pycom08g17510

CRT-like, chloroquine-resistance transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003123
Physical Location & Seq
Reverse (-)
17714132 .. 17719496
5365 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1305 bp
ATGGGATATTCAATGACCACCACGTCGTCGTTTCACCACTTCTTCTCCGCCCCTTTTCACGCCCGGCACTGCACCGTCAAAAATGGCGCGATCCCCTTCCCTTCTCTCTCCTCCTCCGCAATGCCGCTCTTTCAAAGGCCTCGACTCTCTCTCCTCCGCCTCCCTCAAAACGCACCGCTTCACCACACCTCTGAACGCTCCTCCCGCAGTCCCTCACCGGCAACTCGCAATGTAAAGGCCCGAGCATCGCTCCCAGAGCCGCCCTCGTCCTCCTCCTCCACCTCCACCATTACAACACGCGTCGTTTTGAGCTCCGCATTGACCATAGCTCTGGCCGTCGCCAACCGCGTGTTCTACAAGCTGGCCCTCGTCCCCATGAAAAGCCACCCTTTCTTCTTGGCCCAATTCACAACCTTCGGCTACGTGGTGATATATTTTTCGATATTGGTCGTACGGTACCGTTCTGGGGTTGTTACCGACGAAATGATAGGTCTTCCCAAGTCACGGTTTGCCGCCATTGGTGCCCTAGAAGCCCTTGGAGTTGCTTCTGGAATGGCTGCTGCAGCCATGCTTCCCGGACCGGCCATACCCATATTGAGTCAGACGTTTTTGGTGTGGCAGCTGAGTTTTTCTGCCCTTCTCTTGGGGAGAACATACACATTTAATCAGATTGTTGGCTGCATACTGGTAGCTACAGGTGTAGCAGCGGCTGTTTCAAGTGGCTCTGATTCTGGTCAGATGCTATCTGGAGTTGAGTCTATATGGCCAGCACTAATGATTGCTTCAAGTGCTTTTCAAGCCGGTGCATCTATAATCAAGGAGTTTGTCTTTGTTGATGCTGCAGCCCACCTTAAGGAAAAGTCACTTGACATATTTGTCGTCAATTCTTTTGGATCTGGATTTCAGGCTCTTTTTGTTCTTCTGTTTCTACCTTTCTTGTCAAACCTGAAAGGCATACCATTTGCGCAACTTCCTTCATATCTTAAAGATGGTGCTTGTTGCTTTCTGAATATTGGAGCTGATACAACAGGTTGCAATGGGGCTCCGTTACTACCATTGCTGTATATAGCTACCAATCTGTTTTTCAACGTATCATTGCTGAATGTCGTAAAGATTTCCTCTGCAGTTGTTGCTTCTCTCATCGTGATGTTGTCAGTGCCAATATCAATTTATGTTCTTTCCCTTCCCTTGCCGTATATGGAAGGAGGTTCAACTTTGAGCCCCTTTTTCCTCTTCGGTAGTGCGGTTCTTGTGTTGGGTCTTACTCTGTACAGCATACCCCAGCCTGCCAAGGATGTGCTGTGA

Protein Analysis

435

Amino Acids

46.14

Weight (kDa)

9.55

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 102 - 424 6.4e-34 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 22, 875
Acc16I TGCGCA 1 cut(s) 966
Acc65I GGTACC 1 cut(s) 456
AccB1I GGYRCC 2 cut(s) 456, 521
AccBSI CCGCTC 1 cut(s) 127
AccII CGCG 3 cut(s) 89, 300, 348
AclWI GGATC 2 cut(s) 85, 901
AcoI YGGCCR 3 cut(s) 333, 582, 764
AfaI GTAC 3 cut(s) 453, 458, 1271
AfiI CCNNNNNNNGG 4 cut(s) 466, 504, 643, 853
AflII CTTAAG 1 cut(s) 851
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 7 cut(s) 12, 134, 717, 786, 797, 1087, 1212
AjiI CACGTC 1 cut(s) 24
AjuI GAANNNNNNNTTGG 4 cut(s) 335, 367, 1067, 1099
AluBI AGCT 7 cut(s) 312, 329, 361, 622, 692, 1019, 1070
AluI AGCT 7 cut(s) 312, 329, 361, 622, 692, 1019, 1070
Alw21I GWGCWC 1 cut(s) 314
AlwI GGATC 2 cut(s) 85, 901
AlwNI CAGNNNCTG 1 cut(s) 710
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 7 cut(s) 137, 237, 333, 363, 399, 582, 764
ApeKI GCWGC 8 cut(s) 557, 560, 563, 619, 678, 704, 839, 842
Asp718I GGTACC 1 cut(s) 456
AspLEI GCGC 2 cut(s) 89, 967
AspS9I GGNCC 4 cut(s) 238, 364, 400, 578
AsuC2I CCSGG 2 cut(s) 64, 576
AsuHPI GGTGA 4 cut(s) 26, 173, 207, 439
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 1 cut(s) 578
BaeGI GKGCMC 1 cut(s) 526
BalI TGGCCA 1 cut(s) 766
BanI GGYRCC 2 cut(s) 456, 521
BanII GRGCYC 3 cut(s) 314, 1045, 1223
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 8 cut(s) 544, 547, 575, 631, 665, 716, 826, 854
BccI CCATC 1 cut(s) 983
BceAI ACGGC 2 cut(s) 320, 1177
BcnI CCSGG 2 cut(s) 64, 576
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 4 cut(s) 561, 693, 840, 1122
BfrI CTTAAG 1 cut(s) 851
Bme1390I CCNGG 2 cut(s) 64, 576
Bme18I GGWCC 1 cut(s) 578
BmeT110I CYCGRG 1 cut(s) 240
BmgBI CACGTC 1 cut(s) 24
BmgT120I GGNCC 4 cut(s) 238, 364, 400, 578
BmiI GGNNCC 3 cut(s) 458, 523, 1044
BmrFI CCNGG 2 cut(s) 64, 576
BmsI GCATC 4 cut(s) 254, 729, 815, 826
BpiI GAAGAC 1 cut(s) 485
BplI GAGNNNNNCTC 4 cut(s) 234, 266, 248, 280
BpmI CTGGAG 1 cut(s) 768
BpuMI CCSGG 2 cut(s) 64, 576
BsaAI YACGTR 1 cut(s) 424
BsaJI CCNNGG 2 cut(s) 535, 1290
BsaXI ACNNNNNCTCC 4 cut(s) 29, 59, 135, 165
Bsc4I CCNNNNNNNGG 4 cut(s) 466, 504, 643, 853
Bse118I RCCGGY 3 cut(s) 217, 580, 800
Bse1I ACTGG 1 cut(s) 690
Bse3DI GCAATG 5 cut(s) 126, 235, 1042, 1055, 1094
BseDI CCNNGG 2 cut(s) 535, 1290
BseGI GGATG 1 cut(s) 1300
BseLI CCNNNNNNNGG 4 cut(s) 466, 504, 643, 853
BseMI GCAATG 5 cut(s) 126, 235, 1042, 1055, 1094
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 1 cut(s) 690
BseRI GAGGAG 6 cut(s) 100, 103, 143, 190, 262, 265
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 8 cut(s) 544, 547, 575, 631, 665, 716, 826, 854
BseYI CCCAGC 1 cut(s) 1281
BsgI GTGCAG 1 cut(s) 55
Bsh1236I CGCG 3 cut(s) 89, 300, 348
BshFI GGCC 7 cut(s) 139, 239, 335, 365, 401, 584, 766
BshNI GGYRCC 2 cut(s) 456, 521
BsiHKAI GWGCWC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 5 cut(s) 64, 218, 576, 581, 801
BsiWI CGTACG 1 cut(s) 451
BslFI GGGAC 2 cut(s) 195, 356
BslI CCNNNNNNNGG 4 cut(s) 466, 504, 643, 853
BsmFI GGGAC 2 cut(s) 195, 356
BsnI GGCC 7 cut(s) 139, 239, 335, 365, 401, 584, 766
BsoBI CYCGRG 1 cut(s) 240
Bsp1286I GDGCHC 4 cut(s) 314, 526, 1045, 1223
Bsp1407I TGTACA 1 cut(s) 1269
Bsp143I GATC 2 cut(s) 90, 893
BspANI GGCC 7 cut(s) 139, 239, 335, 365, 401, 584, 766
BspCNI CTCAG 1 cut(s) 615
BspFNI CGCG 3 cut(s) 89, 300, 348
BspLI GGNNCC 3 cut(s) 458, 523, 1044
BspMAI CTGCAG 3 cut(s) 565, 844, 1126
BspPI GGATC 2 cut(s) 85, 901
BspT107I GGYRCC 2 cut(s) 456, 521
BspTI CTTAAG 1 cut(s) 851
BsrBI CCGCTC 1 cut(s) 127
BsrDI GCAATG 5 cut(s) 126, 235, 1042, 1055, 1094
BsrFI RCCGGY 3 cut(s) 217, 580, 800
BsrGI TGTACA 1 cut(s) 1269
BsrI ACTGG 1 cut(s) 690
BssAI RCCGGY 3 cut(s) 217, 580, 800
BssECI CCNNGG 2 cut(s) 535, 1290
BssMI GATC 2 cut(s) 90, 893
BssT1I CCWWGG 2 cut(s) 535, 1290
Bst4CI ACNGT 4 cut(s) 76, 456, 461, 507
Bst6I CTCTTC 1 cut(s) 1238
BstAFI CTTAAG 1 cut(s) 851
BstAPI GCANNNNNTGC 1 cut(s) 1130
BstAUI TGTACA 1 cut(s) 1269
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 3 cut(s) 363, 768, 1287
BstDEI CTNAG 1 cut(s) 623
BstF5I GGATG 1 cut(s) 1300
BstFNI CGCG 3 cut(s) 89, 300, 348
BstHHI GCGC 2 cut(s) 89, 967
BstKTI GATC 2 cut(s) 93, 896
BstMBI GATC 2 cut(s) 90, 893
BstMWI GCNNNNNNNGC 8 cut(s) 204, 256, 521, 530, 563, 788, 797, 1130
BstSCI CCNGG 2 cut(s) 62, 574
BstSFI CTRYAG 4 cut(s) 561, 693, 840, 1122
BstSLI GKGCMC 1 cut(s) 526
BstUI CGCG 3 cut(s) 89, 300, 348
BstV1I GCAGC 8 cut(s) 544, 547, 575, 631, 665, 716, 826, 854
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 1 cut(s) 893
BstXI CCANNNNNNTGG 1 cut(s) 331
BstYI RGATCY 1 cut(s) 893
BsuRI GGCC 7 cut(s) 139, 239, 335, 365, 401, 584, 766
BtgZI GCGATG 1 cut(s) 231
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 1 cut(s) 1300
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 2 cut(s) 67, 1161
Cac8I GCNNGC 3 cut(s) 363, 768, 1287
CaiI CAGNNNCTG 1 cut(s) 710
CfoI GCGC 2 cut(s) 89, 967
Cfr10I RCCGGY 3 cut(s) 217, 580, 800
Cfr13I GGNCC 4 cut(s) 238, 364, 400, 578
CpoI CGGWCCG 1 cut(s) 578
CseI GACGC 1 cut(s) 289
Csp6I GTAC 3 cut(s) 452, 457, 1270
CspI CGGWCCG 1 cut(s) 578
CviAII CATG 2 cut(s) 376, 568
CviQI GTAC 3 cut(s) 452, 457, 1270
DdeI CTNAG 1 cut(s) 623
DpnI GATC 2 cut(s) 92, 895
DpnII GATC 2 cut(s) 90, 893
DrdI GACNNNNNNGTC 2 cut(s) 22, 875
DseDI GACNNNNNNGTC 2 cut(s) 22, 875
EaeI YGGCCR 3 cut(s) 333, 582, 764
Eam1104I CTCTTC 1 cut(s) 1238
EarI CTCTTC 1 cut(s) 1238
EciI GGCGGA 2 cut(s) 37, 146
Ecl136II GAGCTC 1 cut(s) 312
Eco130I CCWWGG 2 cut(s) 535, 1290
Eco147I AGGCCT 1 cut(s) 139
Eco24I GRGCYC 3 cut(s) 314, 1045, 1223
Eco47I GGWCC 1 cut(s) 578
Eco53kI GAGCTC 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 312
EcoT14I CCWWGG 2 cut(s) 535, 1290
EcoT38I GRGCYC 3 cut(s) 314, 1045, 1223
ErhI CCWWGG 2 cut(s) 535, 1290
FaeI CATG 2 cut(s) 379, 571
FalI AAGNNNNNCTT 2 cut(s) 373, 405
FaqI GGGAC 2 cut(s) 195, 356
FatI CATG 2 cut(s) 375, 567
FauI CCCGC 1 cut(s) 212
FriOI GRGCYC 3 cut(s) 314, 1045, 1223
FspBI CTAG 1 cut(s) 527
FspI TGCGCA 1 cut(s) 966
GlaI GCGC 2 cut(s) 88, 966
GsaI CCCAGC 1 cut(s) 1285
GsuI CTGGAG 1 cut(s) 768
HaeIII GGCC 7 cut(s) 139, 239, 335, 365, 401, 584, 766
HapII CCGG 5 cut(s) 64, 218, 576, 581, 801
HgaI GACGC 1 cut(s) 289
HhaI GCGC 2 cut(s) 89, 967
Hin1II CATG 2 cut(s) 379, 571
Hin6I GCGC 2 cut(s) 87, 965
HinP1I GCGC 2 cut(s) 87, 965
HinfI GANTC 4 cut(s) 144, 598, 728, 755
HpaII CCGG 5 cut(s) 64, 218, 576, 581, 801
HphI GGTGA 4 cut(s) 26, 173, 207, 439
Hpy188I TCNGA 6 cut(s) 193, 603, 669, 727, 738, 1008
Hpy188III TCNNGA 4 cut(s) 549, 747, 897, 1144
Hpy99I CGWCG 5 cut(s) 28, 31, 305, 341, 482
HpyAV CCTTC 7 cut(s) 106, 111, 424, 647, 984, 1193, 1196
HpyCH4III ACNGT 4 cut(s) 76, 456, 461, 507
HpyCH4IV ACGT 4 cut(s) 23, 423, 605, 1089
HpyCH4V TGCA 7 cut(s) 72, 563, 681, 806, 842, 1035, 1124
HpyF10VI GCNNNNNNNGC 8 cut(s) 204, 256, 521, 530, 563, 788, 797, 1130
HpyF3I CTNAG 1 cut(s) 623
HpySE526I ACGT 4 cut(s) 23, 423, 605, 1089
Hsp92II CATG 2 cut(s) 379, 571
HspAI GCGC 2 cut(s) 87, 965
KpnI GGTACC 1 cut(s) 460
Kzo9I GATC 2 cut(s) 90, 893
LmnI GCTCC 5 cut(s) 203, 255, 317, 1016, 1048
Lsp1109I GCAGC 8 cut(s) 544, 547, 575, 631, 665, 716, 826, 854
LweI GCATC 4 cut(s) 254, 729, 815, 826
MaeI CTAG 1 cut(s) 527
MaeII ACGT 4 cut(s) 23, 423, 605, 1089
MaeIII GTNAC 4 cut(s) 472, 501, 861, 1047
MalI GATC 2 cut(s) 92, 895
MbiI CCGCTC 1 cut(s) 127
MboI GATC 2 cut(s) 90, 893
MboII GAAGA 5 cut(s) 34, 385, 485, 911, 1225
MflI RGATCY 1 cut(s) 893
MhlI GDGCHC 4 cut(s) 314, 526, 1045, 1223
MlsI TGGCCA 1 cut(s) 766
MluCI AATT 3 cut(s) 404, 883, 1167
MluI ACGCGT 1 cut(s) 298
MluNI TGGCCA 1 cut(s) 766
MlyI GAGTC 3 cut(s) 138, 607, 764
Mox20I TGGCCA 1 cut(s) 766
MscI TGGCCA 1 cut(s) 766
MseI TTAA 3 cut(s) 663, 852, 984
MslI CAYNNNNRTG 1 cut(s) 1145
Msp20I TGGCCA 1 cut(s) 766
MspA1I CMGCKG 2 cut(s) 622, 707
MspCI CTTAAG 1 cut(s) 851
MspI CCGG 5 cut(s) 64, 218, 576, 581, 801
MspR9I CCNGG 2 cut(s) 64, 576
MvnI CGCG 3 cut(s) 89, 300, 348
MwoI GCNNNNNNNGC 8 cut(s) 204, 256, 521, 530, 563, 788, 797, 1130
NciI CCSGG 2 cut(s) 64, 576
NdeII GATC 2 cut(s) 90, 893
NlaIII CATG 2 cut(s) 379, 571
NlaIV GGNNCC 3 cut(s) 458, 523, 1044
NmuCI GTSAC 2 cut(s) 501, 861
NsbI TGCGCA 1 cut(s) 966
PceI AGGCCT 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PfeI GAWTC 1 cut(s) 728
Pfl23II CGTACG 1 cut(s) 451
PfoI TCCNGGA 1 cut(s) 574
PleI GAGTC 3 cut(s) 138, 606, 763
PpsI GAGTC 3 cut(s) 138, 606, 763
Ppu21I YACGTR 1 cut(s) 424
Psp124BI GAGCTC 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 1281
PspLI CGTACG 1 cut(s) 451
PspN4I GGNNCC 3 cut(s) 458, 523, 1044
PspPI GGNCC 4 cut(s) 238, 364, 400, 578
PstI CTGCAG 3 cut(s) 565, 844, 1126
PstNI CAGNNNCTG 1 cut(s) 710
PsuI RGATCY 1 cut(s) 893
PvuII CAGCTG 1 cut(s) 622
RsaI GTAC 3 cut(s) 453, 458, 1271
RsaNI GTAC 3 cut(s) 452, 457, 1270
RseI CAYNNNNRTG 1 cut(s) 1145
Rsr2I CGGWCCG 1 cut(s) 578
RsrII CGGWCCG 1 cut(s) 578
SacI GAGCTC 1 cut(s) 314
SaqAI TTAA 3 cut(s) 663, 852, 984
Sau3AI GATC 2 cut(s) 90, 893
Sau96I GGNCC 4 cut(s) 238, 364, 400, 578
SchI GAGTC 3 cut(s) 138, 607, 764
ScrFI CCNGG 2 cut(s) 64, 576
SduI GDGCHC 4 cut(s) 314, 526, 1045, 1223
SfaNI GCATC 4 cut(s) 254, 729, 815, 826
SfcI CTRYAG 4 cut(s) 561, 693, 840, 1122
SinI GGWCC 1 cut(s) 578
SmiMI CAYNNNNRTG 1 cut(s) 1145
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
Sse9I AATT 3 cut(s) 404, 883, 1167
SseBI AGGCCT 1 cut(s) 139
SspI AATATT 1 cut(s) 1012
SspMI CTAG 1 cut(s) 527
SstI GAGCTC 1 cut(s) 314
StuI AGGCCT 1 cut(s) 139
StyD4I CCNGG 2 cut(s) 62, 574
StyI CCWWGG 2 cut(s) 535, 1290
TaaI ACNGT 4 cut(s) 76, 456, 461, 507
TaiI ACGT 4 cut(s) 26, 426, 608, 1092
TaqI TCGA 2 cut(s) 142, 440
TasI AATT 3 cut(s) 404, 883, 1167
TatI WGTACW 1 cut(s) 1269
TauI GCSGC 4 cut(s) 127, 262, 515, 710
TfiI GAWTC 1 cut(s) 728
Tru1I TTAA 3 cut(s) 663, 852, 984
Tru9I TTAA 3 cut(s) 663, 852, 984
TscAI CASTG 2 cut(s) 74, 1161
TseFI GTSAC 2 cut(s) 501, 861
TseI GCWGC 8 cut(s) 557, 560, 563, 619, 678, 704, 839, 842
Tsp45I GTSAC 2 cut(s) 501, 861
TspDTI ATGAA 2 cut(s) 392, 966
TspGWI ACGGA 1 cut(s) 1035
TspRI CASTG 2 cut(s) 74, 1161
Vha464I CTTAAG 1 cut(s) 851
VpaK11BI GGWCC 1 cut(s) 578
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.