pycom11g05750

Pyruvate decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001627
Physical Location & Seq
Reverse (-)
4426030 .. 4426448
419 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 330 bp
ATGCAATATGGATCGATTGGTTGGTCTGTTGGTGCAACTCTAGGCTATGCACAGTCAGTTCCGAAGAAGCGCGTCATTGCTTGCATCGGTGATGGGAGCTTCCAGGTTCAAATTAATGCTTCGACCAGCGTGACTGCACAAGATGTGTCAACAATGATTCGATGCAATCAAAAGAGCATCATCTTCCTCATAAACAATGGCGGATACACCATAGAAGTTGAAATCCATGACGGCCCTTACAATGTGATCAAGAACTGGAACTACACTGGTTTGGTGGACGCAATCCACAACGGCGAAGGAAACTGCTGGACAACAAAGGTTAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

110

Amino Acids

11.82

Weight (kDa)

6.69

Isoelectric Point (pI)

22.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_C PF02775 3 - 71 1e-08 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 72
AciI CCGC 1 cut(s) 201
AclWI GGATC 1 cut(s) 19
AgsI TTSAA 2 cut(s) 110, 221
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 1 cut(s) 99
AluI AGCT 1 cut(s) 99
AlwI GGATC 1 cut(s) 19
AoxI GGCC 1 cut(s) 232
AseI ATTAAT 1 cut(s) 114
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 1 cut(s) 233
AsuHPI GGTGA 1 cut(s) 101
BccI CCATC 1 cut(s) 86
BceAI ACGGC 2 cut(s) 247, 307
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 197
BclI TGATCA 1 cut(s) 246
BfaI CTAG 1 cut(s) 41
BfuI GTATCC 1 cut(s) 197
Bme1390I CCNGG 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 233
BmrFI CCNGG 1 cut(s) 104
BmsI GCATC 3 cut(s) 93, 152, 186
Bsa29I ATCGAT 1 cut(s) 14
Bse1I ACTGG 2 cut(s) 260, 271
Bse3DI GCAATG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 104
BseCI ATCGAT 1 cut(s) 14
BseMI GCAATG 1 cut(s) 75
BseNI ACTGG 2 cut(s) 260, 271
BsgI GTGCAG 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 1 cut(s) 234
BshVI ATCGAT 1 cut(s) 14
BsnI GGCC 1 cut(s) 234
Bsp143I GATC 2 cut(s) 11, 246
BspACI CCGC 1 cut(s) 201
BspANI GGCC 1 cut(s) 234
BspDI ATCGAT 1 cut(s) 14
BspFNI CGCG 1 cut(s) 72
BspPI GGATC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 75
BsrI ACTGG 2 cut(s) 260, 271
BssMI GATC 2 cut(s) 11, 246
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 82
BstFNI CGCG 1 cut(s) 72
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 2 cut(s) 14, 249
BstMBI GATC 2 cut(s) 11, 246
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 102
BstUI CGCG 1 cut(s) 72
Bsu15I ATCGAT 1 cut(s) 14
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 14
BtsIMutI CAGTG 1 cut(s) 264
Cac8I GCNNGC 1 cut(s) 82
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 233
ClaI ATCGAT 1 cut(s) 14
CseI GACGC 2 cut(s) 61, 287
CviAII CATG 1 cut(s) 227
CviJI RGCY 3 cut(s) 45, 99, 234
CviKI_1 RGCY 3 cut(s) 45, 99, 234
DpnI GATC 2 cut(s) 13, 248
DpnII GATC 2 cut(s) 11, 246
EciI GGCGGA 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 102
FaeI CATG 1 cut(s) 230
FaiI YATR 5 cut(s) 9, 48, 191, 212, 228
FatI CATG 1 cut(s) 226
FbaI TGATCA 1 cut(s) 246
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 71
HaeIII GGCC 1 cut(s) 234
HgaI GACGC 2 cut(s) 61, 287
HhaI GCGC 1 cut(s) 72
Hin1II CATG 1 cut(s) 230
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HinfI GANTC 1 cut(s) 157
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 150, 277
Hpy188I TCNGA 1 cut(s) 63
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 2 cut(s) 150, 277
HpyAV CCTTC 1 cut(s) 290
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4V TGCA 6 cut(s) 4, 35, 50, 84, 137, 165
Hsp92II CATG 1 cut(s) 230
HspAI GCGC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 2 cut(s) 11, 246
LmnI GCTCC 1 cut(s) 96
LpnPI CCDG 6 cut(s) 89, 116, 139, 241, 252, 292
LweI GCATC 3 cut(s) 93, 152, 186
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 130
MalI GATC 2 cut(s) 13, 248
MboI GATC 2 cut(s) 11, 246
MboII GAAGA 2 cut(s) 76, 175
MluCI AATT 1 cut(s) 111
MnlI CCTC 1 cut(s) 197
MseI TTAA 1 cut(s) 114
MspR9I CCNGG 1 cut(s) 104
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 1 cut(s) 72
NdeII GATC 2 cut(s) 11, 246
NlaIII CATG 1 cut(s) 230
NmuCI GTSAC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 157
PshBI ATTAAT 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspPI GGNCC 1 cut(s) 233
PsrI GAACNNNNNNTAC 2 cut(s) 245, 277
SaqAI TTAA 1 cut(s) 114
Sau3AI GATC 2 cut(s) 11, 246
Sau96I GGNCC 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 104
SetI ASST 3 cut(s) 101, 108, 321
SfaNI GCATC 3 cut(s) 93, 152, 186
Sse9I AATT 1 cut(s) 111
SsiI CCGC 1 cut(s) 201
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 54
TaqI TCGA 3 cut(s) 14, 122, 160
TasI AATT 1 cut(s) 111
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 1 cut(s) 130
Tsp45I GTSAC 1 cut(s) 130
TspRI CASTG 1 cut(s) 271
VspI ATTAAT 1 cut(s) 114
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.