pycom11g05760

Pyruvate decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001627
Physical Location & Seq
Reverse (-)
4427400 .. 4428450
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
ATGGCCACCAGCATTGGCTCTCTAGACTCATCAAAACCCACCAACAACAACGTCGGCTGCCCTCCTCAAAACGGTACTGCCACCATCCACGACTCCTCCCACCCTCCGTCCACCATTGCCTCCGCTGACTCCACTCTTGGCCGCCACTTAGCGCGCCGCCTGGTGGAGATAGGGGTGGGGGACGTGTTCACTGTCCCCGGGGATTTTAACTTAACCCTATTAGACCACCTCATTGCTGAGCGGAAGCTCACCAACATCGGCTGCTGCAATGAGCTCAATGCCGGGTACGCCGCTGATGGATATGCCCGGGAGCGTGGCGTTGGGGCTTGCGTTGTCACGTTTACCGTGGGTGGGCTTAGTGTTCTCAACGCCATTGCTGGAGCTTACAGTGAGAACCTTCCTGTTATTTGTGTTGTTGGAGGGCCAAACACAAATGATTATGGCACCAACAGAATTCTTCACCACACCATTGGCTTGCCGGATTTCAGCCAAGAACTCAGATGCTTCCAGACTGTCACTTGCTATCAAGCTGTGGTAAACAACTTGGATGATGCACATGAACAAATTGATACTGCCATTTCTACTGCTCTCAAAGAAAGCAAGCCTGTCTATATCAGCATTAGCTGCAACTTGCCTGGCATTCCACATCCAGCTTTTAGCACACACCCCATTAAATTTTCCATCTCACCTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

232

Amino Acids

24.43

Weight (kDa)

5.79

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 45 - 150 1.1e-28 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 443
AccBSI CCGCTC 1 cut(s) 241
AccII CGCG 1 cut(s) 154
AciI CCGC 5 cut(s) 123, 142, 157, 241, 291
AcoI YGGCCR 2 cut(s) 3, 139
AcsI RAATTY 2 cut(s) 453, 674
AfaI GTAC 2 cut(s) 76, 287
AfiI CCNNNNNNNGG 3 cut(s) 71, 163, 351
AflIII ACRYGT 1 cut(s) 183
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 2 cut(s) 159, 634
AluBI AGCT 6 cut(s) 247, 274, 383, 530, 624, 653
AluI AGCT 6 cut(s) 247, 274, 383, 530, 624, 653
Alw21I GWGCWC 1 cut(s) 276
Ama87I CYCGRG 2 cut(s) 197, 306
AoxI GGCC 3 cut(s) 3, 139, 422
ApeKI GCWGC 4 cut(s) 57, 261, 264, 624
ApoI RAATTY 2 cut(s) 453, 674
AspA2I CCTAGG 1 cut(s) 689
AspLEI GCGC 2 cut(s) 154, 156
AspS9I GGNCC 1 cut(s) 422
AsuC2I CCSGG 5 cut(s) 198, 199, 283, 307, 308
AsuHPI GGTGA 3 cut(s) 241, 452, 678
AvaI CYCGRG 2 cut(s) 197, 306
AvrII CCTAGG 1 cut(s) 689
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 443
BanII GRGCYC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 4 cut(s) 44, 248, 251, 611
BccI CCATC 3 cut(s) 92, 290, 689
BciT130I CCWGG 2 cut(s) 161, 636
BcnI CCSGG 5 cut(s) 198, 199, 283, 307, 308
BfaI CTAG 2 cut(s) 23, 690
BisI GCNGC 7 cut(s) 58, 142, 157, 262, 265, 291, 625
BlnI CCTAGG 1 cut(s) 689
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 7 cut(s) 59, 143, 158, 263, 266, 292, 626
Bme1390I CCNGG 7 cut(s) 161, 198, 199, 283, 307, 308, 636
BmeT110I CYCGRG 2 cut(s) 197, 306
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 7 cut(s) 161, 198, 199, 283, 307, 308, 636
BmsI GCATC 2 cut(s) 491, 541
BpmI CTGGAG 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 237
BpuMI CCSGG 5 cut(s) 198, 199, 283, 307, 308
BsaJI CCNNGG 6 cut(s) 196, 197, 198, 306, 345, 689
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 163, 351
Bse3DI GCAATG 4 cut(s) 114, 231, 274, 372
BseBI CCWGG 2 cut(s) 161, 636
BseDI CCNNGG 6 cut(s) 196, 197, 198, 306, 345, 689
BseGI GGATG 3 cut(s) 84, 553, 646
BseLI CCNNNNNNNGG 3 cut(s) 71, 163, 351
BseMI GCAATG 4 cut(s) 114, 231, 274, 372
BseMII CTCAG 2 cut(s) 228, 511
BsePI GCGCGC 1 cut(s) 152
BseRI GAGGAG 2 cut(s) 54, 85
BseXI GCAGC 4 cut(s) 44, 248, 251, 611
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 3 cut(s) 5, 141, 424
BshNI GGYRCC 1 cut(s) 443
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 2 cut(s) 197, 306
BsiSI CCGG 4 cut(s) 198, 282, 307, 479
BslFI GGGAC 2 cut(s) 179, 194
BslI CCNNNNNNNGG 3 cut(s) 71, 163, 351
BsmFI GGGAC 2 cut(s) 179, 194
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 3 cut(s) 5, 141, 424
BsoBI CYCGRG 2 cut(s) 197, 306
Bsp1286I GDGCHC 1 cut(s) 276
Bsp1720I GCTNAGC 1 cut(s) 237
BspACI CCGC 5 cut(s) 123, 142, 157, 241, 291
BspANI GGCC 3 cut(s) 5, 141, 424
BspCNI CTCAG 2 cut(s) 229, 510
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 1 cut(s) 445
BspT107I GGYRCC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 241
BsrDI GCAATG 4 cut(s) 114, 231, 274, 372
BssECI CCNNGG 6 cut(s) 196, 197, 198, 306, 345, 689
BssHII GCGCGC 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 689
Bst2UI CCWGG 2 cut(s) 161, 636
Bst4CI ACNGT 5 cut(s) 74, 193, 346, 389, 514
BstAPI GCANNNNNTGC 1 cut(s) 624
BstC8I GCNNGC 4 cut(s) 154, 328, 476, 602
BstDEI CTNAG 4 cut(s) 148, 237, 356, 497
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 3 cut(s) 84, 553, 646
BstFNI CGCG 1 cut(s) 154
BstHHI GCGC 2 cut(s) 154, 156
BstMWI GCNNNNNNNGC 2 cut(s) 287, 624
BstNI CCWGG 2 cut(s) 161, 636
BstSCI CCNGG 7 cut(s) 159, 196, 197, 281, 305, 306, 634
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 4 cut(s) 44, 248, 251, 611
BstXI CCANNNNNNTGG 1 cut(s) 470
BsuRI GGCC 3 cut(s) 5, 141, 424
BtgI CCRYGG 1 cut(s) 345
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 3 cut(s) 84, 553, 646
BtsIMutI CAGTG 2 cut(s) 189, 394
Cac8I GCNNGC 4 cut(s) 154, 328, 476, 602
CfoI GCGC 2 cut(s) 154, 156
Cfr13I GGNCC 1 cut(s) 422
Cfr9I CCCGGG 2 cut(s) 197, 306
Csp6I GTAC 2 cut(s) 75, 286
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 1 cut(s) 557
CviQI GTAC 2 cut(s) 75, 286
DdeI CTNAG 4 cut(s) 148, 237, 356, 497
EaeI YGGCCR 2 cut(s) 3, 139
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 689
Eco24I GRGCYC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 274
Eco88I CYCGRG 2 cut(s) 197, 306
EcoICRI GAGCTC 1 cut(s) 274
EcoRI GAATTC 1 cut(s) 453
EcoRII CCWGG 2 cut(s) 159, 634
EcoT14I CCWWGG 1 cut(s) 689
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 689
FaeI CATG 1 cut(s) 560
FaiI YATR 4 cut(s) 303, 441, 558, 612
FaqI GGGAC 2 cut(s) 179, 194
FatI CATG 1 cut(s) 556
Fnu4HI GCNGC 7 cut(s) 58, 142, 157, 262, 265, 291, 625
FokI GGATG 3 cut(s) 71, 560, 633
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 7 cut(s) 58, 142, 157, 262, 265, 291, 625
FspBI CTAG 2 cut(s) 23, 690
GlaI GCGC 2 cut(s) 153, 155
GluI GCNGC 7 cut(s) 58, 142, 157, 262, 265, 291, 625
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 3 cut(s) 5, 141, 424
HapII CCGG 4 cut(s) 198, 282, 307, 479
HhaI GCGC 2 cut(s) 154, 156
Hin1II CATG 1 cut(s) 560
Hin6I GCGC 2 cut(s) 152, 154
HinP1I GCGC 2 cut(s) 152, 154
HinfI GANTC 3 cut(s) 26, 92, 128
HpaII CCGG 4 cut(s) 198, 282, 307, 479
HphI GGTGA 3 cut(s) 241, 452, 678
Hpy166II GTNNAC 4 cut(s) 111, 189, 342, 538
Hpy188I TCNGA 1 cut(s) 500
Hpy188III TCNNGA 2 cut(s) 23, 508
Hpy8I GTNNAC 4 cut(s) 111, 189, 342, 538
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 1 cut(s) 407
HpyCH4III ACNGT 5 cut(s) 74, 193, 346, 389, 514
HpyCH4IV ACGT 3 cut(s) 51, 183, 338
HpyCH4V TGCA 3 cut(s) 267, 554, 627
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 624
HpyF3I CTNAG 4 cut(s) 148, 237, 356, 497
HpySE526I ACGT 3 cut(s) 51, 183, 338
Hsp92II CATG 1 cut(s) 560
HspAI GCGC 2 cut(s) 152, 154
LmnI GCTCC 2 cut(s) 310, 380
Lsp1109I GCAGC 4 cut(s) 44, 248, 251, 611
LweI GCATC 2 cut(s) 491, 541
MaeI CTAG 2 cut(s) 23, 690
MaeII ACGT 3 cut(s) 51, 183, 338
MaeIII GTNAC 2 cut(s) 334, 514
MbiI CCGCTC 1 cut(s) 241
MboII GAAGA 1 cut(s) 449
MhlI GDGCHC 1 cut(s) 276
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 453, 564, 674
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 20, 86, 122
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 7 cut(s) 72, 75, 106, 114, 130, 239, 413
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 207, 212, 672
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 125, 293
MspI CCGG 4 cut(s) 198, 282, 307, 479
MspR9I CCNGG 7 cut(s) 161, 198, 199, 283, 307, 308, 636
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 2 cut(s) 161, 636
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 2 cut(s) 287, 624
NciI CCSGG 5 cut(s) 198, 199, 283, 307, 308
NlaIII CATG 1 cut(s) 560
NlaIV GGNNCC 1 cut(s) 445
NmuCI GTSAC 2 cut(s) 334, 514
PauI GCGCGC 1 cut(s) 152
PctI GAATGC 1 cut(s) 639
PkrI GCNGC 7 cut(s) 59, 143, 158, 263, 266, 292, 626
PleI GAGTC 3 cut(s) 20, 86, 122
PpsI GAGTC 3 cut(s) 20, 86, 122
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 159, 634
PspGI CCWGG 2 cut(s) 159, 634
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 1 cut(s) 422
PteI GCGCGC 1 cut(s) 152
RsaI GTAC 2 cut(s) 76, 287
RsaNI GTAC 2 cut(s) 75, 286
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 3 cut(s) 207, 212, 672
SatI GCNGC 7 cut(s) 58, 142, 157, 262, 265, 291, 625
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 3 cut(s) 20, 86, 122
ScrFI CCNGG 7 cut(s) 161, 198, 199, 283, 307, 308, 636
SduI GDGCHC 1 cut(s) 276
SfaNI GCATC 2 cut(s) 491, 541
SmaI CCCGGG 2 cut(s) 199, 308
Sse9I AATT 3 cut(s) 453, 564, 674
SsiI CCGC 5 cut(s) 123, 142, 157, 241, 291
SspMI CTAG 2 cut(s) 23, 690
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 7 cut(s) 159, 196, 197, 281, 305, 306, 634
StyI CCWWGG 1 cut(s) 689
TaaI ACNGT 5 cut(s) 74, 193, 346, 389, 514
TaiI ACGT 3 cut(s) 54, 186, 341
TasI AATT 3 cut(s) 453, 564, 674
TauI GCSGC 3 cut(s) 144, 159, 293
Tru1I TTAA 3 cut(s) 207, 212, 672
Tru9I TTAA 3 cut(s) 207, 212, 672
TscAI CASTG 2 cut(s) 196, 394
TseFI GTSAC 2 cut(s) 334, 514
TseI GCWGC 4 cut(s) 57, 261, 264, 624
Tsp45I GTSAC 2 cut(s) 334, 514
TspDTI ATGAA 1 cut(s) 573
TspGWI ACGGA 1 cut(s) 96
TspMI CCCGGG 2 cut(s) 197, 306
TspRI CASTG 2 cut(s) 196, 394
XapI RAATTY 2 cut(s) 453, 674
XbaI TCTAGA 1 cut(s) 22
XmaI CCCGGG 2 cut(s) 197, 306
XmaJI CCTAGG 1 cut(s) 689
XspI CTAG 2 cut(s) 23, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.