pycom07g27830

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002642
Physical Location & Seq
Forward (+)
27387071 .. 27388525
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1353 bp
ATGCTGGTGCAGTCCATTTGGTGTCGCACTTCACCTTCACTTTCATCCACAGCCAGCTTCTTCACAATCACTCCACTCGCTCTCTTCTTTCCGAGCTGCCTCAAACACCCCGGGACATTCAAGTTCTACGCTTCTTTCACCCACTCGAATCGCATCAAGACCGGGAAAGAAGTCAAATTTACGTCCCCCAATGACGAAAACACTTCAGTTCCCAGCTATCCAATTGCTCAAACCACTGGAGAGGAACTTTCGGGCGAATCATACAACAATTTGATAAGTGGGTTCTGCAGGGCAGGGCATATCGACAAGGCCATGGCTGTTCTTGCAGAAATGGAAGCTCTCGGCGTTCGACCCAATTCGGTGTCTTACGCTTATTTGATTGACGGTCTTGGAAGCAATGGCAGGACTTTGGAGGCCGATGTGTTGTTTCAGGAAATGATATGTTCTGGGTTGAGGCCACGACTCAGGGTTTACAATGTTATGCTCAGAGGGTGTTTGAAGAAAGGCCTCTTAGAACTTGCTACTAGAGTTTTGGCAGTAATGGGCTATTTGGGTGTGGAGAAAAATGAAGAGACGTATGAGATTCTTCTTGATTACTATGTCAGTGCCGGGAGGTTGGAAGATACTTGGTCAATGATTAATGAGATGAAGCGGAAGCGGTTTCGGTTGAGCTCGTTTGTGTATAGCAAGGTTATTGGTCTTTACAGGGACAATGGGATGTGGAAGAAGGCAATGGACATTGTGGGAGAGATAAGGGAAATGGGGATGGCATTGGATAAACAGATTTTCAACAGCATTATTGATACGTTTGGGAAATATGGCGAATTGGACGAAGCTTTGGAAGTGTTTGATAAAATGAAACAAGAAGGTCTGAAGCCTGATATAACGACGTTTAATTCGTTGATAAGGTGGTATTGTAAAGCAGGGAATATAAGCAAGGCTCTTGAGTTGTTTACCGAGATGCAGGAACAGGGGTTGTATCCTGATCCAAAGATCTTTGTCACTGTTATTAGCCGATTAGGGGAGCAGGGGAAGTGGGATATGATCCAGAAGACTTTTGAGAATATGAAGCGCCGAGGGCATAAGAAAAGTGGGATAATTTATGCTGTTTTGGTTGATATTTATGGGCAGTATGGAAAATTTCAGGATGCTCAAGAGTGCATATCTGCCCTAAAGGTGGAAGGTCTTATTCCTTCAGCCAGTATGTTTTGTGTCTTAGCAAATGCCTACGCTCAACAGGGATTATGTCATCAGACGGTTAAGGTGCTTCAGCTCATGGAAGCTGAGGGAATCGAACCAAACGTCATAATGCTGAATGTGCTGATCGATGCGGTTATTCGGTGTTGCTGGTAG

Protein Analysis

451

Amino Acids

50.75

Weight (kDa)

7.46

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 73 - 218 7.7e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 80 - 131 5.4e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 87 - 130 2e-13 PPR repeat family
PPR PF01535 87 - 116 1.2e-07 PPR repeat
PPR_1 PF12854 87 - 112 8e-09 PPR repeat
PPR_2 PF13041 157 - 198 3.6e-06 PPR repeat family
TPR_24 PF23276 164 - 285 5.6e-11 Fungal tetratrico peptide repeats
PPR_2 PF13041 189 - 232 4.2e-07 PPR repeat family
PPR_long PF17177 213 - 328 6.4e-07 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 237 - 302 2.7e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 255 - 287 1.5e-06 PPR repeat
PPR_3 PF13812 262 - 305 6.2e-09 Pentatricopeptide repeat domain
PPR PF01535 262 - 291 8.1e-07 PPR repeat
PPR_2 PF13041 262 - 303 1e-11 PPR repeat family
PPR_3 PF13812 282 - 336 1.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 290 - 322 1.6e-11 PPR repeat
PPR_2 PF13041 293 - 329 2.3e-11 PPR repeat family
PPR PF01535 297 - 326 1.8e-09 PPR repeat
TPR_24 PF23276 334 - 447 1.6e-06 Fungal tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 652, 658, 1331
AclWI GGATC 2 cut(s) 980, 1039
AcsI RAATTY 2 cut(s) 176, 1139
AcuI CTGAAG 4 cut(s) 189, 893, 1179, 1253
AfiI CCNNNNNNNGG 2 cut(s) 1021, 1177
AgsI TTSAA 3 cut(s) 121, 499, 790
AluBI AGCT 8 cut(s) 57, 96, 216, 338, 672, 836, 1273, 1283
AluI AGCT 8 cut(s) 57, 96, 216, 338, 672, 836, 1273, 1283
Alw21I GWGCWC 1 cut(s) 674
Alw26I GTCTC 1 cut(s) 566
AlwI GGATC 2 cut(s) 980, 1039
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 4 cut(s) 309, 414, 455, 505
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 2 cut(s) 176, 1139
ArsI GACNNNNNNTTYG 4 cut(s) 880, 912, 1287, 1319
AseI ATTAAT 1 cut(s) 639
AspLEI GCGC 1 cut(s) 1074
AsuC2I CCSGG 4 cut(s) 111, 112, 163, 610
AsuHPI GGTGA 2 cut(s) 24, 130
AvaI CYCGRG 1 cut(s) 110
BanII GRGCYC 1 cut(s) 674
BbsI GAAGAC 1 cut(s) 1058
Bbv12I GWGCWC 1 cut(s) 674
BbvCI CCTCAGC 1 cut(s) 1284
BbvI GCAGC 1 cut(s) 83
BccI CCATC 1 cut(s) 760
BciVI GTATCC 1 cut(s) 990
BcnI CCSGG 4 cut(s) 111, 112, 163, 610
BcoDI GTCTC 1 cut(s) 566
BfaI CTAG 1 cut(s) 525
BfmI CTRYAG 1 cut(s) 286
BfoI RGCGCY 1 cut(s) 1075
BfuI GTATCC 1 cut(s) 990
BglII AGATCT 1 cut(s) 993
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
Bme1390I CCNGG 4 cut(s) 111, 112, 163, 610
BmeT110I CYCGRG 1 cut(s) 110
BmrFI CCNGG 4 cut(s) 111, 112, 163, 610
BmsI GCATC 4 cut(s) 162, 951, 1138, 1318
BpiI GAAGAC 1 cut(s) 1058
BpmI CTGGAG 1 cut(s) 258
Bpu10I CCTNAGC 1 cut(s) 1284
BpuEI CTTGAG 2 cut(s) 965, 1137
BpuMI CCSGG 4 cut(s) 111, 112, 163, 610
Bsa29I ATCGAT 1 cut(s) 1326
BsaJI CCNNGG 4 cut(s) 109, 110, 312, 1075
BsaXI ACNNNNNCTCC 2 cut(s) 55, 85
Bsc4I CCNNNNNNNGG 2 cut(s) 1021, 1177
Bse1I ACTGG 2 cut(s) 241, 1200
Bse3DI GCAATG 2 cut(s) 403, 738
BseCI ATCGAT 1 cut(s) 1326
BseDI CCNNGG 4 cut(s) 109, 110, 312, 1075
BseGI GGATG 4 cut(s) 44, 723, 771, 1153
BseLI CCNNNNNNNGG 2 cut(s) 1021, 1177
BseMI GCAATG 2 cut(s) 403, 738
BseMII CTCAG 3 cut(s) 478, 499, 1275
BseNI ACTGG 2 cut(s) 241, 1200
BseXI GCAGC 1 cut(s) 83
BseYI CCCAGC 1 cut(s) 212
BsgI GTGCAG 1 cut(s) 29
BshFI GGCC 4 cut(s) 311, 416, 457, 507
BshVI ATCGAT 1 cut(s) 1326
BsiHKAI GWGCWC 1 cut(s) 674
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 3 cut(s) 111, 162, 609
BslFI GGGAC 3 cut(s) 127, 169, 722
BslI CCNNNNNNNGG 2 cut(s) 1021, 1177
BsmAI GTCTC 1 cut(s) 566
BsmBI CGTCTC 1 cut(s) 566
BsmFI GGGAC 3 cut(s) 127, 169, 722
BsnI GGCC 4 cut(s) 311, 416, 457, 507
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 674
Bsp143I GATC 4 cut(s) 985, 993, 1044, 1323
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 3 cut(s) 652, 658, 1331
BspANI GGCC 4 cut(s) 311, 416, 457, 507
BspCNI CTCAG 3 cut(s) 477, 498, 1276
BspDI ATCGAT 1 cut(s) 1326
BspMAI CTGCAG 1 cut(s) 290
BspPI GGATC 2 cut(s) 980, 1039
BsrDI GCAATG 2 cut(s) 403, 738
BsrI ACTGG 2 cut(s) 241, 1200
BssECI CCNNGG 4 cut(s) 109, 110, 312, 1075
BssMI GATC 4 cut(s) 985, 993, 1044, 1323
BssT1I CCWWGG 1 cut(s) 312
Bst4CI ACNGT 3 cut(s) 386, 1006, 1258
Bst6I CTCTTC 2 cut(s) 89, 564
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 5 cut(s) 464, 485, 511, 1216, 1284
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 4 cut(s) 44, 723, 771, 1153
BstH2I RGCGCY 1 cut(s) 1075
BstHHI GCGC 1 cut(s) 1074
BstKTI GATC 4 cut(s) 988, 996, 1047, 1326
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 4 cut(s) 985, 993, 1044, 1323
BstMWI GCNNNNNNNGC 3 cut(s) 323, 1078, 1318
BstSCI CCNGG 4 cut(s) 109, 110, 161, 608
BstSFI CTRYAG 1 cut(s) 286
BstV1I GCAGC 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 1058
BstX2I RGATCY 1 cut(s) 993
BstYI RGATCY 1 cut(s) 993
Bsu15I ATCGAT 1 cut(s) 1326
BsuI GTATCC 1 cut(s) 990
BsuRI GGCC 4 cut(s) 311, 416, 457, 507
BsuTUI ATCGAT 1 cut(s) 1326
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 4 cut(s) 44, 723, 771, 1153
BtsIMutI CAGTG 3 cut(s) 234, 610, 1002
Cac8I GCNNGC 1 cut(s) 55
CfoI GCGC 1 cut(s) 1074
Cfr9I CCCGGG 1 cut(s) 110
ClaI ATCGAT 1 cut(s) 1326
CviAII CATG 2 cut(s) 313, 1276
DdeI CTNAG 5 cut(s) 464, 485, 511, 1216, 1284
DpnI GATC 4 cut(s) 987, 995, 1046, 1325
DpnII GATC 4 cut(s) 985, 993, 1044, 1323
Eam1104I CTCTTC 2 cut(s) 89, 564
EarI CTCTTC 2 cut(s) 89, 564
Ecl136II GAGCTC 1 cut(s) 672
Eco130I CCWWGG 1 cut(s) 312
Eco147I AGGCCT 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 674
Eco53kI GAGCTC 1 cut(s) 672
Eco57I CTGAAG 4 cut(s) 189, 893, 1179, 1253
Eco88I CYCGRG 1 cut(s) 110
EcoICRI GAGCTC 1 cut(s) 672
EcoT14I CCWWGG 1 cut(s) 312
EcoT38I GRGCYC 1 cut(s) 674
ErhI CCWWGG 1 cut(s) 312
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 2 cut(s) 316, 1279
FaqI GGGAC 3 cut(s) 127, 169, 722
FatI CATG 2 cut(s) 312, 1275
Fnu4HI GCNGC 1 cut(s) 97
FokI GGATG 4 cut(s) 31, 730, 778, 1160
FriOI GRGCYC 1 cut(s) 674
Fsp4HI GCNGC 1 cut(s) 97
FspBI CTAG 1 cut(s) 525
GlaI GCGC 1 cut(s) 1073
GluI GCNGC 1 cut(s) 97
GsaI CCCAGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 1075
HaeIII GGCC 4 cut(s) 311, 416, 457, 507
HapII CCGG 3 cut(s) 111, 162, 609
HhaI GCGC 1 cut(s) 1074
Hin1II CATG 2 cut(s) 316, 1279
Hin6I GCGC 1 cut(s) 1072
HinP1I GCGC 1 cut(s) 1072
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 5 cut(s) 148, 257, 462, 583, 1290
HpaII CCGG 3 cut(s) 111, 162, 609
HphI GGTGA 2 cut(s) 24, 130
Hpy166II GTNNAC 2 cut(s) 472, 954
Hpy188I TCNGA 4 cut(s) 93, 488, 873, 1254
Hpy188III TCNNGA 8 cut(s) 157, 431, 590, 944, 983, 1048, 1145, 1154
Hpy8I GTNNAC 2 cut(s) 472, 954
Hpy99I CGWCG 1 cut(s) 892
HpyAV CCTTC 5 cut(s) 45, 721, 860, 1175, 1203
HpyCH4III ACNGT 3 cut(s) 386, 1006, 1258
HpyCH4IV ACGT 5 cut(s) 182, 575, 806, 890, 1302
HpyCH4V TGCA 5 cut(s) 10, 288, 326, 964, 1161
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 1078, 1318
HpyF3I CTNAG 5 cut(s) 464, 485, 511, 1216, 1284
HpySE526I ACGT 5 cut(s) 182, 575, 806, 890, 1302
Hsp92II CATG 2 cut(s) 316, 1279
HspAI GCGC 1 cut(s) 1072
Kzo9I GATC 4 cut(s) 985, 993, 1044, 1323
LmnI GCTCC 1 cut(s) 1024
Lsp1109I GCAGC 1 cut(s) 83
LweI GCATC 4 cut(s) 162, 951, 1138, 1318
MaeI CTAG 1 cut(s) 525
MaeII ACGT 5 cut(s) 182, 575, 806, 890, 1302
MaeIII GTNAC 1 cut(s) 1000
MalI GATC 4 cut(s) 987, 995, 1046, 1325
MboI GATC 4 cut(s) 985, 993, 1044, 1323
MboII GAAGA 8 cut(s) 52, 76, 511, 578, 581, 632, 736, 1063
MfeI CAATTG 1 cut(s) 222
MflI RGATCY 1 cut(s) 993
MhlI GDGCHC 1 cut(s) 674
MluCI AATT 8 cut(s) 176, 222, 268, 355, 824, 895, 1098, 1139
MlyI GAGTC 1 cut(s) 456
MmeI TCCRAC 1 cut(s) 597
MnlI CCTC 9 cut(s) 110, 235, 406, 447, 482, 518, 606, 1070, 1279
MseI TTAA 3 cut(s) 639, 894, 1260
MspI CCGG 3 cut(s) 111, 162, 609
MspR9I CCNGG 4 cut(s) 111, 112, 163, 610
MunI CAATTG 1 cut(s) 222
MwoI GCNNNNNNNGC 3 cut(s) 323, 1078, 1318
NciI CCSGG 4 cut(s) 111, 112, 163, 610
NcoI CCATGG 1 cut(s) 312
NdeII GATC 4 cut(s) 985, 993, 1044, 1323
NlaIII CATG 2 cut(s) 316, 1279
NmeAIII GCCGAG 2 cut(s) 321, 1100
NmuCI GTSAC 1 cut(s) 1000
PceI AGGCCT 1 cut(s) 507
PcsI WCGNNNNNNNCGW 2 cut(s) 896, 1299
PfeI GAWTC 4 cut(s) 148, 257, 583, 1290
PkrI GCNGC 1 cut(s) 98
PleI GAGTC 1 cut(s) 456
PpsI GAGTC 1 cut(s) 456
PshBI ATTAAT 1 cut(s) 639
Psp124BI GAGCTC 1 cut(s) 674
PspFI CCCAGC 1 cut(s) 212
PstI CTGCAG 1 cut(s) 290
PsuI RGATCY 1 cut(s) 993
SacI GAGCTC 1 cut(s) 674
SaqAI TTAA 3 cut(s) 639, 894, 1260
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 4 cut(s) 985, 993, 1044, 1323
SchI GAGTC 1 cut(s) 456
ScrFI CCNGG 4 cut(s) 111, 112, 163, 610
SduI GDGCHC 1 cut(s) 674
SfaNI GCATC 4 cut(s) 162, 951, 1138, 1318
SfcI CTRYAG 1 cut(s) 286
SmaI CCCGGG 1 cut(s) 112
SmlI CTYRAG 2 cut(s) 944, 1152
SmoI CTYRAG 2 cut(s) 944, 1152
Sse9I AATT 8 cut(s) 176, 222, 268, 355, 824, 895, 1098, 1139
SseBI AGGCCT 1 cut(s) 507
SsiI CCGC 3 cut(s) 652, 658, 1331
SspMI CTAG 1 cut(s) 525
SstI GAGCTC 1 cut(s) 674
StuI AGGCCT 1 cut(s) 507
StyD4I CCNGG 4 cut(s) 109, 110, 161, 608
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 3 cut(s) 386, 1006, 1258
TaiI ACGT 5 cut(s) 185, 578, 809, 893, 1305
TaqI TCGA 5 cut(s) 146, 303, 349, 1293, 1326
TasI AATT 8 cut(s) 176, 222, 268, 355, 824, 895, 1098, 1139
TfiI GAWTC 4 cut(s) 148, 257, 583, 1290
Tru1I TTAA 3 cut(s) 639, 894, 1260
Tru9I TTAA 3 cut(s) 639, 894, 1260
TscAI CASTG 3 cut(s) 241, 610, 1009
TseFI GTSAC 1 cut(s) 1000
TseI GCWGC 1 cut(s) 96
Tsp45I GTSAC 1 cut(s) 1000
TspDTI ATGAA 5 cut(s) 33, 582, 662, 872, 1082
TspMI CCCGGG 1 cut(s) 110
TspRI CASTG 3 cut(s) 241, 610, 1009
VspI ATTAAT 1 cut(s) 639
XapI RAATTY 2 cut(s) 176, 1139
XmaI CCCGGG 1 cut(s) 110
XspI CTAG 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.