Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0003551

Physical Location & Seq

Genomic Coordinates Forward (+)

16735447 .. 16736834

Gene Structure 1388 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGACTAAGTTTGTTTGGGTTGCACTTGCTCTCTCGCTCGCTTTGGTGATTGGGGTTTCTGAGAGTATTGATTACCATGAGAAGGACTTGGCCTCGGAGGAGAGCTTGTGGGACTTGTATGAGAGGTGGAGGAGCCATCACACGGTTTCCAGGAGCCTTGACGAGAAGCACAAGCGGTTCAATGTGTTTAAAGAGAATGTCAAGCATGTTCACAACACTAACAAAGGTGATAAGCCTTACAAGCTGAAGCTCAACAAGTTTGCTGACATGACCAACCATGAGTTCAGGAGTGTTTACGCTGGTTCGAAGGTTAAGCACCATAGAATGCTCCGAGGGCAGCGCGCTGACAGAGGCTTTCGGTATGCAAATGTTGAGAGTGTCCCACCGTCTGTTGATTGGAGAAGGAATGGTGCTGTCACTCCCATTAAGGATCAAGGCCAATGCGGAAGTTGCTGGGCGTTTTCGACAGTTGCAGCTGTTGAGGGCATCAATCAAATTAAGACAAAGGAGCTAGTTTCGTTGTCTGAGCAAGAGCTCGTCGACTGTGACACGGAACAAAACCAAGGATGCAATGGAGGGCTAATGGAACTGGCAATGGAGTTCATCAAACAAAAGGGAGGCCTGACAACCGAAATTAATTACCCTTACCGAGCAGCAGATTCAACATGTAATGTTGCAAAGGAAAACGCTCCGGTTGTGTCCATTGATGGCCATGAGAGTGTGCCTGCTAATGACGAGGATGCATTGCTCAAAGCAGCTGCAAACCAACCTATTGCTGTCGCCATCGATGCTGGAGGTTCCGATTTCCAATTCTACGATGAGGGGGTATTTGACGGGAAATGCGGCATAGAGCTTGATCACGGAGTGGCAGTTGTTGGCTACGGAACAACACTTGATGGAACCAAGTATTGGATAGTCAAGAACTCATGGGGACCCGAGTGGGGAGAGAAGGGTTACATAAGGATGCAGCGCGGCGTCTCTGCTAAGGAAGGGATCTGTGGAATAGCTATGGAGGCTTCGTATCCCGTCAAGAACTCTTCGACAAACCCTAAAGCAGCAGCTACCTCAAATCCTAAGGACGAACTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005788 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0031974 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0070013 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

363

Amino Acids

40.07

Weight (kDa)

6.03

Isoelectric Point (pI)

28.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	40 - 95	1e-15	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	127 - 343	1.6e-83	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003551)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48340 AT3G48350 AT3G48350 AT5G50260
fragaria_vesca	FvH4_5g06770 FvH4_5g06770
malus_domestica	MD06G1161000.v1.1 MD13G1105500.v1.1 MD14G1166600.v1.1 MD16G1105700.v1.1
prunus_persica	Prupe.1G240900_v2.0.a1 Prupe.5G160900_v2.0.a1
pyrus_communis	pycom06g14380 pycom14g13920
rosa_chinensis	RchiOBHm_Chr4g0432451 RchiOBHm_Chr7g0191791
rosa_laevigata	RLG00000004423 RLG00000006841
rosa_multiflora	Rmu_sc0041316.1_g000001 Rmu_ssc0000421.1_g000001
rosa_roxburghii	Rroxscaffold_3G00263520 Rroxscaffold_5G00373930
rosa_rugosa	Rorug04G0259100.1 Rorug04G0259200.1 Rorug04G0259300 Rorug04G0259400 Rorug06G0511400
rosa_samantha	Rh4AG314400 Rh4BG322200 Rh4CG337300 Rh7AG121500 Rh7BG122900 Rh7CG125800
rosa_wichuraiana	Rw4G027340 Rw7G010330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	909
AccI	GTMKAC	1 cut(s)	540
AccII	CGCG	2 cut(s)	342, 972
AciI	CCGC	4 cut(s)	175, 444, 843, 972
AclWI	GGATC	2 cut(s)	438, 1001
AcoI	YGGCCR	1 cut(s)	709
AcuI	CTGAAG	1 cut(s)	266
AcyI	GRCGYC	1 cut(s)	975
AdeI	CACNNNGTG	1 cut(s)	865
AfiI	CCNNNNNNNGG	4 cut(s)	82, 142, 909, 941
AflIII	ACRYGT	1 cut(s)	665
AgsI	TTSAA	2 cut(s)	181, 663
AjnI	CCWGG	1 cut(s)	149
AjuI	GAANNNNNNNTTGG	4 cut(s)	266, 298, 892, 924
Alw21I	GWGCWC	1 cut(s)	537
Alw26I	GTCTC	1 cut(s)	982
AlwI	GGATC	2 cut(s)	438, 1001
Ama87I	CYCGRG	1 cut(s)	935
AoxI	GGCC	4 cut(s)	90, 436, 619, 709
ApeKI	GCWGC	8 cut(s)	337, 473, 653, 755, 758, 967, 1055, 1058
AseI	ATTAAT	1 cut(s)	636
AspLEI	GCGC	3 cut(s)	342, 344, 972
AspS9I	GGNCC	1 cut(s)	932
AsuHPI	GGTGA	2 cut(s)	58, 239
AsuII	TTCGAA	1 cut(s)	305
AvaI	CYCGRG	1 cut(s)	935
AvaII	GGWCC	1 cut(s)	932
AxyI	CCTNAGG	1 cut(s)	1074
BalI	TGGCCA	1 cut(s)	711
BanII	GRGCYC	1 cut(s)	537
Bbv12I	GWGCWC	1 cut(s)	537
BbvI	GCAGC	8 cut(s)	349, 485, 665, 745, 767, 979, 1067, 1070
BccI	CCATC	4 cut(s)	144, 701, 791, 890
BciT130I	CCWGG	1 cut(s)	151
BciVI	GTATCC	1 cut(s)	1032
BclI	TGATCA	1 cut(s)	856
BcoDI	GTCTC	1 cut(s)	982
BfaI	CTAG	1 cut(s)	512
BfuI	GTATCC	1 cut(s)	1032
Bme1390I	CCNGG	1 cut(s)	151
Bme18I	GGWCC	1 cut(s)	932
BmeT110I	CYCGRG	1 cut(s)	935
BmgT120I	GGNCC	1 cut(s)	932
BmiI	GGNNCC	6 cut(s)	134, 155, 799, 901, 933, 934
BmrFI	CCNGG	1 cut(s)	151
BmsI	GCATC	5 cut(s)	495, 557, 730, 778, 954
BpmI	CTGGAG	1 cut(s)	813
Bpu10I	CCTNAGC	1 cut(s)	984
Bpu14I	TTCGAA	1 cut(s)	305
Bsa29I	ATCGAT	1 cut(s)	786
BsaHI	GRCGYC	1 cut(s)	975
BsaJI	CCNNGG	3 cut(s)	93, 331, 562
BsaWI	WCCGGW	1 cut(s)	691
BsaXI	ACNNNNNCTCC	6 cut(s)	92, 122, 855, 885, 1004, 1034
Bsc4I	CCNNNNNNNGG	4 cut(s)	82, 142, 909, 941
Bse1I	ACTGG	1 cut(s)	594
Bse21I	CCTNAGG	1 cut(s)	1074
Bse3DI	GCAATG	3 cut(s)	577, 600, 743
BseBI	CCWGG	1 cut(s)	151
BseCI	ATCGAT	1 cut(s)	786
BseDI	CCNNGG	3 cut(s)	93, 331, 562
BseGI	GGATG	3 cut(s)	572, 745, 969
BseLI	CCNNNNNNNGG	4 cut(s)	82, 142, 909, 941
BseMI	GCAATG	3 cut(s)	577, 600, 743
BseMII	CTCAG	2 cut(s)	51, 516
BseNI	ACTGG	1 cut(s)	594
BsePI	GCGCGC	1 cut(s)	340
BseRI	GAGGAG	2 cut(s)	113, 145
BseXI	GCAGC	8 cut(s)	349, 485, 665, 745, 767, 979, 1067, 1070
BseYI	CCCAGC	1 cut(s)	453
Bsh1236I	CGCG	2 cut(s)	342, 972
BshFI	GGCC	4 cut(s)	92, 438, 621, 711
BshVI	ATCGAT	1 cut(s)	786
BsiHKAI	GWGCWC	1 cut(s)	537
BsiHKCI	CYCGRG	1 cut(s)	935
BsiSI	CCGG	1 cut(s)	692
BslFI	GGGAC	3 cut(s)	125, 365, 945
BslI	CCNNNNNNNGG	4 cut(s)	82, 142, 909, 941
BsmAI	GTCTC	1 cut(s)	982
BsmBI	CGTCTC	1 cut(s)	982
BsmFI	GGGAC	3 cut(s)	125, 365, 945
BsmI	GAATGC	1 cut(s)	330
BsnI	GGCC	4 cut(s)	92, 438, 621, 711
BsoBI	CYCGRG	1 cut(s)	935
Bsp119I	TTCGAA	1 cut(s)	305
Bsp1286I	GDGCHC	1 cut(s)	537
Bsp143I	GATC	3 cut(s)	430, 856, 993
BspACI	CCGC	4 cut(s)	175, 444, 843, 972
BspANI	GGCC	4 cut(s)	92, 438, 621, 711
BspCNI	CTCAG	2 cut(s)	52, 517
BspDI	ATCGAT	1 cut(s)	786
BspFNI	CGCG	2 cut(s)	342, 972
BspLI	GGNNCC	6 cut(s)	134, 155, 799, 901, 933, 934
BspPI	GGATC	2 cut(s)	438, 1001
BspT104I	TTCGAA	1 cut(s)	305
BsrDI	GCAATG	3 cut(s)	577, 600, 743
BsrI	ACTGG	1 cut(s)	594
BssECI	CCNNGG	3 cut(s)	93, 331, 562
BssHII	GCGCGC	1 cut(s)	340
BssMI	GATC	3 cut(s)	430, 856, 993
BssNI	GRCGYC	1 cut(s)	975
BssT1I	CCWWGG	1 cut(s)	562
Bst2UI	CCWGG	1 cut(s)	151
Bst4CI	ACNGT	4 cut(s)	145, 387, 469, 545
Bst6I	CTCTTC	1 cut(s)	1042
BstACI	GRCGYC	1 cut(s)	975
BstBI	TTCGAA	1 cut(s)	305
BstC8I	GCNNGC	3 cut(s)	39, 342, 726
BstDEI	CTNAG	5 cut(s)	6, 60, 525, 984, 1074
BstF5I	GGATG	3 cut(s)	572, 745, 969
BstFNI	CGCG	2 cut(s)	342, 972
BstHHI	GCGC	3 cut(s)	342, 344, 972
BstKTI	GATC	3 cut(s)	433, 859, 996
BstMAI	GTCTC	1 cut(s)	982
BstMBI	GATC	3 cut(s)	430, 856, 993
BstMWI	GCNNNNNNNGC	5 cut(s)	241, 334, 450, 788, 1013
BstNI	CCWGG	1 cut(s)	151
BstNSI	RCATGY	2 cut(s)	209, 669
BstSCI	CCNGG	1 cut(s)	149
BstUI	CGCG	2 cut(s)	342, 972
BstV1I	GCAGC	8 cut(s)	349, 485, 665, 745, 767, 979, 1067, 1070
BstX2I	RGATCY	1 cut(s)	993
BstYI	RGATCY	1 cut(s)	993
Bsu15I	ATCGAT	1 cut(s)	786
Bsu36I	CCTNAGG	1 cut(s)	1074
BsuI	GTATCC	1 cut(s)	1032
BsuRI	GGCC	4 cut(s)	92, 438, 621, 711
BsuTUI	ATCGAT	1 cut(s)	786
BtsCI	GGATG	3 cut(s)	572, 745, 969
Cac8I	GCNNGC	3 cut(s)	39, 342, 726
CfoI	GCGC	3 cut(s)	342, 344, 972
Cfr13I	GGNCC	1 cut(s)	932
ClaI	ATCGAT	1 cut(s)	786
CseI	GACGC	1 cut(s)	964
CviAII	CATG	7 cut(s)	77, 206, 268, 278, 666, 713, 927
DdeI	CTNAG	5 cut(s)	6, 60, 525, 984, 1074
DpnI	GATC	3 cut(s)	432, 858, 995
DpnII	GATC	3 cut(s)	430, 856, 993
DraI	TTTAAA	1 cut(s)	190
DraIII	CACNNNGTG	1 cut(s)	865
EaeI	YGGCCR	1 cut(s)	709
Eam1104I	CTCTTC	1 cut(s)	1042
EarI	CTCTTC	1 cut(s)	1042
Ecl136II	GAGCTC	1 cut(s)	535
Eco130I	CCWWGG	1 cut(s)	562
Eco147I	AGGCCT	1 cut(s)	621
Eco24I	GRGCYC	1 cut(s)	537
Eco47I	GGWCC	1 cut(s)	932
Eco53kI	GAGCTC	1 cut(s)	535
Eco57I	CTGAAG	1 cut(s)	266
Eco81I	CCTNAGG	1 cut(s)	1074
Eco88I	CYCGRG	1 cut(s)	935
EcoICRI	GAGCTC	1 cut(s)	535
EcoO109I	RGGNCCY	1 cut(s)	932
EcoRII	CCWGG	1 cut(s)	149
EcoT14I	CCWWGG	1 cut(s)	562
EcoT22I	ATGCAT	1 cut(s)	745
EcoT38I	GRGCYC	1 cut(s)	537
ErhI	CCWWGG	1 cut(s)	562
Esp3I	CGTCTC	1 cut(s)	982
FaeI	CATG	7 cut(s)	80, 209, 271, 281, 669, 716, 930
FaqI	GGGAC	3 cut(s)	125, 365, 945
FatI	CATG	7 cut(s)	76, 205, 267, 277, 665, 712, 926
FbaI	TGATCA	1 cut(s)	856
FblI	GTMKAC	1 cut(s)	540
FokI	GGATG	3 cut(s)	579, 752, 976
FriOI	GRGCYC	1 cut(s)	537
FspBI	CTAG	1 cut(s)	512
GlaI	GCGC	3 cut(s)	341, 343, 971
GsaI	CCCAGC	1 cut(s)	457
GsuI	CTGGAG	1 cut(s)	813
HaeIII	GGCC	4 cut(s)	92, 438, 621, 711
HapII	CCGG	1 cut(s)	692
HgaI	GACGC	1 cut(s)	964
HhaI	GCGC	3 cut(s)	342, 344, 972
Hin1I	GRCGYC	1 cut(s)	975
Hin1II	CATG	7 cut(s)	80, 209, 271, 281, 669, 716, 930
Hin6I	GCGC	3 cut(s)	340, 342, 970
HinP1I	GCGC	3 cut(s)	340, 342, 970
HincII	GTYRAC	1 cut(s)	541
HindII	GTYRAC	1 cut(s)	541
HinfI	GANTC	1 cut(s)	659
HpaII	CCGG	1 cut(s)	692
HphI	GGTGA	2 cut(s)	58, 239
Hpy166II	GTNNAC	3 cut(s)	211, 295, 541
Hpy188I	TCNGA	5 cut(s)	61, 97, 332, 526, 802
Hpy188III	TCNNGA	3 cut(s)	286, 919, 1030
Hpy8I	GTNNAC	3 cut(s)	211, 295, 541
Hpy99I	CGWCG	1 cut(s)	542
HpyAV	CCTTC	5 cut(s)	76, 301, 396, 943, 983
HpyCH4III	ACNGT	4 cut(s)	145, 387, 469, 545
HpyCH4V	TGCA	8 cut(s)	23, 365, 473, 570, 677, 743, 761, 967
HpyF10VI	GCNNNNNNNGC	5 cut(s)	241, 334, 450, 788, 1013
HpyF3I	CTNAG	5 cut(s)	6, 60, 525, 984, 1074
Hsp92I	GRCGYC	1 cut(s)	975
Hsp92II	CATG	7 cut(s)	80, 209, 271, 281, 669, 716, 930
HspAI	GCGC	3 cut(s)	340, 342, 970
KflI	GGGWCCC	1 cut(s)	932
Ksp22I	TGATCA	1 cut(s)	856
Kzo9I	GATC	3 cut(s)	430, 856, 993
LmnI	GCTCC	5 cut(s)	132, 153, 333, 508, 694
Lsp1109I	GCAGC	8 cut(s)	349, 485, 665, 745, 767, 979, 1067, 1070
LweI	GCATC	5 cut(s)	495, 557, 730, 778, 954
MaeI	CTAG	1 cut(s)	512
MaeIII	GTNAC	3 cut(s)	415, 545, 953
MalI	GATC	3 cut(s)	432, 858, 995
MboI	GATC	3 cut(s)	430, 856, 993
MboII	GAAGA	1 cut(s)	1029
MflI	RGATCY	1 cut(s)	993
MhlI	GDGCHC	1 cut(s)	537
MlsI	TGGCCA	1 cut(s)	711
MluCI	AATT	4 cut(s)	495, 633, 637, 809
MluNI	TGGCCA	1 cut(s)	711
Mox20I	TGGCCA	1 cut(s)	711
Mph1103I	ATGCAT	1 cut(s)	745
MscI	TGGCCA	1 cut(s)	711
MseI	TTAA	5 cut(s)	189, 312, 426, 498, 636
MslI	CAYNNNNRTG	2 cut(s)	717, 962
Msp20I	TGGCCA	1 cut(s)	711
MspA1I	CMGCKG	2 cut(s)	476, 758
MspI	CCGG	1 cut(s)	692
MspR9I	CCNGG	1 cut(s)	151
Mva1269I	GAATGC	1 cut(s)	330
MvaI	CCWGG	1 cut(s)	151
MvnI	CGCG	2 cut(s)	342, 972
MwoI	GCNNNNNNNGC	5 cut(s)	241, 334, 450, 788, 1013
NdeII	GATC	3 cut(s)	430, 856, 993
NlaIII	CATG	7 cut(s)	80, 209, 271, 281, 669, 716, 930
NlaIV	GGNNCC	6 cut(s)	134, 155, 799, 901, 933, 934
NmuCI	GTSAC	2 cut(s)	415, 545
NsiI	ATGCAT	1 cut(s)	745
NspI	RCATGY	2 cut(s)	209, 669
NspV	TTCGAA	1 cut(s)	305
PauI	GCGCGC	1 cut(s)	340
PceI	AGGCCT	1 cut(s)	621
PciI	ACATGT	1 cut(s)	665
PctI	GAATGC	1 cut(s)	330
PfeI	GAWTC	1 cut(s)	659
PflMI	CCANNNNNTGG	1 cut(s)	909
PfoI	TCCNGGA	1 cut(s)	149
PpuMI	RGGWCCY	1 cut(s)	932
PscI	ACATGT	1 cut(s)	665
PshBI	ATTAAT	1 cut(s)	636
Psp124BI	GAGCTC	1 cut(s)	537
Psp5II	RGGWCCY	1 cut(s)	932
Psp6I	CCWGG	1 cut(s)	149
PspFI	CCCAGC	1 cut(s)	453
PspGI	CCWGG	1 cut(s)	149
PspN4I	GGNNCC	6 cut(s)	134, 155, 799, 901, 933, 934
PspPI	GGNCC	1 cut(s)	932
PspPPI	RGGWCCY	1 cut(s)	932
PsuI	RGATCY	1 cut(s)	993
PteI	GCGCGC	1 cut(s)	340
PvuII	CAGCTG	2 cut(s)	476, 758
RseI	CAYNNNNRTG	2 cut(s)	717, 962
SacI	GAGCTC	1 cut(s)	537
SalI	GTCGAC	1 cut(s)	539
SaqAI	TTAA	5 cut(s)	189, 312, 426, 498, 636
Sau3AI	GATC	3 cut(s)	430, 856, 993
Sau96I	GGNCC	1 cut(s)	932
ScrFI	CCNGG	1 cut(s)	151
SduI	GDGCHC	1 cut(s)	537
SfaNI	GCATC	5 cut(s)	495, 557, 730, 778, 954
SfuI	TTCGAA	1 cut(s)	305
SinI	GGWCC	1 cut(s)	932
SmiMI	CAYNNNNRTG	2 cut(s)	717, 962
Sse9I	AATT	4 cut(s)	495, 633, 637, 809
SseBI	AGGCCT	1 cut(s)	621
SsiI	CCGC	4 cut(s)	175, 444, 843, 972
SspMI	CTAG	1 cut(s)	512
SstI	GAGCTC	1 cut(s)	537
StuI	AGGCCT	1 cut(s)	621
StyD4I	CCNGG	1 cut(s)	149
StyI	CCWWGG	1 cut(s)	562
TaaI	ACNGT	4 cut(s)	145, 387, 469, 545
TaqI	TCGA	5 cut(s)	305, 464, 540, 786, 1040
TasI	AATT	4 cut(s)	495, 633, 637, 809
TauI	GCSGC	2 cut(s)	846, 975
TfiI	GAWTC	1 cut(s)	659
Tru1I	TTAA	5 cut(s)	189, 312, 426, 498, 636
Tru9I	TTAA	5 cut(s)	189, 312, 426, 498, 636
TseFI	GTSAC	2 cut(s)	415, 545
TseI	GCWGC	8 cut(s)	337, 473, 653, 755, 758, 967, 1055, 1058
Tsp45I	GTSAC	2 cut(s)	415, 545
TspDTI	ATGAA	1 cut(s)	592
TspGWI	ACGGA	3 cut(s)	566, 876, 897
Van91I	CCANNNNNTGG	1 cut(s)	909
VpaK11BI	GGWCC	1 cut(s)	932
VspI	ATTAAT	1 cut(s)	636
XceI	RCATGY	2 cut(s)	209, 669
XcmI	CCANNNNNNNNNTGG	1 cut(s)	569
XmiI	GTMKAC	1 cut(s)	540
XspI	CTAG	1 cut(s)	512
Zsp2I	ATGCAT	1 cut(s)	745

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.