pycom14g06540

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005549
Physical Location & Seq
Forward (+)
6476984 .. 6478021
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGTATCTCAGGATCGATCCCGTTACAGCGGTGCAACTGCTTTGGGTGAACATGATTATGGACACTCTTGGTGCTTTGGCACTGGCAACAGAGCCTCCAAATGATGGACTTATGAAAAGGCCCCCAGTTGGGAGGGGTACAAGCTTCATCACCAAGGCTATGTGGAGGAATATCATTGGTCAGAGTATCTATCAACTGGCTGTCCTTGGAGTTCTCAATTTCTCGGGGAAGAAGCTACTAGGACTTACTGGTTCAGATGCAACTGAGGTTCTTAACACTGTGATATTCAACGCATTCGTGTTTTGCCAGGTGTTTAATCAGATAAACAGTCGTGACATAGAGAAGATAAACATATTCCGGGGAATGTTTGATAGCTGGATATTCCTAGGCGTCATGGTTTGCACAGCAGTCTTCCAGGCTATCATAGTAGAGTTCTTGGGAGATTTTGCTAGCACTGTACCACTAAGCTGGCAACTATGGTTGCTTTGCATTTTACTTGGATCTGTTAGCATGCCTGTTGCAGTTGTGCTCAAATTAATACCTGTCGAAAGAACAATTAAGCACCATGACGGATATGAAGCACTTCCTTCTGGTCCTCCGGAGGGCATAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

205

Amino Acids

22.5

Weight (kDa)

6.09

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_C PF00689 7 - 180 2.3e-44 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 104
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 1 cut(s) 29
AclWI GGATC 3 cut(s) 11, 20, 508
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 2 cut(s) 139, 459
AfiI CCNNNNNNNGG 4 cut(s) 104, 128, 129, 602
AgsI TTSAA 1 cut(s) 289
AjnI CCWGG 2 cut(s) 306, 414
AluBI AGCT 4 cut(s) 144, 235, 375, 468
AluI AGCT 4 cut(s) 144, 235, 375, 468
Alw21I GWGCWC 1 cut(s) 531
AlwI GGATC 3 cut(s) 11, 20, 508
Ama87I CYCGRG 1 cut(s) 223
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 1 cut(s) 119
AseI ATTAAT 1 cut(s) 536
Asp700I GAANNNNTTC 1 cut(s) 582
AspA2I CCTAGG 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 120, 593
AsuC2I CCSGG 1 cut(s) 359
AsuHPI GGTGA 2 cut(s) 58, 142
AsuNHI GCTAGC 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 1 cut(s) 593
AvrII CCTAGG 1 cut(s) 385
BbsI GAAGAC 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 531
BccI CCATC 1 cut(s) 98
BciT130I CCWGG 2 cut(s) 308, 416
BcnI CCSGG 1 cut(s) 359
BfaI CTAG 3 cut(s) 239, 386, 450
BlnI CCTAGG 1 cut(s) 385
Bme1390I CCNGG 3 cut(s) 308, 359, 416
Bme18I GGWCC 1 cut(s) 593
BmeT110I CYCGRG 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 120, 593
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 3 cut(s) 308, 359, 416
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 1 cut(s) 247
BmtI GCTAGC 1 cut(s) 453
BmuI ACTGGG 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 403
BpuMI CCSGG 1 cut(s) 359
Bsa29I ATCGAT 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 4 cut(s) 153, 205, 358, 385
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 128, 129, 602
Bse1I ACTGG 4 cut(s) 87, 125, 201, 253
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 2 cut(s) 308, 416
BseCI ATCGAT 1 cut(s) 15
BseDI CCNNGG 4 cut(s) 153, 205, 358, 385
BseLI CCNNNNNNNGG 4 cut(s) 104, 128, 129, 602
BseMII CTCAG 2 cut(s) 22, 255
BseNI ACTGG 4 cut(s) 87, 125, 201, 253
BshFI GGCC 1 cut(s) 121
BshVI ATCGAT 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 531
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 2 cut(s) 358, 599
BslI CCNNNNNNNGG 4 cut(s) 104, 128, 129, 602
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 1 cut(s) 121
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 531
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 3 cut(s) 12, 16, 500
BspACI CCGC 1 cut(s) 29
BspANI GGCC 1 cut(s) 121
BspCNI CTCAG 2 cut(s) 21, 256
BspDI ATCGAT 1 cut(s) 15
BspEI TCCGGA 1 cut(s) 598
BspLI GGNNCC 1 cut(s) 122
BspOI GCTAGC 1 cut(s) 453
BspPI GGATC 3 cut(s) 11, 20, 508
BsrI ACTGG 4 cut(s) 87, 125, 201, 253
BssECI CCNNGG 4 cut(s) 153, 205, 358, 385
BssMI GATC 3 cut(s) 12, 16, 500
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 3 cut(s) 153, 205, 385
Bst2UI CCWGG 2 cut(s) 308, 416
Bst4CI ACNGT 3 cut(s) 280, 329, 457
BstACI GRCGYC 1 cut(s) 390
BstC8I GCNNGC 3 cut(s) 451, 470, 512
BstDEI CTNAG 3 cut(s) 8, 264, 464
BstKTI GATC 3 cut(s) 15, 19, 503
BstMBI GATC 3 cut(s) 12, 16, 500
BstNI CCWGG 2 cut(s) 308, 416
BstNSI RCATGY 1 cut(s) 514
BstSCI CCNGG 3 cut(s) 306, 357, 414
BstV2I GAAGAC 1 cut(s) 403
BstX2I RGATCY 1 cut(s) 500
BstXI CCANNNNNNTGG 1 cut(s) 468
BstYI RGATCY 1 cut(s) 500
Bsu15I ATCGAT 1 cut(s) 15
BsuRI GGCC 1 cut(s) 121
BsuTUI ATCGAT 1 cut(s) 15
BtsIMutI CAGTG 3 cut(s) 80, 276, 453
Cac8I GCNNGC 3 cut(s) 451, 470, 512
Cfr13I GGNCC 2 cut(s) 120, 593
ClaI ATCGAT 1 cut(s) 15
CseI GACGC 1 cut(s) 379
Csp6I GTAC 2 cut(s) 138, 458
CviAII CATG 4 cut(s) 52, 394, 511, 566
CviJI RGCY 9 cut(s) 94, 121, 144, 158, 200, 235, 375, 419, 468
CviKI_1 RGCY 9 cut(s) 94, 121, 144, 158, 200, 235, 375, 419, 468
CviQI GTAC 2 cut(s) 138, 458
DdeI CTNAG 3 cut(s) 8, 264, 464
DpnI GATC 3 cut(s) 14, 18, 502
DpnII GATC 3 cut(s) 12, 16, 500
Eco130I CCWWGG 3 cut(s) 153, 205, 385
Eco47I GGWCC 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 120
EcoRII CCWGG 2 cut(s) 306, 414
EcoT14I CCWWGG 3 cut(s) 153, 205, 385
ErhI CCWWGG 3 cut(s) 153, 205, 385
FaeI CATG 4 cut(s) 55, 397, 514, 569
FatI CATG 4 cut(s) 51, 393, 510, 565
FspBI CTAG 3 cut(s) 239, 386, 450
HaeIII GGCC 1 cut(s) 121
HapII CCGG 2 cut(s) 358, 599
HgaI GACGC 1 cut(s) 379
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 4 cut(s) 55, 397, 514, 569
HindIII AAGCTT 1 cut(s) 142
HpaII CCGG 2 cut(s) 358, 599
HphI GGTGA 2 cut(s) 58, 142
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 3 cut(s) 183, 256, 321
Hpy188III TCNNGA 3 cut(s) 10, 332, 599
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 597
HpyCH4III ACNGT 3 cut(s) 280, 329, 457
HpyCH4V TGCA 5 cut(s) 34, 260, 402, 489, 521
HpyF3I CTNAG 3 cut(s) 8, 264, 464
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 4 cut(s) 55, 397, 514, 569
Kpn2I TCCGGA 1 cut(s) 598
Kzo9I GATC 3 cut(s) 12, 16, 500
LweI GCATC 1 cut(s) 247
MaeI CTAG 3 cut(s) 239, 386, 450
MaeIII GTNAC 2 cut(s) 22, 332
MalI GATC 3 cut(s) 14, 18, 502
MboI GATC 3 cut(s) 12, 16, 500
MboII GAAGA 3 cut(s) 241, 355, 403
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 3 cut(s) 217, 533, 555
MnlI CCTC 6 cut(s) 105, 126, 159, 259, 595, 606
MroI TCCGGA 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 582
MseI TTAA 4 cut(s) 273, 315, 536, 558
MslI CAYNNNNRTG 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 2 cut(s) 358, 599
MspR9I CCNGG 3 cut(s) 308, 359, 416
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 2 cut(s) 308, 416
NciI CCSGG 1 cut(s) 359
NdeII GATC 3 cut(s) 12, 16, 500
NheI GCTAGC 1 cut(s) 449
NlaIII CATG 4 cut(s) 55, 397, 514, 569
NlaIV GGNNCC 1 cut(s) 122
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 1 cut(s) 514
PaeI GCATGC 1 cut(s) 514
PctI GAATGC 1 cut(s) 293
PdmI GAANNNNTTC 1 cut(s) 582
PflMI CCANNNNNTGG 1 cut(s) 104
PshBI ATTAAT 1 cut(s) 536
Psp6I CCWGG 2 cut(s) 306, 414
PspGI CCWGG 2 cut(s) 306, 414
PspN4I GGNNCC 1 cut(s) 122
PspPI GGNCC 2 cut(s) 120, 593
PsuI RGATCY 1 cut(s) 500
RsaI GTAC 2 cut(s) 139, 459
RsaNI GTAC 2 cut(s) 138, 458
RseI CAYNNNNRTG 1 cut(s) 56
SaqAI TTAA 4 cut(s) 273, 315, 536, 558
Sau3AI GATC 3 cut(s) 12, 16, 500
Sau96I GGNCC 2 cut(s) 120, 593
ScrFI CCNGG 3 cut(s) 308, 359, 416
SduI GDGCHC 1 cut(s) 531
SetI ASST 7 cut(s) 146, 237, 270, 312, 377, 470, 544
SfaNI GCATC 1 cut(s) 247
SinI GGWCC 1 cut(s) 593
SmiMI CAYNNNNRTG 1 cut(s) 56
SphI GCATGC 1 cut(s) 514
Sse9I AATT 3 cut(s) 217, 533, 555
SsiI CCGC 1 cut(s) 29
SspMI CTAG 3 cut(s) 239, 386, 450
StyD4I CCNGG 3 cut(s) 306, 357, 414
StyI CCWWGG 3 cut(s) 153, 205, 385
TaaI ACNGT 3 cut(s) 280, 329, 457
TaqI TCGA 2 cut(s) 15, 546
TasI AATT 3 cut(s) 217, 533, 555
Tru1I TTAA 4 cut(s) 273, 315, 536, 558
Tru9I TTAA 4 cut(s) 273, 315, 536, 558
TscAI CASTG 3 cut(s) 87, 283, 460
TseFI GTSAC 1 cut(s) 332
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 3 cut(s) 128, 136, 591
TspGWI ACGGA 1 cut(s) 585
TspRI CASTG 3 cut(s) 87, 283, 460
Van91I CCANNNNNTGG 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 593
VspI ATTAAT 1 cut(s) 536
XceI RCATGY 1 cut(s) 514
XmaJI CCTAGG 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 3 cut(s) 239, 386, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.