pycom11g10870

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002350
Physical Location & Seq
Reverse (-)
9233618 .. 9236688
3071 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
ATGGCTGCTGATATGGGAGGTTCTTATGGGTCTACCCTGCGATATAAGAGTGTGGAGCGAATGAAGGCCGTTGGAAAGCATTCTCTTCTTGGTGCAAGTGGAGAAATAAGTGACTTTCAGGAGCTATTACGTTATCTTGATGAACTCATCCTATACGACAACATGTGGGATGATGGGAACTCTTTGGGACCGAAAGAGGTACACAACTATCTGACTCGTGTGATGTACAATAGGCGTAACAAGTTCAATCCACTGTGGAACTCTCTTGTCCTTGGTGGAGTAAAAAAGGGACAGAAGTATCTTGGCATGGTTAGCATGATAGGCGTGAATTTCGAGGACAATCATGTAGCAACTGGGTTTGGAAATCACCTTGCACGGCCTATTCTTCGTGATGAGTGGCATGAGAACTTGACTTTCGAAGAAGGTGTAAAGCTGCTGGAGAAATGCATGCGTGTCCTTCTCTATCGTGATCGGTCTGCTGTGAACAAGCTTCAGATATCTAAGATCACTGAAGAAGGCGTGACTATCTCGCAGCCCTACTCTTTGAAGACTTTCTGGGGTTTCTCCGCTTTCGAAAATCCAACACTGGGAGCTGAAGGATCATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

203

Amino Acids

22.96

Weight (kDa)

6.97

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 1 - 167 2.5e-24 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 32
AciI CCGC 1 cut(s) 567
AclWI GGATC 1 cut(s) 607
AcsI RAATTY 1 cut(s) 328
AcuI CTGAAG 2 cut(s) 476, 531
AfaI GTAC 2 cut(s) 201, 227
AfiI CCNNNNNNNGG 1 cut(s) 587
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 2 cut(s) 247, 547
AluBI AGCT 4 cut(s) 124, 433, 490, 593
AluI AGCT 4 cut(s) 124, 433, 490, 593
AlwI GGATC 1 cut(s) 607
AoxI GGCC 2 cut(s) 66, 377
ApeKI GCWGC 3 cut(s) 5, 433, 532
ApoI RAATTY 1 cut(s) 328
Asp700I GAANNNNTTC 2 cut(s) 79, 551
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 359
AsuII TTCGAA 2 cut(s) 417, 573
AvaII GGWCC 1 cut(s) 188
BaeI ACNNNNGTAYC 2 cut(s) 281, 314
BauI CACGAG 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 554
BbvI GCAGC 2 cut(s) 420, 544
BccI CCATC 1 cut(s) 167
BceAI ACGGC 2 cut(s) 53, 392
BisI GCNGC 3 cut(s) 6, 434, 533
BlsI GCNGC 3 cut(s) 7, 435, 534
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 189
BmrI ACTGGG 2 cut(s) 363, 596
BmuI ACTGGG 2 cut(s) 363, 596
BpiI GAAGAC 1 cut(s) 554
BpmI CTGGAG 1 cut(s) 458
Bpu14I TTCGAA 2 cut(s) 417, 573
BsaJI CCNNGG 1 cut(s) 271
Bsc4I CCNNNNNNNGG 1 cut(s) 587
Bse1I ACTGG 2 cut(s) 358, 591
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 2 cut(s) 147, 175
BseLI CCNNNNNNNGG 1 cut(s) 587
BseNI ACTGG 2 cut(s) 358, 591
BseXI GCAGC 2 cut(s) 420, 544
BshFI GGCC 2 cut(s) 68, 379
BslFI GGGAC 2 cut(s) 201, 303
BslI CCNNNNNNNGG 1 cut(s) 587
BsmFI GGGAC 2 cut(s) 201, 303
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 2 cut(s) 68, 379
Bsp119I TTCGAA 2 cut(s) 417, 573
Bsp1407I TGTACA 1 cut(s) 225
Bsp143I GATC 3 cut(s) 469, 504, 599
BspACI CCGC 1 cut(s) 567
BspANI GGCC 2 cut(s) 68, 379
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 1 cut(s) 607
BspT104I TTCGAA 2 cut(s) 417, 573
BsrGI TGTACA 1 cut(s) 225
BsrI ACTGG 2 cut(s) 358, 591
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 3 cut(s) 469, 504, 599
BssSI CACGAG 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 271
Bst2BI CACGAG 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 90
BstAUI TGTACA 1 cut(s) 225
BstBI TTCGAA 2 cut(s) 417, 573
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 1 cut(s) 501
BstF5I GGATG 2 cut(s) 147, 175
BstKTI GATC 3 cut(s) 472, 507, 602
BstMBI GATC 3 cut(s) 469, 504, 599
BstMWI GCNNNNNNNGC 2 cut(s) 312, 321
BstNSI RCATGY 2 cut(s) 166, 451
BstV1I GCAGC 2 cut(s) 420, 544
BstV2I GAAGAC 1 cut(s) 554
BsuRI GGCC 2 cut(s) 68, 379
BtsCI GGATG 2 cut(s) 147, 175
BtsIMutI CAGTG 3 cut(s) 251, 507, 584
Cac8I GCNNGC 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 200, 226
CviAII CATG 7 cut(s) 163, 307, 316, 344, 401, 448, 603
CviJI RGCY 8 cut(s) 5, 68, 124, 379, 433, 490, 535, 593
CviKI_1 RGCY 8 cut(s) 5, 68, 124, 379, 433, 490, 535, 593
CviQI GTAC 2 cut(s) 200, 226
DdeI CTNAG 1 cut(s) 501
DpnI GATC 3 cut(s) 471, 506, 601
DpnII GATC 3 cut(s) 469, 504, 599
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 271
Eco32I GATATC 1 cut(s) 498
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 2 cut(s) 476, 531
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 7 cut(s) 166, 310, 319, 347, 404, 451, 606
FaqI GGGAC 2 cut(s) 201, 303
FatI CATG 7 cut(s) 162, 306, 315, 343, 400, 447, 602
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 3 cut(s) 6, 434, 533
FokI GGATG 2 cut(s) 134, 182
Fsp4HI GCNGC 3 cut(s) 6, 434, 533
GluI GCNGC 3 cut(s) 6, 434, 533
GsuI CTGGAG 1 cut(s) 458
HaeIII GGCC 2 cut(s) 68, 379
Hin1II CATG 7 cut(s) 166, 310, 319, 347, 404, 451, 606
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 1 cut(s) 214
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 3 cut(s) 33, 202, 484
Hpy188I TCNGA 2 cut(s) 213, 495
Hpy188III TCNNGA 4 cut(s) 119, 137, 389, 467
Hpy8I GTNNAC 3 cut(s) 33, 202, 484
HpyAV CCTTC 5 cut(s) 58, 416, 467, 509, 590
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 3 cut(s) 95, 374, 447
HpyF10VI GCNNNNNNNGC 2 cut(s) 312, 321
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 7 cut(s) 166, 310, 319, 347, 404, 451, 606
Kzo9I GATC 3 cut(s) 469, 504, 599
LmnI GCTCC 3 cut(s) 55, 121, 590
LpnPI CCDG 6 cut(s) 50, 104, 339, 422, 541, 572
Lsp1109I GCAGC 2 cut(s) 420, 544
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 3 cut(s) 110, 236, 520
MalI GATC 3 cut(s) 471, 506, 601
MboI GATC 3 cut(s) 469, 504, 599
MboII GAAGA 5 cut(s) 77, 377, 431, 524, 559
MluCI AATT 1 cut(s) 328
MlyI GAGTC 1 cut(s) 208
MmeI TCCRAC 2 cut(s) 52, 605
MnlI CCTC 3 cut(s) 11, 190, 328
Mph1103I ATGCAT 1 cut(s) 449
MroXI GAANNNNTTC 2 cut(s) 79, 551
Mva1269I GAATGC 1 cut(s) 79
MwoI GCNNNNNNNGC 2 cut(s) 312, 321
NdeII GATC 3 cut(s) 469, 504, 599
NlaIII CATG 7 cut(s) 166, 310, 319, 347, 404, 451, 606
NlaIV GGNNCC 1 cut(s) 189
NmuCI GTSAC 2 cut(s) 110, 520
NsiI ATGCAT 1 cut(s) 449
NspI RCATGY 2 cut(s) 166, 451
NspV TTCGAA 2 cut(s) 417, 573
PaeI GCATGC 1 cut(s) 451
PciI ACATGT 1 cut(s) 162
PctI GAATGC 1 cut(s) 79
PdmI GAANNNNTTC 2 cut(s) 79, 551
PkrI GCNGC 3 cut(s) 7, 435, 534
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PscI ACATGT 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 188
RsaI GTAC 2 cut(s) 201, 227
RsaNI GTAC 2 cut(s) 200, 226
SatI GCNGC 3 cut(s) 6, 434, 533
Sau3AI GATC 3 cut(s) 469, 504, 599
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 1 cut(s) 208
SetI ASST 9 cut(s) 22, 126, 133, 201, 372, 427, 435, 492, 595
SfuI TTCGAA 2 cut(s) 417, 573
SinI GGWCC 1 cut(s) 188
SphI GCATGC 1 cut(s) 451
Sse9I AATT 1 cut(s) 328
SsiI CCGC 1 cut(s) 567
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 1 cut(s) 255
TaiI ACGT 1 cut(s) 133
TaqI TCGA 3 cut(s) 333, 417, 573
TaqII GACCGA 2 cut(s) 205, 462
TasI AATT 1 cut(s) 328
TatI WGTACW 1 cut(s) 225
TscAI CASTG 3 cut(s) 258, 514, 591
TseFI GTSAC 2 cut(s) 110, 520
TseI GCWGC 3 cut(s) 5, 433, 532
Tsp45I GTSAC 2 cut(s) 110, 520
TspDTI ATGAA 2 cut(s) 77, 156
TspRI CASTG 3 cut(s) 258, 514, 591
VpaK11BI GGWCC 1 cut(s) 188
XapI RAATTY 1 cut(s) 328
XceI RCATGY 2 cut(s) 166, 451
XmiI GTMKAC 1 cut(s) 32
XmnI GAANNNNTTC 2 cut(s) 79, 551
Zsp2I ATGCAT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.