36, 207, 277, 334, 649
BspMAI CTGCAG 1 cut(s) 809
BspMI ACCTGC 1 cut(s) 82
BspOI GCTAGC 1 cut(s) 395
BspPI GGATC 1 cut(s) 688
BspT107I GGYRCC 2 cut(s) 332, 647
BsrFI RCCGGY 1 cut(s) 37
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 295, 420, 759
BssMI GATC 5 cut(s) 354, 628, 680, 774, 778
BssNI GRCGYC 1 cut(s) 648
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 4 cut(s) 150, 258, 795, 844
Bst6I CTCTTC 2 cut(s) 85, 665
BstACI GRCGYC 1 cut(s) 648
BstC8I GCNNGC 3 cut(s) 222, 393, 518
BstDEI CTNAG 2 cut(s) 110, 528
BstF5I GGATG 1 cut(s) 710
BstFNI CGCG 1 cut(s) 828
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 2 cut(s) 305, 650
BstKTI GATC 5 cut(s) 357, 631, 683, 777, 781
BstMBI GATC 5 cut(s) 354, 628, 680, 774, 778
BstMCI CGRYCG 2 cut(s) 357, 781
BstMWI GCNNNNNNNGC 1 cut(s) 731
BstNI CCWGG 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 231, 271, 643
BstSFI CTRYAG 1 cut(s) 805
BstSLI GKGCMC 1 cut(s) 335
BstUI CGCG 1 cut(s) 828
BstV1I GCAGC 2 cut(s) 712, 819
BstV2I GAAGAC 1 cut(s) 613
Bsu15I ATCGAT 1 cut(s) 777
BsuRI GGCC 4 cut(s) 37, 242, 516, 825
BsuTUI ATCGAT 1 cut(s) 777
BtgZI GCGATG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 710
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 4 cut(s) 118, 243, 506, 579
BveI ACCTGC 1 cut(s) 82
Cac8I GCNNGC 3 cut(s) 222, 393, 518
CaiI CAGNNNCTG 1 cut(s) 725
CfoI GCGC 2 cut(s) 305, 650
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 4 cut(s) 35, 116, 467, 514
ClaI ATCGAT 1 cut(s) 777
Csp6I GTAC 1 cut(s) 364
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 2 cut(s) 110, 528
DinI GGCGCC 1 cut(s) 649
DpnI GATC 5 cut(s) 356, 630, 682, 776, 780
DpnII GATC 5 cut(s) 354, 628, 680, 774, 778
DraI TTTAAA 1 cut(s) 141
DriI GACNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 823
Eam1104I CTCTTC 2 cut(s) 85, 665
Eam1105I GACNNNNNGTC 1 cut(s) 233
EarI CTCTTC 2 cut(s) 85, 665
Eco24I GRGCYC 1 cut(s) 278
Eco47I GGWCC 2 cut(s) 116, 467
Eco57I CTGAAG 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 231
EcoT22I ATGCAT 1 cut(s) 712
EcoT38I GRGCYC 1 cut(s) 278
EgeI GGCGCC 1 cut(s) 649
EheI GGCGCC 1 cut(s) 649
FaiI YATR 2 cut(s) 189, 191
FaqI GGGAC 1 cut(s) 467
FauI CCCGC 1 cut(s) 27
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 4 cut(s) 301, 726, 808, 826
FokI GGATG 1 cut(s) 697
FriOI GRGCYC 1 cut(s) 278
Fsp4HI GCNGC 4 cut(s) 301, 726, 808, 826
FspBI CTAG 2 cut(s) 392, 770
GlaI GCGC 2 cut(s) 304, 649
GluI GCNGC 4 cut(s) 301, 726, 808, 826
GsuI CTGGAG 2 cut(s) 215, 341
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 4 cut(s) 37, 242, 516, 825
HapII CCGG 3 cut(s) 38, 272, 644
HhaI GCGC 2 cut(s) 305, 650
Hin1I GRCGYC 1 cut(s) 648
Hin6I GCGC 2 cut(s) 303, 648
HinP1I GCGC 2 cut(s) 303, 648
HincII GTYRAC 2 cut(s) 10, 265
HindII GTYRAC 2 cut(s) 10, 265
HinfI GANTC 3 cut(s) 228, 325, 434
HpaII CCGG 3 cut(s) 38, 272, 644
HphI GGTGA 4 cut(s) 52, 322, 502, 748
Hpy166II GTNNAC 5 cut(s) 10, 116, 265, 348, 364
Hpy188I TCNGA 7 cut(s) 359, 433, 529, 541, 675, 760, 839
Hpy188III TCNNGA 4 cut(s) 154, 340, 688, 770
Hpy8I GTNNAC 5 cut(s) 10, 116, 265, 348, 364
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 4 cut(s) 33, 346, 400, 584
HpyCH4III ACNGT 4 cut(s) 150, 258, 795, 844
HpyCH4IV ACGT 1 cut(s) 55
HpyCH4V TGCA 5 cut(s) 182, 587, 710, 728, 807
HpyF10VI GCNNNNNNNGC 1 cut(s) 731
HpyF3I CTNAG 2 cut(s) 110, 528
HpySE526I ACGT 1 cut(s) 55
Hsp92I GRCGYC 1 cut(s) 648
HspAI GCGC 2 cut(s) 303, 648
KasI GGCGCC 1 cut(s) 647
Kzo9I GATC 5 cut(s) 354, 628, 680, 774, 778
LmnI GCTCC 2 cut(s) 211, 281
Lsp1109I GCAGC 2 cut(s) 712, 819
LweI GCATC 3 cut(s) 574, 697, 719
MaeI CTAG 2 cut(s) 392, 770
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 2 cut(s) 460, 490
MalI GATC 5 cut(s) 356, 630, 682, 776, 780
MboI GATC 5 cut(s) 354, 628, 680, 774, 778
MboII GAAGA 4 cut(s) 72, 439, 613, 652
MhlI GDGCHC 2 cut(s) 278, 335
MluCI AATT 4 cut(s) 124, 193, 544, 573
Mly113I GGCGCC 1 cut(s) 648
MlyI GAGTC 2 cut(s) 222, 443
MmeI TCCRAC 2 cut(s) 306, 411
Mph1103I ATGCAT 1 cut(s) 712
MseI TTAA 3 cut(s) 62, 140, 399
MslI CAYNNNNRTG 2 cut(s) 338, 488
MspI CCGG 3 cut(s) 38, 272, 644
MspR9I CCNGG 3 cut(s) 233, 273, 645
MvaI CCWGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 828
MwoI GCNNNNNNNGC 1 cut(s) 731
NarI GGCGCC 1 cut(s) 648
NciI CCSGG 2 cut(s) 273, 645
NdeII GATC 5 cut(s) 354, 628, 680, 774, 778
NheI GCTAGC 1 cut(s) 391
NlaIV GGNNCC 5 cut(s) 36, 207, 277, 334, 649
NmeAIII GCCGAG 2 cut(s) 276, 445
NmuCI GTSAC 1 cut(s) 490
NsiI ATGCAT 1 cut(s) 712
OliI CACNNNNGTG 1 cut(s) 338
PaqCI CACCTGC 1 cut(s) 82
PfeI GAWTC 1 cut(s) 325
PkrI GCNGC 4 cut(s) 302, 727, 809, 827
Ple19I CGATCG 2 cut(s) 357, 781
PleI GAGTC 2 cut(s) 222, 442
PluTI GGCGCC 1 cut(s) 651
PpsI GAGTC 2 cut(s) 222, 442
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 5 cut(s) 36, 207, 277, 334, 649
PspPI GGNCC 4 cut(s) 35, 116, 467, 514
PstI CTGCAG 1 cut(s) 809
PstNI CAGNNNCTG 1 cut(s) 725
PvuI CGATCG 2 cut(s) 357, 781
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 2 cut(s) 338, 488
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 3 cut(s) 62, 140, 399
SatI GCNGC 4 cut(s) 301, 726, 808, 826
Sau3AI GATC 5 cut(s) 354, 628, 680, 774, 778
Sau96I GGNCC 4 cut(s) 35, 116, 467, 514
SchI GAGTC 2 cut(s) 222, 443
ScrFI CCNGG 3 cut(s) 233, 273, 645
SduI GDGCHC 2 cut(s) 278, 335
SfaNI GCATC 3 cut(s) 574, 697, 719
SfcI CTRYAG 1 cut(s) 805
SfoI GGCGCC 1 cut(s) 649
SinI GGWCC 2 cut(s) 116, 467
SmiMI CAYNNNNRTG 2 cut(s) 338, 488
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 4 cut(s) 124, 193, 544, 573
SsiI CCGC 5 cut(s) 20, 300, 417, 734, 826
SspDI GGCGCC 1 cut(s) 647
SspMI CTAG 2 cut(s) 392, 770
StyD4I CCNGG 3 cut(s) 231, 271, 643
TaaI ACNGT 4 cut(s) 150, 258, 795, 844
TaiI ACGT 1 cut(s) 58
TaqI TCGA 3 cut(s) 9, 237, 777
TaqII GACCGA 1 cut(s) 133
TasI AATT 4 cut(s) 124, 193, 544, 573
TatI WGTACW 1 cut(s) 363
TauI GCSGC 2 cut(s) 303, 828
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 3 cut(s) 62, 140, 399
Tru9I TTAA 3 cut(s) 62, 140, 399
TscAI CASTG 4 cut(s) 118, 250, 513, 586
TseFI GTSAC 1 cut(s) 490
TseI GCWGC 2 cut(s) 725, 807
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 2 cut(s) 472, 601
TspGWI ACGGA 4 cut(s) 250, 427, 459, 703
TspRI CASTG 4 cut(s) 118, 250, 513, 586
VpaK11BI GGWCC 2 cut(s) 116, 467
XapI RAATTY 1 cut(s) 573
XbaI TCTAGA 1 cut(s) 769
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 2 cut(s) 392, 770
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.