pycom11g04400

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003691
Physical Location & Seq
Reverse (-)
3418670 .. 3419129
460 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 363 bp
ATGGCAAAGACTGCGGTGTCTAGGGCGGCATTCACGGTGGTGCTATTATCGATGCTTGTGACTGCTCCTTACACCGTTAATGCAACGGTAACTTGTGGTCAGGTCGTCACATTACTCACTCCATGCATCCCCTTTGGAGTGTTTGGAGGAGACGTGCCGCCAGATTGTTGTGCCGGCATAAAAGGCCTGCATGATATACAAAACACCGCGGAGGATCGAAGAACTGCGTGCAGCTGCATTCAACAAGGGGCTGCAATGATTCCAGGGTTAGATTATGACCGAGTTAACACGCTTGGGGATCGATGTGGTTCTCCTTGCCCCTACACAGTTTACCCCTCTACTAACTGCTCCGAGGTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

121

Amino Acids

12.58

Weight (kDa)

5.03

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 116 8.6e-09 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 16
AccII CGCG 1 cut(s) 209
AciI CCGC 5 cut(s) 14, 26, 158, 207, 209
AclWI GGATC 2 cut(s) 222, 306
AgsI TTSAA 1 cut(s) 242
AjiI CACGTC 1 cut(s) 154
AjnI CCWGG 1 cut(s) 262
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 1 cut(s) 234
AluI AGCT 1 cut(s) 234
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 2 cut(s) 222, 306
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 3 cut(s) 231, 234, 251
BbvI GCAGC 3 cut(s) 221, 238, 243
BciT130I CCWGG 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 1 cut(s) 21
BisI GCNGC 5 cut(s) 27, 158, 232, 235, 252
BlsI GCNGC 5 cut(s) 28, 159, 233, 236, 253
Bme1390I CCNGG 1 cut(s) 264
BmgBI CACGTC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 42, 135
Bsa29I ATCGAT 2 cut(s) 50, 301
BsaJI CCNNGG 3 cut(s) 207, 263, 351
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse118I RCCGGY 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 264
BseCI ATCGAT 2 cut(s) 50, 301
BseDI CCNNGG 3 cut(s) 207, 263, 351
BseGI GGATG 1 cut(s) 126
BseMI GCAATG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 3 cut(s) 221, 238, 243
BsgI GTGCAG 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 209
BshFI GGCC 1 cut(s) 186
BshVI ATCGAT 2 cut(s) 50, 301
BsiSI CCGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 144
BsmBI CGTCTC 1 cut(s) 144
BsmI GAATGC 2 cut(s) 29, 237
BsnI GGCC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 214, 298
BspACI CCGC 5 cut(s) 14, 26, 158, 207, 209
BspANI GGCC 1 cut(s) 186
BspDI ATCGAT 2 cut(s) 50, 301
BspFNI CGCG 1 cut(s) 209
BspPI GGATC 2 cut(s) 222, 306
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 3 cut(s) 207, 263, 351
BssMI GATC 2 cut(s) 214, 298
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 4 cut(s) 37, 76, 88, 328
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 3 cut(s) 175, 188, 229
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 209
BstKTI GATC 2 cut(s) 217, 301
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 2 cut(s) 214, 298
BstMWI GCNNNNNNNGC 2 cut(s) 11, 183
BstNI CCWGG 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 262
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 3 cut(s) 221, 238, 243
Bsu15I ATCGAT 2 cut(s) 50, 301
BsuRI GGCC 1 cut(s) 186
BsuTUI ATCGAT 2 cut(s) 50, 301
BtgI CCRYGG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 1 cut(s) 126
Cac8I GCNNGC 3 cut(s) 175, 188, 229
Cfr10I RCCGGY 1 cut(s) 173
Cfr42I CCGCGG 1 cut(s) 210
ClaI ATCGAT 2 cut(s) 50, 301
CviAII CATG 2 cut(s) 123, 191
CviJI RGCY 3 cut(s) 186, 234, 251
CviKI_1 RGCY 3 cut(s) 186, 234, 251
DpnI GATC 2 cut(s) 216, 300
DpnII GATC 2 cut(s) 214, 298
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
Eco147I AGGCCT 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 128
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 2 cut(s) 126, 194
FaiI YATR 5 cut(s) 124, 179, 192, 197, 276
FatI CATG 2 cut(s) 122, 190
Fnu4HI GCNGC 5 cut(s) 27, 158, 232, 235, 252
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 5 cut(s) 27, 158, 232, 235, 252
FspBI CTAG 1 cut(s) 21
GluI GCNGC 5 cut(s) 27, 158, 232, 235, 252
HaeIII GGCC 1 cut(s) 186
HapII CCGG 1 cut(s) 174
Hin1II CATG 2 cut(s) 126, 194
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 1 cut(s) 259
HpaI GTTAAC 1 cut(s) 286
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 2 cut(s) 286, 331
Hpy188I TCNGA 1 cut(s) 352
Hpy8I GTNNAC 2 cut(s) 286, 331
HpyCH4III ACNGT 4 cut(s) 37, 76, 88, 328
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 6 cut(s) 83, 126, 190, 231, 237, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 183
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 2 cut(s) 126, 194
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
KspAI GTTAAC 1 cut(s) 286
KspI CCGCGG 1 cut(s) 210
Kzo9I GATC 2 cut(s) 214, 298
LmnI GCTCC 2 cut(s) 70, 353
LpnPI CCDG 6 cut(s) 86, 174, 187, 200, 249, 276
Lsp1109I GCAGC 3 cut(s) 221, 238, 243
LweI GCATC 2 cut(s) 42, 135
MaeI CTAG 1 cut(s) 21
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 3 cut(s) 58, 88, 106
MalI GATC 2 cut(s) 216, 300
MboI GATC 2 cut(s) 214, 298
MboII GAAGA 1 cut(s) 231
MluCI AATT 1 cut(s) 358
MnlI CCTC 4 cut(s) 140, 205, 346, 346
Mph1103I ATGCAT 1 cut(s) 128
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 2 cut(s) 78, 285
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 2 cut(s) 209, 234
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 264
Mva1269I GAATGC 2 cut(s) 29, 237
MvaI CCWGG 1 cut(s) 264
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 11, 183
NaeI GCCGGC 1 cut(s) 175
NdeII GATC 2 cut(s) 214, 298
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 2 cut(s) 126, 194
NmuCI GTSAC 2 cut(s) 58, 106
NsiI ATGCAT 1 cut(s) 128
OliI CACNNNNGTG 1 cut(s) 38
PceI AGGCCT 1 cut(s) 186
PctI GAATGC 2 cut(s) 29, 237
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 5 cut(s) 28, 159, 233, 236, 253
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 234
RseI CAYNNNNRTG 1 cut(s) 38
SacII CCGCGG 1 cut(s) 210
SaqAI TTAA 2 cut(s) 78, 285
SatI GCNGC 5 cut(s) 27, 158, 232, 235, 252
Sau3AI GATC 2 cut(s) 214, 298
ScrFI CCNGG 1 cut(s) 264
SetI ASST 4 cut(s) 105, 156, 236, 357
SfaNI GCATC 2 cut(s) 42, 135
Sfr303I CCGCGG 1 cut(s) 210
SgrBI CCGCGG 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 38
Sse9I AATT 1 cut(s) 358
SseBI AGGCCT 1 cut(s) 186
SsiI CCGC 5 cut(s) 14, 26, 158, 207, 209
SspMI CTAG 1 cut(s) 21
StuI AGGCCT 1 cut(s) 186
StyD4I CCNGG 1 cut(s) 262
TaaI ACNGT 4 cut(s) 37, 76, 88, 328
TaiI ACGT 1 cut(s) 156
TaqI TCGA 3 cut(s) 50, 217, 301
TaqII GACCGA 1 cut(s) 294
TasI AATT 1 cut(s) 358
TauI GCSGC 2 cut(s) 29, 160
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 2 cut(s) 78, 285
Tru9I TTAA 2 cut(s) 78, 285
TseFI GTSAC 2 cut(s) 58, 106
TseI GCWGC 3 cut(s) 231, 234, 251
Tsp45I GTSAC 2 cut(s) 58, 106
XspI CTAG 1 cut(s) 21
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.