pycom111g01910

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0001946
Physical Location & Seq
Forward (+)
1146212 .. 1147529
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGAGCATTCAGAAGCAAACTAAATGGTGGTATGCCAAGCGGTTTTTGCATCCTGACATTGTGGCACCGTACGAATACGTATTTATTTGGGATGAGGATCTTGGTCTGGAACACTTTGATGCTGAGAAATACATAAAATTGGTAAAGAAACATGGTTTGGAAATATCACAGCCAGGTTTAGATCCAAAGAGTGGGCTAACATGGCAGATGACAAGGCGGAGAGAAAACAGCGAAGTTCACATGCAGACAGAGGAGAGACCTGGTTGGTGCAGTGATCCGCATTTGCCACCATGTGCATCGTTTGTTGAAATTATGGCACCTGTGTTTTCTCGGGATGCATGGCGTTGTGTCTGGCATATGATTCAGAATGACCTGGTCCATGGCTGGGGTCTGGATTTCGCTCTTAGAAATTGTGTTGAGCCTGCACATAAGAAAATTGGAGTCGTGGACGCGCAATGGATTGTCCATCAAGGCGTTCCTTCACTCGGGAACCAAGGGCAAGCGGAGGGTGGAAGAGCACCATGGGAAGGGGTAAGGGAGAGGTGTCATAGCGAATGGGCAATGTTTCAAGCACGAATGACCGGCGCCGAGAAAGCATATTTTGAGGAAATGGGAGTTGATCCTCCAAATTCAACAGCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

215

Amino Acids

24.74

Weight (kDa)

5.99

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 2 - 198 2e-98 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 64, 316, 584
AccB7I CCANNNNNTGG 1 cut(s) 191
AccII CGCG 1 cut(s) 452
AciI CCGC 4 cut(s) 40, 217, 278, 503
AclWI GGATC 4 cut(s) 105, 176, 269, 614
AcsI RAATTY 1 cut(s) 628
AcyI GRCGYC 1 cut(s) 585
AdeI CACNNNGTG 1 cut(s) 293
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 4 cut(s) 191, 385, 485, 527
AgsI TTSAA 3 cut(s) 308, 569, 633
AjnI CCWGG 3 cut(s) 172, 259, 372
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AluBI AGCT 1 cut(s) 638
AluI AGCT 1 cut(s) 638
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 1 cut(s) 250
AlwI GGATC 4 cut(s) 105, 176, 269, 614
Ama87I CYCGRG 2 cut(s) 330, 485
ApoI RAATTY 1 cut(s) 628
AspLEI GCGC 2 cut(s) 454, 587
AspS9I GGNCC 1 cut(s) 376
AvaI CYCGRG 2 cut(s) 330, 485
AvaII GGWCC 1 cut(s) 376
BanI GGYRCC 3 cut(s) 64, 316, 584
Bbv12I GWGCWC 1 cut(s) 520
BccI CCATC 1 cut(s) 474
BciT130I CCWGG 3 cut(s) 174, 261, 374
BcoDI GTCTC 1 cut(s) 250
BfoI RGCGCY 1 cut(s) 588
Bme1390I CCNGG 3 cut(s) 174, 261, 374
Bme18I GGWCC 1 cut(s) 376
BmeT110I CYCGRG 2 cut(s) 330, 485
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 4 cut(s) 66, 318, 491, 586
BmrFI CCNGG 3 cut(s) 174, 261, 374
BmsI GCATC 4 cut(s) 58, 109, 305, 325
BsaAI YACGTR 1 cut(s) 79
BsaBI GATNNNNATC 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 3 cut(s) 379, 493, 521
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 385, 485, 527
Bse118I RCCGGY 1 cut(s) 581
Bse3DI GCAATG 2 cut(s) 461, 567
Bse8I GATNNNNATC 1 cut(s) 96
BseBI CCWGG 3 cut(s) 174, 261, 374
BseDI CCNNGG 3 cut(s) 379, 493, 521
BseGI GGATG 3 cut(s) 49, 97, 340
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 4 cut(s) 191, 385, 485, 527
BseMI GCAATG 2 cut(s) 461, 567
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 1 cut(s) 266
BseYI CCCAGC 1 cut(s) 384
BsgI GTGCAG 2 cut(s) 289, 408
Bsh1236I CGCG 1 cut(s) 452
BshNI GGYRCC 3 cut(s) 64, 316, 584
BsiHKAI GWGCWC 1 cut(s) 520
BsiHKCI CYCGRG 2 cut(s) 330, 485
BsiSI CCGG 1 cut(s) 582
BsiWI CGTACG 1 cut(s) 69
BslI CCNNNNNNNGG 4 cut(s) 191, 385, 485, 527
BsmAI GTCTC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 6
Bso31I GGTCTC 1 cut(s) 250
BsoBI CYCGRG 2 cut(s) 330, 485
Bsp1286I GDGCHC 1 cut(s) 520
Bsp143I GATC 4 cut(s) 97, 181, 274, 619
Bsp19I CCATGG 2 cut(s) 379, 521
BspACI CCGC 4 cut(s) 40, 217, 278, 503
BspCNI CTCAG 1 cut(s) 115
BspFNI CGCG 1 cut(s) 452
BspLI GGNNCC 4 cut(s) 66, 318, 491, 586
BspPI GGATC 4 cut(s) 105, 176, 269, 614
BspQI GCTCTTC 1 cut(s) 508
BspT107I GGYRCC 3 cut(s) 64, 316, 584
BspTNI GGTCTC 1 cut(s) 250
BsrDI GCAATG 2 cut(s) 461, 567
BsrFI RCCGGY 1 cut(s) 581
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 3 cut(s) 379, 493, 521
BssMI GATC 4 cut(s) 97, 181, 274, 619
BssNI GRCGYC 1 cut(s) 585
BssT1I CCWWGG 3 cut(s) 379, 493, 521
Bst2UI CCWGG 3 cut(s) 174, 261, 374
Bst4CI ACNGT 1 cut(s) 69
Bst6I CTCTTC 1 cut(s) 508
BstACI GRCGYC 1 cut(s) 585
BstBAI YACGTR 1 cut(s) 79
BstC8I GCNNGC 2 cut(s) 423, 501
BstDEI CTNAG 2 cut(s) 123, 404
BstDSI CCRYGG 2 cut(s) 379, 521
BstF5I GGATG 3 cut(s) 49, 97, 340
BstFNI CGCG 1 cut(s) 452
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 2 cut(s) 454, 587
BstKTI GATC 4 cut(s) 100, 184, 277, 622
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 4 cut(s) 97, 181, 274, 619
BstMWI GCNNNNNNNGC 3 cut(s) 46, 202, 593
BstNI CCWGG 3 cut(s) 174, 261, 374
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 3 cut(s) 172, 259, 372
BstSNI TACGTA 1 cut(s) 79
BstUI CGCG 1 cut(s) 452
BstX2I RGATCY 2 cut(s) 97, 181
BstYI RGATCY 2 cut(s) 97, 181
BtgI CCRYGG 2 cut(s) 379, 521
BtsCI GGATG 3 cut(s) 49, 97, 340
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 1 cut(s) 277
Cac8I GCNNGC 2 cut(s) 423, 501
CfoI GCGC 2 cut(s) 454, 587
Cfr10I RCCGGY 1 cut(s) 581
Cfr13I GGNCC 1 cut(s) 376
CseI GACGC 1 cut(s) 458
CsiI ACCWGGT 2 cut(s) 259, 372
Csp6I GTAC 1 cut(s) 70
CviAII CATG 7 cut(s) 152, 201, 241, 291, 339, 380, 522
CviJI RGCY 5 cut(s) 172, 196, 384, 421, 638
CviKI_1 RGCY 5 cut(s) 172, 196, 384, 421, 638
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 2 cut(s) 123, 404
DinI GGCGCC 1 cut(s) 586
DpnI GATC 4 cut(s) 99, 183, 276, 621
DpnII GATC 4 cut(s) 97, 181, 274, 619
DraIII CACNNNGTG 1 cut(s) 293
Eam1104I CTCTTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 508
EciI GGCGGA 1 cut(s) 232
Eco105I TACGTA 1 cut(s) 79
Eco130I CCWWGG 3 cut(s) 379, 493, 521
Eco31I GGTCTC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 376
Eco88I CYCGRG 2 cut(s) 330, 485
EcoRII CCWGG 3 cut(s) 172, 259, 372
EcoT14I CCWWGG 3 cut(s) 379, 493, 521
EcoT22I ATGCAT 1 cut(s) 340
EgeI GGCGCC 1 cut(s) 586
EheI GGCGCC 1 cut(s) 586
ErhI CCWWGG 3 cut(s) 379, 493, 521
FaeI CATG 7 cut(s) 155, 204, 244, 294, 342, 383, 525
FatI CATG 7 cut(s) 151, 200, 240, 290, 338, 379, 521
FauNDI CATATG 1 cut(s) 357
FokI GGATG 3 cut(s) 36, 104, 347
GlaI GCGC 2 cut(s) 453, 586
GsaI CCCAGC 1 cut(s) 388
HaeII RGCGCY 1 cut(s) 588
HapII CCGG 1 cut(s) 582
HgaI GACGC 1 cut(s) 458
HhaI GCGC 2 cut(s) 454, 587
Hin1I GRCGYC 1 cut(s) 585
Hin1II CATG 7 cut(s) 155, 204, 244, 294, 342, 383, 525
Hin6I GCGC 2 cut(s) 452, 585
HinP1I GCGC 2 cut(s) 452, 585
HinfI GANTC 2 cut(s) 361, 441
HpaII CCGG 1 cut(s) 582
Hpy166II GTNNAC 2 cut(s) 238, 448
Hpy188I TCNGA 2 cut(s) 12, 366
Hpy188III TCNNGA 5 cut(s) 53, 107, 332, 392, 487
Hpy8I GTNNAC 2 cut(s) 238, 448
HpyAV CCTTC 2 cut(s) 489, 521
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 6 cut(s) 49, 244, 270, 296, 338, 425
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 202, 593
HpyF3I CTNAG 2 cut(s) 123, 404
HpySE526I ACGT 1 cut(s) 78
Hsp92I GRCGYC 1 cut(s) 585
Hsp92II CATG 7 cut(s) 155, 204, 244, 294, 342, 383, 525
HspAI GCGC 2 cut(s) 452, 585
KasI GGCGCC 1 cut(s) 584
Kzo9I GATC 4 cut(s) 97, 181, 274, 619
LguI GCTCTTC 1 cut(s) 508
LweI GCATC 4 cut(s) 58, 109, 305, 325
MabI ACCWGGT 2 cut(s) 259, 372
MaeII ACGT 1 cut(s) 78
MalI GATC 4 cut(s) 99, 183, 276, 621
MboI GATC 4 cut(s) 97, 181, 274, 619
MboII GAAGA 1 cut(s) 525
MflI RGATCY 2 cut(s) 97, 181
MhlI GDGCHC 1 cut(s) 520
MluCI AATT 5 cut(s) 137, 309, 409, 435, 628
Mly113I GGCGCC 1 cut(s) 585
MlyI GAGTC 1 cut(s) 450
MnlI CCTC 6 cut(s) 88, 244, 499, 534, 598, 633
Mph1103I ATGCAT 1 cut(s) 340
MslI CAYNNNNRTG 1 cut(s) 117
MspI CCGG 1 cut(s) 582
MspR9I CCNGG 3 cut(s) 174, 261, 374
Mva1269I GAATGC 1 cut(s) 6
MvaI CCWGG 3 cut(s) 174, 261, 374
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 3 cut(s) 46, 202, 593
NarI GGCGCC 1 cut(s) 585
NcoI CCATGG 2 cut(s) 379, 521
NdeI CATATG 1 cut(s) 357
NdeII GATC 4 cut(s) 97, 181, 274, 619
NlaIII CATG 7 cut(s) 155, 204, 244, 294, 342, 383, 525
NlaIV GGNNCC 4 cut(s) 66, 318, 491, 586
NmeAIII GCCGAG 1 cut(s) 613
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 244
PciSI GCTCTTC 1 cut(s) 508
PctI GAATGC 1 cut(s) 6
PfeI GAWTC 1 cut(s) 361
Pfl23II CGTACG 1 cut(s) 69
PflFI GACNNNGTC 1 cut(s) 374
PflMI CCANNNNNTGG 1 cut(s) 191
PleI GAGTC 1 cut(s) 449
PluTI GGCGCC 1 cut(s) 588
PpsI GAGTC 1 cut(s) 449
Ppu21I YACGTR 1 cut(s) 79
Psp6I CCWGG 3 cut(s) 172, 259, 372
PspFI CCCAGC 1 cut(s) 384
PspGI CCWGG 3 cut(s) 172, 259, 372
PspLI CGTACG 1 cut(s) 69
PspN4I GGNNCC 4 cut(s) 66, 318, 491, 586
PspPI GGNCC 1 cut(s) 376
PsuI RGATCY 2 cut(s) 97, 181
PsyI GACNNNGTC 1 cut(s) 374
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
RseI CAYNNNNRTG 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 508
Sau3AI GATC 4 cut(s) 97, 181, 274, 619
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 1 cut(s) 450
ScrFI CCNGG 3 cut(s) 174, 261, 374
SduI GDGCHC 1 cut(s) 520
SetI ASST 7 cut(s) 81, 178, 262, 322, 375, 545, 640
SexAI ACCWGGT 2 cut(s) 259, 372
SfaNI GCATC 4 cut(s) 58, 109, 305, 325
SfoI GGCGCC 1 cut(s) 586
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 117
SnaBI TACGTA 1 cut(s) 79
Sse9I AATT 5 cut(s) 137, 309, 409, 435, 628
SsiI CCGC 4 cut(s) 40, 217, 278, 503
SspDI GGCGCC 1 cut(s) 584
StyD4I CCNGG 3 cut(s) 172, 259, 372
StyI CCWWGG 3 cut(s) 379, 493, 521
TaaI ACNGT 1 cut(s) 69
TaiI ACGT 1 cut(s) 81
TasI AATT 5 cut(s) 137, 309, 409, 435, 628
TfiI GAWTC 1 cut(s) 361
TscAI CASTG 1 cut(s) 277
TspRI CASTG 1 cut(s) 277
Tth111I GACNNNGTC 1 cut(s) 374
Van91I CCANNNNNTGG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 1 cut(s) 628
XceI RCATGY 1 cut(s) 244
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.