pycom07g21790

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002292
Physical Location & Seq
Forward (+)
23345984 .. 23346478
495 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGGAAACAAATACCCTTCAGTTGCAGCAGCCAAAAGACCCAATGCAATACTTCAAGTTCAAAAAGGGCAGAAAATCTGACGACGATCCATACGCCACGGTGCTGCTAGTTGTCGCTGTACTAGTGGCCACCACCACCTTTCAAGCCGGAATCAGCCCTCCGAACGGCGGTTTGCACTACACCACTGGATCAAATAAGAATGGGATTGGCTGTGGGAGCAATGGTTTAGTAGTTGTCTATATGTTTTTCAACACGATTGGATTTTCGGTTTCGCTTTTCATGATCGACTTCCTCACGCGCAATTTCCCTCTGCTGTTTCAGCTTCGATTTTGTATGGTGATGATGTACGGCACGTACACCGTAGCAGTTATCAGCACTGCACCGAAAAGCATGCTGCTCTGCATCACTTCGTCAACTCCATTATTTGTAGTGATTTTACAGTTGTGCTGTTGTACTAATTTTAAGGTGCCTTGGCAAAACCTAATAGGCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

165

Amino Acids

18.14

Weight (kDa)

8.96

Isoelectric Point (pI)

27.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 32 - 125 5.7e-09 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 466
AccII CGCG 1 cut(s) 298
AciI CCGC 1 cut(s) 168
AclWI GGATC 2 cut(s) 80, 196
AcoI YGGCCR 1 cut(s) 126
AfaI GTAC 4 cut(s) 120, 347, 356, 454
AfiI CCNNNNNNNGG 2 cut(s) 164, 167
AgsI TTSAA 4 cut(s) 55, 61, 143, 250
AhlI ACTAGT 1 cut(s) 121
AluBI AGCT 1 cut(s) 322
AluI AGCT 1 cut(s) 322
AlwI GGATC 2 cut(s) 80, 196
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 4 cut(s) 25, 28, 103, 394
AspLEI GCGC 1 cut(s) 300
AsuHPI GGTGA 1 cut(s) 349
BalI TGGCCA 1 cut(s) 128
BanI GGYRCC 1 cut(s) 466
BbvI GCAGC 4 cut(s) 37, 40, 90, 381
BceAI ACGGC 2 cut(s) 181, 364
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BcuI ACTAGT 1 cut(s) 121
BfaI CTAG 2 cut(s) 107, 122
BisI GCNGC 4 cut(s) 26, 29, 104, 395
BlsI GCNGC 4 cut(s) 27, 30, 105, 396
BmiI GGNNCC 1 cut(s) 468
BmsI GCATC 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 354
BsaJI CCNNGG 2 cut(s) 96, 470
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 167
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 226
BseDI CCNNGG 2 cut(s) 96, 470
BseLI CCNNNNNNNGG 2 cut(s) 164, 167
BseMI GCAATG 1 cut(s) 226
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 4 cut(s) 37, 40, 90, 381
BsgI GTGCAG 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 298
BshFI GGCC 1 cut(s) 128
BshNI GGYRCC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 147
BslI CCNNNNNNNGG 2 cut(s) 164, 167
BsnI GGCC 1 cut(s) 128
Bsp143I GATC 3 cut(s) 85, 188, 282
BspACI CCGC 1 cut(s) 168
BspANI GGCC 1 cut(s) 128
BspFNI CGCG 1 cut(s) 298
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 468
BspPI GGATC 2 cut(s) 80, 196
BspT107I GGYRCC 1 cut(s) 466
BsrDI GCAATG 1 cut(s) 226
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 96, 470
BssMI GATC 3 cut(s) 85, 188, 282
BssT1I CCWWGG 1 cut(s) 470
Bst4CI ACNGT 3 cut(s) 100, 361, 441
BstBAI YACGTR 1 cut(s) 354
BstC8I GCNNGC 1 cut(s) 392
BstDEI CTNAG 1 cut(s) 492
BstDSI CCRYGG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 3 cut(s) 88, 191, 285
BstMBI GATC 3 cut(s) 85, 188, 282
BstMWI GCNNNNNNNGC 2 cut(s) 216, 319
BstNSI RCATGY 1 cut(s) 394
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 4 cut(s) 37, 40, 90, 381
BsuRI GGCC 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 183, 375
Cac8I GCNNGC 1 cut(s) 392
CciI TCATGA 1 cut(s) 279
CfoI GCGC 1 cut(s) 300
Csp6I GTAC 4 cut(s) 119, 346, 355, 453
CviAII CATG 2 cut(s) 280, 391
CviJI RGCY 7 cut(s) 31, 128, 146, 156, 210, 322, 489
CviKI_1 RGCY 7 cut(s) 31, 128, 146, 156, 210, 322, 489
CviQI GTAC 4 cut(s) 119, 346, 355, 453
DdeI CTNAG 1 cut(s) 492
DpnI GATC 3 cut(s) 87, 190, 284
DpnII GATC 3 cut(s) 85, 188, 282
EaeI YGGCCR 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 470
EcoT14I CCWWGG 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 470
FaeI CATG 2 cut(s) 283, 394
FaiI YATR 6 cut(s) 91, 240, 242, 281, 335, 392
FatI CATG 2 cut(s) 279, 390
Fnu4HI GCNGC 4 cut(s) 26, 29, 104, 395
Fsp4HI GCNGC 4 cut(s) 26, 29, 104, 395
FspBI CTAG 2 cut(s) 107, 122
GlaI GCGC 1 cut(s) 299
GluI GCNGC 4 cut(s) 26, 29, 104, 395
HaeIII GGCC 1 cut(s) 128
HapII CCGG 1 cut(s) 147
HhaI GCGC 1 cut(s) 300
Hin1II CATG 2 cut(s) 283, 394
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 1 cut(s) 150
HpaII CCGG 1 cut(s) 147
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 2 cut(s) 357, 414
Hpy188I TCNGA 2 cut(s) 79, 162
Hpy188III TCNNGA 1 cut(s) 280
Hpy8I GTNNAC 2 cut(s) 357, 414
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 3 cut(s) 100, 361, 441
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 5 cut(s) 25, 46, 175, 380, 402
HpyF10VI GCNNNNNNNGC 2 cut(s) 216, 319
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 2 cut(s) 283, 394
HspAI GCGC 1 cut(s) 298
Kzo9I GATC 3 cut(s) 85, 188, 282
LmnI GCTCC 1 cut(s) 216
LpnPI CCDG 2 cut(s) 160, 171
Lsp1109I GCAGC 4 cut(s) 37, 40, 90, 381
LweI GCATC 1 cut(s) 411
MaeI CTAG 2 cut(s) 107, 122
MaeII ACGT 1 cut(s) 353
MalI GATC 3 cut(s) 87, 190, 284
MboI GATC 3 cut(s) 85, 188, 282
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 2 cut(s) 301, 457
MluNI TGGCCA 1 cut(s) 128
MnlI CCTC 3 cut(s) 168, 302, 318
Mox20I TGGCCA 1 cut(s) 128
MscI TGGCCA 1 cut(s) 128
MseI TTAA 1 cut(s) 462
Msp20I TGGCCA 1 cut(s) 128
MspI CCGG 1 cut(s) 147
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 2 cut(s) 216, 319
NdeII GATC 3 cut(s) 85, 188, 282
NlaIII CATG 2 cut(s) 283, 394
NlaIV GGNNCC 1 cut(s) 468
NspI RCATGY 1 cut(s) 394
PaeI GCATGC 1 cut(s) 394
PagI TCATGA 1 cut(s) 279
PfeI GAWTC 1 cut(s) 150
PkrI GCNGC 4 cut(s) 27, 30, 105, 396
Ppu21I YACGTR 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 468
RsaI GTAC 4 cut(s) 120, 347, 356, 454
RsaNI GTAC 4 cut(s) 119, 346, 355, 453
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 4 cut(s) 26, 29, 104, 395
Sau3AI GATC 3 cut(s) 85, 188, 282
SetI ASST 5 cut(s) 140, 324, 356, 468, 483
SfaNI GCATC 1 cut(s) 411
SpeI ACTAGT 1 cut(s) 121
SphI GCATGC 1 cut(s) 394
Sse9I AATT 2 cut(s) 301, 457
SsiI CCGC 1 cut(s) 168
SspMI CTAG 2 cut(s) 107, 122
StyI CCWWGG 1 cut(s) 470
TaaI ACNGT 3 cut(s) 100, 361, 441
TaiI ACGT 1 cut(s) 356
TaqI TCGA 2 cut(s) 285, 325
TasI AATT 2 cut(s) 301, 457
TatI WGTACW 2 cut(s) 118, 452
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 2 cut(s) 190, 382
TseI GCWGC 4 cut(s) 25, 28, 103, 394
TspDTI ATGAA 1 cut(s) 268
TspRI CASTG 2 cut(s) 190, 382
XceI RCATGY 1 cut(s) 394
XspI CTAG 2 cut(s) 107, 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.