pycom10g05340

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002209
Physical Location & Seq
Forward (+)
5688940 .. 5691704
2765 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 939 bp
ATGACGGAGAAAAGCGGCGACGGCGAGGGCAGCGGTGGGGGTGATGGTGGTCCAGCAGAGGAGGAGAAGAATAATAATAATGCTGATGGTGGTGTTCCACCAGAGGAAGAGAAGGAGAAAGAGAAGAATGGAGATCCAGAACTGCTGATCTGCTTCCTTCAACCTGCTCCCGCCGACTCCGATCCCGACTACATTGGCATCCGTCGCCTTCTCCTCTCTCGCAAGCCCCAGTCCCCATTTCATCGACGCCGAGATTGGAGATGCAATGGAAAAGGGTACGTCGCGTTTCGAAATTACATCCGGCGGCCGAGGAATTGGGAGAGGATGCAGACTCCGAGCCTCCAGACCACTCCCGGAAACAGTGGGCGATGGATTCTGCCTACAAGTCCACTATCCCTTTTGTACGACGTCGAAAGCTGGAGTCCTGGCAGGGACGTCCAGAGTGGCAGTCAACCTTCACCTCGCAGAAGTTTTGGCTCCAGTATAAGTGATAGTGACCGCCCACATCCTCGGCGGGCAGAACCTGCATACTCGTTTGTAGGAATGCATTGCATCTTTGATCAGTGCAAAGCCTCTGTTACGGTTTTGAAGTTTGGGTACATGAGTTCTGATCTGCTTGCATATGGAGCATCAGATGGAACCTTGACAGTATGCACTGTTTCTGATCCACCTTCAATCCTCAAGCATCTGCATGGTCACTCCAAAGATGTTACAGACTTTGATTTTTCGTCGAACAATCAATACATTGCATCCTCATCAATGGATAAAACTGTACGAGTGTGGGAGATTTCAAAAGGCCTTTGTATTCGAGTAATATATGGAGTGTCTCCGCAACTGTGTATCCATTTTCACCCTGTAAATAATAACTTTCTTTCAGCTGGCAATGCAGACAAAGAAGTTACGGTAATGACTGCATACCTTATGTTCATTCACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

313

Amino Acids

34.67

Weight (kDa)

6.75

Isoelectric Point (pI)

61.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 183 - 302 2.7e-16 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 187 - 266 6.3e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 188 - 302 2.1e-16 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 189 - 275 6.6e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 191 - 295 6.7e-12 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 193 - 302 1e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 201 - 297 1e-11 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 201 - 264 1.9e-06 WDR55
EIF3I PF24805 202 - 293 5.2e-06 EIF3I
Beta-prop_THOC3 PF25174 203 - 303 1.4e-12 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 222 - 298 1.1e-06 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 226 - 274 4e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 227 - 261 7.6e-11 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 411, 438
Acc36I ACCTGC 2 cut(s) 172, 532
AccII CGCG 1 cut(s) 284
AciI CCGC 7 cut(s) 15, 33, 171, 304, 499, 514, 830
AclWI GGATC 3 cut(s) 128, 176, 659
AcoI YGGCCR 1 cut(s) 305
AcyI GRCGYC 3 cut(s) 247, 408, 435
AfaI GTAC 4 cut(s) 278, 404, 599, 774
AgsI TTSAA 4 cut(s) 161, 589, 675, 792
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 2 cut(s) 417, 878
AluI AGCT 2 cut(s) 417, 878
Alw26I GTCTC 1 cut(s) 831
AlwI GGATC 3 cut(s) 128, 176, 659
AlwNI CAGNNNCTG 1 cut(s) 524
AoxI GGCC 2 cut(s) 305, 796
ApeKI GCWGC 1 cut(s) 30
AspS9I GGNCC 1 cut(s) 50
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 4 cut(s) 53, 450, 842, 923
AsuII TTCGAA 1 cut(s) 289
AvaII GGWCC 1 cut(s) 50
BaeI ACNNNNGTAYC 2 cut(s) 589, 622
BarI GAAGNNNNNNTAC 2 cut(s) 581, 613
BbvI GCAGC 1 cut(s) 42
BccI CCATC 4 cut(s) 38, 80, 363, 629
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 426
BciVI GTATCC 1 cut(s) 851
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 831
BfuAI ACCTGC 2 cut(s) 172, 532
BfuI GTATCC 1 cut(s) 851
BisI GCNGC 3 cut(s) 16, 31, 305
BlsI GCNGC 3 cut(s) 17, 32, 306
Bme1390I CCNGG 2 cut(s) 354, 426
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 2 cut(s) 478, 640
BmrFI CCNGG 2 cut(s) 354, 426
BmrI ACTGGG 1 cut(s) 223
BmsI GCATC 7 cut(s) 207, 251, 315, 561, 638, 694, 758
BmuI ACTGGG 1 cut(s) 223
BpmI CTGGAG 3 cut(s) 326, 439, 463
Bpu14I TTCGAA 1 cut(s) 289
BpuEI CTTGAG 1 cut(s) 665
BpuMI CCSGG 1 cut(s) 354
BsaHI GRCGYC 3 cut(s) 247, 408, 435
BsaJI CCNNGG 2 cut(s) 308, 509
Bse1I ACTGG 2 cut(s) 229, 480
Bse3DI GCAATG 4 cut(s) 271, 547, 744, 889
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 2 cut(s) 308, 509
BseGI GGATG 5 cut(s) 198, 297, 330, 505, 749
BseMI GCAATG 4 cut(s) 271, 547, 744, 889
BseNI ACTGG 2 cut(s) 229, 480
BseRI GAGGAG 3 cut(s) 74, 77, 203
BseX3I CGGCCG 1 cut(s) 305
BseXI GCAGC 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 284
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 2 cut(s) 307, 798
BsiEI CGRYCG 1 cut(s) 308
BsiSI CCGG 2 cut(s) 301, 354
BslFI GGGAC 2 cut(s) 217, 446
BsmAI GTCTC 1 cut(s) 831
BsmFI GGGAC 2 cut(s) 217, 446
BsmI GAATGC 1 cut(s) 549
BsnI GGCC 2 cut(s) 307, 798
Bsp119I TTCGAA 1 cut(s) 289
Bsp143I GATC 6 cut(s) 133, 147, 181, 559, 610, 664
BspACI CCGC 7 cut(s) 15, 33, 171, 304, 499, 514, 830
BspANI GGCC 2 cut(s) 307, 798
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 2 cut(s) 478, 640
BspMI ACCTGC 2 cut(s) 172, 532
BspPI GGATC 3 cut(s) 128, 176, 659
BspT104I TTCGAA 1 cut(s) 289
BsrDI GCAATG 4 cut(s) 271, 547, 744, 889
BsrI ACTGG 2 cut(s) 229, 480
BssECI CCNNGG 2 cut(s) 308, 509
BssMI GATC 6 cut(s) 133, 147, 181, 559, 610, 664
BssNI GRCGYC 3 cut(s) 247, 408, 435
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 8 cut(s) 362, 583, 649, 658, 772, 837, 904, 935
Bst6I CTCTTC 1 cut(s) 102
BstACI GRCGYC 3 cut(s) 247, 408, 435
BstAPI GCANNNNNTGC 1 cut(s) 524
BstBI TTCGAA 1 cut(s) 289
BstC8I GCNNGC 4 cut(s) 224, 516, 618, 880
BstF5I GGATG 5 cut(s) 198, 297, 330, 505, 749
BstFNI CGCG 1 cut(s) 284
BstKTI GATC 6 cut(s) 136, 150, 184, 562, 613, 667
BstMAI GTCTC 1 cut(s) 831
BstMBI GATC 6 cut(s) 133, 147, 181, 559, 610, 664
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 6 cut(s) 21, 30, 204, 524, 626, 884
BstNI CCWGG 1 cut(s) 426
BstSCI CCNGG 2 cut(s) 352, 424
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BstZI CGGCCG 1 cut(s) 305
BsuI GTATCC 1 cut(s) 851
BsuRI GGCC 2 cut(s) 307, 798
BtgZI GCGATG 1 cut(s) 382
BtsCI GGATG 5 cut(s) 198, 297, 330, 505, 749
BtsIMutI CAGTG 3 cut(s) 367, 569, 654
BveI ACCTGC 2 cut(s) 172, 532
Cac8I GCNNGC 4 cut(s) 224, 516, 618, 880
CaiI CAGNNNCTG 1 cut(s) 524
Cfr13I GGNCC 1 cut(s) 50
CseI GACGC 1 cut(s) 255
Csp6I GTAC 4 cut(s) 277, 403, 598, 773
CviAII CATG 2 cut(s) 601, 692
CviJI RGCY 8 cut(s) 226, 307, 339, 417, 477, 572, 798, 878
CviKI_1 RGCY 8 cut(s) 226, 307, 339, 417, 477, 572, 798, 878
CviQI GTAC 4 cut(s) 277, 403, 598, 773
DpnI GATC 6 cut(s) 135, 149, 183, 561, 612, 666
DpnII GATC 6 cut(s) 133, 147, 181, 559, 610, 664
EaeI YGGCCR 1 cut(s) 305
EagI CGGCCG 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
EclXI CGGCCG 1 cut(s) 305
Eco147I AGGCCT 1 cut(s) 798
Eco47I GGWCC 1 cut(s) 50
Eco52I CGGCCG 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 549
FaeI CATG 2 cut(s) 604, 695
FaqI GGGAC 2 cut(s) 217, 446
FatI CATG 2 cut(s) 600, 691
FauI CCCGC 2 cut(s) 178, 507
FauNDI CATATG 1 cut(s) 622
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 3 cut(s) 16, 31, 305
FokI GGATG 5 cut(s) 185, 284, 337, 492, 736
Fsp4HI GCNGC 3 cut(s) 16, 31, 305
GluI GCNGC 3 cut(s) 16, 31, 305
GsuI CTGGAG 3 cut(s) 326, 439, 463
HaeIII GGCC 2 cut(s) 307, 798
HapII CCGG 2 cut(s) 301, 354
HgaI GACGC 1 cut(s) 255
Hin1I GRCGYC 3 cut(s) 247, 408, 435
Hin1II CATG 2 cut(s) 604, 695
HincII GTYRAC 1 cut(s) 452
HindII GTYRAC 1 cut(s) 452
HinfI GANTC 4 cut(s) 176, 331, 373, 421
HpaII CCGG 2 cut(s) 301, 354
HphI GGTGA 4 cut(s) 53, 450, 842, 923
Hpy166II GTNNAC 2 cut(s) 389, 452
Hpy188I TCNGA 5 cut(s) 181, 336, 610, 634, 664
Hpy188III TCNNGA 4 cut(s) 137, 185, 343, 439
Hpy8I GTNNAC 2 cut(s) 389, 452
Hpy99I CGWCG 7 cut(s) 23, 207, 249, 284, 410, 413, 733
HpyAV CCTTC 5 cut(s) 106, 167, 218, 465, 681
HpyCH4III ACNGT 8 cut(s) 362, 583, 649, 658, 772, 837, 904, 935
HpyCH4IV ACGT 3 cut(s) 279, 408, 435
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 30, 204, 524, 626, 884
HpySE526I ACGT 3 cut(s) 279, 408, 435
Hsp92I GRCGYC 3 cut(s) 247, 408, 435
Hsp92II CATG 2 cut(s) 604, 695
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 6 cut(s) 133, 147, 181, 559, 610, 664
LmnI GCTCC 3 cut(s) 172, 482, 626
Lsp1109I GCAGC 1 cut(s) 42
LweI GCATC 7 cut(s) 207, 251, 315, 561, 638, 694, 758
MaeII ACGT 3 cut(s) 279, 408, 435
MaeIII GTNAC 5 cut(s) 494, 577, 695, 709, 898
MalI GATC 6 cut(s) 135, 149, 183, 561, 612, 666
MboI GATC 6 cut(s) 133, 147, 181, 559, 610, 664
MboII GAAGA 3 cut(s) 79, 119, 136
MflI RGATCY 1 cut(s) 133
MluCI AATT 2 cut(s) 292, 313
MlyI GAGTC 3 cut(s) 170, 325, 430
Mph1103I ATGCAT 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 690
MspA1I CMGCKG 2 cut(s) 33, 878
MspI CCGG 2 cut(s) 301, 354
MspR9I CCNGG 2 cut(s) 354, 426
Mva1269I GAATGC 1 cut(s) 549
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 6 cut(s) 21, 30, 204, 524, 626, 884
NciI CCSGG 1 cut(s) 354
NdeI CATATG 1 cut(s) 622
NdeII GATC 6 cut(s) 133, 147, 181, 559, 610, 664
NlaIII CATG 2 cut(s) 604, 695
NlaIV GGNNCC 2 cut(s) 478, 640
NmeAIII GCCGAG 3 cut(s) 275, 333, 490
NmuCI GTSAC 2 cut(s) 494, 695
NsiI ATGCAT 1 cut(s) 549
NspV TTCGAA 1 cut(s) 289
PceI AGGCCT 1 cut(s) 798
PctI GAATGC 1 cut(s) 549
PfeI GAWTC 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 352
PkrI GCNGC 3 cut(s) 17, 32, 306
PleI GAGTC 3 cut(s) 170, 325, 429
PpsI GAGTC 3 cut(s) 170, 325, 429
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 2 cut(s) 478, 640
PspPI GGNCC 1 cut(s) 50
PsrI GAACNNNNNNTAC 2 cut(s) 725, 757
PstNI CAGNNNCTG 1 cut(s) 524
PsuI RGATCY 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 4 cut(s) 278, 404, 599, 774
RsaNI GTAC 4 cut(s) 277, 403, 598, 773
RseI CAYNNNNRTG 1 cut(s) 690
SatI GCNGC 3 cut(s) 16, 31, 305
Sau3AI GATC 6 cut(s) 133, 147, 181, 559, 610, 664
Sau96I GGNCC 1 cut(s) 50
SchI GAGTC 3 cut(s) 170, 325, 430
ScrFI CCNGG 2 cut(s) 354, 426
SfaNI GCATC 7 cut(s) 207, 251, 315, 561, 638, 694, 758
SfuI TTCGAA 1 cut(s) 289
SinI GGWCC 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 690
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 2 cut(s) 292, 313
SseBI AGGCCT 1 cut(s) 798
SsiI CCGC 7 cut(s) 15, 33, 171, 304, 499, 514, 830
StuI AGGCCT 1 cut(s) 798
StyD4I CCNGG 2 cut(s) 352, 424
TaaI ACNGT 8 cut(s) 362, 583, 649, 658, 772, 837, 904, 935
TaiI ACGT 3 cut(s) 282, 411, 438
TaqI TCGA 5 cut(s) 244, 289, 411, 731, 808
TasI AATT 2 cut(s) 292, 313
TauI GCSGC 2 cut(s) 18, 307
TfiI GAWTC 1 cut(s) 373
TscAI CASTG 3 cut(s) 367, 569, 661
TseFI GTSAC 2 cut(s) 494, 695
TseI GCWGC 1 cut(s) 30
Tsp45I GTSAC 2 cut(s) 494, 695
TspDTI ATGAA 2 cut(s) 230, 916
TspGWI ACGGA 2 cut(s) 20, 191
TspRI CASTG 3 cut(s) 367, 569, 661
VpaK11BI GGWCC 1 cut(s) 50
ZraI GACGTC 2 cut(s) 409, 436
Zsp2I ATGCAT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.