pycom12g12040

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002520
Physical Location & Seq
Forward (+)
14497097 .. 14501676
4580 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1854 bp
ATGGTGGTCATGGGAAAGGAATGTGATGATGACAATTGCATGTGGAAAGAAAACATTTTCCAATTCTCAAAACTTCGTTCCAAAAAGTCTGCGTTCTTCCCGTCACATTTTTTGAAGAAAATAGGACACAAGAAGAACAAATATCACATAAAAGACCAAAACGGAGAAGAAGAAGAAGAGATATTCAAGGCAGTCATGGCAGGAGACTCCAAAGGCGCCTCTCTGGAATACACTCCGACATGGGCACTCGCCACCATTTGCTTCGTCTTCATCTTCACCTCCCTTTTCATCGAGCACTCCATCCATCTCCTTGTCAACGACACATCTAATTTGGTGCAGTTTCTCAAAAGGCACAGGAAAACCGCCTTGATGGACGCGGTTGAGAAGCTCAAATCAGAGTTGATGCTTATGGGATTTGTGTCACTTTTGCTGGCAATAACCCAAAATTCGATATCTAAAATCTGTATATCTGCAAAGGTGGGAAACATCATGCTTCCATGTCGCAGAAATGAGACAACCAAAAAAGAAGATGTCGAGAAATTTGTGATTGTGAATAAGATTGTGACAAACTTTTCGACCGGCGCAAATAGTTTGTATGATGAAATACTAAGGCAAATATCAGTGCATAGGCGACTTGCAGAGGAGGATGCAGATGCTACAGGTTCTTGCGGTGAGGGAAAATTACCTTTCATGACGAAAGATGCTCTTCATCAGCTTCACTTACTTATTTTTGCGCTGGCGGTTATGCATATTGTGTATAGTGTTCTTACCATGGCTTTGGGGAGGGCCAAGATGACGCGCTGGGAAGCTTGGGAGCAAGAAACTCGAACAATGGAGTACCAAGTTGAGAATGATCCCAACCGGTTCAGGATTACCAGGCAAACAACATTTGGAAGAAGGCACATGAATTCTAGTTCAGAAACGTCATTTCATCTGTGGATGAGATGTTTTTTGAGGCAATTCCATAACTCAGTGGCAAAAGTTGACTACATCACCATGAGACATGGCTTCATAGCGGCTCATTTGCCAAGCAGGCAAAGCTTTGATTTCCAAAAATACATACAGCGATCTTTGGAGGAAGATTTCAAGATCATGGTTGCAATCAGCCCCTTAATGTGGTTTGCAGTAGCAATTCTCATGCTCCTCGACGTGTATGGTTGGTATATATATCTCTGGCTTTCCTATGTTCCACTCTTGGTCGTTCTGGTTCTAGGAACCAAACTTCAGTATATCGTTGCAAAAATGGCTCTTCAAATTAAAGACCAGAACAGTGTGATCGTCGGAACACCACTTGTGCGAGTAAACGACGATCTCTTCTGGTTCAGGAAACCTAGATTTGTCTTGGTGCTGCTACATTATACTCTATTTTTGAATGCATTTGAGCTGGCCTTCTTCATTTGGGTTACAATCCAATTCGGGTTCACATCGTGCTACCACGAGCACACTGTGGTCATAATTACGAGGATTTCCTTAGCGGTGGCTACTCAAGTCCTCTGCAGCTACATCACTCTTCCTCTCTATGCACTTGTCATACAGATGGGTTCACAGTTCAAGGGTAAGATTATGGAAGACAACATGGCAGACATTTTAAAGCAATGGCATGCTGAGGTGAGGAATAGAAGGAGAAGGGAACAACAAACTCCGCAATCAGGAAGAACATCTTTCTCCACAGATTGGAGCTCCGTGAGGAATAGCCTTTCAGAAATGCCTTCTTATATAAGAAGGGTTTTCAAACCAGATGAACCAGCTGAAAGCAGTGTCCAGTTGTCTAACAGAGGGAATGTACAAGACGAGATAGTAGAACAAGAGGGAAGCTCAAGGGGTTATAGAAGAAATGGGGATTATGGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

618

Amino Acids

71.48

Weight (kDa)

9.08

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 73 - 559 3.9e-186 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 215
AccB7I CCANNNNNTGG 1 cut(s) 1674
AccII CGCG 2 cut(s) 377, 799
AciI CCGC 7 cut(s) 363, 377, 669, 740, 1016, 1475, 1643
AclWI GGATC 1 cut(s) 848
AcsI RAATTY 3 cut(s) 445, 539, 907
AcuI CTGAAG 1 cut(s) 1208
AcyI GRCGYC 1 cut(s) 216
AdeI CACNNNGTG 2 cut(s) 1428, 1447
AfaI GTAC 2 cut(s) 839, 1785
AfiI CCNNNNNNNGG 3 cut(s) 1116, 1649, 1674
AflIII ACRYGT 1 cut(s) 1149
AgeI ACCGGT 1 cut(s) 861
AgsI TTSAA 7 cut(s) 115, 187, 1087, 1253, 1372, 1552, 1732
AjiI CACGTC 1 cut(s) 1150
AjnI CCWGG 1 cut(s) 875
AluBI AGCT 9 cut(s) 388, 715, 809, 1041, 1384, 1500, 1680, 1748, 1815
AluI AGCT 9 cut(s) 388, 715, 809, 1041, 1384, 1500, 1680, 1748, 1815
Alw21I GWGCWC 3 cut(s) 297, 1443, 1682
Alw26I GTCTC 3 cut(s) 198, 506, 994
AlwI GGATC 1 cut(s) 848
AoxI GGCC 2 cut(s) 786, 1386
ApeKI GCWGC 2 cut(s) 1348, 1497
ApoI RAATTY 3 cut(s) 445, 539, 907
ArsI GACNNNNNNTTYG 2 cut(s) 556, 588
AsiGI ACCGGT 1 cut(s) 861
AspLEI GCGC 4 cut(s) 218, 584, 736, 801
AspS9I GGNCC 1 cut(s) 786
AsuHPI GGTGA 4 cut(s) 268, 683, 985, 1621
BaeGI GKGCMC 1 cut(s) 247
BanI GGYRCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 1682
BauI CACGAG 1 cut(s) 1436
BbsI GAAGAC 2 cut(s) 259, 1575
Bbv12I GWGCWC 3 cut(s) 297, 1443, 1682
BbvCI CCTCAGC 1 cut(s) 1605
BbvI GCAGC 2 cut(s) 1335, 1509
BccI CCATC 4 cut(s) 308, 312, 364, 1531
BcgI CGANNNNNNTGC 2 cut(s) 806, 840
BciT130I CCWGG 1 cut(s) 877
BcoDI GTCTC 3 cut(s) 198, 506, 994
BfaI CTAG 3 cut(s) 912, 1211, 1332
BfmI CTRYAG 2 cut(s) 657, 1495
BfoI RGCGCY 1 cut(s) 219
BglI GCCNNNNNGGC 1 cut(s) 1033
BisI GCNGC 3 cut(s) 1017, 1349, 1498
BlsI GCNGC 3 cut(s) 1018, 1350, 1499
Bme1390I CCNGG 1 cut(s) 877
BmgBI CACGTC 1 cut(s) 1150
BmgT120I GGNCC 1 cut(s) 786
BmiI GGNNCC 2 cut(s) 217, 1216
BmrFI CCNGG 1 cut(s) 877
BmsI GCATC 4 cut(s) 393, 637, 643, 691
BpiI GAAGAC 2 cut(s) 259, 1575
BplI GAGNNNNNCTC 2 cut(s) 1799, 1831
Bpu10I CCTNAGC 2 cut(s) 1471, 1605
BpuEI CTTGAG 2 cut(s) 1470, 1801
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 1 cut(s) 771
BsaWI WCCGGW 1 cut(s) 861
Bsc4I CCNNNNNNNGG 3 cut(s) 1116, 1649, 1674
Bse118I RCCGGY 2 cut(s) 578, 861
Bse1I ACTGG 1 cut(s) 1762
Bse3DI GCAATG 1 cut(s) 1601
BseBI CCWGG 1 cut(s) 877
BseDI CCNNGG 1 cut(s) 771
BseGI GGATG 3 cut(s) 300, 652, 945
BseLI CCNNNNNNNGG 3 cut(s) 1116, 1649, 1674
BseMI GCAATG 1 cut(s) 1601
BseMII CTCAG 2 cut(s) 984, 1596
BseNI ACTGG 1 cut(s) 1762
BseRI GAGGAG 2 cut(s) 656, 1133
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 2 cut(s) 1335, 1509
BseYI CCCAGC 1 cut(s) 801
BsgI GTGCAG 1 cut(s) 356
Bsh1236I CGCG 2 cut(s) 377, 799
Bsh1285I CGRYCG 1 cut(s) 579
BshFI GGCC 2 cut(s) 788, 1388
BshNI GGYRCC 1 cut(s) 215
BshTI ACCGGT 1 cut(s) 861
BsiEI CGRYCG 1 cut(s) 579
BsiHKAI GWGCWC 3 cut(s) 297, 1443, 1682
BsiSI CCGG 2 cut(s) 579, 862
BslI CCNNNNNNNGG 3 cut(s) 1116, 1649, 1674
BsmAI GTCTC 3 cut(s) 198, 506, 994
BsmI GAATGC 1 cut(s) 1378
BsnI GGCC 2 cut(s) 788, 1388
Bsp1286I GDGCHC 4 cut(s) 247, 297, 1443, 1682
Bsp1407I TGTACA 1 cut(s) 1783
Bsp143I GATC 5 cut(s) 853, 1067, 1089, 1275, 1309
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 7 cut(s) 363, 377, 669, 740, 1016, 1475, 1643
BspANI GGCC 2 cut(s) 788, 1388
BspCNI CTCAG 2 cut(s) 983, 1597
BspFNI CGCG 2 cut(s) 377, 799
BspHI TCATGA 1 cut(s) 690
BspLI GGNNCC 2 cut(s) 217, 1216
BspMAI CTGCAG 1 cut(s) 1499
BspPI GGATC 1 cut(s) 848
BspQI GCTCTTC 2 cut(s) 711, 1254
BspT107I GGYRCC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 1601
BsrFI RCCGGY 2 cut(s) 578, 861
BsrGI TGTACA 1 cut(s) 1783
BsrI ACTGG 1 cut(s) 1762
BssAI RCCGGY 2 cut(s) 578, 861
BssECI CCNNGG 1 cut(s) 771
BssMI GATC 5 cut(s) 853, 1067, 1089, 1275, 1309
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 1436
BssT1I CCWWGG 1 cut(s) 771
Bst2BI CACGAG 1 cut(s) 1436
Bst2UI CCWGG 1 cut(s) 877
Bst4CI ACNGT 3 cut(s) 1271, 1447, 1548
Bst6I CTCTTC 5 cut(s) 171, 711, 1254, 1319, 1515
BstACI GRCGYC 1 cut(s) 216
BstAUI TGTACA 1 cut(s) 1783
BstC8I GCNNGC 5 cut(s) 432, 738, 1034, 1386, 1602
BstDEI CTNAG 4 cut(s) 608, 970, 1471, 1605
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 3 cut(s) 300, 652, 945
BstFNI CGCG 2 cut(s) 377, 799
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 4 cut(s) 218, 584, 736, 801
BstKTI GATC 5 cut(s) 856, 1070, 1092, 1278, 1312
BstMAI GTCTC 3 cut(s) 198, 506, 994
BstMBI GATC 5 cut(s) 853, 1067, 1089, 1275, 1309
BstMCI CGRYCG 1 cut(s) 579
BstMWI GCNNNNNNNGC 4 cut(s) 197, 1033, 1038, 1244
BstNI CCWGG 1 cut(s) 877
BstNSI RCATGY 2 cut(s) 43, 1604
BstSCI CCNGG 1 cut(s) 875
BstSFI CTRYAG 2 cut(s) 657, 1495
BstSLI GKGCMC 1 cut(s) 247
BstUI CGCG 2 cut(s) 377, 799
BstV1I GCAGC 2 cut(s) 1335, 1509
BstV2I GAAGAC 2 cut(s) 259, 1575
BstXI CCANNNNNNTGG 1 cut(s) 778
BsuRI GGCC 2 cut(s) 788, 1388
BtgI CCRYGG 1 cut(s) 771
BtrI CACGTC 1 cut(s) 1150
BtsCI GGATG 3 cut(s) 300, 652, 945
BtsI GCAGTG 1 cut(s) 1762
BtsIMutI CAGTG 5 cut(s) 627, 978, 1276, 1443, 1762
Cac8I GCNNGC 5 cut(s) 432, 738, 1034, 1386, 1602
CciI TCATGA 1 cut(s) 690
CfoI GCGC 4 cut(s) 218, 584, 736, 801
Cfr10I RCCGGY 2 cut(s) 578, 861
Cfr13I GGNCC 1 cut(s) 786
CseI GACGC 2 cut(s) 383, 805
Csp6I GTAC 2 cut(s) 838, 1784
CspAI ACCGGT 1 cut(s) 861
CviQI GTAC 2 cut(s) 838, 1784
DdeI CTNAG 4 cut(s) 608, 970, 1471, 1605
DinI GGCGCC 1 cut(s) 217
DpnI GATC 5 cut(s) 855, 1069, 1091, 1277, 1311
DpnII GATC 5 cut(s) 853, 1067, 1089, 1275, 1309
DraI TTTAAA 1 cut(s) 1590
DraIII CACNNNGTG 2 cut(s) 1428, 1447
Eam1104I CTCTTC 5 cut(s) 171, 711, 1254, 1319, 1515
EarI CTCTTC 5 cut(s) 171, 711, 1254, 1319, 1515
Ecl136II GAGCTC 1 cut(s) 1680
Eco130I CCWWGG 1 cut(s) 771
Eco24I GRGCYC 1 cut(s) 1682
Eco32I GATATC 1 cut(s) 453
Eco53kI GAGCTC 1 cut(s) 1680
Eco57I CTGAAG 1 cut(s) 1208
EcoICRI GAGCTC 1 cut(s) 1680
EcoRI GAATTC 1 cut(s) 907
EcoRII CCWGG 1 cut(s) 875
EcoRV GATATC 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 771
EcoT22I ATGCAT 2 cut(s) 750, 1378
EcoT38I GRGCYC 1 cut(s) 1682
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 771
FalI AAGNNNNNCTT 4 cut(s) 690, 722, 1645, 1677
Fnu4HI GCNGC 3 cut(s) 1017, 1349, 1498
FokI GGATG 3 cut(s) 287, 659, 952
FriOI GRGCYC 1 cut(s) 1682
Fsp4HI GCNGC 3 cut(s) 1017, 1349, 1498
FspBI CTAG 3 cut(s) 912, 1211, 1332
GlaI GCGC 4 cut(s) 217, 583, 735, 800
GluI GCNGC 3 cut(s) 1017, 1349, 1498
GsaI CCCAGC 1 cut(s) 805
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 2 cut(s) 788, 1388
HapII CCGG 2 cut(s) 579, 862
HgaI GACGC 2 cut(s) 383, 805
HhaI GCGC 4 cut(s) 218, 584, 736, 801
Hin1I GRCGYC 1 cut(s) 216
Hin6I GCGC 4 cut(s) 216, 582, 734, 799
HinP1I GCGC 4 cut(s) 216, 582, 734, 799
HincII GTYRAC 2 cut(s) 316, 985
HindII GTYRAC 2 cut(s) 316, 985
HindIII AAGCTT 2 cut(s) 807, 1039
HinfI GANTC 1 cut(s) 206
HpaII CCGG 2 cut(s) 579, 862
HphI GGTGA 4 cut(s) 268, 683, 985, 1621
Hpy166II GTNNAC 5 cut(s) 316, 985, 1303, 1422, 1544
Hpy188I TCNGA 5 cut(s) 237, 397, 919, 1283, 1702
Hpy188III TCNNGA 7 cut(s) 224, 535, 691, 868, 1087, 1324, 1650
Hpy8I GTNNAC 5 cut(s) 316, 985, 1303, 1422, 1544
Hpy99I CGWCG 3 cut(s) 1151, 1283, 1310
HpyAV CCTTC 6 cut(s) 891, 1399, 1614, 1620, 1716, 1719
HpyCH4III ACNGT 3 cut(s) 1271, 1447, 1548
HpyCH4IV ACGT 2 cut(s) 923, 1149
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 1033, 1038, 1244
HpyF3I CTNAG 4 cut(s) 608, 970, 1471, 1605
HpySE526I ACGT 2 cut(s) 923, 1149
Hsp92I GRCGYC 1 cut(s) 216
HspAI GCGC 4 cut(s) 216, 582, 734, 799
KasI GGCGCC 1 cut(s) 215
Kzo9I GATC 5 cut(s) 853, 1067, 1089, 1275, 1309
LguI GCTCTTC 2 cut(s) 711, 1254
LmnI GCTCC 4 cut(s) 814, 1146, 1677, 1685
Lsp1109I GCAGC 2 cut(s) 1335, 1509
LweI GCATC 4 cut(s) 393, 637, 643, 691
MaeI CTAG 3 cut(s) 912, 1211, 1332
MaeII ACGT 2 cut(s) 923, 1149
MaeIII GTNAC 4 cut(s) 102, 420, 562, 1402
MalI GATC 5 cut(s) 855, 1069, 1091, 1277, 1311
MboI GATC 5 cut(s) 853, 1067, 1089, 1275, 1309
MfeI CAATTG 1 cut(s) 34
MhlI GDGCHC 4 cut(s) 247, 297, 1443, 1682
Mly113I GGCGCC 1 cut(s) 216
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 260, 1261
Mph1103I ATGCAT 2 cut(s) 750, 1378
MseI TTAA 3 cut(s) 1112, 1257, 1589
MslI CAYNNNNRTG 2 cut(s) 995, 1535
MspA1I CMGCKG 1 cut(s) 1748
MspI CCGG 2 cut(s) 579, 862
MspR9I CCNGG 1 cut(s) 877
MunI CAATTG 1 cut(s) 34
Mva1269I GAATGC 1 cut(s) 1378
MvaI CCWGG 1 cut(s) 877
MvnI CGCG 2 cut(s) 377, 799
MwoI GCNNNNNNNGC 4 cut(s) 197, 1033, 1038, 1244
NarI GGCGCC 1 cut(s) 216
NcoI CCATGG 1 cut(s) 771
NdeII GATC 5 cut(s) 853, 1067, 1089, 1275, 1309
NlaIV GGNNCC 2 cut(s) 217, 1216
NmuCI GTSAC 3 cut(s) 102, 420, 562
NsiI ATGCAT 2 cut(s) 750, 1378
NspI RCATGY 2 cut(s) 43, 1604
PaeI GCATGC 1 cut(s) 1604
PagI TCATGA 1 cut(s) 690
PciSI GCTCTTC 2 cut(s) 711, 1254
PctI GAATGC 1 cut(s) 1378
PflMI CCANNNNNTGG 1 cut(s) 1674
PinAI ACCGGT 1 cut(s) 861
PkrI GCNGC 3 cut(s) 1018, 1350, 1499
PleI GAGTC 1 cut(s) 200
PluTI GGCGCC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 200
Psp124BI GAGCTC 1 cut(s) 1682
Psp6I CCWGG 1 cut(s) 875
PspFI CCCAGC 1 cut(s) 801
PspGI CCWGG 1 cut(s) 875
PspN4I GGNNCC 2 cut(s) 217, 1216
PspPI GGNCC 1 cut(s) 786
PstI CTGCAG 1 cut(s) 1499
PvuII CAGCTG 1 cut(s) 1748
RsaI GTAC 2 cut(s) 839, 1785
RsaNI GTAC 2 cut(s) 838, 1784
RseI CAYNNNNRTG 2 cut(s) 995, 1535
SacI GAGCTC 1 cut(s) 1682
SapI GCTCTTC 2 cut(s) 711, 1254
SaqAI TTAA 3 cut(s) 1112, 1257, 1589
SatI GCNGC 3 cut(s) 1017, 1349, 1498
Sau3AI GATC 5 cut(s) 853, 1067, 1089, 1275, 1309
Sau96I GGNCC 1 cut(s) 786
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 1 cut(s) 877
SduI GDGCHC 4 cut(s) 247, 297, 1443, 1682
SfaNI GCATC 4 cut(s) 393, 637, 643, 691
SfcI CTRYAG 2 cut(s) 657, 1495
SfoI GGCGCC 1 cut(s) 217
SmiMI CAYNNNNRTG 2 cut(s) 995, 1535
SmlI CTYRAG 2 cut(s) 1485, 1816
SmoI CTYRAG 2 cut(s) 1485, 1816
SphI GCATGC 1 cut(s) 1604
SsiI CCGC 7 cut(s) 363, 377, 669, 740, 1016, 1475, 1643
SspDI GGCGCC 1 cut(s) 215
SspMI CTAG 3 cut(s) 912, 1211, 1332
SstI GAGCTC 1 cut(s) 1682
StyD4I CCNGG 1 cut(s) 875
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 3 cut(s) 1271, 1447, 1548
TaiI ACGT 2 cut(s) 926, 1152
TaqI TCGA 6 cut(s) 291, 449, 534, 575, 826, 1146
TatI WGTACW 1 cut(s) 1783
TauI GCSGC 1 cut(s) 1019
Tru1I TTAA 3 cut(s) 1112, 1257, 1589
Tru9I TTAA 3 cut(s) 1112, 1257, 1589
TscAI CASTG 5 cut(s) 627, 978, 1276, 1450, 1762
TseFI GTSAC 3 cut(s) 102, 420, 562
TseI GCWGC 2 cut(s) 1348, 1497
Tsp45I GTSAC 3 cut(s) 102, 420, 562
TspGWI ACGGA 2 cut(s) 177, 1672
TspRI CASTG 5 cut(s) 627, 978, 1276, 1450, 1762
Van91I CCANNNNNTGG 1 cut(s) 1674
XapI RAATTY 3 cut(s) 445, 539, 907
XceI RCATGY 2 cut(s) 43, 1604
XspI CTAG 3 cut(s) 912, 1211, 1332
Zsp2I ATGCAT 2 cut(s) 750, 1378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.