pycom11g10930

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002520
Physical Location & Seq
Reverse (-)
9336416 .. 9341943
5528 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1599 bp
ATGGCTTCTGCAGGATCAGCATCCAGCCGTTCACTTCAAGAAACCCCCACCTGGGCTTTGGCAGCAGTTTGTTTTGTGTTCATTTTTCTCTCTCTATTTATAGAGCACTTGATTCATCTCATTAGCAAGTGGCTCAAAAAACACAGAAAAACCGCCTTGTTCGAGGCCGTAGAGAAGCTCAAATCAGTGCTTATACTCCTGGGATTTATGTCACTAATTTTGACAGTATCACAAAGATCGATATCCAAAATATGCATACCCACAAAAGTTGCATTCACCATGCTTCCCTGCAGAAAAAATGCACCAACAAAAACCACCAAAGCACTTGGTTTTGAGCAAATAACAATAGAAAGAATGCTTGCAGCAGCCGATACCAGTGAGGATCACTGTGAATCCCAGGGAAAGACCTCATTTATATCTCAAGGGGGGCTCAATCAGCTCAACAATTTCATCTTCGTTTTGGCAGTTATGCAGATTGTGTATAGTGTTCTCACCATGGCTTTAGGAAGGGCCAAGATGAGGCGTTGGGAAGCATGGGAAAAAGAAACTCAGACAGTGGAATACCAAGTGACAAATGATCCTAATCGGTTTAGGTTTACAAGGCAGACTACATTTGCTCTTCGACATATGAGTTCTTGTACAGGAAGAGGAACCACTGCTCAACTGTGGATTAGGTGCTTCTTCCGGCAGTTCTTTGATTCAGTGGACAAAGTTGACTACCTCACTTTGCGCCACGGTTTCATTTCAACTCATTTGTCCACAAACTACAATGCATTCGATTTCCAAAAGTATATACAACGATCACTGGACAATGATTTTAGAGCTGTGGTCGGAATCAGCCCTTTCCTGTGGTTTATTGTCGTCATCTTCATCCTCCTTGATGTCCATGGTTGGCAGGTGTATCTCTTGGTATCATTTGTGCCACTAATTATAGTTCTAGTTCTTGGAACCAAACTTGAAGTAGTAGTAGCAAACATGGCTCATCAACTTCATGACCAGCATCATGTGATTAAAGGAACTCCTCTGGTGCAACCAAAAGACAGTCACTTCTGGTTCAGTCAACCACAGTTCGTCTTGACTCTTTTACATCTCACTTTGTTTATGAATGCCTTTGAACTTGCTTTCTTTATATGGGTCACGATGCAATTTGGGATACACTCCTGCTACCACGAACACATACATATTATAATCGTACGAGTGGTTTTAGCGGTGACGGTTCAAGTGATGTGCAGTTACATTACTCTTCCCCTCTATGCTCTAGTGACCCAGATGGGATCAAATTTCAAGAGTGCAGCAGTGCTTGAAGAGCAAACAACAAATGTTATAAAGCAGTGGCACGCTGACGTGAAGAGAAAGAGGAAAAATAAATCACACTGTTCACAATCTGGTCACGACGAAAATTCGTCTAGAATCATGAATTCCCCTGATTTCTCTTCACATCGTCCCCAATTAACTTTCGCCGAAATGAACCAGAGTCATCCTAGAGAAACTGAGAATTTTCATGATAACCAAGAAATTGTTGAGGATCATCAAGAAAGGGCTGGCCAAAATGAAATTGAATTAGCCAGCAATGCGTCATCTACTGAGGTACATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

533

Amino Acids

61.0

Weight (kDa)

8.75

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 10 - 470 5.6e-191 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1187, 1325
AarI CACCTGC 1 cut(s) 886
Acc36I ACCTGC 1 cut(s) 886
AciI CCGC 2 cut(s) 153, 1208
AclWI GGATC 5 cut(s) 22, 390, 572, 1282, 1533
AcoI YGGCCR 1 cut(s) 1543
AcsI RAATTY 4 cut(s) 1279, 1399, 1417, 1495
AfaI GTAC 3 cut(s) 640, 1194, 1590
AfiI CCNNNNNNNGG 3 cut(s) 51, 52, 519
AgsI TTSAA 8 cut(s) 38, 747, 959, 1115, 1220, 1285, 1304, 1559
AjiI CACGTC 1 cut(s) 1345
AjnI CCWGG 3 cut(s) 50, 198, 396
AluBI AGCT 3 cut(s) 178, 439, 824
AluI AGCT 3 cut(s) 178, 439, 824
Alw21I GWGCWC 1 cut(s) 108
AlwI GGATC 5 cut(s) 22, 390, 572, 1282, 1533
AoxI GGCC 3 cut(s) 165, 510, 1543
ApeKI GCWGC 4 cut(s) 62, 362, 365, 1292
ApoI RAATTY 4 cut(s) 1279, 1399, 1417, 1495
AspLEI GCGC 1 cut(s) 732
AspS9I GGNCC 1 cut(s) 510
AsuHPI GGTGA 3 cut(s) 268, 484, 1222
BalI TGGCCA 1 cut(s) 1545
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 4 cut(s) 74, 374, 377, 1304
BccI CCATC 1 cut(s) 1264
BceAI ACGGC 2 cut(s) 12, 152
BciT130I CCWGG 3 cut(s) 52, 200, 398
BciVI GTATCC 1 cut(s) 1146
BfaI CTAG 4 cut(s) 938, 1259, 1407, 1482
BfmI CTRYAG 2 cut(s) 9, 289
BfuAI ACCTGC 1 cut(s) 886
BfuI GTATCC 1 cut(s) 1146
BisI GCNGC 4 cut(s) 63, 363, 366, 1293
BlsI GCNGC 4 cut(s) 64, 364, 367, 1294
Bme1390I CCNGG 3 cut(s) 52, 200, 398
BmgBI CACGTC 1 cut(s) 1345
BmgT120I GGNCC 1 cut(s) 510
BmiI GGNNCC 2 cut(s) 652, 949
BmrFI CCNGG 3 cut(s) 52, 200, 398
BmsI GCATC 3 cut(s) 29, 1009, 1131
BpuEI CTTGAG 1 cut(s) 405
Bsa29I ATCGAT 1 cut(s) 239
BsaBI GATNNNNATC 3 cut(s) 19, 241, 582
BsaJI CCNNGG 7 cut(s) 51, 199, 396, 397, 495, 733, 886
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 52, 519
Bse1I ACTGG 2 cut(s) 375, 810
Bse3DI GCAATG 1 cut(s) 1576
Bse8I GATNNNNATC 3 cut(s) 19, 241, 582
BseBI CCWGG 3 cut(s) 52, 200, 398
BseCI ATCGAT 1 cut(s) 239
BseDI CCNNGG 7 cut(s) 51, 199, 396, 397, 495, 733, 886
BseGI GGATG 3 cut(s) 20, 870, 1477
BseJI GATNNNNATC 3 cut(s) 19, 241, 582
BseLI CCNNNNNNNGG 3 cut(s) 51, 52, 519
BseMI GCAATG 1 cut(s) 1576
BseMII CTCAG 3 cut(s) 563, 1482, 1575
BseNI ACTGG 2 cut(s) 375, 810
BseRI GAGGAG 1 cut(s) 1011
BseXI GCAGC 4 cut(s) 74, 374, 377, 1304
BsgI GTGCAG 2 cut(s) 1249, 1311
BshFI GGCC 3 cut(s) 167, 512, 1545
BshVI ATCGAT 1 cut(s) 239
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 685
BsiWI CGTACG 1 cut(s) 1192
BslFI GGGAC 1 cut(s) 1428
BslI CCNNNNNNNGG 3 cut(s) 51, 52, 519
BsmFI GGGAC 1 cut(s) 1428
BsmI GAATGC 4 cut(s) 272, 360, 773, 1111
BsnI GGCC 3 cut(s) 167, 512, 1545
Bsp1286I GDGCHC 2 cut(s) 108, 432
Bsp1407I TGTACA 1 cut(s) 638
Bsp143I GATC 7 cut(s) 14, 236, 382, 577, 800, 1274, 1525
Bsp19I CCATGG 2 cut(s) 495, 886
BspACI CCGC 2 cut(s) 153, 1208
BspANI GGCC 3 cut(s) 167, 512, 1545
BspCNI CTCAG 3 cut(s) 562, 1483, 1576
BspDI ATCGAT 1 cut(s) 239
BspHI TCATGA 3 cut(s) 991, 1413, 1501
BspLI GGNNCC 2 cut(s) 652, 949
BspMAI CTGCAG 2 cut(s) 13, 293
BspMI ACCTGC 1 cut(s) 886
BspPI GGATC 5 cut(s) 22, 390, 572, 1282, 1533
BspQI GCTCTTC 2 cut(s) 624, 1299
BsrDI GCAATG 1 cut(s) 1576
BsrGI TGTACA 1 cut(s) 638
BsrI ACTGG 2 cut(s) 375, 810
BssECI CCNNGG 7 cut(s) 51, 199, 396, 397, 495, 733, 886
BssMI GATC 7 cut(s) 14, 236, 382, 577, 800, 1274, 1525
BssT1I CCWWGG 2 cut(s) 495, 886
Bst2UI CCWGG 3 cut(s) 52, 200, 398
Bst4CI ACNGT 9 cut(s) 226, 389, 556, 666, 737, 1043, 1068, 1216, 1376
Bst6I CTCTTC 6 cut(s) 624, 640, 1248, 1299, 1343, 1438
BstAUI TGTACA 1 cut(s) 638
BstC8I GCNNGC 4 cut(s) 360, 1338, 1543, 1567
BstDEI CTNAG 3 cut(s) 549, 1491, 1584
BstDSI CCRYGG 3 cut(s) 495, 733, 886
BstF5I GGATG 3 cut(s) 20, 870, 1477
BstHHI GCGC 1 cut(s) 732
BstKTI GATC 7 cut(s) 17, 239, 385, 580, 803, 1277, 1528
BstMBI GATC 7 cut(s) 14, 236, 382, 577, 800, 1274, 1525
BstMWI GCNNNNNNNGC 6 cut(s) 17, 62, 436, 977, 1306, 1571
BstNI CCWGG 3 cut(s) 52, 200, 398
BstSCI CCNGG 3 cut(s) 50, 198, 396
BstSFI CTRYAG 2 cut(s) 9, 289
BstV1I GCAGC 4 cut(s) 74, 374, 377, 1304
Bsu15I ATCGAT 1 cut(s) 239
BsuI GTATCC 1 cut(s) 1146
BsuRI GGCC 3 cut(s) 167, 512, 1545
BsuTUI ATCGAT 1 cut(s) 239
BtgI CCRYGG 3 cut(s) 495, 733, 886
BtrI CACGTC 1 cut(s) 1345
BtsCI GGATG 3 cut(s) 20, 870, 1477
BtsI GCAGTG 3 cut(s) 654, 1302, 1337
BveI ACCTGC 1 cut(s) 886
Cac8I GCNNGC 4 cut(s) 360, 1338, 1543, 1567
CciI TCATGA 3 cut(s) 991, 1413, 1501
CfoI GCGC 1 cut(s) 732
Cfr13I GGNCC 1 cut(s) 510
ClaI ATCGAT 1 cut(s) 239
CseI GACGC 1 cut(s) 1563
Csp6I GTAC 3 cut(s) 639, 1193, 1589
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviAII CATG 9 cut(s) 280, 496, 534, 887, 976, 992, 1004, 1414, 1502
CviQI GTAC 3 cut(s) 639, 1193, 1589
DdeI CTNAG 3 cut(s) 549, 1491, 1584
DpnI GATC 7 cut(s) 16, 238, 384, 579, 802, 1276, 1527
DpnII GATC 7 cut(s) 14, 236, 382, 577, 800, 1274, 1525
EaeI YGGCCR 1 cut(s) 1543
Eam1104I CTCTTC 6 cut(s) 624, 640, 1248, 1299, 1343, 1438
EarI CTCTTC 6 cut(s) 624, 640, 1248, 1299, 1343, 1438
Eco130I CCWWGG 2 cut(s) 495, 886
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 1 cut(s) 243
EcoRI GAATTC 1 cut(s) 1417
EcoRII CCWGG 3 cut(s) 50, 198, 396
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 2 cut(s) 495, 886
EcoT22I ATGCAT 2 cut(s) 257, 775
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 2 cut(s) 495, 886
FaeI CATG 9 cut(s) 283, 499, 537, 890, 979, 995, 1007, 1417, 1505
FaqI GGGAC 1 cut(s) 1428
FatI CATG 9 cut(s) 279, 495, 533, 886, 975, 991, 1003, 1413, 1501
FauNDI CATATG 1 cut(s) 627
Fnu4HI GCNGC 4 cut(s) 63, 363, 366, 1293
FokI GGATG 3 cut(s) 7, 857, 1464
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 4 cut(s) 63, 363, 366, 1293
FspBI CTAG 4 cut(s) 938, 1259, 1407, 1482
GlaI GCGC 1 cut(s) 731
GluI GCNGC 4 cut(s) 63, 363, 366, 1293
HaeIII GGCC 3 cut(s) 167, 512, 1545
HapII CCGG 1 cut(s) 685
HgaI GACGC 1 cut(s) 1563
HhaI GCGC 1 cut(s) 732
Hin1II CATG 9 cut(s) 283, 499, 537, 890, 979, 995, 1007, 1417, 1505
Hin6I GCGC 1 cut(s) 730
HinP1I GCGC 1 cut(s) 730
HincII GTYRAC 2 cut(s) 715, 1061
HindII GTYRAC 2 cut(s) 715, 1061
HinfI GANTC 7 cut(s) 112, 392, 698, 834, 1078, 1410, 1474
HpaII CCGG 1 cut(s) 685
HphI GGTGA 3 cut(s) 268, 484, 1222
Hpy166II GTNNAC 7 cut(s) 32, 597, 706, 715, 759, 1061, 1379
Hpy188I TCNGA 2 cut(s) 552, 833
Hpy8I GTNNAC 7 cut(s) 32, 597, 706, 715, 759, 1061, 1379
Hpy99I CGWCG 1 cut(s) 1397
HpyAV CCTTC 1 cut(s) 501
HpyCH4III ACNGT 9 cut(s) 226, 389, 556, 666, 737, 1043, 1068, 1216, 1376
HpyCH4IV ACGT 1 cut(s) 1344
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 62, 436, 977, 1306, 1571
HpyF3I CTNAG 3 cut(s) 549, 1491, 1584
HpySE526I ACGT 1 cut(s) 1344
Hsp92II CATG 9 cut(s) 283, 499, 537, 890, 979, 995, 1007, 1417, 1505
HspAI GCGC 1 cut(s) 730
Kzo9I GATC 7 cut(s) 14, 236, 382, 577, 800, 1274, 1525
LguI GCTCTTC 2 cut(s) 624, 1299
Lsp1109I GCAGC 4 cut(s) 74, 374, 377, 1304
LweI GCATC 3 cut(s) 29, 1009, 1131
MaeI CTAG 4 cut(s) 938, 1259, 1407, 1482
MaeII ACGT 1 cut(s) 1344
MaeIII GTNAC 8 cut(s) 210, 568, 1043, 1135, 1210, 1232, 1261, 1388
MalI GATC 7 cut(s) 16, 238, 384, 579, 802, 1276, 1527
MboI GATC 7 cut(s) 14, 236, 382, 577, 800, 1274, 1525
MboII GAAGA 9 cut(s) 445, 611, 657, 673, 859, 1235, 1316, 1360, 1425
MhlI GDGCHC 2 cut(s) 108, 432
MlsI TGGCCA 1 cut(s) 1545
MluNI TGGCCA 1 cut(s) 1545
MlyI GAGTC 2 cut(s) 1072, 1483
MmeI TCCRAC 1 cut(s) 811
Mox20I TGGCCA 1 cut(s) 1545
Mph1103I ATGCAT 2 cut(s) 257, 775
MscI TGGCCA 1 cut(s) 1545
MseI TTAA 2 cut(s) 1011, 1451
Msp20I TGGCCA 1 cut(s) 1545
MspI CCGG 1 cut(s) 685
MspR9I CCNGG 3 cut(s) 52, 200, 398
Mva1269I GAATGC 4 cut(s) 272, 360, 773, 1111
MvaI CCWGG 3 cut(s) 52, 200, 398
MwoI GCNNNNNNNGC 6 cut(s) 17, 62, 436, 977, 1306, 1571
NcoI CCATGG 2 cut(s) 495, 886
NdeI CATATG 1 cut(s) 627
NdeII GATC 7 cut(s) 14, 236, 382, 577, 800, 1274, 1525
NlaIII CATG 9 cut(s) 283, 499, 537, 890, 979, 995, 1007, 1417, 1505
NlaIV GGNNCC 2 cut(s) 652, 949
NmuCI GTSAC 7 cut(s) 210, 568, 1043, 1135, 1210, 1261, 1388
NsiI ATGCAT 2 cut(s) 257, 775
PagI TCATGA 3 cut(s) 991, 1413, 1501
PaqCI CACCTGC 1 cut(s) 886
PasI CCCWGGG 1 cut(s) 397
PciSI GCTCTTC 2 cut(s) 624, 1299
PctI GAATGC 4 cut(s) 272, 360, 773, 1111
PfeI GAWTC 5 cut(s) 112, 392, 698, 834, 1410
Pfl23II CGTACG 1 cut(s) 1192
PkrI GCNGC 4 cut(s) 64, 364, 367, 1294
PleI GAGTC 2 cut(s) 1072, 1482
PpsI GAGTC 2 cut(s) 1072, 1482
PsiI TTATAA 2 cut(s) 1187, 1325
Psp6I CCWGG 3 cut(s) 50, 198, 396
PspGI CCWGG 3 cut(s) 50, 198, 396
PspLI CGTACG 1 cut(s) 1192
PspN4I GGNNCC 2 cut(s) 652, 949
PspPI GGNCC 1 cut(s) 510
PstI CTGCAG 2 cut(s) 13, 293
RsaI GTAC 3 cut(s) 640, 1194, 1590
RsaNI GTAC 3 cut(s) 639, 1193, 1589
SapI GCTCTTC 2 cut(s) 624, 1299
SaqAI TTAA 2 cut(s) 1011, 1451
SatI GCNGC 4 cut(s) 63, 363, 366, 1293
Sau3AI GATC 7 cut(s) 14, 236, 382, 577, 800, 1274, 1525
Sau96I GGNCC 1 cut(s) 510
SchI GAGTC 2 cut(s) 1072, 1483
ScrFI CCNGG 3 cut(s) 52, 200, 398
SduI GDGCHC 2 cut(s) 108, 432
SfaNI GCATC 3 cut(s) 29, 1009, 1131
SfcI CTRYAG 2 cut(s) 9, 289
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
SsiI CCGC 2 cut(s) 153, 1208
SspMI CTAG 4 cut(s) 938, 1259, 1407, 1482
StyD4I CCNGG 3 cut(s) 50, 198, 396
StyI CCWWGG 2 cut(s) 495, 886
TaaI ACNGT 9 cut(s) 226, 389, 556, 666, 737, 1043, 1068, 1216, 1376
TaiI ACGT 1 cut(s) 1347
TaqI TCGA 4 cut(s) 162, 239, 622, 777
TatI WGTACW 1 cut(s) 638
TfiI GAWTC 5 cut(s) 112, 392, 698, 834, 1410
Tru1I TTAA 2 cut(s) 1011, 1451
Tru9I TTAA 2 cut(s) 1011, 1451
TseFI GTSAC 7 cut(s) 210, 568, 1043, 1135, 1210, 1261, 1388
TseI GCWGC 4 cut(s) 62, 362, 365, 1292
Tsp45I GTSAC 7 cut(s) 210, 568, 1043, 1135, 1210, 1261, 1388
XapI RAATTY 4 cut(s) 1279, 1399, 1417, 1495
XbaI TCTAGA 1 cut(s) 1406
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XspI CTAG 4 cut(s) 938, 1259, 1407, 1482
Zsp2I ATGCAT 2 cut(s) 257, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.