pycom07g17760

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004474
Physical Location & Seq
Reverse (-)
20280844 .. 20281283
440 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 381 bp
ATGTATTTGGCCTTGAGTCCATTTGAGAAATTCACACAGGTGACGTTCATACAGCACTTAACTGGCCGGCAGTACTCAGAGAAAGTTGCAGAGAATTGCATTGCCTCTTGGAAATCAGCCGGATTTTACAGCGGCGAAGAAGCCAAAGCCATTGAAACGTTCCTAGAGGTCTTCAAAGATCAAACCTTTCCTCCTGGCTCCTCTGTCTCTTTCGTTCAATCACCACCACATGACTCATTAACAGTAACTAGAACTTACGTACGCATATATATTCTTTTTTTATCTTTATATATTTTATTATACAACAGAAGTGTGAGATCGAATTTGAAACCTCGAGTGCTGAAACGAATGCTCTTAACTAACCAAGCTAAGAATCCCTGA

Protein Analysis

127

Amino Acids

14.51

Weight (kDa)

9.59

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 6 - 83 1.3e-28 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 132
AclI AACGTT 1 cut(s) 158
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 2 cut(s) 29, 322
AfaI GTAC 2 cut(s) 74, 261
AgsI TTSAA 4 cut(s) 155, 175, 218, 328
AjnI CCWGG 1 cut(s) 193
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 1 cut(s) 368
AluI AGCT 1 cut(s) 368
Alw26I GTCTC 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 333
AoxI GGCC 2 cut(s) 9, 64
ApoI RAATTY 2 cut(s) 29, 322
AsuHPI GGTGA 2 cut(s) 52, 213
AvaI CYCGRG 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 163
BciT130I CCWGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 2 cut(s) 164, 249
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 195
BmeT110I CYCGRG 1 cut(s) 333
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 34
BsaAI YACGTR 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bse118I RCCGGY 1 cut(s) 66
Bse1I ACTGG 1 cut(s) 67
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 195
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 1 cut(s) 67
BseRI GAGGAG 1 cut(s) 190
BshFI GGCC 2 cut(s) 11, 66
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 2 cut(s) 67, 120
BsiWI CGTACG 1 cut(s) 259
BsmAI GTCTC 1 cut(s) 211
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 2 cut(s) 11, 66
BsoBI CYCGRG 1 cut(s) 333
Bsp143I GATC 2 cut(s) 178, 317
BspACI CCGC 1 cut(s) 132
BspANI GGCC 2 cut(s) 11, 66
BspCNI CTCAG 1 cut(s) 89
BspLI GGNNCC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 66
BsrI ACTGG 1 cut(s) 67
BssAI RCCGGY 1 cut(s) 66
BssMI GATC 2 cut(s) 178, 317
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 244
BstBAI YACGTR 1 cut(s) 259
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 2 cut(s) 76, 369
BstKTI GATC 2 cut(s) 181, 320
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 2 cut(s) 178, 317
BstNI CCWGG 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 193
BstSNI TACGTA 1 cut(s) 259
BstV2I GAAGAC 1 cut(s) 163
BsuRI GGCC 2 cut(s) 11, 66
Cac8I GCNNGC 1 cut(s) 68
Cfr10I RCCGGY 1 cut(s) 66
Csp6I GTAC 2 cut(s) 73, 260
CviAII CATG 1 cut(s) 230
CviJI RGCY 7 cut(s) 11, 66, 119, 143, 149, 198, 368
CviKI_1 RGCY 7 cut(s) 11, 66, 119, 143, 149, 198, 368
CviQI GTAC 2 cut(s) 73, 260
DdeI CTNAG 2 cut(s) 76, 369
DpnI GATC 2 cut(s) 180, 319
DpnII GATC 2 cut(s) 178, 317
EaeI YGGCCR 1 cut(s) 64
Eco105I TACGTA 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 193
FaeI CATG 1 cut(s) 233
FaiI YATR 8 cut(s) 50, 231, 266, 268, 270, 289, 291, 301
FatI CATG 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 133
Fsp4HI GCNGC 1 cut(s) 133
FspBI CTAG 2 cut(s) 164, 249
GluI GCNGC 1 cut(s) 133
HaeIII GGCC 2 cut(s) 11, 66
HapII CCGG 2 cut(s) 67, 120
Hin1II CATG 1 cut(s) 233
HinfI GANTC 3 cut(s) 16, 233, 373
HpaII CCGG 2 cut(s) 67, 120
HphI GGTGA 2 cut(s) 52, 213
Hpy188I TCNGA 1 cut(s) 79
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 3 cut(s) 44, 158, 258
HpyCH4V TGCA 2 cut(s) 89, 99
HpyF3I CTNAG 2 cut(s) 76, 369
HpySE526I ACGT 3 cut(s) 44, 158, 258
Hsp92II CATG 1 cut(s) 233
KroI GCCGGC 1 cut(s) 66
KroNI GCCGGC 1 cut(s) 68
Kzo9I GATC 2 cut(s) 178, 317
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 6 cut(s) 23, 48, 80, 133, 180, 207
MaeI CTAG 2 cut(s) 164, 249
MaeII ACGT 3 cut(s) 44, 158, 258
MaeIII GTNAC 2 cut(s) 40, 244
MalI GATC 2 cut(s) 180, 319
MboI GATC 2 cut(s) 178, 317
MboII GAAGA 2 cut(s) 149, 163
MluCI AATT 3 cut(s) 29, 94, 322
MlyI GAGTC 2 cut(s) 25, 227
MnlI CCTC 5 cut(s) 115, 160, 201, 211, 342
MroNI GCCGGC 1 cut(s) 66
MseI TTAA 3 cut(s) 59, 239, 356
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 2 cut(s) 67, 120
MspR9I CCNGG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 1 cut(s) 195
NaeI GCCGGC 1 cut(s) 68
NdeII GATC 2 cut(s) 178, 317
NgoMIV GCCGGC 1 cut(s) 66
NlaIII CATG 1 cut(s) 233
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 40
OliI CACNNNNGTG 1 cut(s) 38
PaeR7I CTCGAG 1 cut(s) 333
PctI GAATGC 1 cut(s) 354
PdiI GCCGGC 1 cut(s) 68
PfeI GAWTC 1 cut(s) 373
Pfl23II CGTACG 1 cut(s) 259
PkrI GCNGC 1 cut(s) 134
PleI GAGTC 2 cut(s) 24, 227
PpsI GAGTC 2 cut(s) 24, 227
Ppu21I YACGTR 1 cut(s) 259
Psp1406I AACGTT 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspLI CGTACG 1 cut(s) 259
PspN4I GGNNCC 1 cut(s) 199
PspXI VCTCGAGB 1 cut(s) 333
RsaI GTAC 2 cut(s) 74, 261
RsaNI GTAC 2 cut(s) 73, 260
RseI CAYNNNNRTG 1 cut(s) 38
SaqAI TTAA 3 cut(s) 59, 239, 356
SatI GCNGC 1 cut(s) 133
Sau3AI GATC 2 cut(s) 178, 317
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 2 cut(s) 25, 227
ScrFI CCNGG 1 cut(s) 195
SetI ASST 8 cut(s) 42, 47, 161, 171, 188, 261, 334, 370
Sfr274I CTCGAG 1 cut(s) 333
SlaI CTCGAG 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 13, 333
SmoI CTYRAG 2 cut(s) 13, 333
SnaBI TACGTA 1 cut(s) 259
Sse9I AATT 3 cut(s) 29, 94, 322
SsiI CCGC 1 cut(s) 132
SspMI CTAG 2 cut(s) 164, 249
StyD4I CCNGG 1 cut(s) 193
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 3 cut(s) 47, 161, 261
TaqI TCGA 2 cut(s) 320, 334
TasI AATT 3 cut(s) 29, 94, 322
TatI WGTACW 1 cut(s) 72
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 3 cut(s) 59, 239, 356
Tru9I TTAA 3 cut(s) 59, 239, 356
TseFI GTSAC 1 cut(s) 40
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 1 cut(s) 37
XapI RAATTY 2 cut(s) 29, 322
XhoI CTCGAG 1 cut(s) 333
XspI CTAG 2 cut(s) 164, 249
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.