pycom12g08900

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003273
Physical Location & Seq
Reverse (-)
10594006 .. 10595942
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 984 bp
ATGAATTATTTTTTCTTTATTGACATCCGAGTGCCGCGTTCATACGCGGGCGAGAAACACAGAAACAGAAGCCTCACCGGAAAGGGAAGAGGTTTACTGCAATTACCACTTATCTCTGATCCGCTCTTTGATACAAACATAAACATGAAGAAGTCGCATCAAAGCCAACCCATATACATTTTCTGTTTAGTATTCTTTTTATGTTCCGCAACATCGATCACAGCTCAAGAAGTTGAAGATGAACGAGAGTTCGATTATCTTCAGGGAAGTGGAAAGGGACCGAAGCAGTGGGGAGCGATGAAGAAAGAATGGTCAGCCTGCAAGAATGGAGGCATGCAATCTCCTATTGATTTATCGAGTCACAGGGTCACGTTAGTCCCAAACTTGGGCAAGCTCAACATCTCTTACAATCCTTGTAATGCCACCGTAAAGAATAGAGGCCACGATATTTCGATTTACTGGGAGGGCAACGCTGGATCAATTCACATAAATGGTACTCAGTATTTGCTAAAACAATCTCACTGGCACTCCCCTTCCGAGCATTCCGTCAATGGCAGGAGGTACGACATGGAGCTCCACATGGTTCATCTAAGCTCCGATCCCAAAGTCACAAACAAGATCGCTGTTGTTGGCGTCCTATACAAGATTGGTCGCCCTGACTCTTTCCTTTCTAAGTTGACAGGGCATGTAAAGTCAATGATGGATCAGATGGAAGAGAGAAACACAGGGACAATTGATCCCCGAGAAATTAAAATCCATAAGAAAAACTATTACAGATACATGGGCTCACTCACTGTTCCTCCTTGCACTGAAGGCGTTATTTGGACCATCAATAAAAAGATAAGAACAGTCTCAAGGGATCAAGTAAAGCTATTTCGAGTCGCTGTGCATGATTATGCAGAGATGAATGCAAGGCCAGTACAACCACTTAATCTTCGAGAGATCCAGGTCTTTGACAGAAGAAAAGGGAGCACAAATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

328

Amino Acids

37.42

Weight (kDa)

9.57

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 93 - 316 3.6e-45 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 124
AccII CGCG 2 cut(s) 37, 47
AciI CCGC 4 cut(s) 35, 47, 122, 207
AclWI GGATC 7 cut(s) 113, 484, 593, 711, 731, 867, 937
AcuI CTGAAG 2 cut(s) 245, 831
AcyI GRCGYC 1 cut(s) 633
AfaI GTAC 3 cut(s) 496, 563, 921
AfiI CCNNNNNNNGG 2 cut(s) 385, 386
AgsI TTSAA 1 cut(s) 236
AjnI CCWGG 1 cut(s) 945
AluBI AGCT 5 cut(s) 224, 394, 574, 594, 871
AluI AGCT 5 cut(s) 224, 394, 574, 594, 871
Alw21I GWGCWC 2 cut(s) 576, 974
Alw26I GTCTC 1 cut(s) 856
AlwI GGATC 7 cut(s) 113, 484, 593, 711, 731, 867, 937
Ama87I CYCGRG 1 cut(s) 741
AoxI GGCC 2 cut(s) 439, 914
AspS9I GGNCC 2 cut(s) 278, 825
AsuHPI GGTGA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 741
AvaII GGWCC 2 cut(s) 278, 825
BanII GRGCYC 2 cut(s) 576, 788
Bbv12I GWGCWC 2 cut(s) 576, 974
BccI CCATC 3 cut(s) 694, 703, 836
BciT130I CCWGG 1 cut(s) 947
BcoDI GTCTC 1 cut(s) 856
BisI GCNGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 947
Bme18I GGWCC 2 cut(s) 278, 825
BmeT110I CYCGRG 1 cut(s) 741
BmgT120I GGNCC 2 cut(s) 278, 825
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 947
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 1 cut(s) 166
BmuI ACTGGG 1 cut(s) 469
BpuEI CTTGAG 2 cut(s) 210, 838
Bsa29I ATCGAT 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 633
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 4 cut(s) 512, 542, 784, 814
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 386
Bse1I ACTGG 3 cut(s) 464, 527, 917
BseBI CCWGG 1 cut(s) 947
BseCI ATCGAT 1 cut(s) 215
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 2 cut(s) 385, 386
BseMII CTCAG 1 cut(s) 512
BseNI ACTGG 3 cut(s) 464, 527, 917
Bsh1236I CGCG 2 cut(s) 37, 47
BshFI GGCC 2 cut(s) 441, 916
BshVI ATCGAT 1 cut(s) 215
BsiHKAI GWGCWC 2 cut(s) 576, 974
BsiHKCI CYCGRG 1 cut(s) 741
BsiSI CCGG 1 cut(s) 78
BslFI GGGAC 3 cut(s) 291, 362, 742
BslI CCNNNNNNNGG 2 cut(s) 385, 386
BsmAI GTCTC 1 cut(s) 856
BsmFI GGGAC 3 cut(s) 291, 362, 742
BsmI GAATGC 2 cut(s) 541, 913
BsnI GGCC 2 cut(s) 441, 916
BsoBI CYCGRG 1 cut(s) 741
Bsp1286I GDGCHC 3 cut(s) 576, 788, 974
Bsp143I GATC 9 cut(s) 118, 216, 476, 598, 618, 703, 736, 859, 942
BspACI CCGC 4 cut(s) 35, 47, 122, 207
BspANI GGCC 2 cut(s) 441, 916
BspCNI CTCAG 1 cut(s) 511
BspDI ATCGAT 1 cut(s) 215
BspFNI CGCG 2 cut(s) 37, 47
BspLI GGNNCC 1 cut(s) 279
BspPI GGATC 7 cut(s) 113, 484, 593, 711, 731, 867, 937
BsrBI CCGCTC 1 cut(s) 124
BsrI ACTGG 3 cut(s) 464, 527, 917
BssMI GATC 9 cut(s) 118, 216, 476, 598, 618, 703, 736, 859, 942
BssNI GRCGYC 1 cut(s) 633
Bst2UI CCWGG 1 cut(s) 947
Bst4CI ACNGT 3 cut(s) 427, 796, 850
Bst6I CTCTTC 2 cut(s) 82, 708
BstACI GRCGYC 1 cut(s) 633
BstC8I GCNNGC 4 cut(s) 49, 319, 335, 392
BstDEI CTNAG 3 cut(s) 498, 590, 672
BstF5I GGATG 1 cut(s) 24
BstFNI CGCG 2 cut(s) 37, 47
BstKTI GATC 9 cut(s) 121, 219, 479, 601, 621, 706, 739, 862, 945
BstMAI GTCTC 1 cut(s) 856
BstMBI GATC 9 cut(s) 118, 216, 476, 598, 618, 703, 736, 859, 942
BstMWI GCNNNNNNNGC 1 cut(s) 813
BstNI CCWGG 1 cut(s) 947
BstNSI RCATGY 2 cut(s) 337, 689
BstSCI CCNGG 1 cut(s) 945
BstUI CGCG 2 cut(s) 37, 47
BstX2I RGATCY 1 cut(s) 942
BstYI RGATCY 1 cut(s) 942
Bsu15I ATCGAT 1 cut(s) 215
BsuRI GGCC 2 cut(s) 441, 916
BsuTUI ATCGAT 1 cut(s) 215
BtgZI GCGATG 1 cut(s) 311
BtsCI GGATG 1 cut(s) 24
BtsI GCAGTG 1 cut(s) 293
BtsIMutI CAGTG 4 cut(s) 293, 520, 792, 807
Cac8I GCNNGC 4 cut(s) 49, 319, 335, 392
Cfr13I GGNCC 2 cut(s) 278, 825
ClaI ATCGAT 1 cut(s) 215
CseI GACGC 1 cut(s) 622
Csp6I GTAC 3 cut(s) 495, 562, 920
CviAII CATG 7 cut(s) 145, 334, 568, 580, 686, 781, 890
CviQI GTAC 3 cut(s) 495, 562, 920
DdeI CTNAG 3 cut(s) 498, 590, 672
DpnI GATC 9 cut(s) 120, 218, 478, 600, 620, 705, 738, 861, 944
DpnII GATC 9 cut(s) 118, 216, 476, 598, 618, 703, 736, 859, 942
Eam1104I CTCTTC 2 cut(s) 82, 708
EarI CTCTTC 2 cut(s) 82, 708
Ecl136II GAGCTC 1 cut(s) 574
Eco24I GRGCYC 2 cut(s) 576, 788
Eco47I GGWCC 2 cut(s) 278, 825
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 2 cut(s) 245, 831
Eco88I CYCGRG 1 cut(s) 741
EcoICRI GAGCTC 1 cut(s) 574
EcoRII CCWGG 1 cut(s) 945
EcoT38I GRGCYC 2 cut(s) 576, 788
FaeI CATG 7 cut(s) 148, 337, 571, 583, 689, 784, 893
FaqI GGGAC 3 cut(s) 291, 362, 742
FatI CATG 7 cut(s) 144, 333, 567, 579, 685, 780, 889
FauI CCCGC 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 35
FokI GGATG 1 cut(s) 11
FriOI GRGCYC 2 cut(s) 576, 788
Fsp4HI GCNGC 1 cut(s) 35
GluI GCNGC 1 cut(s) 35
HaeIII GGCC 2 cut(s) 441, 916
HapII CCGG 1 cut(s) 78
HgaI GACGC 1 cut(s) 622
Hin1I GRCGYC 1 cut(s) 633
Hin1II CATG 7 cut(s) 148, 337, 571, 583, 689, 784, 893
HincII GTYRAC 1 cut(s) 678
HindII GTYRAC 1 cut(s) 678
HinfI GANTC 3 cut(s) 358, 659, 879
HpaII CCGG 1 cut(s) 78
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 2 cut(s) 95, 678
Hpy188I TCNGA 5 cut(s) 29, 118, 538, 598, 708
Hpy188III TCNNGA 2 cut(s) 227, 938
Hpy8I GTNNAC 2 cut(s) 95, 678
HpyAV CCTTC 2 cut(s) 543, 806
HpyCH4III ACNGT 3 cut(s) 427, 796, 850
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 7 cut(s) 100, 321, 337, 807, 889, 899, 911
HpyF10VI GCNNNNNNNGC 1 cut(s) 813
HpyF3I CTNAG 3 cut(s) 498, 590, 672
HpySE526I ACGT 1 cut(s) 371
Hsp92I GRCGYC 1 cut(s) 633
Hsp92II CATG 7 cut(s) 148, 337, 571, 583, 689, 784, 893
Kzo9I GATC 9 cut(s) 118, 216, 476, 598, 618, 703, 736, 859, 942
LmnI GCTCC 5 cut(s) 293, 571, 579, 599, 969
LweI GCATC 1 cut(s) 166
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 3 cut(s) 359, 367, 607
MalI GATC 9 cut(s) 120, 218, 478, 600, 620, 705, 738, 861, 944
MbiI CCGCTC 1 cut(s) 124
MboI GATC 9 cut(s) 118, 216, 476, 598, 618, 703, 736, 859, 942
MboII GAAGA 8 cut(s) 99, 160, 248, 251, 313, 725, 926, 972
MfeI CAATTG 1 cut(s) 732
MflI RGATCY 1 cut(s) 942
MhlI GDGCHC 3 cut(s) 576, 788, 974
MluCI AATT 5 cut(s) 4, 101, 480, 732, 747
MlyI GAGTC 3 cut(s) 367, 653, 888
MnlI CCTC 7 cut(s) 83, 83, 323, 431, 457, 552, 810
MseI TTAA 2 cut(s) 750, 930
MslI CAYNNNNRTG 4 cut(s) 29, 143, 489, 894
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 1 cut(s) 947
MunI CAATTG 1 cut(s) 732
Mva1269I GAATGC 2 cut(s) 541, 913
MvaI CCWGG 1 cut(s) 947
MvnI CGCG 2 cut(s) 37, 47
MwoI GCNNNNNNNGC 1 cut(s) 813
NdeII GATC 9 cut(s) 118, 216, 476, 598, 618, 703, 736, 859, 942
NlaIII CATG 7 cut(s) 148, 337, 571, 583, 689, 784, 893
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 3 cut(s) 359, 367, 607
NspI RCATGY 2 cut(s) 337, 689
PaeI GCATGC 1 cut(s) 337
PctI GAATGC 2 cut(s) 541, 913
PkrI GCNGC 1 cut(s) 36
PleI GAGTC 3 cut(s) 366, 653, 887
PpsI GAGTC 3 cut(s) 366, 653, 887
Psp124BI GAGCTC 1 cut(s) 576
Psp6I CCWGG 1 cut(s) 945
PspGI CCWGG 1 cut(s) 945
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 2 cut(s) 278, 825
PsuI RGATCY 1 cut(s) 942
RsaI GTAC 3 cut(s) 496, 563, 921
RsaNI GTAC 3 cut(s) 495, 562, 920
RseI CAYNNNNRTG 4 cut(s) 29, 143, 489, 894
SacI GAGCTC 1 cut(s) 576
SaqAI TTAA 2 cut(s) 750, 930
SatI GCNGC 1 cut(s) 35
Sau3AI GATC 9 cut(s) 118, 216, 476, 598, 618, 703, 736, 859, 942
Sau96I GGNCC 2 cut(s) 278, 825
SchI GAGTC 3 cut(s) 367, 653, 888
ScrFI CCNGG 1 cut(s) 947
SduI GDGCHC 3 cut(s) 576, 788, 974
SetI ASST 9 cut(s) 94, 226, 374, 396, 563, 576, 596, 873, 951
SfaNI GCATC 1 cut(s) 166
SinI GGWCC 2 cut(s) 278, 825
SmiMI CAYNNNNRTG 4 cut(s) 29, 143, 489, 894
SmlI CTYRAG 2 cut(s) 225, 853
SmoI CTYRAG 2 cut(s) 225, 853
SphI GCATGC 1 cut(s) 337
Sse9I AATT 5 cut(s) 4, 101, 480, 732, 747
SsiI CCGC 4 cut(s) 35, 47, 122, 207
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 1 cut(s) 945
TaaI ACNGT 3 cut(s) 427, 796, 850
TaiI ACGT 1 cut(s) 374
TaqI TCGA 6 cut(s) 215, 252, 356, 452, 877, 937
TaqII GACCGA 1 cut(s) 295
TasI AATT 5 cut(s) 4, 101, 480, 732, 747
TatI WGTACW 1 cut(s) 919
TauI GCSGC 1 cut(s) 37
Tru1I TTAA 2 cut(s) 750, 930
Tru9I TTAA 2 cut(s) 750, 930
TscAI CASTG 4 cut(s) 293, 527, 799, 814
TseFI GTSAC 3 cut(s) 359, 367, 607
Tsp45I GTSAC 3 cut(s) 359, 367, 607
TspDTI ATGAA 7 cut(s) 17, 30, 161, 255, 314, 575, 920
TspGWI ACGGA 1 cut(s) 535
TspRI CASTG 4 cut(s) 293, 527, 799, 814
VpaK11BI GGWCC 2 cut(s) 278, 825
XceI RCATGY 2 cut(s) 337, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.