pycom10g16830

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004190
Physical Location & Seq
Reverse (-)
20054321 .. 20056143
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGGATATGGGTTCGATCATATATTCTCTAGGCAGGGTCTTCTTGTTATTCTTCTTGTTGGTATGGGAAACAGAAGGTCGTGATCAAGCCAAGTATTTCGACGTGAGGAAATATGGTGCTGTTGATGATGGAAAAACTGATAACAGCCAGGCATTTCTGGATGCATGGAAAGAAGCATGTCAATGGAAAGGAACGGCAAGGGTTGTGGTTCCCGGAGGAACATTCAAGTTGTATCCAGTGATATTCTCAGGTCCATGCAATGGTCCGATTGCCTTTCTGATTAAGGGTACTTTGAGTGCTACGACTAATCCATCAACATTTAGTGCTCACAGTTGGATAAACTTCCGGTACATCGATCAATTGACAGTGACCGGAGGCGGTACCTTGGACGGCCAAGGAGCCTCGGCTTGGCATCTTAACAACTGCAAAACCAATCCCCAATGCCAGGCTCTTCCTATTTCAATGAGTTTTGACTTCATCACAAACGCAAGAGTCGAATACATAAGATCATACGACAGCAAAAATGCACATTTCAAAATTTTCGGATGCCACAATATGGGCTTTAGGAAAATCAAAATCTCAGCTCCGGCTGACAGTCCGAACACCGATGGCATTAAGATTGGAAGCTCATACCGAATCAGAATCGCACGCTCTACGATCGCCACGGGTGATGACTGTGTCGCGATGGTGCACGGAAGTAGGAAAATCCACATTTCTAAAGTTGTGTGCGGTCCCGGGCATGGAATCAGCATCGGAAGCCTAGGAGGGAATGACAAAGAAGACGATGTGGTCGGAGTGACTGTGAAAAACTGCACCTTTTTGGGGACGGATAATGGTGTGAGGATCAAAACTTGGGCTTCTCCTTATACCAGTAAGGCTTCAAATTTTACATTCCAAGACATTTTCATGGATGACGTTCTGAATCCTATTACCATCGATCAAGAATATTGCCCCAACCCTCCTTGCAATCAACAGACTTCTTCGAATGTGCAAATCAGCGACGTTACGTACAAAAATATATGGGGAAGTTCGTCGTCGGAAGTAGCCGTCAGTTTCCGATGCAGCAAAACCTGCCCATGCAAGAACGTACTGCTGGACAGCATCAAAATGTCCCATTCTAATCCCAGAGGATGCTTGCGTTCCTTCTGCAAGTATGCCAAAGGCTCCTCTTATGGCTCACAAAGCCCTCCATCTTGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

401

Amino Acids

43.73

Weight (kDa)

8.77

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 60 - 374 1.7e-94 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 788
Acc36I ACCTGC 1 cut(s) 1079
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 1 cut(s) 380
AccB7I CCANNNNNTGG 2 cut(s) 260, 556
AccII CGCG 1 cut(s) 683
AciI CCGC 2 cut(s) 378, 729
AclWI GGATC 1 cut(s) 851
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 2 cut(s) 537, 883
AfaI GTAC 5 cut(s) 289, 350, 382, 1010, 1089
AfiI CCNNNNNNNGG 6 cut(s) 260, 408, 445, 556, 740, 822
AgsI TTSAA 4 cut(s) 226, 462, 535, 882
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 2 cut(s) 147, 444
AluBI AGCT 2 cut(s) 584, 627
AluI AGCT 2 cut(s) 584, 627
Alw21I GWGCWC 2 cut(s) 328, 693
Alw44I GTGCAC 1 cut(s) 689
AlwI GGATC 1 cut(s) 851
Ama87I CYCGRG 1 cut(s) 734
AoxI GGCC 1 cut(s) 391
ApaLI GTGCAC 1 cut(s) 689
ApeKI GCWGC 1 cut(s) 1062
ApoI RAATTY 2 cut(s) 537, 883
Asp718I GGTACC 1 cut(s) 380
AspA2I CCTAGG 1 cut(s) 760
AspS9I GGNCC 3 cut(s) 251, 263, 731
AsuC2I CCSGG 3 cut(s) 213, 735, 736
AsuHPI GGTGA 1 cut(s) 680
AsuII TTCGAA 1 cut(s) 983
AvaI CYCGRG 1 cut(s) 734
AvaII GGWCC 3 cut(s) 251, 263, 731
AvrII CCTAGG 1 cut(s) 760
BaeGI GKGCMC 1 cut(s) 693
BanI GGYRCC 1 cut(s) 380
BbsI GAAGAC 2 cut(s) 31, 786
Bbv12I GWGCWC 2 cut(s) 328, 693
BbvI GCAGC 1 cut(s) 1074
BccI CCATC 6 cut(s) 122, 319, 602, 679, 941, 1198
BceAI ACGGC 3 cut(s) 210, 406, 1031
BciT130I CCWGG 2 cut(s) 149, 446
BciVI GTATCC 1 cut(s) 243
BclI TGATCA 1 cut(s) 82
BcnI CCSGG 3 cut(s) 213, 735, 736
BfaI CTAG 2 cut(s) 29, 761
BfuAI ACCTGC 1 cut(s) 1079
BfuI GTATCC 1 cut(s) 243
BisI GCNGC 1 cut(s) 1063
BlnI CCTAGG 1 cut(s) 760
BlsI GCNGC 1 cut(s) 1064
Bme1390I CCNGG 5 cut(s) 149, 213, 446, 735, 736
Bme18I GGWCC 3 cut(s) 251, 263, 731
BmeT110I CYCGRG 1 cut(s) 734
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 251, 263, 731
BmiI GGNNCC 5 cut(s) 210, 382, 400, 733, 1165
BmrFI CCNGG 5 cut(s) 149, 213, 446, 735, 736
BmsI GCATC 7 cut(s) 151, 421, 536, 759, 1049, 1110, 1121
BpiI GAAGAC 2 cut(s) 31, 786
Bpu14I TTCGAA 1 cut(s) 983
BpuMI CCSGG 3 cut(s) 213, 735, 736
Bsa29I ATCGAT 2 cut(s) 354, 936
BsaAI YACGTR 1 cut(s) 1008
BsaJI CCNNGG 6 cut(s) 384, 394, 402, 663, 734, 760
BsaWI WCCGGW 2 cut(s) 345, 371
BsaXI ACNNNNNCTCC 2 cut(s) 786, 816
Bsc4I CCNNNNNNNGG 6 cut(s) 260, 408, 445, 556, 740, 822
Bse1I ACTGG 2 cut(s) 236, 870
Bse3DI GCAATG 1 cut(s) 265
BseBI CCWGG 2 cut(s) 149, 446
BseCI ATCGAT 2 cut(s) 354, 936
BseDI CCNNGG 6 cut(s) 384, 394, 402, 663, 734, 760
BseGI GGATG 4 cut(s) 166, 551, 916, 1136
BseLI CCNNNNNNNGG 6 cut(s) 260, 408, 445, 556, 740, 822
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 2 cut(s) 261, 594
BseNI ACTGG 2 cut(s) 236, 870
BseRI GAGGAG 1 cut(s) 1156
BseSI GKGCMC 1 cut(s) 693
BseXI GCAGC 1 cut(s) 1074
BsgI GTGCAG 1 cut(s) 796
Bsh1236I CGCG 1 cut(s) 683
Bsh1285I CGRYCG 1 cut(s) 660
BshFI GGCC 1 cut(s) 393
BshNI GGYRCC 1 cut(s) 380
BshVI ATCGAT 2 cut(s) 354, 936
BsiEI CGRYCG 1 cut(s) 660
BsiHKAI GWGCWC 2 cut(s) 328, 693
BsiHKCI CYCGRG 1 cut(s) 734
BsiSI CCGG 5 cut(s) 213, 346, 372, 587, 735
BslFI GGGAC 3 cut(s) 717, 838, 1096
BslI CCNNNNNNNGG 6 cut(s) 260, 408, 445, 556, 740, 822
BsmFI GGGAC 3 cut(s) 717, 838, 1096
BsnI GGCC 1 cut(s) 393
BsoBI CYCGRG 1 cut(s) 734
Bsp119I TTCGAA 1 cut(s) 983
Bsp1286I GDGCHC 2 cut(s) 328, 693
Bsp143I GATC 7 cut(s) 15, 82, 355, 506, 657, 843, 937
Bsp68I TCGCGA 1 cut(s) 683
BspACI CCGC 2 cut(s) 378, 729
BspANI GGCC 1 cut(s) 393
BspCNI CTCAG 2 cut(s) 260, 593
BspDI ATCGAT 2 cut(s) 354, 936
BspFNI CGCG 1 cut(s) 683
BspLI GGNNCC 5 cut(s) 210, 382, 400, 733, 1165
BspMI ACCTGC 1 cut(s) 1079
BspPI GGATC 1 cut(s) 851
BspQI GCTCTTC 1 cut(s) 456
BspT104I TTCGAA 1 cut(s) 983
BspT107I GGYRCC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 265
BsrI ACTGG 2 cut(s) 236, 870
BssECI CCNNGG 6 cut(s) 384, 394, 402, 663, 734, 760
BssMI GATC 7 cut(s) 15, 82, 355, 506, 657, 843, 937
BssT1I CCWWGG 3 cut(s) 384, 394, 760
Bst2UI CCWGG 2 cut(s) 149, 446
Bst4CI ACNGT 5 cut(s) 332, 367, 596, 677, 802
Bst6I CTCTTC 1 cut(s) 456
BstAPI GCANNNNNTGC 1 cut(s) 1071
BstBAI YACGTR 1 cut(s) 1008
BstBI TTCGAA 1 cut(s) 983
BstC8I GCNNGC 2 cut(s) 649, 1136
BstDEI CTNAG 2 cut(s) 247, 580
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 4 cut(s) 166, 551, 916, 1136
BstFNI CGCG 1 cut(s) 683
BstKTI GATC 7 cut(s) 18, 85, 358, 509, 660, 846, 940
BstMBI GATC 7 cut(s) 15, 82, 355, 506, 657, 843, 937
BstMCI CGRYCG 1 cut(s) 660
BstMWI GCNNNNNNNGC 3 cut(s) 756, 1071, 1182
BstNI CCWGG 2 cut(s) 149, 446
BstNSI RCATGY 1 cut(s) 180
BstSCI CCNGG 5 cut(s) 147, 211, 444, 733, 734
BstSLI GKGCMC 1 cut(s) 693
BstSNI TACGTA 1 cut(s) 1008
BstUI CGCG 1 cut(s) 683
BstV1I GCAGC 1 cut(s) 1074
BstV2I GAAGAC 2 cut(s) 31, 786
Bsu15I ATCGAT 2 cut(s) 354, 936
BsuI GTATCC 1 cut(s) 243
BsuRI GGCC 1 cut(s) 393
BsuTUI ATCGAT 2 cut(s) 354, 936
BtgI CCRYGG 1 cut(s) 663
BtgZI GCGATG 1 cut(s) 698
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 4 cut(s) 166, 551, 916, 1136
BtsIMutI CAGTG 2 cut(s) 243, 372
BtuMI TCGCGA 1 cut(s) 683
BveI ACCTGC 1 cut(s) 1079
Cac8I GCNNGC 2 cut(s) 649, 1136
Cfr13I GGNCC 3 cut(s) 251, 263, 731
Cfr9I CCCGGG 1 cut(s) 734
ClaI ATCGAT 2 cut(s) 354, 936
Csp6I GTAC 5 cut(s) 288, 349, 381, 1009, 1088
CspCI CAANNNNNGTGG 2 cut(s) 186, 221
CviAII CATG 6 cut(s) 165, 177, 255, 740, 907, 1077
CviQI GTAC 5 cut(s) 288, 349, 381, 1009, 1088
DdeI CTNAG 2 cut(s) 247, 580
DpnI GATC 7 cut(s) 17, 84, 357, 508, 659, 845, 939
DpnII GATC 7 cut(s) 15, 82, 355, 506, 657, 843, 937
DrdI GACNNNNNNGTC 1 cut(s) 788
DseDI GACNNNNNNGTC 1 cut(s) 788
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco105I TACGTA 1 cut(s) 1008
Eco130I CCWWGG 3 cut(s) 384, 394, 760
Eco47I GGWCC 3 cut(s) 251, 263, 731
Eco88I CYCGRG 1 cut(s) 734
EcoRII CCWGG 2 cut(s) 147, 444
EcoT14I CCWWGG 3 cut(s) 384, 394, 760
EcoT22I ATGCAT 1 cut(s) 166
ErhI CCWWGG 3 cut(s) 384, 394, 760
FaeI CATG 6 cut(s) 168, 180, 258, 743, 910, 1080
FaqI GGGAC 3 cut(s) 717, 838, 1096
FatI CATG 6 cut(s) 164, 176, 254, 739, 906, 1076
FbaI TGATCA 1 cut(s) 82
Fnu4HI GCNGC 1 cut(s) 1063
FokI GGATG 4 cut(s) 173, 558, 923, 1143
Fsp4HI GCNGC 1 cut(s) 1063
FspBI CTAG 2 cut(s) 29, 761
GluI GCNGC 1 cut(s) 1063
HaeIII GGCC 1 cut(s) 393
HapII CCGG 5 cut(s) 213, 346, 372, 587, 735
Hin1II CATG 6 cut(s) 168, 180, 258, 743, 910, 1080
HinfI GANTC 5 cut(s) 492, 636, 642, 744, 922
HpaII CCGG 5 cut(s) 213, 346, 372, 587, 735
HphI GGTGA 1 cut(s) 680
Hpy166II GTNNAC 1 cut(s) 691
Hpy188III TCNNGA 4 cut(s) 80, 158, 682, 941
Hpy8I GTNNAC 1 cut(s) 691
Hpy99I CGWCG 4 cut(s) 104, 1004, 1036, 1039
HpyAV CCTTC 2 cut(s) 68, 1153
HpyCH4III ACNGT 5 cut(s) 332, 367, 596, 677, 802
HpyCH4IV ACGT 5 cut(s) 102, 915, 1002, 1007, 1086
HpyF10VI GCNNNNNNNGC 3 cut(s) 756, 1071, 1182
HpyF3I CTNAG 2 cut(s) 247, 580
HpySE526I ACGT 5 cut(s) 102, 915, 1002, 1007, 1086
Hsp92II CATG 6 cut(s) 168, 180, 258, 743, 910, 1080
KpnI GGTACC 1 cut(s) 384
Ksp22I TGATCA 1 cut(s) 82
Kzo9I GATC 7 cut(s) 15, 82, 355, 506, 657, 843, 937
LguI GCTCTTC 1 cut(s) 456
LmnI GCTCC 3 cut(s) 398, 589, 1169
Lsp1109I GCAGC 1 cut(s) 1074
LweI GCATC 7 cut(s) 151, 421, 536, 759, 1049, 1110, 1121
MaeI CTAG 2 cut(s) 29, 761
MaeII ACGT 5 cut(s) 102, 915, 1002, 1007, 1086
MaeIII GTNAC 3 cut(s) 367, 796, 1003
MalI GATC 7 cut(s) 17, 84, 357, 508, 659, 845, 939
MboI GATC 7 cut(s) 15, 82, 355, 506, 657, 843, 937
MboII GAAGA 5 cut(s) 31, 43, 443, 791, 972
MfeI CAATTG 1 cut(s) 359
MhlI GDGCHC 2 cut(s) 328, 693
MluCI AATT 3 cut(s) 359, 537, 883
MlyI GAGTC 1 cut(s) 501
MmeI TCCRAC 3 cut(s) 314, 772, 1017
Mph1103I ATGCAT 1 cut(s) 166
MseI TTAA 3 cut(s) 282, 417, 615
MslI CAYNNNNRTG 3 cut(s) 181, 905, 1106
MspI CCGG 5 cut(s) 213, 346, 372, 587, 735
MspR9I CCNGG 5 cut(s) 149, 213, 446, 735, 736
MunI CAATTG 1 cut(s) 359
MvaI CCWGG 2 cut(s) 149, 446
MvnI CGCG 1 cut(s) 683
MwoI GCNNNNNNNGC 3 cut(s) 756, 1071, 1182
NciI CCSGG 3 cut(s) 213, 735, 736
NdeII GATC 7 cut(s) 15, 82, 355, 506, 657, 843, 937
NlaIII CATG 6 cut(s) 168, 180, 258, 743, 910, 1080
NlaIV GGNNCC 5 cut(s) 210, 382, 400, 733, 1165
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 2 cut(s) 367, 796
NruI TCGCGA 1 cut(s) 683
NsiI ATGCAT 1 cut(s) 166
NspI RCATGY 1 cut(s) 180
NspV TTCGAA 1 cut(s) 983
PciSI GCTCTTC 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PfeI GAWTC 4 cut(s) 636, 642, 744, 922
PflFI GACNNNGTC 1 cut(s) 677
PflMI CCANNNNNTGG 2 cut(s) 260, 556
PfoI TCCNGGA 1 cut(s) 211
PkrI GCNGC 1 cut(s) 1064
Ple19I CGATCG 1 cut(s) 660
PleI GAGTC 1 cut(s) 500
PpsI GAGTC 1 cut(s) 500
Ppu21I YACGTR 1 cut(s) 1008
Psp6I CCWGG 2 cut(s) 147, 444
PspGI CCWGG 2 cut(s) 147, 444
PspN4I GGNNCC 5 cut(s) 210, 382, 400, 733, 1165
PspPI GGNCC 3 cut(s) 251, 263, 731
PsyI GACNNNGTC 1 cut(s) 677
PvuI CGATCG 1 cut(s) 660
RruI TCGCGA 1 cut(s) 683
RsaI GTAC 5 cut(s) 289, 350, 382, 1010, 1089
RsaNI GTAC 5 cut(s) 288, 349, 381, 1009, 1088
RseI CAYNNNNRTG 3 cut(s) 181, 905, 1106
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 3 cut(s) 282, 417, 615
SatI GCNGC 1 cut(s) 1063
Sau3AI GATC 7 cut(s) 15, 82, 355, 506, 657, 843, 937
Sau96I GGNCC 3 cut(s) 251, 263, 731
SchI GAGTC 1 cut(s) 501
ScrFI CCNGG 5 cut(s) 149, 213, 446, 735, 736
SduI GDGCHC 2 cut(s) 328, 693
SfaNI GCATC 7 cut(s) 151, 421, 536, 759, 1049, 1110, 1121
SfuI TTCGAA 1 cut(s) 983
SinI GGWCC 3 cut(s) 251, 263, 731
SmaI CCCGGG 1 cut(s) 736
SmiMI CAYNNNNRTG 3 cut(s) 181, 905, 1106
SnaBI TACGTA 1 cut(s) 1008
Sse9I AATT 3 cut(s) 359, 537, 883
SsiI CCGC 2 cut(s) 378, 729
SspI AATATT 1 cut(s) 947
SspMI CTAG 2 cut(s) 29, 761
StyD4I CCNGG 5 cut(s) 147, 211, 444, 733, 734
StyI CCWWGG 3 cut(s) 384, 394, 760
TaaI ACNGT 5 cut(s) 332, 367, 596, 677, 802
TaiI ACGT 5 cut(s) 105, 918, 1005, 1010, 1089
TaqI TCGA 6 cut(s) 14, 99, 354, 495, 936, 983
TasI AATT 3 cut(s) 359, 537, 883
TfiI GAWTC 4 cut(s) 636, 642, 744, 922
Tru1I TTAA 3 cut(s) 282, 417, 615
Tru9I TTAA 3 cut(s) 282, 417, 615
TscAI CASTG 2 cut(s) 243, 372
TseFI GTSAC 2 cut(s) 367, 796
TseI GCWGC 1 cut(s) 1062
Tsp45I GTSAC 2 cut(s) 367, 796
TspDTI ATGAA 2 cut(s) 466, 895
TspGWI ACGGA 2 cut(s) 708, 842
TspMI CCCGGG 1 cut(s) 734
TspRI CASTG 2 cut(s) 243, 372
Tth111I GACNNNGTC 1 cut(s) 677
Van91I CCANNNNNTGG 2 cut(s) 260, 556
VneI GTGCAC 1 cut(s) 689
VpaK11BI GGWCC 3 cut(s) 251, 263, 731
XapI RAATTY 2 cut(s) 537, 883
XceI RCATGY 1 cut(s) 180
XmaI CCCGGG 1 cut(s) 734
XmaJI CCTAGG 1 cut(s) 760
XspI CTAG 2 cut(s) 29, 761
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.