pycom08g15280

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003160
Physical Location & Seq
Reverse (-)
14903896 .. 14904912
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGTTGTTGGGGAAGAGACCAAGGCCTCCAATGAAGAGGACCACAAGCATGTCAGAAATCACCTTCGATCTGAACACCATTAGTACTGGGGCACCTCATTCTCAACCGTCCGATCCCAACAACCCTTATCAGCCTCCGGCCTTATGGGGTAGTGGTGGTGGTGGTGGTGGGCTTGATGGGTTGGATCAACGGCTTATGATGAAGTTGCCTTCACCATCATCAGCATCGCCAAGAAACCACAGAAGAAATTCTGCTGATTTTGGAGAAACTGCCCACTTCTTGAAGGCTTGTGGTCTCTGCAAACGCCGACTTATTCCCGGCCGCGATATTTACATGTACAGAGGTGACACAGCTTTCTGCAGTCTAGAGTGCCGGCAGCAACAGATGAATCTTGATGAGAGGAAGGACAAGTGTTCTTCCATCGCTGGACACCAAGTCTCCGCCAAAGGCGAAACCATGGTCGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

157

Amino Acids

17.06

Weight (kDa)

9.43

Isoelectric Point (pI)

62.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 87 - 135 1.8e-26 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 91
AccII CGCG 1 cut(s) 324
AciI CCGC 2 cut(s) 322, 441
AclWI GGATC 2 cut(s) 107, 192
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 1 cut(s) 247
AfaI GTAC 2 cut(s) 85, 338
AflIII ACRYGT 1 cut(s) 333
AgsI TTSAA 1 cut(s) 283
AhdI GACNNNNNGTC 1 cut(s) 434
AluBI AGCT 1 cut(s) 353
AluI AGCT 1 cut(s) 353
Alw26I GTCTC 3 cut(s) 10, 299, 442
AlwI GGATC 2 cut(s) 107, 192
AoxI GGCC 3 cut(s) 23, 138, 319
ApeKI GCWGC 1 cut(s) 376
ApoI RAATTY 1 cut(s) 247
ArsI GACNNNNNNTTYG 1 cut(s) 444
Asp700I GAANNNNTTC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 39
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 3 cut(s) 52, 204, 356
AvaII GGWCC 1 cut(s) 39
BaeGI GKGCMC 1 cut(s) 94
BanI GGYRCC 1 cut(s) 91
BbvI GCAGC 1 cut(s) 388
BccI CCATC 3 cut(s) 170, 223, 428
BceAI ACGGC 1 cut(s) 206
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 3 cut(s) 10, 299, 442
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 1 cut(s) 358
BisI GCNGC 2 cut(s) 322, 377
BlsI GCNGC 2 cut(s) 323, 378
BmcAI AGTACT 1 cut(s) 85
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 39
BmeRI GACNNNNNGTC 1 cut(s) 434
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 318
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 1 cut(s) 233
BmuI ACTGGG 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 318
BsaI GGTCTC 2 cut(s) 10, 299
BsaJI CCNNGG 2 cut(s) 20, 456
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 1 cut(s) 91
BseDI CCNNGG 2 cut(s) 20, 456
BseNI ACTGG 1 cut(s) 91
BseSI GKGCMC 1 cut(s) 94
BseX3I CGGCCG 1 cut(s) 319
BseXI GCAGC 1 cut(s) 388
Bsh1236I CGCG 1 cut(s) 324
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 3 cut(s) 25, 140, 321
BshNI GGYRCC 1 cut(s) 91
BsiEI CGRYCG 1 cut(s) 322
BsiSI CCGG 3 cut(s) 137, 318, 373
BsmAI GTCTC 3 cut(s) 10, 299, 442
BsnI GGCC 3 cut(s) 25, 140, 321
Bso31I GGTCTC 2 cut(s) 10, 299
Bsp1286I GDGCHC 1 cut(s) 94
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 3 cut(s) 67, 112, 184
Bsp19I CCATGG 1 cut(s) 456
BspACI CCGC 2 cut(s) 322, 441
BspANI GGCC 3 cut(s) 25, 140, 321
BspFNI CGCG 1 cut(s) 324
BspLI GGNNCC 1 cut(s) 93
BspMAI CTGCAG 1 cut(s) 362
BspPI GGATC 2 cut(s) 107, 192
BspT107I GGYRCC 1 cut(s) 91
BspTNI GGTCTC 2 cut(s) 10, 299
BsrFI RCCGGY 1 cut(s) 372
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 2 cut(s) 20, 456
BssMI GATC 3 cut(s) 67, 112, 184
BssT1I CCWWGG 2 cut(s) 20, 456
Bst4CI ACNGT 1 cut(s) 108
Bst6I CTCTTC 2 cut(s) 8, 29
BstAUI TGTACA 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 374
BstDSI CCRYGG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 324
BstKTI GATC 3 cut(s) 70, 115, 187
BstMAI GTCTC 3 cut(s) 10, 299, 442
BstMBI GATC 3 cut(s) 67, 112, 184
BstMCI CGRYCG 1 cut(s) 322
BstNSI RCATGY 2 cut(s) 52, 337
BstSCI CCNGG 1 cut(s) 316
BstSFI CTRYAG 1 cut(s) 358
BstSLI GKGCMC 1 cut(s) 94
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 1 cut(s) 388
BstZI CGGCCG 1 cut(s) 319
BsuRI GGCC 3 cut(s) 25, 140, 321
BtgI CCRYGG 1 cut(s) 456
BtgZI GCGATG 2 cut(s) 210, 406
Cac8I GCNNGC 1 cut(s) 374
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 39
Csp6I GTAC 2 cut(s) 84, 337
CviAII CATG 3 cut(s) 49, 334, 457
CviJI RGCY 8 cut(s) 25, 133, 140, 172, 193, 287, 321, 353
CviKI_1 RGCY 8 cut(s) 25, 133, 140, 172, 193, 287, 321, 353
CviQI GTAC 2 cut(s) 84, 337
DpnI GATC 3 cut(s) 69, 114, 186
DpnII GATC 3 cut(s) 67, 112, 184
DriI GACNNNNNGTC 1 cut(s) 434
EaeI YGGCCR 1 cut(s) 319
EagI CGGCCG 1 cut(s) 319
Eam1104I CTCTTC 2 cut(s) 8, 29
Eam1105I GACNNNNNGTC 1 cut(s) 434
EarI CTCTTC 2 cut(s) 8, 29
EciI GGCGGA 1 cut(s) 430
EclXI CGGCCG 1 cut(s) 319
Eco130I CCWWGG 2 cut(s) 20, 456
Eco147I AGGCCT 1 cut(s) 25
Eco31I GGTCTC 2 cut(s) 10, 299
Eco47I GGWCC 1 cut(s) 39
Eco52I CGGCCG 1 cut(s) 319
EcoT14I CCWWGG 2 cut(s) 20, 456
ErhI CCWWGG 2 cut(s) 20, 456
FaeI CATG 3 cut(s) 52, 337, 460
FaiI YATR 5 cut(s) 50, 145, 197, 335, 458
FatI CATG 3 cut(s) 48, 333, 456
Fnu4HI GCNGC 2 cut(s) 322, 377
Fsp4HI GCNGC 2 cut(s) 322, 377
FspBI CTAG 1 cut(s) 365
GluI GCNGC 2 cut(s) 322, 377
HaeIII GGCC 3 cut(s) 25, 140, 321
HapII CCGG 3 cut(s) 137, 318, 373
Hin1II CATG 3 cut(s) 52, 337, 460
HinfI GANTC 1 cut(s) 388
HpaII CCGG 3 cut(s) 137, 318, 373
HphI GGTGA 3 cut(s) 52, 204, 356
Hpy188I TCNGA 3 cut(s) 55, 72, 112
Hpy188III TCNNGA 3 cut(s) 280, 365, 392
HpyAV CCTTC 4 cut(s) 73, 219, 277, 397
HpyCH4III ACNGT 1 cut(s) 108
HpyCH4V TGCA 2 cut(s) 300, 360
Hsp92II CATG 3 cut(s) 52, 337, 460
KroI GCCGGC 1 cut(s) 372
KroNI GCCGGC 1 cut(s) 374
Kzo9I GATC 3 cut(s) 67, 112, 184
LpnPI CCDG 5 cut(s) 72, 150, 331, 386, 411
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 1 cut(s) 233
MaeI CTAG 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 344
MalI GATC 3 cut(s) 69, 114, 186
MboI GATC 3 cut(s) 67, 112, 184
MboII GAAGA 4 cut(s) 25, 46, 255, 408
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 6 cut(s) 30, 36, 105, 144, 335, 393
MroNI GCCGGC 1 cut(s) 372
MroXI GAANNNNTTC 1 cut(s) 247
MslI CAYNNNNRTG 1 cut(s) 47
MspI CCGG 3 cut(s) 137, 318, 373
MspR9I CCNGG 1 cut(s) 318
MvnI CGCG 1 cut(s) 324
NaeI GCCGGC 1 cut(s) 374
NciI CCSGG 1 cut(s) 318
NcoI CCATGG 1 cut(s) 456
NdeII GATC 3 cut(s) 67, 112, 184
NgoMIV GCCGGC 1 cut(s) 372
NlaIII CATG 3 cut(s) 52, 337, 460
NlaIV GGNNCC 1 cut(s) 93
NmuCI GTSAC 1 cut(s) 344
NspI RCATGY 2 cut(s) 52, 337
PceI AGGCCT 1 cut(s) 25
PciI ACATGT 1 cut(s) 333
PdiI GCCGGC 1 cut(s) 374
PdmI GAANNNNTTC 1 cut(s) 247
PfeI GAWTC 1 cut(s) 388
PkrI GCNGC 2 cut(s) 323, 378
PscI ACATGT 1 cut(s) 333
PspN4I GGNNCC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 39
PstI CTGCAG 1 cut(s) 362
RsaI GTAC 2 cut(s) 85, 338
RsaNI GTAC 2 cut(s) 84, 337
RseI CAYNNNNRTG 1 cut(s) 47
SatI GCNGC 2 cut(s) 322, 377
Sau3AI GATC 3 cut(s) 67, 112, 184
Sau96I GGNCC 1 cut(s) 39
ScaI AGTACT 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 318
SduI GDGCHC 1 cut(s) 94
SetI ASST 4 cut(s) 65, 97, 346, 355
SfaNI GCATC 1 cut(s) 233
SfcI CTRYAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 47
Sse9I AATT 1 cut(s) 247
SseBI AGGCCT 1 cut(s) 25
SsiI CCGC 2 cut(s) 322, 441
SspMI CTAG 1 cut(s) 365
StuI AGGCCT 1 cut(s) 25
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 2 cut(s) 20, 456
TaaI ACNGT 1 cut(s) 108
TaqI TCGA 1 cut(s) 66
TasI AATT 1 cut(s) 247
TatI WGTACW 2 cut(s) 83, 336
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 1 cut(s) 388
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 1 cut(s) 376
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 3 cut(s) 47, 215, 401
VpaK11BI GGWCC 1 cut(s) 39
XapI RAATTY 1 cut(s) 247
XbaI TCTAGA 1 cut(s) 364
XceI RCATGY 2 cut(s) 52, 337
XmnI GAANNNNTTC 1 cut(s) 247
XspI CTAG 1 cut(s) 365
ZrmI AGTACT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.